Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS18130 and ABZR87_RS18135 are separated by 296 nucleotides ABZR87_RS18135 and ABZR87_RS18140 overlap by 8 nucleotides ABZR87_RS18140 and ABZR87_RS18145 are separated by 24 nucleotides
ABZR87_RS18130: ABZR87_RS18130 - type III secretion system export apparatus subunit SctV, at 430,025 to 432,220
_RS18130
ABZR87_RS18135: ABZR87_RS18135 - FliM/FliN family flagellar motor switch protein, at 432,517 to 433,680
_RS18135
ABZR87_RS18140: ABZR87_RS18140 - type III secretion system export apparatus subunit SctR, at 433,673 to 434,332
_RS18140
ABZR87_RS18145: ABZR87_RS18145 - lytic transglycosylase domain-containing protein, at 434,357 to 435,109
_RS18145
Position (kb) 432
433
434 Strain fitness (log2 ratio) -2
-1
0
1 at 431.869 kb on + strand, within ABZR87_RS18130 at 431.950 kb on + strand, within ABZR87_RS18130 at 432.000 kb on + strand, within ABZR87_RS18130 at 432.001 kb on - strand at 432.229 kb on + strand at 432.230 kb on - strand at 432.239 kb on + strand at 432.239 kb on + strand at 432.240 kb on - strand at 432.240 kb on - strand at 432.240 kb on - strand at 432.338 kb on + strand at 432.338 kb on + strand at 432.339 kb on - strand at 432.339 kb on - strand at 432.443 kb on + strand at 432.444 kb on - strand at 432.595 kb on + strand at 432.595 kb on + strand at 432.596 kb on - strand at 432.596 kb on - strand at 433.679 kb on - strand at 433.760 kb on + strand, within ABZR87_RS18140 at 433.760 kb on + strand, within ABZR87_RS18140 at 434.097 kb on + strand, within ABZR87_RS18140 at 434.097 kb on + strand, within ABZR87_RS18140 at 434.120 kb on - strand, within ABZR87_RS18140 at 434.325 kb on - strand at 434.630 kb on + strand, within ABZR87_RS18145 at 434.631 kb on - strand, within ABZR87_RS18145
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 431,869 + ABZR87_RS18130 0.84 +0.8 431,950 + ABZR87_RS18130 0.88 -0.6 432,000 + ABZR87_RS18130 0.90 +0.4 432,001 - +0.0 432,229 + -0.9 432,230 - -1.1 432,239 + +0.4 432,239 + -0.0 432,240 - -1.3 432,240 - +0.3 432,240 - -0.5 432,338 + -2.5 432,338 + +0.5 432,339 - -0.1 432,339 - +0.4 432,443 + +0.6 432,444 - -0.5 432,595 + +0.9 432,595 + -0.7 432,596 - -0.5 432,596 - +0.0 433,679 - +0.1 433,760 + ABZR87_RS18140 0.13 +0.2 433,760 + ABZR87_RS18140 0.13 -1.2 434,097 + ABZR87_RS18140 0.64 -0.3 434,097 + ABZR87_RS18140 0.64 -0.4 434,120 - ABZR87_RS18140 0.68 -2.2 434,325 - +0.1 434,630 + ABZR87_RS18145 0.36 -0.3 434,631 - ABZR87_RS18145 0.36 +0.8
Or see this region's nucleotide sequence