Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS19525 and ABZR87_RS19530 are separated by 76 nucleotides ABZR87_RS19530 and ABZR87_RS19535 overlap by 8 nucleotides ABZR87_RS19535 and ABZR87_RS19540 are separated by 9 nucleotides
ABZR87_RS19525: ABZR87_RS19525 - TadE/TadG family type IV pilus assembly protein, at 752,971 to 753,474
_RS19525
ABZR87_RS19530: ABZR87_RS19530 - Flp pilus assembly protein CpaB, at 753,551 to 754,477
_RS19530
ABZR87_RS19535: ABZR87_RS19535 - type II and III secretion system protein family protein, at 754,470 to 755,834
_RS19535
ABZR87_RS19540: ABZR87_RS19540 - CpaE family protein, at 755,844 to 757,187
_RS19540
Position (kb)
754
755
756 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 753.472 kb on + strand at 753.472 kb on + strand at 753.472 kb on + strand at 753.472 kb on + strand at 753.473 kb on - strand at 753.473 kb on - strand at 753.473 kb on - strand at 753.536 kb on - strand at 753.619 kb on - strand at 753.681 kb on + strand, within ABZR87_RS19530 at 753.681 kb on + strand, within ABZR87_RS19530 at 753.681 kb on + strand, within ABZR87_RS19530 at 753.681 kb on + strand, within ABZR87_RS19530 at 753.681 kb on + strand, within ABZR87_RS19530 at 753.681 kb on + strand, within ABZR87_RS19530 at 753.681 kb on + strand, within ABZR87_RS19530 at 753.682 kb on - strand, within ABZR87_RS19530 at 753.682 kb on - strand, within ABZR87_RS19530 at 753.682 kb on - strand, within ABZR87_RS19530 at 753.682 kb on - strand, within ABZR87_RS19530 at 753.682 kb on - strand, within ABZR87_RS19530 at 753.682 kb on - strand, within ABZR87_RS19530 at 753.682 kb on - strand, within ABZR87_RS19530 at 753.683 kb on + strand, within ABZR87_RS19530 at 753.698 kb on - strand, within ABZR87_RS19530 at 753.698 kb on - strand, within ABZR87_RS19530 at 753.962 kb on + strand, within ABZR87_RS19530 at 753.962 kb on + strand, within ABZR87_RS19530 at 753.963 kb on - strand, within ABZR87_RS19530 at 753.963 kb on - strand, within ABZR87_RS19530 at 754.507 kb on + strand at 754.507 kb on + strand at 754.770 kb on + strand, within ABZR87_RS19535 at 754.770 kb on + strand, within ABZR87_RS19535 at 754.770 kb on + strand, within ABZR87_RS19535 at 754.770 kb on + strand, within ABZR87_RS19535 at 754.770 kb on + strand, within ABZR87_RS19535 at 754.770 kb on + strand, within ABZR87_RS19535 at 754.770 kb on + strand, within ABZR87_RS19535 at 754.770 kb on + strand, within ABZR87_RS19535 at 754.771 kb on - strand, within ABZR87_RS19535 at 754.771 kb on - strand, within ABZR87_RS19535 at 754.771 kb on - strand, within ABZR87_RS19535 at 754.771 kb on - strand, within ABZR87_RS19535 at 754.867 kb on + strand, within ABZR87_RS19535 at 754.867 kb on + strand, within ABZR87_RS19535 at 754.867 kb on + strand, within ABZR87_RS19535 at 754.867 kb on + strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.869 kb on + strand, within ABZR87_RS19535 at 755.343 kb on + strand, within ABZR87_RS19535 at 755.343 kb on + strand, within ABZR87_RS19535 at 755.344 kb on - strand, within ABZR87_RS19535 at 755.344 kb on - strand, within ABZR87_RS19535 at 755.344 kb on - strand, within ABZR87_RS19535 at 755.344 kb on - strand, within ABZR87_RS19535 at 755.501 kb on + strand, within ABZR87_RS19535 at 755.567 kb on - strand, within ABZR87_RS19535 at 755.637 kb on + strand, within ABZR87_RS19535 at 755.637 kb on + strand, within ABZR87_RS19535 at 755.638 kb on - strand, within ABZR87_RS19535 at 755.638 kb on - strand, within ABZR87_RS19535 at 755.638 kb on - strand, within ABZR87_RS19535 at 755.638 kb on - strand, within ABZR87_RS19535 at 755.638 kb on - strand, within ABZR87_RS19535 at 755.672 kb on + strand, within ABZR87_RS19535 at 755.673 kb on - strand, within ABZR87_RS19535 at 755.832 kb on + strand at 755.832 kb on + strand at 755.833 kb on - strand at 755.833 kb on - strand at 755.833 kb on - strand at 755.850 kb on + strand at 755.850 kb on + strand at 755.850 kb on + strand at 755.850 kb on + strand at 755.851 kb on - strand at 755.851 kb on - strand at 755.851 kb on - strand at 755.851 kb on - strand at 756.058 kb on + strand, within ABZR87_RS19540 at 756.058 kb on + strand, within ABZR87_RS19540 at 756.058 kb on + strand, within ABZR87_RS19540 at 756.460 kb on - strand, within ABZR87_RS19540 at 756.460 kb on - strand, within ABZR87_RS19540
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 753,472 + -0.3 753,472 + -0.6 753,472 + +0.1 753,472 + +0.5 753,473 - -1.3 753,473 - +0.0 753,473 - -0.7 753,536 - +0.4 753,619 - +0.9 753,681 + ABZR87_RS19530 0.14 -1.0 753,681 + ABZR87_RS19530 0.14 +0.7 753,681 + ABZR87_RS19530 0.14 -1.2 753,681 + ABZR87_RS19530 0.14 -0.4 753,681 + ABZR87_RS19530 0.14 -0.5 753,681 + ABZR87_RS19530 0.14 +0.5 753,681 + ABZR87_RS19530 0.14 -0.1 753,682 - ABZR87_RS19530 0.14 -0.5 753,682 - ABZR87_RS19530 0.14 -0.1 753,682 - ABZR87_RS19530 0.14 -0.8 753,682 - ABZR87_RS19530 0.14 -1.3 753,682 - ABZR87_RS19530 0.14 +1.3 753,682 - ABZR87_RS19530 0.14 -2.0 753,682 - ABZR87_RS19530 0.14 -0.6 753,683 + ABZR87_RS19530 0.14 -1.2 753,698 - ABZR87_RS19530 0.16 -3.0 753,698 - ABZR87_RS19530 0.16 -0.8 753,962 + ABZR87_RS19530 0.44 -0.7 753,962 + ABZR87_RS19530 0.44 -1.8 753,963 - ABZR87_RS19530 0.44 -1.1 753,963 - ABZR87_RS19530 0.44 +0.7 754,507 + -1.1 754,507 + -0.6 754,770 + ABZR87_RS19535 0.22 -0.9 754,770 + ABZR87_RS19535 0.22 +0.1 754,770 + ABZR87_RS19535 0.22 -1.5 754,770 + ABZR87_RS19535 0.22 -1.2 754,770 + ABZR87_RS19535 0.22 -0.9 754,770 + ABZR87_RS19535 0.22 -1.0 754,770 + ABZR87_RS19535 0.22 -1.2 754,770 + ABZR87_RS19535 0.22 -1.1 754,771 - ABZR87_RS19535 0.22 -1.2 754,771 - ABZR87_RS19535 0.22 -0.7 754,771 - ABZR87_RS19535 0.22 +0.1 754,771 - ABZR87_RS19535 0.22 -0.6 754,867 + ABZR87_RS19535 0.29 -0.6 754,867 + ABZR87_RS19535 0.29 -0.5 754,867 + ABZR87_RS19535 0.29 -1.2 754,867 + ABZR87_RS19535 0.29 -0.2 754,868 - ABZR87_RS19535 0.29 -1.1 754,868 - ABZR87_RS19535 0.29 -1.4 754,868 - ABZR87_RS19535 0.29 -0.6 754,868 - ABZR87_RS19535 0.29 -1.8 754,868 - ABZR87_RS19535 0.29 +1.2 754,868 - ABZR87_RS19535 0.29 +0.1 754,868 - ABZR87_RS19535 0.29 -1.0 754,868 - ABZR87_RS19535 0.29 -0.7 754,868 - ABZR87_RS19535 0.29 +0.1 754,868 - ABZR87_RS19535 0.29 -0.9 754,868 - ABZR87_RS19535 0.29 -1.6 754,868 - ABZR87_RS19535 0.29 +0.8 754,869 + ABZR87_RS19535 0.29 -0.2 755,343 + ABZR87_RS19535 0.64 -1.2 755,343 + ABZR87_RS19535 0.64 -0.8 755,344 - ABZR87_RS19535 0.64 +0.4 755,344 - ABZR87_RS19535 0.64 -1.5 755,344 - ABZR87_RS19535 0.64 -0.1 755,344 - ABZR87_RS19535 0.64 -0.2 755,501 + ABZR87_RS19535 0.76 -1.6 755,567 - ABZR87_RS19535 0.80 +0.4 755,637 + ABZR87_RS19535 0.85 -0.3 755,637 + ABZR87_RS19535 0.85 -0.8 755,638 - ABZR87_RS19535 0.86 -0.3 755,638 - ABZR87_RS19535 0.86 -0.1 755,638 - ABZR87_RS19535 0.86 -0.8 755,638 - ABZR87_RS19535 0.86 -0.8 755,638 - ABZR87_RS19535 0.86 -0.5 755,672 + ABZR87_RS19535 0.88 -0.0 755,673 - ABZR87_RS19535 0.88 -0.7 755,832 + +0.2 755,832 + +0.0 755,833 - -0.6 755,833 - +0.2 755,833 - -0.5 755,850 + +0.4 755,850 + +0.5 755,850 + +2.3 755,850 + -0.5 755,851 - -1.4 755,851 - +1.5 755,851 - +0.3 755,851 - -0.5 756,058 + ABZR87_RS19540 0.16 +0.3 756,058 + ABZR87_RS19540 0.16 -0.3 756,058 + ABZR87_RS19540 0.16 -1.3 756,460 - ABZR87_RS19540 0.46 +0.0 756,460 - ABZR87_RS19540 0.46 -0.3
Or see this region's nucleotide sequence