Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS20500 and ABZR87_RS20505 are separated by 79 nucleotides ABZR87_RS20505 and ABZR87_RS20510 overlap by 8 nucleotides ABZR87_RS20510 and ABZR87_RS20515 are separated by 199 nucleotides ABZR87_RS20515 and ABZR87_RS20520 are separated by 32 nucleotides ABZR87_RS20520 and ABZR87_RS20525 are separated by 28 nucleotides
ABZR87_RS20500: ABZR87_RS20500 - hypothetical protein, at 989,291 to 989,533
_RS20500
ABZR87_RS20505: ABZR87_RS20505 - hypothetical protein, at 989,613 to 989,813
_RS20505
ABZR87_RS20510: ABZR87_RS20510 - LysR family transcriptional regulator, at 989,806 to 989,880
_RS20510
ABZR87_RS20515: ABZR87_RS20515 - ferredoxin FdxA, at 990,080 to 990,418
_RS20515
ABZR87_RS20520: ABZR87_RS20520 - crotonase/enoyl-CoA hydratase family protein, at 990,451 to 991,272
_RS20520
ABZR87_RS20525: ABZR87_RS20525 - Mpo1-like protein, at 991,301 to 991,834
_RS20525
Position (kb) 990
991 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 989.101 kb on + strand at 989.101 kb on + strand at 989.134 kb on + strand at 989.134 kb on + strand at 989.134 kb on + strand at 989.165 kb on + strand at 989.165 kb on + strand at 989.166 kb on - strand at 989.166 kb on - strand at 989.166 kb on - strand at 989.203 kb on + strand at 989.204 kb on - strand at 989.210 kb on + strand at 989.210 kb on + strand at 989.211 kb on - strand at 989.250 kb on - strand at 989.287 kb on - strand at 989.378 kb on + strand, within ABZR87_RS20500 at 989.531 kb on + strand at 989.531 kb on + strand at 989.532 kb on - strand at 989.541 kb on + strand at 989.541 kb on + strand at 989.541 kb on + strand at 989.541 kb on + strand at 989.545 kb on + strand at 989.545 kb on + strand at 989.545 kb on + strand at 989.546 kb on - strand at 989.546 kb on - strand at 989.546 kb on - strand at 989.552 kb on + strand at 989.552 kb on + strand at 989.552 kb on + strand at 989.552 kb on + strand at 989.552 kb on + strand at 989.609 kb on + strand at 989.609 kb on + strand at 989.610 kb on - strand at 989.610 kb on - strand at 989.746 kb on + strand, within ABZR87_RS20505 at 989.747 kb on - strand, within ABZR87_RS20505 at 989.747 kb on - strand, within ABZR87_RS20505 at 989.873 kb on - strand at 989.901 kb on + strand at 989.902 kb on - strand at 989.902 kb on - strand at 989.902 kb on - strand at 989.915 kb on - strand at 989.921 kb on + strand at 989.922 kb on - strand at 989.922 kb on - strand at 989.922 kb on - strand at 989.927 kb on + strand at 989.927 kb on + strand at 989.927 kb on + strand at 989.928 kb on - strand at 989.928 kb on - strand at 989.930 kb on - strand at 990.035 kb on - strand at 990.075 kb on + strand at 990.075 kb on + strand at 990.075 kb on + strand at 990.079 kb on + strand at 990.079 kb on + strand at 990.079 kb on + strand at 990.079 kb on + strand at 990.079 kb on + strand at 990.080 kb on - strand at 990.080 kb on - strand at 990.080 kb on - strand at 990.080 kb on - strand at 990.080 kb on - strand at 990.086 kb on + strand at 990.086 kb on + strand at 990.086 kb on + strand at 990.086 kb on + strand at 990.086 kb on + strand at 990.086 kb on + strand at 990.086 kb on + strand at 990.086 kb on + strand at 990.086 kb on + strand at 990.087 kb on - strand at 990.087 kb on - strand at 990.087 kb on - strand at 990.090 kb on + strand at 990.090 kb on + strand at 990.090 kb on + strand at 990.091 kb on - strand at 990.091 kb on - strand at 990.091 kb on - strand at 990.091 kb on - strand at 990.119 kb on + strand, within ABZR87_RS20515 at 990.120 kb on - strand, within ABZR87_RS20515 at 990.120 kb on - strand, within ABZR87_RS20515 at 990.245 kb on + strand, within ABZR87_RS20515 at 990.245 kb on + strand, within ABZR87_RS20515 at 990.645 kb on + strand, within ABZR87_RS20520 at 990.745 kb on + strand, within ABZR87_RS20520 at 990.746 kb on - strand, within ABZR87_RS20520 at 990.746 kb on - strand, within ABZR87_RS20520 at 990.746 kb on - strand, within ABZR87_RS20520 at 990.809 kb on - strand, within ABZR87_RS20520 at 990.850 kb on + strand, within ABZR87_RS20520 at 990.850 kb on + strand, within ABZR87_RS20520 at 990.851 kb on - strand, within ABZR87_RS20520 at 990.970 kb on + strand, within ABZR87_RS20520 at 990.970 kb on + strand, within ABZR87_RS20520 at 990.970 kb on + strand, within ABZR87_RS20520 at 991.271 kb on - strand at 991.283 kb on + strand at 991.283 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 989,101 + -0.1 989,101 + +2.4 989,134 + -0.1 989,134 + -0.4 989,134 + -0.2 989,165 + +0.8 989,165 + -0.5 989,166 - -0.1 989,166 - +0.2 989,166 - +0.2 989,203 + +0.2 989,204 - +0.1 989,210 + +0.7 989,210 + -0.4 989,211 - -0.5 989,250 - +0.6 989,287 - +0.3 989,378 + ABZR87_RS20500 0.36 -0.6 989,531 + +1.1 989,531 + +0.4 989,532 - +0.1 989,541 + +0.3 989,541 + -0.1 989,541 + +0.2 989,541 + +0.7 989,545 + -0.8 989,545 + +1.0 989,545 + +0.3 989,546 - +0.3 989,546 - +1.5 989,546 - -0.7 989,552 + +0.6 989,552 + +0.3 989,552 + -0.1 989,552 + -1.1 989,552 + +1.6 989,609 + -1.8 989,609 + -0.4 989,610 - +0.1 989,610 - -1.0 989,746 + ABZR87_RS20505 0.66 +0.1 989,747 - ABZR87_RS20505 0.67 +0.3 989,747 - ABZR87_RS20505 0.67 -1.2 989,873 - +0.6 989,901 + +0.3 989,902 - -0.4 989,902 - +0.3 989,902 - +2.9 989,915 - -1.3 989,921 + -0.5 989,922 - +0.2 989,922 - -0.2 989,922 - -1.2 989,927 + -0.5 989,927 + +0.3 989,927 + -0.1 989,928 - -0.1 989,928 - -0.3 989,930 - +0.2 990,035 - -1.2 990,075 + +0.3 990,075 + -1.0 990,075 + +0.2 990,079 + -0.7 990,079 + +0.3 990,079 + +0.4 990,079 + +0.7 990,079 + -0.7 990,080 - +0.2 990,080 - -0.3 990,080 - +0.1 990,080 - -0.1 990,080 - +0.2 990,086 + +0.7 990,086 + -0.2 990,086 + -1.0 990,086 + -2.4 990,086 + -0.5 990,086 + -0.2 990,086 + -0.6 990,086 + +0.4 990,086 + -0.4 990,087 - -0.5 990,087 - -1.1 990,087 - +1.6 990,090 + -1.4 990,090 + -0.8 990,090 + -1.0 990,091 - +0.3 990,091 - +1.1 990,091 - -0.5 990,091 - +1.0 990,119 + ABZR87_RS20515 0.12 +0.8 990,120 - ABZR87_RS20515 0.12 -0.7 990,120 - ABZR87_RS20515 0.12 -0.7 990,245 + ABZR87_RS20515 0.49 -0.7 990,245 + ABZR87_RS20515 0.49 +0.2 990,645 + ABZR87_RS20520 0.24 +0.1 990,745 + ABZR87_RS20520 0.36 -1.0 990,746 - ABZR87_RS20520 0.36 -0.4 990,746 - ABZR87_RS20520 0.36 +0.5 990,746 - ABZR87_RS20520 0.36 +0.2 990,809 - ABZR87_RS20520 0.44 +0.3 990,850 + ABZR87_RS20520 0.49 +0.1 990,850 + ABZR87_RS20520 0.49 +0.3 990,851 - ABZR87_RS20520 0.49 +0.3 990,970 + ABZR87_RS20520 0.63 +0.6 990,970 + ABZR87_RS20520 0.63 +0.9 990,970 + ABZR87_RS20520 0.63 -0.2 991,271 - +0.5 991,283 + +0.7 991,283 + -0.1
Or see this region's nucleotide sequence