Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS16720 and ABZR87_RS16725 are separated by 162 nucleotides ABZR87_RS16725 and ABZR87_RS16730 are separated by 82 nucleotides ABZR87_RS16730 and ABZR87_RS16735 overlap by 4 nucleotides
ABZR87_RS16720: ABZR87_RS16720 - collagen-like triple helix repeat-containing protein, at 100,155 to 101,300
_RS16720
ABZR87_RS16725: ABZR87_RS16725 - antibiotic biosynthesis monooxygenase, at 101,463 to 101,771
_RS16725
ABZR87_RS16730: ABZR87_RS16730 - 2-methylaconitate cis-trans isomerase PrpF, at 101,854 to 103,038
_RS16730
ABZR87_RS16735: ABZR87_RS16735 - Fe/S-dependent 2-methylisocitrate dehydratase AcnD, at 103,035 to 105,632
_RS16735
Position (kb) 101
102
103
104 Strain fitness (log2 ratio) -2
-1
0
1
2 at 100.913 kb on + strand, within ABZR87_RS16720 at 100.914 kb on - strand, within ABZR87_RS16720 at 101.377 kb on + strand at 101.377 kb on + strand at 101.377 kb on + strand at 101.389 kb on + strand at 101.389 kb on + strand at 101.389 kb on + strand at 101.390 kb on - strand at 101.415 kb on + strand at 101.415 kb on + strand at 101.415 kb on + strand at 101.415 kb on + strand at 101.416 kb on - strand at 101.416 kb on - strand at 101.416 kb on - strand at 101.416 kb on - strand at 101.416 kb on - strand at 101.416 kb on - strand at 101.420 kb on + strand at 101.420 kb on + strand at 101.421 kb on - strand at 101.421 kb on - strand at 102.173 kb on + strand, within ABZR87_RS16730 at 102.173 kb on + strand, within ABZR87_RS16730 at 102.173 kb on + strand, within ABZR87_RS16730 at 102.174 kb on - strand, within ABZR87_RS16730 at 102.174 kb on - strand, within ABZR87_RS16730 at 102.174 kb on - strand, within ABZR87_RS16730 at 102.345 kb on - strand, within ABZR87_RS16730 at 102.782 kb on + strand, within ABZR87_RS16730 at 102.782 kb on + strand, within ABZR87_RS16730 at 102.783 kb on - strand, within ABZR87_RS16730 at 102.783 kb on - strand, within ABZR87_RS16730 at 102.783 kb on - strand, within ABZR87_RS16730 at 102.783 kb on - strand, within ABZR87_RS16730 at 103.001 kb on + strand at 103.001 kb on + strand at 103.001 kb on + strand at 103.001 kb on + strand at 103.001 kb on + strand at 103.001 kb on + strand at 103.001 kb on + strand at 103.002 kb on - strand at 103.002 kb on - strand at 103.002 kb on - strand at 103.002 kb on - strand at 103.002 kb on - strand at 103.103 kb on - strand at 103.103 kb on - strand at 103.408 kb on + strand, within ABZR87_RS16735 at 103.453 kb on + strand, within ABZR87_RS16735 at 103.453 kb on + strand, within ABZR87_RS16735 at 103.453 kb on + strand, within ABZR87_RS16735 at 103.453 kb on + strand, within ABZR87_RS16735 at 103.454 kb on - strand, within ABZR87_RS16735 at 103.454 kb on - strand, within ABZR87_RS16735 at 103.454 kb on - strand, within ABZR87_RS16735 at 103.618 kb on + strand, within ABZR87_RS16735 at 103.618 kb on + strand, within ABZR87_RS16735 at 103.619 kb on - strand, within ABZR87_RS16735 at 103.619 kb on - strand, within ABZR87_RS16735 at 103.660 kb on + strand, within ABZR87_RS16735 at 103.660 kb on + strand, within ABZR87_RS16735 at 103.660 kb on + strand, within ABZR87_RS16735 at 103.660 kb on + strand, within ABZR87_RS16735 at 103.792 kb on + strand, within ABZR87_RS16735 at 103.810 kb on + strand, within ABZR87_RS16735 at 103.810 kb on + strand, within ABZR87_RS16735 at 103.810 kb on + strand, within ABZR87_RS16735 at 103.810 kb on + strand, within ABZR87_RS16735 at 103.810 kb on + strand, within ABZR87_RS16735 at 103.810 kb on + strand, within ABZR87_RS16735 at 103.810 kb on + strand, within ABZR87_RS16735 at 103.811 kb on - strand, within ABZR87_RS16735 at 103.811 kb on - strand, within ABZR87_RS16735 at 103.811 kb on - strand, within ABZR87_RS16735 at 103.811 kb on - strand, within ABZR87_RS16735 at 103.811 kb on - strand, within ABZR87_RS16735 at 103.811 kb on - strand, within ABZR87_RS16735 at 103.835 kb on - strand, within ABZR87_RS16735 at 103.888 kb on + strand, within ABZR87_RS16735 at 103.888 kb on + strand, within ABZR87_RS16735 at 103.888 kb on + strand, within ABZR87_RS16735 at 103.888 kb on + strand, within ABZR87_RS16735 at 103.888 kb on + strand, within ABZR87_RS16735 at 103.888 kb on + strand, within ABZR87_RS16735 at 103.888 kb on + strand, within ABZR87_RS16735 at 103.888 kb on + strand, within ABZR87_RS16735 at 103.889 kb on - strand, within ABZR87_RS16735 at 103.889 kb on - strand, within ABZR87_RS16735 at 103.889 kb on - strand, within ABZR87_RS16735 at 103.889 kb on - strand, within ABZR87_RS16735 at 103.889 kb on - strand, within ABZR87_RS16735 at 103.889 kb on - strand, within ABZR87_RS16735 at 103.889 kb on - strand, within ABZR87_RS16735 at 103.890 kb on + strand, within ABZR87_RS16735 at 103.891 kb on - strand, within ABZR87_RS16735 at 103.891 kb on - strand, within ABZR87_RS16735
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 100,913 + ABZR87_RS16720 0.66 -0.5 100,914 - ABZR87_RS16720 0.66 +1.1 101,377 + -0.1 101,377 + +0.5 101,377 + +1.5 101,389 + -0.3 101,389 + +0.1 101,389 + -0.2 101,390 - +1.9 101,415 + -0.9 101,415 + +0.2 101,415 + +0.3 101,415 + -0.2 101,416 - -0.2 101,416 - +0.0 101,416 - +0.2 101,416 - -0.8 101,416 - -0.1 101,416 - -1.3 101,420 + -0.3 101,420 + -1.1 101,421 - +0.2 101,421 - -0.0 102,173 + ABZR87_RS16730 0.27 +0.5 102,173 + ABZR87_RS16730 0.27 -1.7 102,173 + ABZR87_RS16730 0.27 -0.4 102,174 - ABZR87_RS16730 0.27 +0.2 102,174 - ABZR87_RS16730 0.27 -0.9 102,174 - ABZR87_RS16730 0.27 -1.3 102,345 - ABZR87_RS16730 0.41 -1.9 102,782 + ABZR87_RS16730 0.78 -0.7 102,782 + ABZR87_RS16730 0.78 -1.1 102,783 - ABZR87_RS16730 0.78 -0.2 102,783 - ABZR87_RS16730 0.78 +0.2 102,783 - ABZR87_RS16730 0.78 +1.3 102,783 - ABZR87_RS16730 0.78 -2.1 103,001 + -0.6 103,001 + -0.4 103,001 + -0.9 103,001 + -0.6 103,001 + +0.7 103,001 + +0.7 103,001 + -0.2 103,002 - +0.5 103,002 - +0.9 103,002 - -1.0 103,002 - -1.5 103,002 - -1.4 103,103 - -2.0 103,103 - -0.1 103,408 + ABZR87_RS16735 0.14 -0.6 103,453 + ABZR87_RS16735 0.16 -1.3 103,453 + ABZR87_RS16735 0.16 -1.5 103,453 + ABZR87_RS16735 0.16 -1.5 103,453 + ABZR87_RS16735 0.16 -0.9 103,454 - ABZR87_RS16735 0.16 -0.2 103,454 - ABZR87_RS16735 0.16 +0.1 103,454 - ABZR87_RS16735 0.16 -0.4 103,618 + ABZR87_RS16735 0.22 -0.5 103,618 + ABZR87_RS16735 0.22 -0.3 103,619 - ABZR87_RS16735 0.22 -1.8 103,619 - ABZR87_RS16735 0.22 -0.2 103,660 + ABZR87_RS16735 0.24 -0.3 103,660 + ABZR87_RS16735 0.24 +0.1 103,660 + ABZR87_RS16735 0.24 -0.3 103,660 + ABZR87_RS16735 0.24 -1.1 103,792 + ABZR87_RS16735 0.29 -0.5 103,810 + ABZR87_RS16735 0.30 -0.8 103,810 + ABZR87_RS16735 0.30 -0.1 103,810 + ABZR87_RS16735 0.30 -0.5 103,810 + ABZR87_RS16735 0.30 -0.5 103,810 + ABZR87_RS16735 0.30 -0.7 103,810 + ABZR87_RS16735 0.30 -0.1 103,810 + ABZR87_RS16735 0.30 -0.2 103,811 - ABZR87_RS16735 0.30 -0.4 103,811 - ABZR87_RS16735 0.30 -0.5 103,811 - ABZR87_RS16735 0.30 +0.7 103,811 - ABZR87_RS16735 0.30 +0.2 103,811 - ABZR87_RS16735 0.30 -2.0 103,811 - ABZR87_RS16735 0.30 -0.4 103,835 - ABZR87_RS16735 0.31 -0.3 103,888 + ABZR87_RS16735 0.33 -0.1 103,888 + ABZR87_RS16735 0.33 -0.1 103,888 + ABZR87_RS16735 0.33 -0.4 103,888 + ABZR87_RS16735 0.33 -0.4 103,888 + ABZR87_RS16735 0.33 -1.4 103,888 + ABZR87_RS16735 0.33 +0.4 103,888 + ABZR87_RS16735 0.33 -0.2 103,888 + ABZR87_RS16735 0.33 -0.7 103,889 - ABZR87_RS16735 0.33 +0.2 103,889 - ABZR87_RS16735 0.33 +0.2 103,889 - ABZR87_RS16735 0.33 -0.5 103,889 - ABZR87_RS16735 0.33 +0.2 103,889 - ABZR87_RS16735 0.33 -0.4 103,889 - ABZR87_RS16735 0.33 +0.1 103,889 - ABZR87_RS16735 0.33 +0.7 103,890 + ABZR87_RS16735 0.33 -0.5 103,891 - ABZR87_RS16735 0.33 -0.4 103,891 - ABZR87_RS16735 0.33 +0.2
Or see this region's nucleotide sequence