Strain Fitness in Ralstonia sp. UNC404CL21Col around ABZR87_RS20525

Experiment: Acanthamoeba sp., muxed culture-2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABZR87_RS20515 and ABZR87_RS20520 are separated by 32 nucleotidesABZR87_RS20520 and ABZR87_RS20525 are separated by 28 nucleotidesABZR87_RS20525 and ABZR87_RS20530 are separated by 145 nucleotides ABZR87_RS20515: ABZR87_RS20515 - ferredoxin FdxA, at 990,080 to 990,418 _RS20515 ABZR87_RS20520: ABZR87_RS20520 - crotonase/enoyl-CoA hydratase family protein, at 990,451 to 991,272 _RS20520 ABZR87_RS20525: ABZR87_RS20525 - Mpo1-like protein, at 991,301 to 991,834 _RS20525 ABZR87_RS20530: ABZR87_RS20530 - Crp/Fnr family transcriptional regulator, at 991,980 to 992,711 _RS20530 Position (kb) 991 992Strain fitness (log2 ratio) -2 -1 0 1 2 3at 990.645 kb on + strand, within ABZR87_RS20520at 990.745 kb on + strand, within ABZR87_RS20520at 990.746 kb on - strand, within ABZR87_RS20520at 990.746 kb on - strand, within ABZR87_RS20520at 990.746 kb on - strand, within ABZR87_RS20520at 990.809 kb on - strand, within ABZR87_RS20520at 990.850 kb on + strand, within ABZR87_RS20520at 990.850 kb on + strand, within ABZR87_RS20520at 990.851 kb on - strand, within ABZR87_RS20520at 990.970 kb on + strand, within ABZR87_RS20520at 990.970 kb on + strand, within ABZR87_RS20520at 990.970 kb on + strand, within ABZR87_RS20520at 991.271 kb on - strandat 991.283 kb on + strandat 991.283 kb on + strandat 991.476 kb on + strand, within ABZR87_RS20525at 991.476 kb on + strand, within ABZR87_RS20525at 991.476 kb on + strand, within ABZR87_RS20525at 991.477 kb on - strand, within ABZR87_RS20525at 991.477 kb on - strand, within ABZR87_RS20525at 991.479 kb on + strand, within ABZR87_RS20525at 991.479 kb on + strand, within ABZR87_RS20525at 991.479 kb on + strand, within ABZR87_RS20525at 991.526 kb on - strand, within ABZR87_RS20525at 991.638 kb on + strand, within ABZR87_RS20525at 991.638 kb on + strand, within ABZR87_RS20525at 991.638 kb on + strand, within ABZR87_RS20525at 991.638 kb on + strand, within ABZR87_RS20525at 991.639 kb on - strand, within ABZR87_RS20525at 991.639 kb on - strand, within ABZR87_RS20525at 991.775 kb on - strand, within ABZR87_RS20525at 991.775 kb on - strand, within ABZR87_RS20525at 991.803 kb on + strandat 991.804 kb on - strandat 991.804 kb on - strandat 991.804 kb on - strandat 991.888 kb on + strandat 991.888 kb on + strandat 991.889 kb on - strandat 991.889 kb on - strandat 991.889 kb on - strandat 991.904 kb on + strandat 992.026 kb on - strandat 992.166 kb on + strand, within ABZR87_RS20530at 992.272 kb on + strand, within ABZR87_RS20530at 992.273 kb on - strand, within ABZR87_RS20530at 992.318 kb on + strand, within ABZR87_RS20530at 992.333 kb on + strand, within ABZR87_RS20530at 992.333 kb on + strand, within ABZR87_RS20530at 992.333 kb on + strand, within ABZR87_RS20530at 992.333 kb on + strand, within ABZR87_RS20530at 992.334 kb on - strand, within ABZR87_RS20530at 992.488 kb on + strand, within ABZR87_RS20530at 992.488 kb on + strand, within ABZR87_RS20530at 992.488 kb on + strand, within ABZR87_RS20530at 992.488 kb on + strand, within ABZR87_RS20530at 992.489 kb on - strand, within ABZR87_RS20530at 992.489 kb on - strand, within ABZR87_RS20530at 992.489 kb on - strand, within ABZR87_RS20530at 992.489 kb on - strand, within ABZR87_RS20530at 992.489 kb on - strand, within ABZR87_RS20530at 992.691 kb on + strandat 992.691 kb on + strandat 992.692 kb on - strandat 992.692 kb on - strandat 992.724 kb on + strandat 992.725 kb on - strandat 992.725 kb on - strandat 992.730 kb on + strandat 992.730 kb on + strandat 992.756 kb on + strandat 992.756 kb on + strandat 992.756 kb on + strandat 992.756 kb on + strandat 992.756 kb on + strandat 992.756 kb on + strandat 992.756 kb on + strandat 992.756 kb on + strandat 992.756 kb on + strandat 992.756 kb on + strandat 992.757 kb on - strandat 992.757 kb on - strandat 992.757 kb on - strandat 992.757 kb on - strandat 992.758 kb on + strandat 992.758 kb on + strandat 992.758 kb on + strandat 992.758 kb on + strandat 992.759 kb on - strandat 992.759 kb on - strandat 992.759 kb on - strandat 992.759 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2
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990,645 + ABZR87_RS20520 0.24 +0.1
990,745 + ABZR87_RS20520 0.36 -1.0
990,746 - ABZR87_RS20520 0.36 -0.4
990,746 - ABZR87_RS20520 0.36 +0.5
990,746 - ABZR87_RS20520 0.36 +0.2
990,809 - ABZR87_RS20520 0.44 +0.3
990,850 + ABZR87_RS20520 0.49 +0.1
990,850 + ABZR87_RS20520 0.49 +0.3
990,851 - ABZR87_RS20520 0.49 +0.3
990,970 + ABZR87_RS20520 0.63 +0.6
990,970 + ABZR87_RS20520 0.63 +0.9
990,970 + ABZR87_RS20520 0.63 -0.2
991,271 - +0.5
991,283 + +0.7
991,283 + -0.1
991,476 + ABZR87_RS20525 0.33 +0.3
991,476 + ABZR87_RS20525 0.33 +0.0
991,476 + ABZR87_RS20525 0.33 -0.2
991,477 - ABZR87_RS20525 0.33 -1.2
991,477 - ABZR87_RS20525 0.33 -0.1
991,479 + ABZR87_RS20525 0.33 -0.4
991,479 + ABZR87_RS20525 0.33 -0.7
991,479 + ABZR87_RS20525 0.33 +2.1
991,526 - ABZR87_RS20525 0.42 -0.6
991,638 + ABZR87_RS20525 0.63 -0.2
991,638 + ABZR87_RS20525 0.63 +0.1
991,638 + ABZR87_RS20525 0.63 -0.2
991,638 + ABZR87_RS20525 0.63 -0.2
991,639 - ABZR87_RS20525 0.63 +0.1
991,639 - ABZR87_RS20525 0.63 +0.1
991,775 - ABZR87_RS20525 0.89 -0.0
991,775 - ABZR87_RS20525 0.89 -0.7
991,803 + -0.1
991,804 - +0.2
991,804 - -0.7
991,804 - -1.8
991,888 + +0.0
991,888 + -0.2
991,889 - -1.2
991,889 - +0.3
991,889 - +1.0
991,904 + +0.2
992,026 - -0.5
992,166 + ABZR87_RS20530 0.25 -0.5
992,272 + ABZR87_RS20530 0.40 -0.9
992,273 - ABZR87_RS20530 0.40 -1.0
992,318 + ABZR87_RS20530 0.46 -0.5
992,333 + ABZR87_RS20530 0.48 -1.0
992,333 + ABZR87_RS20530 0.48 -0.9
992,333 + ABZR87_RS20530 0.48 -1.4
992,333 + ABZR87_RS20530 0.48 +0.0
992,334 - ABZR87_RS20530 0.48 -0.1
992,488 + ABZR87_RS20530 0.69 -1.5
992,488 + ABZR87_RS20530 0.69 -0.8
992,488 + ABZR87_RS20530 0.69 +2.5
992,488 + ABZR87_RS20530 0.69 +0.4
992,489 - ABZR87_RS20530 0.70 -0.3
992,489 - ABZR87_RS20530 0.70 +0.7
992,489 - ABZR87_RS20530 0.70 -0.7
992,489 - ABZR87_RS20530 0.70 +3.7
992,489 - ABZR87_RS20530 0.70 +0.1
992,691 + +0.7
992,691 + -0.0
992,692 - -1.1
992,692 - -1.6
992,724 + -0.3
992,725 - +0.2
992,725 - +0.5
992,730 + -0.3
992,730 + +0.9
992,756 + -0.0
992,756 + +0.9
992,756 + +0.2
992,756 + +0.7
992,756 + +0.7
992,756 + +0.3
992,756 + -0.9
992,756 + +1.6
992,756 + +0.0
992,756 + +0.4
992,757 - +0.4
992,757 - -0.3
992,757 - -0.6
992,757 - -0.9
992,758 + -0.1
992,758 + +0.3
992,758 + -1.5
992,758 + +0.2
992,759 - +0.9
992,759 - -0.3
992,759 - +0.3
992,759 - -0.2

Or see this region's nucleotide sequence