Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS17055 and ABZR87_RS17060 are separated by 33 nucleotides ABZR87_RS17060 and ABZR87_RS17065 are separated by 128 nucleotides ABZR87_RS17065 and ABZR87_RS17070 are separated by 12 nucleotides ABZR87_RS17070 and ABZR87_RS17075 are separated by 160 nucleotides ABZR87_RS17075 and ABZR87_RS17080 are separated by 172 nucleotides
ABZR87_RS17055: ABZR87_RS17055 - sulfite exporter TauE/SafE family protein, at 176,530 to 177,312
_RS17055
ABZR87_RS17060: ABZR87_RS17060 - carboxymuconolactone decarboxylase family protein, at 177,346 to 177,747
_RS17060
ABZR87_RS17065: ABZR87_RS17065 - hypothetical protein, at 177,876 to 178,208
_RS17065
ABZR87_RS17070: ABZR87_RS17070 - alpha/beta hydrolase, at 178,221 to 179,030
_RS17070
ABZR87_RS17075: ABZR87_RS17075 - acyl-CoA thioesterase, at 179,191 to 179,694
_RS17075
ABZR87_RS17080: ABZR87_RS17080 - DUF2946 family protein, at 179,867 to 180,262
_RS17080
Position (kb) 178
179
180 Strain fitness (log2 ratio) -2
-1
0
1
2 at 177.642 kb on + strand, within ABZR87_RS17060 at 177.643 kb on - strand, within ABZR87_RS17060 at 177.643 kb on - strand, within ABZR87_RS17060 at 177.652 kb on + strand, within ABZR87_RS17060 at 177.652 kb on + strand, within ABZR87_RS17060 at 177.652 kb on + strand, within ABZR87_RS17060 at 177.652 kb on + strand, within ABZR87_RS17060 at 177.653 kb on - strand, within ABZR87_RS17060 at 177.746 kb on - strand at 177.746 kb on - strand at 177.782 kb on + strand at 177.782 kb on + strand at 177.783 kb on - strand at 177.815 kb on - strand at 177.815 kb on - strand at 177.999 kb on + strand, within ABZR87_RS17065 at 178.000 kb on - strand, within ABZR87_RS17065 at 178.000 kb on - strand, within ABZR87_RS17065 at 178.080 kb on + strand, within ABZR87_RS17065 at 178.080 kb on + strand, within ABZR87_RS17065 at 178.081 kb on - strand, within ABZR87_RS17065 at 178.161 kb on + strand, within ABZR87_RS17065 at 178.209 kb on + strand at 178.209 kb on + strand at 178.210 kb on - strand at 178.210 kb on - strand at 178.210 kb on - strand at 178.329 kb on + strand, within ABZR87_RS17070 at 178.330 kb on - strand, within ABZR87_RS17070 at 178.330 kb on - strand, within ABZR87_RS17070 at 178.330 kb on - strand, within ABZR87_RS17070 at 178.330 kb on - strand, within ABZR87_RS17070 at 178.330 kb on - strand, within ABZR87_RS17070 at 178.386 kb on - strand, within ABZR87_RS17070 at 178.491 kb on + strand, within ABZR87_RS17070 at 178.491 kb on + strand, within ABZR87_RS17070 at 178.491 kb on + strand, within ABZR87_RS17070 at 178.491 kb on + strand, within ABZR87_RS17070 at 178.492 kb on - strand, within ABZR87_RS17070 at 178.497 kb on + strand, within ABZR87_RS17070 at 178.498 kb on - strand, within ABZR87_RS17070 at 178.498 kb on - strand, within ABZR87_RS17070 at 178.728 kb on + strand, within ABZR87_RS17070 at 178.729 kb on - strand, within ABZR87_RS17070 at 178.899 kb on + strand, within ABZR87_RS17070 at 178.899 kb on + strand, within ABZR87_RS17070 at 178.899 kb on + strand, within ABZR87_RS17070 at 178.899 kb on + strand, within ABZR87_RS17070 at 178.899 kb on + strand, within ABZR87_RS17070 at 178.900 kb on - strand, within ABZR87_RS17070 at 178.900 kb on - strand, within ABZR87_RS17070 at 178.900 kb on - strand, within ABZR87_RS17070 at 179.028 kb on + strand at 179.073 kb on + strand at 179.074 kb on - strand at 179.085 kb on + strand at 179.085 kb on + strand at 179.137 kb on + strand at 179.137 kb on + strand at 179.138 kb on - strand at 179.192 kb on + strand at 179.193 kb on - strand at 179.193 kb on - strand at 179.193 kb on - strand at 179.216 kb on + strand at 179.217 kb on - strand at 179.217 kb on - strand at 179.375 kb on + strand, within ABZR87_RS17075 at 179.376 kb on - strand, within ABZR87_RS17075 at 179.376 kb on - strand, within ABZR87_RS17075 at 179.376 kb on - strand, within ABZR87_RS17075 at 179.388 kb on + strand, within ABZR87_RS17075 at 179.388 kb on + strand, within ABZR87_RS17075 at 179.388 kb on + strand, within ABZR87_RS17075 at 179.462 kb on + strand, within ABZR87_RS17075 at 179.462 kb on + strand, within ABZR87_RS17075 at 179.462 kb on + strand, within ABZR87_RS17075 at 179.463 kb on - strand, within ABZR87_RS17075 at 179.463 kb on - strand, within ABZR87_RS17075 at 179.463 kb on - strand, within ABZR87_RS17075 at 179.550 kb on - strand, within ABZR87_RS17075 at 179.550 kb on - strand, within ABZR87_RS17075 at 179.579 kb on + strand, within ABZR87_RS17075 at 179.579 kb on + strand, within ABZR87_RS17075 at 179.580 kb on - strand, within ABZR87_RS17075 at 179.580 kb on - strand, within ABZR87_RS17075 at 179.580 kb on - strand, within ABZR87_RS17075 at 179.620 kb on + strand, within ABZR87_RS17075 at 179.620 kb on + strand, within ABZR87_RS17075 at 179.620 kb on + strand, within ABZR87_RS17075 at 179.620 kb on + strand, within ABZR87_RS17075 at 179.688 kb on + strand at 179.689 kb on - strand at 179.689 kb on - strand at 179.689 kb on - strand at 179.819 kb on - strand at 179.823 kb on + strand at 179.823 kb on + strand at 179.823 kb on + strand at 179.824 kb on - strand at 180.008 kb on - strand, within ABZR87_RS17080 at 180.015 kb on + strand, within ABZR87_RS17080
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 177,642 + ABZR87_RS17060 0.74 +0.3 177,643 - ABZR87_RS17060 0.74 -0.2 177,643 - ABZR87_RS17060 0.74 -1.1 177,652 + ABZR87_RS17060 0.76 +0.3 177,652 + ABZR87_RS17060 0.76 +0.1 177,652 + ABZR87_RS17060 0.76 -0.8 177,652 + ABZR87_RS17060 0.76 -0.2 177,653 - ABZR87_RS17060 0.76 -0.3 177,746 - +0.1 177,746 - -0.4 177,782 + -0.6 177,782 + +1.1 177,783 - +1.4 177,815 - +0.3 177,815 - +0.1 177,999 + ABZR87_RS17065 0.37 +0.3 178,000 - ABZR87_RS17065 0.37 -0.0 178,000 - ABZR87_RS17065 0.37 -0.6 178,080 + ABZR87_RS17065 0.61 -0.2 178,080 + ABZR87_RS17065 0.61 +2.0 178,081 - ABZR87_RS17065 0.62 -0.5 178,161 + ABZR87_RS17065 0.86 +0.7 178,209 + -0.2 178,209 + +0.2 178,210 - +1.4 178,210 - +0.4 178,210 - -0.2 178,329 + ABZR87_RS17070 0.13 +0.1 178,330 - ABZR87_RS17070 0.13 -0.2 178,330 - ABZR87_RS17070 0.13 +1.0 178,330 - ABZR87_RS17070 0.13 +1.2 178,330 - ABZR87_RS17070 0.13 -0.2 178,330 - ABZR87_RS17070 0.13 -1.2 178,386 - ABZR87_RS17070 0.20 +0.8 178,491 + ABZR87_RS17070 0.33 -0.3 178,491 + ABZR87_RS17070 0.33 +1.9 178,491 + ABZR87_RS17070 0.33 -0.5 178,491 + ABZR87_RS17070 0.33 +0.3 178,492 - ABZR87_RS17070 0.33 +0.6 178,497 + ABZR87_RS17070 0.34 -0.1 178,498 - ABZR87_RS17070 0.34 +0.0 178,498 - ABZR87_RS17070 0.34 +0.4 178,728 + ABZR87_RS17070 0.63 +0.7 178,729 - ABZR87_RS17070 0.63 +0.4 178,899 + ABZR87_RS17070 0.84 -0.8 178,899 + ABZR87_RS17070 0.84 -0.2 178,899 + ABZR87_RS17070 0.84 +0.5 178,899 + ABZR87_RS17070 0.84 +0.9 178,899 + ABZR87_RS17070 0.84 -0.6 178,900 - ABZR87_RS17070 0.84 -1.0 178,900 - ABZR87_RS17070 0.84 -0.1 178,900 - ABZR87_RS17070 0.84 +0.7 179,028 + +0.4 179,073 + -0.2 179,074 - +0.1 179,085 + -0.5 179,085 + -1.0 179,137 + +0.0 179,137 + +0.2 179,138 - -0.1 179,192 + +2.0 179,193 - -0.7 179,193 - -0.0 179,193 - +0.5 179,216 + +0.4 179,217 - +1.5 179,217 - +0.3 179,375 + ABZR87_RS17075 0.37 -0.5 179,376 - ABZR87_RS17075 0.37 +0.3 179,376 - ABZR87_RS17075 0.37 -0.5 179,376 - ABZR87_RS17075 0.37 +0.3 179,388 + ABZR87_RS17075 0.39 -0.3 179,388 + ABZR87_RS17075 0.39 -1.1 179,388 + ABZR87_RS17075 0.39 -1.2 179,462 + ABZR87_RS17075 0.54 +0.8 179,462 + ABZR87_RS17075 0.54 +0.6 179,462 + ABZR87_RS17075 0.54 -0.7 179,463 - ABZR87_RS17075 0.54 -0.3 179,463 - ABZR87_RS17075 0.54 -1.2 179,463 - ABZR87_RS17075 0.54 -0.8 179,550 - ABZR87_RS17075 0.71 +0.1 179,550 - ABZR87_RS17075 0.71 -0.7 179,579 + ABZR87_RS17075 0.77 +0.6 179,579 + ABZR87_RS17075 0.77 -0.6 179,580 - ABZR87_RS17075 0.77 -0.4 179,580 - ABZR87_RS17075 0.77 -1.0 179,580 - ABZR87_RS17075 0.77 -0.7 179,620 + ABZR87_RS17075 0.85 -0.3 179,620 + ABZR87_RS17075 0.85 -0.5 179,620 + ABZR87_RS17075 0.85 -2.2 179,620 + ABZR87_RS17075 0.85 -0.7 179,688 + +0.2 179,689 - +0.5 179,689 - +0.3 179,689 - +0.4 179,819 - +0.6 179,823 + -2.0 179,823 + -0.3 179,823 + +0.4 179,824 - -0.3 180,008 - ABZR87_RS17080 0.36 +0.5 180,015 + ABZR87_RS17080 0.37 -0.8
Or see this region's nucleotide sequence