Strain Fitness in Ralstonia sp. UNC404CL21Col around ABZR87_RS17145

Experiment: Acanthamoeba sp., muxed culture-2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABZR87_RS17140 and ABZR87_RS17145 are separated by 26 nucleotidesABZR87_RS17145 and ABZR87_RS17150 are separated by 47 nucleotidesABZR87_RS17150 and ABZR87_RS17155 overlap by 4 nucleotides ABZR87_RS17140: ABZR87_RS17140 - LysR family transcriptional regulator, at 190,872 to 191,789 _RS17140 ABZR87_RS17145: ABZR87_RS17145 - AMP nucleosidase, at 191,816 to 193,297 _RS17145 ABZR87_RS17150: ABZR87_RS17150 - ABC transporter permease subunit, at 193,345 to 194,118 _RS17150 ABZR87_RS17155: ABZR87_RS17155 - amino acid ABC transporter permease, at 194,115 to 194,825 _RS17155 Position (kb) 191 192 193 194Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 190.862 kb on + strandat 190.871 kb on + strandat 190.871 kb on + strandat 190.871 kb on + strandat 190.871 kb on + strandat 190.872 kb on - strandat 190.872 kb on - strandat 190.908 kb on + strandat 191.048 kb on + strand, within ABZR87_RS17140at 191.325 kb on + strand, within ABZR87_RS17140at 191.326 kb on - strand, within ABZR87_RS17140at 191.326 kb on - strand, within ABZR87_RS17140at 191.361 kb on + strand, within ABZR87_RS17140at 191.941 kb on - strandat 192.015 kb on + strand, within ABZR87_RS17145at 192.015 kb on + strand, within ABZR87_RS17145at 192.102 kb on + strand, within ABZR87_RS17145at 192.103 kb on - strand, within ABZR87_RS17145at 192.115 kb on - strand, within ABZR87_RS17145at 192.115 kb on - strand, within ABZR87_RS17145at 192.115 kb on - strand, within ABZR87_RS17145at 192.115 kb on - strand, within ABZR87_RS17145at 192.160 kb on + strand, within ABZR87_RS17145at 192.303 kb on + strand, within ABZR87_RS17145at 192.303 kb on + strand, within ABZR87_RS17145at 192.303 kb on + strand, within ABZR87_RS17145at 192.303 kb on + strand, within ABZR87_RS17145at 192.303 kb on + strand, within ABZR87_RS17145at 192.303 kb on + strand, within ABZR87_RS17145at 192.304 kb on - strand, within ABZR87_RS17145at 192.304 kb on - strand, within ABZR87_RS17145at 192.304 kb on - strand, within ABZR87_RS17145at 192.321 kb on + strand, within ABZR87_RS17145at 192.322 kb on - strand, within ABZR87_RS17145at 192.322 kb on - strand, within ABZR87_RS17145at 192.322 kb on - strand, within ABZR87_RS17145at 192.585 kb on + strand, within ABZR87_RS17145at 192.585 kb on + strand, within ABZR87_RS17145at 192.585 kb on + strand, within ABZR87_RS17145at 192.585 kb on + strand, within ABZR87_RS17145at 192.585 kb on + strand, within ABZR87_RS17145at 192.586 kb on - strand, within ABZR87_RS17145at 192.586 kb on - strand, within ABZR87_RS17145at 192.586 kb on - strand, within ABZR87_RS17145at 192.586 kb on - strand, within ABZR87_RS17145at 192.586 kb on - strand, within ABZR87_RS17145at 192.586 kb on - strand, within ABZR87_RS17145at 192.645 kb on + strand, within ABZR87_RS17145at 192.646 kb on - strand, within ABZR87_RS17145at 192.655 kb on - strand, within ABZR87_RS17145at 192.705 kb on + strand, within ABZR87_RS17145at 192.706 kb on - strand, within ABZR87_RS17145at 192.706 kb on - strand, within ABZR87_RS17145at 192.706 kb on - strand, within ABZR87_RS17145at 192.711 kb on + strand, within ABZR87_RS17145at 192.711 kb on + strand, within ABZR87_RS17145at 192.712 kb on - strand, within ABZR87_RS17145at 192.712 kb on - strand, within ABZR87_RS17145at 192.712 kb on - strand, within ABZR87_RS17145at 192.789 kb on + strand, within ABZR87_RS17145at 192.789 kb on + strand, within ABZR87_RS17145at 192.790 kb on - strand, within ABZR87_RS17145at 192.790 kb on - strand, within ABZR87_RS17145at 192.790 kb on - strand, within ABZR87_RS17145at 192.790 kb on - strand, within ABZR87_RS17145at 192.790 kb on - strand, within ABZR87_RS17145at 192.839 kb on - strand, within ABZR87_RS17145at 192.839 kb on - strand, within ABZR87_RS17145at 192.957 kb on + strand, within ABZR87_RS17145at 192.957 kb on + strand, within ABZR87_RS17145at 192.958 kb on - strand, within ABZR87_RS17145at 192.958 kb on - strand, within ABZR87_RS17145at 192.980 kb on + strand, within ABZR87_RS17145at 192.981 kb on - strand, within ABZR87_RS17145at 192.987 kb on + strand, within ABZR87_RS17145at 192.990 kb on + strand, within ABZR87_RS17145at 192.990 kb on + strand, within ABZR87_RS17145at 192.990 kb on + strand, within ABZR87_RS17145at 192.991 kb on - strand, within ABZR87_RS17145at 193.029 kb on + strand, within ABZR87_RS17145at 193.041 kb on + strand, within ABZR87_RS17145at 193.110 kb on + strand, within ABZR87_RS17145at 193.116 kb on + strand, within ABZR87_RS17145at 193.116 kb on + strand, within ABZR87_RS17145at 193.117 kb on - strand, within ABZR87_RS17145at 193.117 kb on - strand, within ABZR87_RS17145at 193.200 kb on + strandat 193.200 kb on + strandat 193.201 kb on - strandat 193.201 kb on - strandat 193.276 kb on + strandat 193.276 kb on + strandat 193.309 kb on + strandat 193.310 kb on - strandat 193.346 kb on + strandat 193.346 kb on + strandat 193.347 kb on - strandat 193.347 kb on - strandat 193.347 kb on - strandat 193.490 kb on + strand, within ABZR87_RS17150at 193.490 kb on + strand, within ABZR87_RS17150at 193.490 kb on + strand, within ABZR87_RS17150at 193.491 kb on - strand, within ABZR87_RS17150at 193.491 kb on - strand, within ABZR87_RS17150at 193.513 kb on + strand, within ABZR87_RS17150at 193.513 kb on + strand, within ABZR87_RS17150at 193.514 kb on - strand, within ABZR87_RS17150at 193.589 kb on + strand, within ABZR87_RS17150at 193.589 kb on + strand, within ABZR87_RS17150at 193.589 kb on + strand, within ABZR87_RS17150at 193.589 kb on + strand, within ABZR87_RS17150at 193.650 kb on + strand, within ABZR87_RS17150at 193.650 kb on + strand, within ABZR87_RS17150at 193.650 kb on + strand, within ABZR87_RS17150at 193.651 kb on - strand, within ABZR87_RS17150at 193.651 kb on - strand, within ABZR87_RS17150at 193.651 kb on - strand, within ABZR87_RS17150at 193.775 kb on + strand, within ABZR87_RS17150at 193.775 kb on + strand, within ABZR87_RS17150at 193.775 kb on + strand, within ABZR87_RS17150at 193.775 kb on + strand, within ABZR87_RS17150at 193.776 kb on - strand, within ABZR87_RS17150at 193.776 kb on - strand, within ABZR87_RS17150at 193.776 kb on - strand, within ABZR87_RS17150at 193.860 kb on - strand, within ABZR87_RS17150at 193.860 kb on - strand, within ABZR87_RS17150at 193.860 kb on - strand, within ABZR87_RS17150at 193.882 kb on + strand, within ABZR87_RS17150at 194.084 kb on + strandat 194.085 kb on - strandat 194.085 kb on - strandat 194.215 kb on + strand, within ABZR87_RS17155at 194.215 kb on + strand, within ABZR87_RS17155at 194.215 kb on + strand, within ABZR87_RS17155at 194.215 kb on + strand, within ABZR87_RS17155at 194.215 kb on + strand, within ABZR87_RS17155at 194.215 kb on + strand, within ABZR87_RS17155at 194.215 kb on + strand, within ABZR87_RS17155at 194.215 kb on + strand, within ABZR87_RS17155at 194.216 kb on - strand, within ABZR87_RS17155at 194.216 kb on - strand, within ABZR87_RS17155at 194.216 kb on - strand, within ABZR87_RS17155at 194.216 kb on - strand, within ABZR87_RS17155at 194.282 kb on + strand, within ABZR87_RS17155at 194.283 kb on - strand, within ABZR87_RS17155at 194.284 kb on + strand, within ABZR87_RS17155at 194.285 kb on - strand, within ABZR87_RS17155at 194.285 kb on - strand, within ABZR87_RS17155at 194.285 kb on - strand, within ABZR87_RS17155at 194.285 kb on - strand, within ABZR87_RS17155

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Per-strain Table

Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2
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190,862 + -0.4
190,871 + -0.2
190,871 + -0.2
190,871 + -0.3
190,871 + +1.6
190,872 - +1.3
190,872 - -2.9
190,908 + -0.0
191,048 + ABZR87_RS17140 0.19 -1.1
191,325 + ABZR87_RS17140 0.49 -0.1
191,326 - ABZR87_RS17140 0.49 +0.3
191,326 - ABZR87_RS17140 0.49 +0.6
191,361 + ABZR87_RS17140 0.53 -1.2
191,941 - -0.6
192,015 + ABZR87_RS17145 0.13 -1.6
192,015 + ABZR87_RS17145 0.13 -0.4
192,102 + ABZR87_RS17145 0.19 -0.1
192,103 - ABZR87_RS17145 0.19 +0.0
192,115 - ABZR87_RS17145 0.20 -0.5
192,115 - ABZR87_RS17145 0.20 -0.2
192,115 - ABZR87_RS17145 0.20 -0.5
192,115 - ABZR87_RS17145 0.20 -1.3
192,160 + ABZR87_RS17145 0.23 -0.9
192,303 + ABZR87_RS17145 0.33 -0.9
192,303 + ABZR87_RS17145 0.33 -0.0
192,303 + ABZR87_RS17145 0.33 -0.8
192,303 + ABZR87_RS17145 0.33 -0.6
192,303 + ABZR87_RS17145 0.33 -0.8
192,303 + ABZR87_RS17145 0.33 -1.5
192,304 - ABZR87_RS17145 0.33 -1.3
192,304 - ABZR87_RS17145 0.33 -0.7
192,304 - ABZR87_RS17145 0.33 -2.0
192,321 + ABZR87_RS17145 0.34 -1.1
192,322 - ABZR87_RS17145 0.34 -1.4
192,322 - ABZR87_RS17145 0.34 -0.2
192,322 - ABZR87_RS17145 0.34 -1.9
192,585 + ABZR87_RS17145 0.52 +0.5
192,585 + ABZR87_RS17145 0.52 -1.0
192,585 + ABZR87_RS17145 0.52 -0.0
192,585 + ABZR87_RS17145 0.52 -1.0
192,585 + ABZR87_RS17145 0.52 -1.4
192,586 - ABZR87_RS17145 0.52 +0.5
192,586 - ABZR87_RS17145 0.52 -1.7
192,586 - ABZR87_RS17145 0.52 -0.1
192,586 - ABZR87_RS17145 0.52 -1.1
192,586 - ABZR87_RS17145 0.52 -1.8
192,586 - ABZR87_RS17145 0.52 -0.7
192,645 + ABZR87_RS17145 0.56 +0.5
192,646 - ABZR87_RS17145 0.56 -0.2
192,655 - ABZR87_RS17145 0.57 +1.0
192,705 + ABZR87_RS17145 0.60 -0.2
192,706 - ABZR87_RS17145 0.60 -1.1
192,706 - ABZR87_RS17145 0.60 +1.2
192,706 - ABZR87_RS17145 0.60 -0.4
192,711 + ABZR87_RS17145 0.60 +0.9
192,711 + ABZR87_RS17145 0.60 +0.4
192,712 - ABZR87_RS17145 0.60 +0.2
192,712 - ABZR87_RS17145 0.60 -0.7
192,712 - ABZR87_RS17145 0.60 -0.2
192,789 + ABZR87_RS17145 0.66 -0.4
192,789 + ABZR87_RS17145 0.66 +0.5
192,790 - ABZR87_RS17145 0.66 -1.1
192,790 - ABZR87_RS17145 0.66 +0.5
192,790 - ABZR87_RS17145 0.66 -2.1
192,790 - ABZR87_RS17145 0.66 -1.8
192,790 - ABZR87_RS17145 0.66 -0.4
192,839 - ABZR87_RS17145 0.69 -0.5
192,839 - ABZR87_RS17145 0.69 -0.2
192,957 + ABZR87_RS17145 0.77 -1.4
192,957 + ABZR87_RS17145 0.77 -0.7
192,958 - ABZR87_RS17145 0.77 -0.4
192,958 - ABZR87_RS17145 0.77 +0.1
192,980 + ABZR87_RS17145 0.79 -1.1
192,981 - ABZR87_RS17145 0.79 -0.5
192,987 + ABZR87_RS17145 0.79 +0.5
192,990 + ABZR87_RS17145 0.79 -0.8
192,990 + ABZR87_RS17145 0.79 +0.1
192,990 + ABZR87_RS17145 0.79 -2.2
192,991 - ABZR87_RS17145 0.79 -0.2
193,029 + ABZR87_RS17145 0.82 -1.5
193,041 + ABZR87_RS17145 0.83 -0.0
193,110 + ABZR87_RS17145 0.87 +0.0
193,116 + ABZR87_RS17145 0.88 +1.0
193,116 + ABZR87_RS17145 0.88 +4.4
193,117 - ABZR87_RS17145 0.88 -2.2
193,117 - ABZR87_RS17145 0.88 -1.9
193,200 + -1.4
193,200 + -1.3
193,201 - -1.5
193,201 - -1.0
193,276 + -1.0
193,276 + +0.0
193,309 + -2.8
193,310 - +0.1
193,346 + +0.0
193,346 + -1.0
193,347 - -0.7
193,347 - +0.2
193,347 - -0.0
193,490 + ABZR87_RS17150 0.19 +0.1
193,490 + ABZR87_RS17150 0.19 +0.5
193,490 + ABZR87_RS17150 0.19 -0.3
193,491 - ABZR87_RS17150 0.19 -1.0
193,491 - ABZR87_RS17150 0.19 +0.0
193,513 + ABZR87_RS17150 0.22 +0.4
193,513 + ABZR87_RS17150 0.22 +0.3
193,514 - ABZR87_RS17150 0.22 -2.1
193,589 + ABZR87_RS17150 0.32 -1.1
193,589 + ABZR87_RS17150 0.32 +0.2
193,589 + ABZR87_RS17150 0.32 +0.3
193,589 + ABZR87_RS17150 0.32 +1.1
193,650 + ABZR87_RS17150 0.39 +0.2
193,650 + ABZR87_RS17150 0.39 -0.1
193,650 + ABZR87_RS17150 0.39 -0.8
193,651 - ABZR87_RS17150 0.40 +0.2
193,651 - ABZR87_RS17150 0.40 -2.1
193,651 - ABZR87_RS17150 0.40 -0.5
193,775 + ABZR87_RS17150 0.56 -0.9
193,775 + ABZR87_RS17150 0.56 +1.5
193,775 + ABZR87_RS17150 0.56 -0.6
193,775 + ABZR87_RS17150 0.56 +1.6
193,776 - ABZR87_RS17150 0.56 -1.4
193,776 - ABZR87_RS17150 0.56 +0.4
193,776 - ABZR87_RS17150 0.56 +0.1
193,860 - ABZR87_RS17150 0.67 +0.1
193,860 - ABZR87_RS17150 0.67 -1.4
193,860 - ABZR87_RS17150 0.67 -0.1
193,882 + ABZR87_RS17150 0.69 +0.0
194,084 + +0.2
194,085 - -0.6
194,085 - -1.2
194,215 + ABZR87_RS17155 0.14 +1.1
194,215 + ABZR87_RS17155 0.14 -0.3
194,215 + ABZR87_RS17155 0.14 +1.0
194,215 + ABZR87_RS17155 0.14 +1.1
194,215 + ABZR87_RS17155 0.14 +0.7
194,215 + ABZR87_RS17155 0.14 +0.2
194,215 + ABZR87_RS17155 0.14 -0.2
194,215 + ABZR87_RS17155 0.14 -0.6
194,216 - ABZR87_RS17155 0.14 -0.7
194,216 - ABZR87_RS17155 0.14 -0.6
194,216 - ABZR87_RS17155 0.14 -0.6
194,216 - ABZR87_RS17155 0.14 -0.4
194,282 + ABZR87_RS17155 0.23 +0.2
194,283 - ABZR87_RS17155 0.24 +1.6
194,284 + ABZR87_RS17155 0.24 +2.0
194,285 - ABZR87_RS17155 0.24 +2.5
194,285 - ABZR87_RS17155 0.24 +0.0
194,285 - ABZR87_RS17155 0.24 +0.1
194,285 - ABZR87_RS17155 0.24 -2.2

Or see this region's nucleotide sequence