Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS21580 and ABZR87_RS21585 are separated by 332 nucleotides ABZR87_RS21585 and ABZR87_RS21590 are separated by 20 nucleotides ABZR87_RS21590 and ABZR87_RS21595 are separated by 74 nucleotides
ABZR87_RS21580: ABZR87_RS21580 - HAD-IA family hydrolase, at 1,224,664 to 1,225,362
_RS21580
ABZR87_RS21585: ABZR87_RS21585 - multidrug efflux SMR transporter, at 1,225,695 to 1,226,024
_RS21585
ABZR87_RS21590: ABZR87_RS21590 - NAD(P)H-dependent oxidoreductase, at 1,226,045 to 1,226,689
_RS21590
ABZR87_RS21595: ABZR87_RS21595 - Rrf2 family transcriptional regulator, at 1,226,764 to 1,227,201
_RS21595
Position (kb)
1225
1226
1227 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1224.705 kb on + strand at 1224.706 kb on - strand at 1224.706 kb on - strand at 1224.850 kb on + strand, within ABZR87_RS21580 at 1224.850 kb on + strand, within ABZR87_RS21580 at 1224.850 kb on + strand, within ABZR87_RS21580 at 1224.850 kb on + strand, within ABZR87_RS21580 at 1224.851 kb on - strand, within ABZR87_RS21580 at 1224.851 kb on - strand, within ABZR87_RS21580 at 1224.851 kb on - strand, within ABZR87_RS21580 at 1224.887 kb on + strand, within ABZR87_RS21580 at 1224.887 kb on + strand, within ABZR87_RS21580 at 1224.902 kb on + strand, within ABZR87_RS21580 at 1224.903 kb on - strand, within ABZR87_RS21580 at 1225.226 kb on + strand, within ABZR87_RS21580 at 1225.284 kb on - strand, within ABZR87_RS21580 at 1225.284 kb on - strand, within ABZR87_RS21580 at 1225.284 kb on - strand, within ABZR87_RS21580 at 1225.434 kb on - strand at 1225.439 kb on - strand at 1225.593 kb on + strand at 1225.593 kb on + strand at 1225.594 kb on - strand at 1225.594 kb on - strand at 1225.607 kb on + strand at 1225.607 kb on + strand at 1225.608 kb on - strand at 1225.608 kb on - strand at 1225.608 kb on - strand at 1225.608 kb on - strand at 1225.608 kb on - strand at 1225.634 kb on - strand at 1225.879 kb on - strand, within ABZR87_RS21585 at 1225.879 kb on - strand, within ABZR87_RS21585 at 1226.060 kb on + strand at 1226.060 kb on + strand at 1226.060 kb on + strand at 1226.061 kb on - strand at 1226.061 kb on - strand at 1226.130 kb on - strand, within ABZR87_RS21590 at 1226.324 kb on + strand, within ABZR87_RS21590 at 1226.324 kb on + strand, within ABZR87_RS21590 at 1226.325 kb on - strand, within ABZR87_RS21590 at 1226.325 kb on - strand, within ABZR87_RS21590 at 1226.474 kb on + strand, within ABZR87_RS21590 at 1226.509 kb on - strand, within ABZR87_RS21590 at 1226.547 kb on - strand, within ABZR87_RS21590 at 1226.547 kb on - strand, within ABZR87_RS21590 at 1226.547 kb on - strand, within ABZR87_RS21590 at 1226.734 kb on + strand at 1226.758 kb on - strand at 1226.769 kb on + strand at 1226.769 kb on + strand at 1226.770 kb on - strand at 1226.770 kb on - strand at 1226.770 kb on - strand at 1226.770 kb on - strand at 1226.799 kb on + strand at 1227.002 kb on + strand, within ABZR87_RS21595
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 1,224,705 + +0.3 1,224,706 - +0.7 1,224,706 - +0.4 1,224,850 + ABZR87_RS21580 0.27 -1.6 1,224,850 + ABZR87_RS21580 0.27 -1.4 1,224,850 + ABZR87_RS21580 0.27 +0.4 1,224,850 + ABZR87_RS21580 0.27 +0.4 1,224,851 - ABZR87_RS21580 0.27 -1.5 1,224,851 - ABZR87_RS21580 0.27 -0.3 1,224,851 - ABZR87_RS21580 0.27 -1.0 1,224,887 + ABZR87_RS21580 0.32 -0.1 1,224,887 + ABZR87_RS21580 0.32 -0.7 1,224,902 + ABZR87_RS21580 0.34 -0.8 1,224,903 - ABZR87_RS21580 0.34 -0.3 1,225,226 + ABZR87_RS21580 0.80 -1.0 1,225,284 - ABZR87_RS21580 0.89 +0.5 1,225,284 - ABZR87_RS21580 0.89 +0.8 1,225,284 - ABZR87_RS21580 0.89 -0.0 1,225,434 - -0.5 1,225,439 - +0.3 1,225,593 + -0.1 1,225,593 + +0.0 1,225,594 - -0.7 1,225,594 - +0.0 1,225,607 + -0.1 1,225,607 + +0.2 1,225,608 - +1.6 1,225,608 - -0.6 1,225,608 - -0.1 1,225,608 - -0.4 1,225,608 - -0.8 1,225,634 - -0.2 1,225,879 - ABZR87_RS21585 0.56 -1.2 1,225,879 - ABZR87_RS21585 0.56 -0.6 1,226,060 + +2.4 1,226,060 + -1.0 1,226,060 + +0.0 1,226,061 - -2.7 1,226,061 - -0.9 1,226,130 - ABZR87_RS21590 0.13 -0.6 1,226,324 + ABZR87_RS21590 0.43 +1.2 1,226,324 + ABZR87_RS21590 0.43 -0.1 1,226,325 - ABZR87_RS21590 0.43 -0.5 1,226,325 - ABZR87_RS21590 0.43 +0.4 1,226,474 + ABZR87_RS21590 0.67 -0.1 1,226,509 - ABZR87_RS21590 0.72 -0.8 1,226,547 - ABZR87_RS21590 0.78 +0.2 1,226,547 - ABZR87_RS21590 0.78 -0.5 1,226,547 - ABZR87_RS21590 0.78 -0.7 1,226,734 + +1.5 1,226,758 - -1.4 1,226,769 + +1.2 1,226,769 + +0.6 1,226,770 - +0.8 1,226,770 - -0.6 1,226,770 - +0.9 1,226,770 - +0.5 1,226,799 + -0.6 1,227,002 + ABZR87_RS21595 0.54 -0.2
Or see this region's nucleotide sequence