Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS18640 and ABZR87_RS18645 are separated by 100 nucleotides ABZR87_RS18645 and ABZR87_RS18650 are separated by 141 nucleotides ABZR87_RS18650 and ABZR87_RS18655 are separated by 81 nucleotides ABZR87_RS18655 and ABZR87_RS18660 overlap by 4 nucleotides
ABZR87_RS18640: ABZR87_RS18640 - ATP-dependent helicase, at 534,872 to 537,022
_RS18640
ABZR87_RS18645: ABZR87_RS18645 - hypothetical protein, at 537,123 to 537,449
_RS18645
ABZR87_RS18650: ABZR87_RS18650 - YnfA family protein, at 537,591 to 537,923
_RS18650
ABZR87_RS18655: ABZR87_RS18655 - carbohydrate kinase, at 538,005 to 538,952
_RS18655
ABZR87_RS18660: ABZR87_RS18660 - xylulokinase, at 538,949 to 540,457
_RS18660
Position (kb)
538
539 Strain fitness (log2 ratio)
-2
-1
0
1 at 537.043 kb on - strand at 537.049 kb on - strand at 537.056 kb on + strand at 537.260 kb on + strand, within ABZR87_RS18645 at 537.543 kb on + strand at 537.543 kb on + strand at 537.543 kb on + strand at 537.544 kb on - strand at 537.570 kb on + strand at 537.570 kb on + strand at 537.575 kb on - strand at 537.716 kb on + strand, within ABZR87_RS18650 at 537.717 kb on - strand, within ABZR87_RS18650 at 537.718 kb on + strand, within ABZR87_RS18650 at 537.718 kb on + strand, within ABZR87_RS18650 at 537.718 kb on + strand, within ABZR87_RS18650 at 537.718 kb on + strand, within ABZR87_RS18650 at 537.718 kb on + strand, within ABZR87_RS18650 at 537.718 kb on + strand, within ABZR87_RS18650 at 537.718 kb on + strand, within ABZR87_RS18650 at 537.718 kb on + strand, within ABZR87_RS18650 at 537.718 kb on + strand, within ABZR87_RS18650 at 537.718 kb on + strand, within ABZR87_RS18650 at 537.719 kb on - strand, within ABZR87_RS18650 at 537.719 kb on - strand, within ABZR87_RS18650 at 537.719 kb on - strand, within ABZR87_RS18650 at 537.719 kb on - strand, within ABZR87_RS18650 at 537.719 kb on - strand, within ABZR87_RS18650 at 537.719 kb on - strand, within ABZR87_RS18650 at 537.719 kb on - strand, within ABZR87_RS18650 at 537.719 kb on - strand, within ABZR87_RS18650 at 537.719 kb on - strand, within ABZR87_RS18650 at 537.719 kb on - strand, within ABZR87_RS18650 at 537.719 kb on - strand, within ABZR87_RS18650 at 537.719 kb on - strand, within ABZR87_RS18650 at 537.739 kb on + strand, within ABZR87_RS18650 at 537.739 kb on + strand, within ABZR87_RS18650 at 537.739 kb on + strand, within ABZR87_RS18650 at 537.740 kb on - strand, within ABZR87_RS18650 at 537.740 kb on - strand, within ABZR87_RS18650 at 537.816 kb on + strand, within ABZR87_RS18650 at 537.817 kb on - strand, within ABZR87_RS18650 at 537.817 kb on - strand, within ABZR87_RS18650 at 537.859 kb on + strand, within ABZR87_RS18650 at 537.859 kb on + strand, within ABZR87_RS18650 at 537.860 kb on - strand, within ABZR87_RS18650 at 537.860 kb on - strand, within ABZR87_RS18650 at 537.860 kb on - strand, within ABZR87_RS18650 at 537.860 kb on - strand, within ABZR87_RS18650 at 537.909 kb on - strand at 537.909 kb on - strand at 537.949 kb on + strand at 537.949 kb on + strand at 538.235 kb on + strand, within ABZR87_RS18655 at 538.236 kb on - strand, within ABZR87_RS18655 at 538.657 kb on + strand, within ABZR87_RS18655 at 538.657 kb on + strand, within ABZR87_RS18655 at 538.658 kb on - strand, within ABZR87_RS18655 at 538.721 kb on - strand, within ABZR87_RS18655 at 538.897 kb on + strand at 539.079 kb on + strand at 539.079 kb on + strand at 539.442 kb on + strand, within ABZR87_RS18660 at 539.442 kb on + strand, within ABZR87_RS18660 at 539.443 kb on - strand, within ABZR87_RS18660 at 539.443 kb on - strand, within ABZR87_RS18660 at 539.768 kb on + strand, within ABZR87_RS18660 at 539.905 kb on + strand, within ABZR87_RS18660 at 539.905 kb on + strand, within ABZR87_RS18660 at 539.905 kb on + strand, within ABZR87_RS18660 at 539.905 kb on + strand, within ABZR87_RS18660 at 539.905 kb on + strand, within ABZR87_RS18660 at 539.906 kb on - strand, within ABZR87_RS18660 at 539.906 kb on - strand, within ABZR87_RS18660 at 539.906 kb on - strand, within ABZR87_RS18660 at 539.906 kb on - strand, within ABZR87_RS18660
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 537,043 - -0.1 537,049 - +0.9 537,056 + -0.5 537,260 + ABZR87_RS18645 0.42 +0.3 537,543 + +0.8 537,543 + -0.5 537,543 + +0.5 537,544 - -0.3 537,570 + -1.8 537,570 + -0.5 537,575 - +0.9 537,716 + ABZR87_RS18650 0.38 +0.4 537,717 - ABZR87_RS18650 0.38 -0.5 537,718 + ABZR87_RS18650 0.38 -0.5 537,718 + ABZR87_RS18650 0.38 +0.7 537,718 + ABZR87_RS18650 0.38 -0.7 537,718 + ABZR87_RS18650 0.38 -0.3 537,718 + ABZR87_RS18650 0.38 -0.2 537,718 + ABZR87_RS18650 0.38 -1.6 537,718 + ABZR87_RS18650 0.38 +0.9 537,718 + ABZR87_RS18650 0.38 -0.3 537,718 + ABZR87_RS18650 0.38 +0.4 537,718 + ABZR87_RS18650 0.38 -1.0 537,719 - ABZR87_RS18650 0.38 +1.1 537,719 - ABZR87_RS18650 0.38 +0.5 537,719 - ABZR87_RS18650 0.38 +0.1 537,719 - ABZR87_RS18650 0.38 -0.6 537,719 - ABZR87_RS18650 0.38 -0.3 537,719 - ABZR87_RS18650 0.38 -0.1 537,719 - ABZR87_RS18650 0.38 +0.2 537,719 - ABZR87_RS18650 0.38 +1.5 537,719 - ABZR87_RS18650 0.38 -1.8 537,719 - ABZR87_RS18650 0.38 -0.3 537,719 - ABZR87_RS18650 0.38 +1.0 537,719 - ABZR87_RS18650 0.38 -0.0 537,739 + ABZR87_RS18650 0.44 +1.5 537,739 + ABZR87_RS18650 0.44 +0.2 537,739 + ABZR87_RS18650 0.44 -0.3 537,740 - ABZR87_RS18650 0.45 +0.6 537,740 - ABZR87_RS18650 0.45 -0.8 537,816 + ABZR87_RS18650 0.68 -0.5 537,817 - ABZR87_RS18650 0.68 +0.3 537,817 - ABZR87_RS18650 0.68 +1.1 537,859 + ABZR87_RS18650 0.80 +0.6 537,859 + ABZR87_RS18650 0.80 +0.6 537,860 - ABZR87_RS18650 0.81 +0.1 537,860 - ABZR87_RS18650 0.81 +0.2 537,860 - ABZR87_RS18650 0.81 -0.3 537,860 - ABZR87_RS18650 0.81 -0.8 537,909 - -2.6 537,909 - -0.1 537,949 + -1.7 537,949 + +0.5 538,235 + ABZR87_RS18655 0.24 +0.1 538,236 - ABZR87_RS18655 0.24 +0.4 538,657 + ABZR87_RS18655 0.69 -0.2 538,657 + ABZR87_RS18655 0.69 -0.4 538,658 - ABZR87_RS18655 0.69 +0.4 538,721 - ABZR87_RS18655 0.76 +0.3 538,897 + -1.0 539,079 + +0.7 539,079 + -0.3 539,442 + ABZR87_RS18660 0.33 -0.8 539,442 + ABZR87_RS18660 0.33 +0.3 539,443 - ABZR87_RS18660 0.33 +0.8 539,443 - ABZR87_RS18660 0.33 +0.6 539,768 + ABZR87_RS18660 0.54 +0.8 539,905 + ABZR87_RS18660 0.63 +1.6 539,905 + ABZR87_RS18660 0.63 +0.1 539,905 + ABZR87_RS18660 0.63 -0.4 539,905 + ABZR87_RS18660 0.63 +0.6 539,905 + ABZR87_RS18660 0.63 -0.4 539,906 - ABZR87_RS18660 0.63 +0.5 539,906 - ABZR87_RS18660 0.63 +0.9 539,906 - ABZR87_RS18660 0.63 -0.5 539,906 - ABZR87_RS18660 0.63 -0.1
Or see this region's nucleotide sequence