Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS19520 and ABZR87_RS19525 are separated by 62 nucleotides ABZR87_RS19525 and ABZR87_RS19530 are separated by 76 nucleotides ABZR87_RS19530 and ABZR87_RS19535 overlap by 8 nucleotides
ABZR87_RS19520: ABZR87_RS19520 - prepilin peptidase, at 752,444 to 752,908
_RS19520
ABZR87_RS19525: ABZR87_RS19525 - TadE/TadG family type IV pilus assembly protein, at 752,971 to 753,474
_RS19525
ABZR87_RS19530: ABZR87_RS19530 - Flp pilus assembly protein CpaB, at 753,551 to 754,477
_RS19530
ABZR87_RS19535: ABZR87_RS19535 - type II and III secretion system protein family protein, at 754,470 to 755,834
_RS19535
Position (kb) 753
754
755 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 752.582 kb on + strand, within ABZR87_RS19520 at 752.582 kb on + strand, within ABZR87_RS19520 at 752.582 kb on + strand, within ABZR87_RS19520 at 752.582 kb on + strand, within ABZR87_RS19520 at 752.582 kb on + strand, within ABZR87_RS19520 at 752.582 kb on + strand, within ABZR87_RS19520 at 752.583 kb on - strand, within ABZR87_RS19520 at 752.583 kb on - strand, within ABZR87_RS19520 at 752.583 kb on - strand, within ABZR87_RS19520 at 752.583 kb on - strand, within ABZR87_RS19520 at 752.583 kb on - strand, within ABZR87_RS19520 at 752.583 kb on - strand, within ABZR87_RS19520 at 752.583 kb on - strand, within ABZR87_RS19520 at 752.687 kb on - strand, within ABZR87_RS19520 at 752.687 kb on - strand, within ABZR87_RS19520 at 752.687 kb on - strand, within ABZR87_RS19520 at 752.819 kb on - strand, within ABZR87_RS19520 at 752.819 kb on - strand, within ABZR87_RS19520 at 752.834 kb on + strand, within ABZR87_RS19520 at 752.834 kb on + strand, within ABZR87_RS19520 at 752.834 kb on + strand, within ABZR87_RS19520 at 752.834 kb on + strand, within ABZR87_RS19520 at 752.834 kb on + strand, within ABZR87_RS19520 at 752.834 kb on + strand, within ABZR87_RS19520 at 752.834 kb on + strand, within ABZR87_RS19520 at 752.834 kb on + strand, within ABZR87_RS19520 at 752.834 kb on + strand, within ABZR87_RS19520 at 752.835 kb on - strand, within ABZR87_RS19520 at 752.835 kb on - strand, within ABZR87_RS19520 at 752.835 kb on - strand, within ABZR87_RS19520 at 752.835 kb on - strand, within ABZR87_RS19520 at 752.835 kb on - strand, within ABZR87_RS19520 at 752.835 kb on - strand, within ABZR87_RS19520 at 752.835 kb on - strand, within ABZR87_RS19520 at 752.835 kb on - strand, within ABZR87_RS19520 at 752.835 kb on - strand, within ABZR87_RS19520 at 752.835 kb on - strand, within ABZR87_RS19520 at 752.835 kb on - strand, within ABZR87_RS19520 at 752.835 kb on - strand, within ABZR87_RS19520 at 752.835 kb on - strand, within ABZR87_RS19520 at 752.835 kb on - strand, within ABZR87_RS19520 at 752.836 kb on + strand, within ABZR87_RS19520 at 752.836 kb on + strand, within ABZR87_RS19520 at 752.837 kb on - strand, within ABZR87_RS19520 at 752.907 kb on - strand at 752.907 kb on - strand at 752.907 kb on - strand at 752.907 kb on - strand at 752.953 kb on + strand at 752.953 kb on + strand at 752.954 kb on - strand at 752.955 kb on + strand at 752.956 kb on - strand at 752.956 kb on - strand at 752.956 kb on - strand at 752.956 kb on - strand at 753.004 kb on + strand at 753.004 kb on + strand at 753.004 kb on + strand at 753.004 kb on + strand at 753.004 kb on + strand at 753.004 kb on + strand at 753.004 kb on + strand at 753.004 kb on + strand at 753.004 kb on + strand at 753.004 kb on + strand at 753.005 kb on - strand at 753.005 kb on - strand at 753.005 kb on - strand at 753.005 kb on - strand at 753.005 kb on - strand at 753.005 kb on - strand at 753.005 kb on - strand at 753.005 kb on - strand at 753.005 kb on - strand at 753.005 kb on - strand at 753.005 kb on - strand at 753.005 kb on - strand at 753.005 kb on - strand at 753.019 kb on - strand at 753.032 kb on + strand, within ABZR87_RS19525 at 753.130 kb on + strand, within ABZR87_RS19525 at 753.131 kb on - strand, within ABZR87_RS19525 at 753.131 kb on - strand, within ABZR87_RS19525 at 753.131 kb on - strand, within ABZR87_RS19525 at 753.131 kb on - strand, within ABZR87_RS19525 at 753.131 kb on - strand, within ABZR87_RS19525 at 753.131 kb on - strand, within ABZR87_RS19525 at 753.131 kb on - strand, within ABZR87_RS19525 at 753.133 kb on + strand, within ABZR87_RS19525 at 753.134 kb on - strand, within ABZR87_RS19525 at 753.134 kb on - strand, within ABZR87_RS19525 at 753.134 kb on - strand, within ABZR87_RS19525 at 753.134 kb on - strand, within ABZR87_RS19525 at 753.206 kb on - strand, within ABZR87_RS19525 at 753.206 kb on - strand, within ABZR87_RS19525 at 753.206 kb on - strand, within ABZR87_RS19525 at 753.242 kb on - strand, within ABZR87_RS19525 at 753.242 kb on - strand, within ABZR87_RS19525 at 753.261 kb on - strand, within ABZR87_RS19525 at 753.407 kb on + strand, within ABZR87_RS19525 at 753.408 kb on - strand, within ABZR87_RS19525 at 753.408 kb on - strand, within ABZR87_RS19525 at 753.472 kb on + strand at 753.472 kb on + strand at 753.472 kb on + strand at 753.472 kb on + strand at 753.473 kb on - strand at 753.473 kb on - strand at 753.473 kb on - strand at 753.536 kb on - strand at 753.619 kb on - strand at 753.681 kb on + strand, within ABZR87_RS19530 at 753.681 kb on + strand, within ABZR87_RS19530 at 753.681 kb on + strand, within ABZR87_RS19530 at 753.681 kb on + strand, within ABZR87_RS19530 at 753.681 kb on + strand, within ABZR87_RS19530 at 753.681 kb on + strand, within ABZR87_RS19530 at 753.681 kb on + strand, within ABZR87_RS19530 at 753.682 kb on - strand, within ABZR87_RS19530 at 753.682 kb on - strand, within ABZR87_RS19530 at 753.682 kb on - strand, within ABZR87_RS19530 at 753.682 kb on - strand, within ABZR87_RS19530 at 753.682 kb on - strand, within ABZR87_RS19530 at 753.682 kb on - strand, within ABZR87_RS19530 at 753.682 kb on - strand, within ABZR87_RS19530 at 753.683 kb on + strand, within ABZR87_RS19530 at 753.698 kb on - strand, within ABZR87_RS19530 at 753.698 kb on - strand, within ABZR87_RS19530 at 753.962 kb on + strand, within ABZR87_RS19530 at 753.962 kb on + strand, within ABZR87_RS19530 at 753.963 kb on - strand, within ABZR87_RS19530 at 753.963 kb on - strand, within ABZR87_RS19530 at 754.507 kb on + strand at 754.507 kb on + strand at 754.770 kb on + strand, within ABZR87_RS19535 at 754.770 kb on + strand, within ABZR87_RS19535 at 754.770 kb on + strand, within ABZR87_RS19535 at 754.770 kb on + strand, within ABZR87_RS19535 at 754.770 kb on + strand, within ABZR87_RS19535 at 754.770 kb on + strand, within ABZR87_RS19535 at 754.770 kb on + strand, within ABZR87_RS19535 at 754.770 kb on + strand, within ABZR87_RS19535 at 754.771 kb on - strand, within ABZR87_RS19535 at 754.771 kb on - strand, within ABZR87_RS19535 at 754.771 kb on - strand, within ABZR87_RS19535 at 754.771 kb on - strand, within ABZR87_RS19535 at 754.867 kb on + strand, within ABZR87_RS19535 at 754.867 kb on + strand, within ABZR87_RS19535 at 754.867 kb on + strand, within ABZR87_RS19535 at 754.867 kb on + strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.869 kb on + strand, within ABZR87_RS19535 at 755.343 kb on + strand, within ABZR87_RS19535 at 755.343 kb on + strand, within ABZR87_RS19535 at 755.344 kb on - strand, within ABZR87_RS19535 at 755.344 kb on - strand, within ABZR87_RS19535 at 755.344 kb on - strand, within ABZR87_RS19535 at 755.344 kb on - strand, within ABZR87_RS19535
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 752,582 + ABZR87_RS19520 0.30 -0.4 752,582 + ABZR87_RS19520 0.30 +0.4 752,582 + ABZR87_RS19520 0.30 -1.9 752,582 + ABZR87_RS19520 0.30 -1.1 752,582 + ABZR87_RS19520 0.30 -1.1 752,582 + ABZR87_RS19520 0.30 +0.3 752,583 - ABZR87_RS19520 0.30 -1.1 752,583 - ABZR87_RS19520 0.30 -0.0 752,583 - ABZR87_RS19520 0.30 -0.6 752,583 - ABZR87_RS19520 0.30 -0.4 752,583 - ABZR87_RS19520 0.30 -1.7 752,583 - ABZR87_RS19520 0.30 -1.8 752,583 - ABZR87_RS19520 0.30 +0.6 752,687 - ABZR87_RS19520 0.52 +0.3 752,687 - ABZR87_RS19520 0.52 -1.2 752,687 - ABZR87_RS19520 0.52 +0.5 752,819 - ABZR87_RS19520 0.81 -0.2 752,819 - ABZR87_RS19520 0.81 -1.2 752,834 + ABZR87_RS19520 0.84 -0.1 752,834 + ABZR87_RS19520 0.84 +0.1 752,834 + ABZR87_RS19520 0.84 -1.0 752,834 + ABZR87_RS19520 0.84 -3.6 752,834 + ABZR87_RS19520 0.84 -1.0 752,834 + ABZR87_RS19520 0.84 -1.1 752,834 + ABZR87_RS19520 0.84 -1.9 752,834 + ABZR87_RS19520 0.84 -1.0 752,834 + ABZR87_RS19520 0.84 -0.1 752,835 - ABZR87_RS19520 0.84 -1.6 752,835 - ABZR87_RS19520 0.84 -0.6 752,835 - ABZR87_RS19520 0.84 -0.2 752,835 - ABZR87_RS19520 0.84 +0.5 752,835 - ABZR87_RS19520 0.84 +0.2 752,835 - ABZR87_RS19520 0.84 -0.1 752,835 - ABZR87_RS19520 0.84 -0.3 752,835 - ABZR87_RS19520 0.84 -0.7 752,835 - ABZR87_RS19520 0.84 -0.6 752,835 - ABZR87_RS19520 0.84 +0.5 752,835 - ABZR87_RS19520 0.84 -0.6 752,835 - ABZR87_RS19520 0.84 -0.9 752,835 - ABZR87_RS19520 0.84 -0.5 752,835 - ABZR87_RS19520 0.84 +0.2 752,836 + ABZR87_RS19520 0.84 -0.7 752,836 + ABZR87_RS19520 0.84 -0.8 752,837 - ABZR87_RS19520 0.85 -0.5 752,907 - -0.6 752,907 - +0.0 752,907 - -0.8 752,907 - +0.8 752,953 + -0.2 752,953 + -0.2 752,954 - +2.7 752,955 + -0.7 752,956 - +0.1 752,956 - -1.3 752,956 - -0.8 752,956 - -0.8 753,004 + -0.7 753,004 + +0.2 753,004 + -1.0 753,004 + -0.2 753,004 + -1.2 753,004 + +1.2 753,004 + -0.7 753,004 + -0.7 753,004 + -0.2 753,004 + +0.8 753,005 - -1.2 753,005 - +0.5 753,005 - -0.6 753,005 - +0.7 753,005 - -0.0 753,005 - -0.4 753,005 - -0.2 753,005 - +0.9 753,005 - -0.4 753,005 - -0.1 753,005 - -0.8 753,005 - -0.0 753,005 - +0.2 753,019 - -0.9 753,032 + ABZR87_RS19525 0.12 -1.6 753,130 + ABZR87_RS19525 0.32 -1.1 753,131 - ABZR87_RS19525 0.32 -0.6 753,131 - ABZR87_RS19525 0.32 -0.3 753,131 - ABZR87_RS19525 0.32 -0.1 753,131 - ABZR87_RS19525 0.32 -2.5 753,131 - ABZR87_RS19525 0.32 +0.6 753,131 - ABZR87_RS19525 0.32 -0.8 753,131 - ABZR87_RS19525 0.32 +0.3 753,133 + ABZR87_RS19525 0.32 -1.4 753,134 - ABZR87_RS19525 0.32 -0.3 753,134 - ABZR87_RS19525 0.32 -0.7 753,134 - ABZR87_RS19525 0.32 -0.7 753,134 - ABZR87_RS19525 0.32 +1.2 753,206 - ABZR87_RS19525 0.47 -0.9 753,206 - ABZR87_RS19525 0.47 +0.9 753,206 - ABZR87_RS19525 0.47 -0.3 753,242 - ABZR87_RS19525 0.54 +0.5 753,242 - ABZR87_RS19525 0.54 +0.1 753,261 - ABZR87_RS19525 0.58 -0.6 753,407 + ABZR87_RS19525 0.87 -0.9 753,408 - ABZR87_RS19525 0.87 -1.6 753,408 - ABZR87_RS19525 0.87 -0.2 753,472 + -0.3 753,472 + -0.6 753,472 + +0.1 753,472 + +0.5 753,473 - -1.3 753,473 - +0.0 753,473 - -0.7 753,536 - +0.4 753,619 - +0.9 753,681 + ABZR87_RS19530 0.14 -1.0 753,681 + ABZR87_RS19530 0.14 +0.7 753,681 + ABZR87_RS19530 0.14 -1.2 753,681 + ABZR87_RS19530 0.14 -0.4 753,681 + ABZR87_RS19530 0.14 -0.5 753,681 + ABZR87_RS19530 0.14 +0.5 753,681 + ABZR87_RS19530 0.14 -0.1 753,682 - ABZR87_RS19530 0.14 -0.5 753,682 - ABZR87_RS19530 0.14 -0.1 753,682 - ABZR87_RS19530 0.14 -0.8 753,682 - ABZR87_RS19530 0.14 -1.3 753,682 - ABZR87_RS19530 0.14 +1.3 753,682 - ABZR87_RS19530 0.14 -2.0 753,682 - ABZR87_RS19530 0.14 -0.6 753,683 + ABZR87_RS19530 0.14 -1.2 753,698 - ABZR87_RS19530 0.16 -3.0 753,698 - ABZR87_RS19530 0.16 -0.8 753,962 + ABZR87_RS19530 0.44 -0.7 753,962 + ABZR87_RS19530 0.44 -1.8 753,963 - ABZR87_RS19530 0.44 -1.1 753,963 - ABZR87_RS19530 0.44 +0.7 754,507 + -1.1 754,507 + -0.6 754,770 + ABZR87_RS19535 0.22 -0.9 754,770 + ABZR87_RS19535 0.22 +0.1 754,770 + ABZR87_RS19535 0.22 -1.5 754,770 + ABZR87_RS19535 0.22 -1.2 754,770 + ABZR87_RS19535 0.22 -0.9 754,770 + ABZR87_RS19535 0.22 -1.0 754,770 + ABZR87_RS19535 0.22 -1.2 754,770 + ABZR87_RS19535 0.22 -1.1 754,771 - ABZR87_RS19535 0.22 -1.2 754,771 - ABZR87_RS19535 0.22 -0.7 754,771 - ABZR87_RS19535 0.22 +0.1 754,771 - ABZR87_RS19535 0.22 -0.6 754,867 + ABZR87_RS19535 0.29 -0.6 754,867 + ABZR87_RS19535 0.29 -0.5 754,867 + ABZR87_RS19535 0.29 -1.2 754,867 + ABZR87_RS19535 0.29 -0.2 754,868 - ABZR87_RS19535 0.29 -1.1 754,868 - ABZR87_RS19535 0.29 -1.4 754,868 - ABZR87_RS19535 0.29 -0.6 754,868 - ABZR87_RS19535 0.29 -1.8 754,868 - ABZR87_RS19535 0.29 +1.2 754,868 - ABZR87_RS19535 0.29 +0.1 754,868 - ABZR87_RS19535 0.29 -1.0 754,868 - ABZR87_RS19535 0.29 -0.7 754,868 - ABZR87_RS19535 0.29 +0.1 754,868 - ABZR87_RS19535 0.29 -0.9 754,868 - ABZR87_RS19535 0.29 -1.6 754,868 - ABZR87_RS19535 0.29 +0.8 754,869 + ABZR87_RS19535 0.29 -0.2 755,343 + ABZR87_RS19535 0.64 -1.2 755,343 + ABZR87_RS19535 0.64 -0.8 755,344 - ABZR87_RS19535 0.64 +0.4 755,344 - ABZR87_RS19535 0.64 -1.5 755,344 - ABZR87_RS19535 0.64 -0.1 755,344 - ABZR87_RS19535 0.64 -0.2
Or see this region's nucleotide sequence