Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS18825 and ABZR87_RS18830 are separated by 7 nucleotides ABZR87_RS18830 and ABZR87_RS18835 are separated by 86 nucleotides ABZR87_RS18835 and ABZR87_RS18840 overlap by 4 nucleotides
ABZR87_RS18825: ABZR87_RS18825 - LuxR family transcriptional regulator, at 576,904 to 578,022
_RS18825
ABZR87_RS18830: ABZR87_RS18830 - NIPSNAP family protein, at 578,030 to 578,335
_RS18830
ABZR87_RS18835: ABZR87_RS18835 - cytochrome d ubiquinol oxidase subunit II, at 578,422 to 579,453
_RS18835
ABZR87_RS18840: ABZR87_RS18840 - cytochrome ubiquinol oxidase subunit I, at 579,450 to 580,838
_RS18840
Position (kb) 578
579
580 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 577.928 kb on + strand at 577.928 kb on + strand at 577.928 kb on + strand at 578.039 kb on + strand at 578.040 kb on - strand at 578.040 kb on - strand at 578.065 kb on - strand, within ABZR87_RS18830 at 578.094 kb on + strand, within ABZR87_RS18830 at 578.142 kb on + strand, within ABZR87_RS18830 at 578.142 kb on + strand, within ABZR87_RS18830 at 578.151 kb on + strand, within ABZR87_RS18830 at 578.220 kb on + strand, within ABZR87_RS18830 at 578.220 kb on + strand, within ABZR87_RS18830 at 578.221 kb on - strand, within ABZR87_RS18830 at 578.275 kb on - strand, within ABZR87_RS18830 at 578.275 kb on - strand, within ABZR87_RS18830 at 578.313 kb on + strand at 578.313 kb on + strand at 578.314 kb on - strand at 578.314 kb on - strand at 578.369 kb on + strand at 578.370 kb on - strand at 578.370 kb on - strand at 578.421 kb on - strand at 578.426 kb on + strand at 578.426 kb on + strand at 578.427 kb on - strand at 578.427 kb on - strand at 578.427 kb on - strand at 578.427 kb on - strand at 578.427 kb on - strand at 578.429 kb on + strand at 578.465 kb on + strand at 578.466 kb on - strand at 578.466 kb on - strand at 578.471 kb on + strand at 578.471 kb on + strand at 578.472 kb on - strand at 578.472 kb on - strand at 578.472 kb on - strand at 578.481 kb on - strand at 578.600 kb on + strand, within ABZR87_RS18835 at 579.020 kb on + strand, within ABZR87_RS18835 at 579.020 kb on + strand, within ABZR87_RS18835 at 579.020 kb on + strand, within ABZR87_RS18835 at 579.020 kb on + strand, within ABZR87_RS18835 at 579.020 kb on + strand, within ABZR87_RS18835 at 579.020 kb on + strand, within ABZR87_RS18835 at 579.020 kb on + strand, within ABZR87_RS18835 at 579.020 kb on + strand, within ABZR87_RS18835 at 579.020 kb on + strand, within ABZR87_RS18835 at 579.021 kb on - strand, within ABZR87_RS18835 at 579.021 kb on - strand, within ABZR87_RS18835 at 579.021 kb on - strand, within ABZR87_RS18835 at 579.021 kb on - strand, within ABZR87_RS18835 at 579.021 kb on - strand, within ABZR87_RS18835 at 579.021 kb on - strand, within ABZR87_RS18835 at 579.021 kb on - strand, within ABZR87_RS18835 at 579.021 kb on - strand, within ABZR87_RS18835 at 579.021 kb on - strand, within ABZR87_RS18835 at 579.022 kb on + strand, within ABZR87_RS18835 at 579.023 kb on - strand, within ABZR87_RS18835 at 579.023 kb on - strand, within ABZR87_RS18835 at 579.071 kb on + strand, within ABZR87_RS18835 at 579.071 kb on + strand, within ABZR87_RS18835 at 579.071 kb on + strand, within ABZR87_RS18835 at 579.072 kb on - strand, within ABZR87_RS18835 at 579.072 kb on - strand, within ABZR87_RS18835 at 579.072 kb on - strand, within ABZR87_RS18835 at 579.072 kb on - strand, within ABZR87_RS18835 at 579.072 kb on - strand, within ABZR87_RS18835 at 579.179 kb on + strand, within ABZR87_RS18835 at 579.180 kb on - strand, within ABZR87_RS18835 at 579.180 kb on - strand, within ABZR87_RS18835 at 579.180 kb on - strand, within ABZR87_RS18835 at 579.350 kb on + strand at 579.368 kb on + strand at 579.368 kb on + strand at 579.368 kb on + strand at 579.368 kb on + strand at 579.368 kb on + strand at 579.369 kb on - strand at 579.565 kb on + strand at 579.565 kb on + strand at 579.565 kb on + strand at 579.566 kb on - strand at 579.566 kb on - strand at 579.583 kb on + strand at 579.583 kb on + strand at 579.604 kb on + strand, within ABZR87_RS18840 at 579.604 kb on + strand, within ABZR87_RS18840 at 579.604 kb on + strand, within ABZR87_RS18840 at 579.612 kb on + strand, within ABZR87_RS18840 at 579.612 kb on + strand, within ABZR87_RS18840 at 579.612 kb on + strand, within ABZR87_RS18840 at 579.612 kb on + strand, within ABZR87_RS18840 at 579.613 kb on - strand, within ABZR87_RS18840 at 579.616 kb on + strand, within ABZR87_RS18840 at 579.617 kb on - strand, within ABZR87_RS18840 at 579.688 kb on + strand, within ABZR87_RS18840 at 579.689 kb on - strand, within ABZR87_RS18840 at 579.689 kb on - strand, within ABZR87_RS18840 at 579.724 kb on + strand, within ABZR87_RS18840 at 579.724 kb on + strand, within ABZR87_RS18840 at 579.724 kb on + strand, within ABZR87_RS18840 at 579.724 kb on + strand, within ABZR87_RS18840 at 579.724 kb on + strand, within ABZR87_RS18840 at 579.725 kb on - strand, within ABZR87_RS18840 at 579.725 kb on - strand, within ABZR87_RS18840 at 579.725 kb on - strand, within ABZR87_RS18840 at 579.725 kb on - strand, within ABZR87_RS18840 at 579.961 kb on + strand, within ABZR87_RS18840 at 579.961 kb on + strand, within ABZR87_RS18840 at 580.229 kb on - strand, within ABZR87_RS18840 at 580.367 kb on - strand, within ABZR87_RS18840
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 577,928 + +0.5 577,928 + -0.7 577,928 + -1.4 578,039 + +0.6 578,040 - -0.2 578,040 - -1.3 578,065 - ABZR87_RS18830 0.11 +1.5 578,094 + ABZR87_RS18830 0.21 -0.9 578,142 + ABZR87_RS18830 0.37 -0.3 578,142 + ABZR87_RS18830 0.37 +0.5 578,151 + ABZR87_RS18830 0.40 +0.6 578,220 + ABZR87_RS18830 0.62 +0.5 578,220 + ABZR87_RS18830 0.62 -0.9 578,221 - ABZR87_RS18830 0.62 +0.1 578,275 - ABZR87_RS18830 0.80 +0.5 578,275 - ABZR87_RS18830 0.80 -1.2 578,313 + -1.1 578,313 + +0.8 578,314 - +0.7 578,314 - -0.5 578,369 + -0.3 578,370 - -0.2 578,370 - -0.7 578,421 - +0.8 578,426 + +1.0 578,426 + -0.3 578,427 - -0.6 578,427 - -0.1 578,427 - +0.5 578,427 - -2.5 578,427 - -0.0 578,429 + +0.7 578,465 + +1.0 578,466 - +1.5 578,466 - -0.6 578,471 + -1.7 578,471 + +0.6 578,472 - -2.1 578,472 - -1.3 578,472 - -0.4 578,481 - -1.0 578,600 + ABZR87_RS18835 0.17 +0.4 579,020 + ABZR87_RS18835 0.58 -0.3 579,020 + ABZR87_RS18835 0.58 -2.2 579,020 + ABZR87_RS18835 0.58 -0.2 579,020 + ABZR87_RS18835 0.58 +0.1 579,020 + ABZR87_RS18835 0.58 -0.2 579,020 + ABZR87_RS18835 0.58 -0.4 579,020 + ABZR87_RS18835 0.58 +0.9 579,020 + ABZR87_RS18835 0.58 -3.2 579,020 + ABZR87_RS18835 0.58 +1.1 579,021 - ABZR87_RS18835 0.58 +0.2 579,021 - ABZR87_RS18835 0.58 -1.2 579,021 - ABZR87_RS18835 0.58 -0.3 579,021 - ABZR87_RS18835 0.58 -2.1 579,021 - ABZR87_RS18835 0.58 -0.4 579,021 - ABZR87_RS18835 0.58 +2.1 579,021 - ABZR87_RS18835 0.58 +0.0 579,021 - ABZR87_RS18835 0.58 -0.4 579,021 - ABZR87_RS18835 0.58 -0.4 579,022 + ABZR87_RS18835 0.58 -1.8 579,023 - ABZR87_RS18835 0.58 -0.3 579,023 - ABZR87_RS18835 0.58 -2.4 579,071 + ABZR87_RS18835 0.63 -0.5 579,071 + ABZR87_RS18835 0.63 +0.2 579,071 + ABZR87_RS18835 0.63 -1.0 579,072 - ABZR87_RS18835 0.63 -0.8 579,072 - ABZR87_RS18835 0.63 +0.2 579,072 - ABZR87_RS18835 0.63 -0.3 579,072 - ABZR87_RS18835 0.63 -1.7 579,072 - ABZR87_RS18835 0.63 -1.3 579,179 + ABZR87_RS18835 0.73 -1.0 579,180 - ABZR87_RS18835 0.73 -1.3 579,180 - ABZR87_RS18835 0.73 +1.2 579,180 - ABZR87_RS18835 0.73 -0.6 579,350 + -0.4 579,368 + +0.2 579,368 + +1.2 579,368 + -0.5 579,368 + -1.6 579,368 + -2.1 579,369 - +0.2 579,565 + +0.1 579,565 + -1.8 579,565 + -0.6 579,566 - +0.5 579,566 - +0.4 579,583 + -1.1 579,583 + +0.8 579,604 + ABZR87_RS18840 0.11 -0.9 579,604 + ABZR87_RS18840 0.11 +0.2 579,604 + ABZR87_RS18840 0.11 +1.1 579,612 + ABZR87_RS18840 0.12 -0.2 579,612 + ABZR87_RS18840 0.12 +0.1 579,612 + ABZR87_RS18840 0.12 -0.2 579,612 + ABZR87_RS18840 0.12 -0.4 579,613 - ABZR87_RS18840 0.12 -0.5 579,616 + ABZR87_RS18840 0.12 -1.1 579,617 - ABZR87_RS18840 0.12 -0.0 579,688 + ABZR87_RS18840 0.17 +0.1 579,689 - ABZR87_RS18840 0.17 -3.0 579,689 - ABZR87_RS18840 0.17 -1.7 579,724 + ABZR87_RS18840 0.20 -0.1 579,724 + ABZR87_RS18840 0.20 +0.5 579,724 + ABZR87_RS18840 0.20 -0.0 579,724 + ABZR87_RS18840 0.20 +0.1 579,724 + ABZR87_RS18840 0.20 -1.3 579,725 - ABZR87_RS18840 0.20 -0.7 579,725 - ABZR87_RS18840 0.20 -0.6 579,725 - ABZR87_RS18840 0.20 -2.3 579,725 - ABZR87_RS18840 0.20 +0.1 579,961 + ABZR87_RS18840 0.37 -0.0 579,961 + ABZR87_RS18840 0.37 -0.1 580,229 - ABZR87_RS18840 0.56 -1.4 580,367 - ABZR87_RS18840 0.66 -1.8
Or see this region's nucleotide sequence