Strain Fitness in Ralstonia sp. UNC404CL21Col around ABZR87_RS16820

Experiment: Acanthamoeba sp., muxed culture-2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABZR87_RS16815 and ABZR87_RS16820 are separated by 146 nucleotidesABZR87_RS16820 and ABZR87_RS16825 are separated by 310 nucleotides ABZR87_RS16815: ABZR87_RS16815 - NAD(P)/FAD-dependent oxidoreductase, at 128,508 to 129,401 _RS16815 ABZR87_RS16820: ABZR87_RS16820 - TetR/AcrR family transcriptional regulator, at 129,548 to 130,186 _RS16820 ABZR87_RS16825: ABZR87_RS16825 - restriction endonuclease, at 130,497 to 131,474 _RS16825 Position (kb) 129 130 131Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 128.611 kb on + strand, within ABZR87_RS16815at 128.611 kb on + strand, within ABZR87_RS16815at 128.611 kb on + strand, within ABZR87_RS16815at 128.611 kb on + strand, within ABZR87_RS16815at 128.611 kb on + strand, within ABZR87_RS16815at 128.611 kb on + strand, within ABZR87_RS16815at 128.611 kb on + strand, within ABZR87_RS16815at 128.612 kb on - strand, within ABZR87_RS16815at 128.612 kb on - strand, within ABZR87_RS16815at 128.868 kb on - strand, within ABZR87_RS16815at 128.944 kb on + strand, within ABZR87_RS16815at 128.945 kb on - strand, within ABZR87_RS16815at 128.945 kb on - strand, within ABZR87_RS16815at 128.945 kb on - strand, within ABZR87_RS16815at 128.945 kb on - strand, within ABZR87_RS16815at 129.008 kb on - strand, within ABZR87_RS16815at 129.008 kb on - strand, within ABZR87_RS16815at 129.082 kb on + strand, within ABZR87_RS16815at 129.082 kb on + strand, within ABZR87_RS16815at 129.083 kb on - strand, within ABZR87_RS16815at 129.083 kb on - strand, within ABZR87_RS16815at 129.083 kb on - strand, within ABZR87_RS16815at 129.234 kb on - strand, within ABZR87_RS16815at 129.309 kb on - strand, within ABZR87_RS16815at 129.509 kb on + strandat 129.541 kb on - strandat 129.690 kb on + strand, within ABZR87_RS16820at 129.690 kb on + strand, within ABZR87_RS16820at 129.690 kb on + strand, within ABZR87_RS16820at 129.690 kb on + strand, within ABZR87_RS16820at 129.707 kb on + strand, within ABZR87_RS16820at 129.707 kb on + strand, within ABZR87_RS16820at 129.708 kb on - strand, within ABZR87_RS16820at 129.708 kb on - strand, within ABZR87_RS16820at 129.708 kb on - strand, within ABZR87_RS16820at 129.708 kb on - strand, within ABZR87_RS16820at 129.935 kb on + strand, within ABZR87_RS16820at 129.936 kb on - strand, within ABZR87_RS16820at 129.936 kb on - strand, within ABZR87_RS16820at 129.936 kb on - strand, within ABZR87_RS16820at 129.936 kb on - strand, within ABZR87_RS16820at 129.978 kb on + strand, within ABZR87_RS16820at 129.979 kb on - strand, within ABZR87_RS16820at 129.979 kb on - strand, within ABZR87_RS16820at 129.979 kb on - strand, within ABZR87_RS16820at 130.078 kb on + strand, within ABZR87_RS16820at 130.078 kb on + strand, within ABZR87_RS16820at 130.079 kb on - strand, within ABZR87_RS16820at 130.079 kb on - strand, within ABZR87_RS16820at 130.079 kb on - strand, within ABZR87_RS16820at 130.123 kb on - strandat 130.130 kb on + strandat 130.130 kb on + strandat 130.131 kb on - strandat 130.131 kb on - strandat 130.196 kb on + strandat 130.196 kb on + strandat 130.196 kb on + strandat 130.196 kb on + strandat 130.196 kb on + strandat 130.197 kb on - strandat 130.197 kb on - strandat 130.197 kb on - strandat 130.197 kb on - strandat 130.197 kb on - strandat 130.236 kb on + strandat 130.327 kb on - strandat 130.327 kb on - strandat 130.327 kb on - strandat 130.395 kb on + strandat 130.395 kb on + strandat 130.395 kb on + strandat 130.395 kb on + strandat 130.395 kb on + strandat 130.396 kb on - strandat 130.396 kb on - strandat 130.396 kb on - strandat 130.396 kb on - strandat 130.396 kb on - strandat 130.396 kb on - strandat 130.396 kb on - strandat 130.463 kb on + strandat 130.463 kb on + strandat 130.464 kb on - strandat 130.471 kb on + strandat 130.481 kb on - strandat 130.495 kb on + strandat 130.534 kb on + strandat 130.534 kb on + strandat 130.535 kb on - strandat 130.584 kb on + strandat 130.585 kb on - strandat 130.585 kb on - strandat 130.713 kb on + strand, within ABZR87_RS16825at 130.713 kb on + strand, within ABZR87_RS16825at 130.713 kb on + strand, within ABZR87_RS16825at 130.713 kb on + strand, within ABZR87_RS16825at 130.714 kb on - strand, within ABZR87_RS16825at 130.714 kb on - strand, within ABZR87_RS16825at 130.714 kb on - strand, within ABZR87_RS16825at 130.989 kb on + strand, within ABZR87_RS16825at 130.989 kb on + strand, within ABZR87_RS16825at 130.989 kb on + strand, within ABZR87_RS16825at 130.990 kb on - strand, within ABZR87_RS16825at 130.990 kb on - strand, within ABZR87_RS16825at 130.990 kb on - strand, within ABZR87_RS16825at 130.992 kb on + strand, within ABZR87_RS16825at 130.992 kb on + strand, within ABZR87_RS16825at 130.993 kb on - strand, within ABZR87_RS16825at 130.993 kb on - strand, within ABZR87_RS16825at 130.993 kb on - strand, within ABZR87_RS16825at 131.009 kb on + strand, within ABZR87_RS16825at 131.009 kb on + strand, within ABZR87_RS16825at 131.010 kb on - strand, within ABZR87_RS16825at 131.061 kb on + strand, within ABZR87_RS16825at 131.061 kb on + strand, within ABZR87_RS16825at 131.061 kb on + strand, within ABZR87_RS16825at 131.061 kb on + strand, within ABZR87_RS16825at 131.061 kb on + strand, within ABZR87_RS16825at 131.061 kb on + strand, within ABZR87_RS16825at 131.061 kb on + strand, within ABZR87_RS16825at 131.062 kb on - strand, within ABZR87_RS16825at 131.062 kb on - strand, within ABZR87_RS16825at 131.062 kb on - strand, within ABZR87_RS16825at 131.062 kb on - strand, within ABZR87_RS16825at 131.062 kb on - strand, within ABZR87_RS16825at 131.062 kb on - strand, within ABZR87_RS16825at 131.062 kb on - strand, within ABZR87_RS16825at 131.062 kb on - strand, within ABZR87_RS16825at 131.062 kb on - strand, within ABZR87_RS16825at 131.062 kb on - strand, within ABZR87_RS16825at 131.062 kb on - strand, within ABZR87_RS16825at 131.062 kb on - strand, within ABZR87_RS16825

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Per-strain Table

Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2
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128,611 + ABZR87_RS16815 0.12 +0.1
128,611 + ABZR87_RS16815 0.12 +0.5
128,611 + ABZR87_RS16815 0.12 +1.1
128,611 + ABZR87_RS16815 0.12 -0.8
128,611 + ABZR87_RS16815 0.12 -1.1
128,611 + ABZR87_RS16815 0.12 -1.0
128,611 + ABZR87_RS16815 0.12 +0.6
128,612 - ABZR87_RS16815 0.12 -0.0
128,612 - ABZR87_RS16815 0.12 -1.4
128,868 - ABZR87_RS16815 0.40 -0.2
128,944 + ABZR87_RS16815 0.49 -0.2
128,945 - ABZR87_RS16815 0.49 +0.9
128,945 - ABZR87_RS16815 0.49 +0.1
128,945 - ABZR87_RS16815 0.49 +0.3
128,945 - ABZR87_RS16815 0.49 -0.2
129,008 - ABZR87_RS16815 0.56 -0.3
129,008 - ABZR87_RS16815 0.56 +1.1
129,082 + ABZR87_RS16815 0.64 -0.6
129,082 + ABZR87_RS16815 0.64 +0.5
129,083 - ABZR87_RS16815 0.64 -0.7
129,083 - ABZR87_RS16815 0.64 +0.3
129,083 - ABZR87_RS16815 0.64 -0.0
129,234 - ABZR87_RS16815 0.81 -0.5
129,309 - ABZR87_RS16815 0.90 -0.1
129,509 + +0.6
129,541 - -0.9
129,690 + ABZR87_RS16820 0.22 +0.4
129,690 + ABZR87_RS16820 0.22 -0.8
129,690 + ABZR87_RS16820 0.22 -1.2
129,690 + ABZR87_RS16820 0.22 +1.1
129,707 + ABZR87_RS16820 0.25 -1.4
129,707 + ABZR87_RS16820 0.25 +0.6
129,708 - ABZR87_RS16820 0.25 +0.3
129,708 - ABZR87_RS16820 0.25 -1.3
129,708 - ABZR87_RS16820 0.25 -1.0
129,708 - ABZR87_RS16820 0.25 -0.0
129,935 + ABZR87_RS16820 0.61 -2.9
129,936 - ABZR87_RS16820 0.61 -0.5
129,936 - ABZR87_RS16820 0.61 -0.9
129,936 - ABZR87_RS16820 0.61 -0.7
129,936 - ABZR87_RS16820 0.61 -0.9
129,978 + ABZR87_RS16820 0.67 +1.6
129,979 - ABZR87_RS16820 0.67 -0.5
129,979 - ABZR87_RS16820 0.67 -0.4
129,979 - ABZR87_RS16820 0.67 +0.6
130,078 + ABZR87_RS16820 0.83 -2.7
130,078 + ABZR87_RS16820 0.83 -0.9
130,079 - ABZR87_RS16820 0.83 -1.9
130,079 - ABZR87_RS16820 0.83 -0.6
130,079 - ABZR87_RS16820 0.83 -0.4
130,123 - -1.9
130,130 + -0.3
130,130 + -1.3
130,131 - -0.1
130,131 - -0.6
130,196 + -0.0
130,196 + -0.8
130,196 + -0.6
130,196 + -0.1
130,196 + +0.3
130,197 - +0.4
130,197 - +1.4
130,197 - -0.2
130,197 - -0.1
130,197 - -1.6
130,236 + +0.2
130,327 - -0.3
130,327 - +1.3
130,327 - +0.7
130,395 + +0.3
130,395 + -2.1
130,395 + -0.8
130,395 + -1.6
130,395 + -1.9
130,396 - +0.1
130,396 - -0.1
130,396 - +0.1
130,396 - -0.4
130,396 - +0.3
130,396 - +0.4
130,396 - +1.3
130,463 + +0.2
130,463 + -0.2
130,464 - +0.1
130,471 + +0.8
130,481 - +0.3
130,495 + -0.0
130,534 + -0.6
130,534 + +0.4
130,535 - -2.2
130,584 + +0.5
130,585 - +0.4
130,585 - +1.0
130,713 + ABZR87_RS16825 0.22 -0.4
130,713 + ABZR87_RS16825 0.22 +0.0
130,713 + ABZR87_RS16825 0.22 +1.2
130,713 + ABZR87_RS16825 0.22 -0.3
130,714 - ABZR87_RS16825 0.22 -0.1
130,714 - ABZR87_RS16825 0.22 -2.3
130,714 - ABZR87_RS16825 0.22 +0.3
130,989 + ABZR87_RS16825 0.50 -0.3
130,989 + ABZR87_RS16825 0.50 -0.5
130,989 + ABZR87_RS16825 0.50 +0.4
130,990 - ABZR87_RS16825 0.50 +0.2
130,990 - ABZR87_RS16825 0.50 -0.8
130,990 - ABZR87_RS16825 0.50 +0.4
130,992 + ABZR87_RS16825 0.51 +0.5
130,992 + ABZR87_RS16825 0.51 -0.7
130,993 - ABZR87_RS16825 0.51 +0.6
130,993 - ABZR87_RS16825 0.51 +2.1
130,993 - ABZR87_RS16825 0.51 -1.1
131,009 + ABZR87_RS16825 0.52 +0.3
131,009 + ABZR87_RS16825 0.52 -0.5
131,010 - ABZR87_RS16825 0.52 +0.7
131,061 + ABZR87_RS16825 0.58 -0.8
131,061 + ABZR87_RS16825 0.58 -0.0
131,061 + ABZR87_RS16825 0.58 +0.7
131,061 + ABZR87_RS16825 0.58 -1.7
131,061 + ABZR87_RS16825 0.58 +0.7
131,061 + ABZR87_RS16825 0.58 -0.9
131,061 + ABZR87_RS16825 0.58 -1.5
131,062 - ABZR87_RS16825 0.58 +1.0
131,062 - ABZR87_RS16825 0.58 +0.4
131,062 - ABZR87_RS16825 0.58 -0.3
131,062 - ABZR87_RS16825 0.58 -0.0
131,062 - ABZR87_RS16825 0.58 -0.6
131,062 - ABZR87_RS16825 0.58 +0.4
131,062 - ABZR87_RS16825 0.58 -0.1
131,062 - ABZR87_RS16825 0.58 -0.5
131,062 - ABZR87_RS16825 0.58 +1.4
131,062 - ABZR87_RS16825 0.58 +1.1
131,062 - ABZR87_RS16825 0.58 -0.7
131,062 - ABZR87_RS16825 0.58 -0.3

Or see this region's nucleotide sequence