Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS16965 and ABZR87_RS16970 overlap by 4 nucleotides ABZR87_RS16970 and ABZR87_RS16975 are separated by 5 nucleotides ABZR87_RS16975 and ABZR87_RS16980 are separated by 141 nucleotides
ABZR87_RS16965: ABZR87_RS16965 - DUF72 domain-containing protein, at 160,668 to 161,543
_RS16965
ABZR87_RS16970: ABZR87_RS16970 - SIR2 family protein, at 161,540 to 162,463
_RS16970
ABZR87_RS16975: ABZR87_RS16975 - hypothetical protein, at 162,469 to 162,627
_RS16975
ABZR87_RS16980: ABZR87_RS16980 - GlxA family transcriptional regulator, at 162,769 to 163,704
_RS16980
Position (kb)
162
163 Strain fitness (log2 ratio)
-1
0
1
2 at 161.485 kb on + strand at 161.486 kb on - strand at 161.486 kb on - strand at 161.510 kb on - strand at 161.658 kb on + strand, within ABZR87_RS16970 at 161.739 kb on + strand, within ABZR87_RS16970 at 161.739 kb on + strand, within ABZR87_RS16970 at 161.739 kb on + strand, within ABZR87_RS16970 at 161.740 kb on - strand, within ABZR87_RS16970 at 161.740 kb on - strand, within ABZR87_RS16970 at 161.772 kb on + strand, within ABZR87_RS16970 at 161.772 kb on + strand, within ABZR87_RS16970 at 161.773 kb on - strand, within ABZR87_RS16970 at 161.865 kb on + strand, within ABZR87_RS16970 at 161.865 kb on + strand, within ABZR87_RS16970 at 161.865 kb on + strand, within ABZR87_RS16970 at 161.865 kb on + strand, within ABZR87_RS16970 at 161.865 kb on + strand, within ABZR87_RS16970 at 161.866 kb on - strand, within ABZR87_RS16970 at 161.866 kb on - strand, within ABZR87_RS16970 at 161.866 kb on - strand, within ABZR87_RS16970 at 161.866 kb on - strand, within ABZR87_RS16970 at 161.878 kb on - strand, within ABZR87_RS16970 at 161.898 kb on + strand, within ABZR87_RS16970 at 161.899 kb on - strand, within ABZR87_RS16970 at 161.899 kb on - strand, within ABZR87_RS16970 at 161.899 kb on - strand, within ABZR87_RS16970 at 162.006 kb on + strand, within ABZR87_RS16970 at 162.007 kb on - strand, within ABZR87_RS16970 at 162.007 kb on - strand, within ABZR87_RS16970 at 162.057 kb on + strand, within ABZR87_RS16970 at 162.410 kb on + strand at 162.411 kb on - strand at 162.569 kb on + strand, within ABZR87_RS16975 at 162.569 kb on + strand, within ABZR87_RS16975 at 162.570 kb on - strand, within ABZR87_RS16975 at 162.570 kb on - strand, within ABZR87_RS16975 at 162.650 kb on + strand at 162.650 kb on + strand at 162.650 kb on + strand at 162.650 kb on + strand at 162.650 kb on + strand at 162.651 kb on - strand at 162.651 kb on - strand at 162.651 kb on - strand at 162.651 kb on - strand at 163.137 kb on - strand, within ABZR87_RS16980 at 163.140 kb on - strand, within ABZR87_RS16980 at 163.140 kb on - strand, within ABZR87_RS16980 at 163.171 kb on + strand, within ABZR87_RS16980 at 163.265 kb on + strand, within ABZR87_RS16980 at 163.265 kb on + strand, within ABZR87_RS16980 at 163.266 kb on - strand, within ABZR87_RS16980 at 163.266 kb on - strand, within ABZR87_RS16980 at 163.266 kb on - strand, within ABZR87_RS16980 at 163.599 kb on - strand, within ABZR87_RS16980 at 163.599 kb on - strand, within ABZR87_RS16980
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 161,485 + -0.3 161,486 - -0.2 161,486 - -1.1 161,510 - +0.2 161,658 + ABZR87_RS16970 0.13 -0.3 161,739 + ABZR87_RS16970 0.22 +0.2 161,739 + ABZR87_RS16970 0.22 +1.1 161,739 + ABZR87_RS16970 0.22 +0.2 161,740 - ABZR87_RS16970 0.22 -0.5 161,740 - ABZR87_RS16970 0.22 +1.2 161,772 + ABZR87_RS16970 0.25 +0.8 161,772 + ABZR87_RS16970 0.25 +0.1 161,773 - ABZR87_RS16970 0.25 +2.1 161,865 + ABZR87_RS16970 0.35 -0.1 161,865 + ABZR87_RS16970 0.35 +0.1 161,865 + ABZR87_RS16970 0.35 +0.8 161,865 + ABZR87_RS16970 0.35 +0.7 161,865 + ABZR87_RS16970 0.35 +1.4 161,866 - ABZR87_RS16970 0.35 -0.2 161,866 - ABZR87_RS16970 0.35 -0.7 161,866 - ABZR87_RS16970 0.35 +0.3 161,866 - ABZR87_RS16970 0.35 -0.1 161,878 - ABZR87_RS16970 0.37 +0.6 161,898 + ABZR87_RS16970 0.39 -0.5 161,899 - ABZR87_RS16970 0.39 -1.1 161,899 - ABZR87_RS16970 0.39 +0.1 161,899 - ABZR87_RS16970 0.39 -1.1 162,006 + ABZR87_RS16970 0.50 +0.4 162,007 - ABZR87_RS16970 0.51 +0.2 162,007 - ABZR87_RS16970 0.51 -0.5 162,057 + ABZR87_RS16970 0.56 -1.2 162,410 + -1.5 162,411 - -0.3 162,569 + ABZR87_RS16975 0.63 -1.6 162,569 + ABZR87_RS16975 0.63 -1.6 162,570 - ABZR87_RS16975 0.64 +0.2 162,570 - ABZR87_RS16975 0.64 +0.0 162,650 + -0.0 162,650 + -0.6 162,650 + +1.1 162,650 + +0.9 162,650 + -0.4 162,651 - +0.5 162,651 - +0.1 162,651 - +0.1 162,651 - -0.4 163,137 - ABZR87_RS16980 0.39 -0.5 163,140 - ABZR87_RS16980 0.40 +0.6 163,140 - ABZR87_RS16980 0.40 +0.3 163,171 + ABZR87_RS16980 0.43 -0.1 163,265 + ABZR87_RS16980 0.53 -0.6 163,265 + ABZR87_RS16980 0.53 +1.2 163,266 - ABZR87_RS16980 0.53 -0.6 163,266 - ABZR87_RS16980 0.53 -0.5 163,266 - ABZR87_RS16980 0.53 +0.1 163,599 - ABZR87_RS16980 0.89 -0.5 163,599 - ABZR87_RS16980 0.89 -0.9
Or see this region's nucleotide sequence