Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS16100 and ABZR87_RS16105 are separated by 300 nucleotides ABZR87_RS16105 and ABZR87_RS16110 are separated by 169 nucleotides ABZR87_RS16110 and ABZR87_RS16115 are separated by 41 nucleotides ABZR87_RS16115 and ABZR87_RS16120 are separated by 122 nucleotides
ABZR87_RS16100: ABZR87_RS16100 - hypothetical protein, at 3,403,681 to 3,403,872
_RS16100
ABZR87_RS16105: ABZR87_RS16105 - inorganic diphosphatase, at 3,404,173 to 3,404,700
_RS16105
ABZR87_RS16110: ABZR87_RS16110 - aldehyde dehydrogenase family protein, at 3,404,870 to 3,406,306
_RS16110
ABZR87_RS16115: ABZR87_RS16115 - SDR family oxidoreductase, at 3,406,348 to 3,407,106
_RS16115
ABZR87_RS16120: ABZR87_RS16120 - MFS transporter, at 3,407,229 to 3,408,563
_RS16120
Position (kb)
3404
3405
3406
3407 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3403.878 kb on + strand at 3403.879 kb on - strand at 3403.912 kb on + strand at 3403.912 kb on + strand at 3403.913 kb on - strand at 3403.913 kb on - strand at 3403.950 kb on - strand at 3403.950 kb on - strand at 3403.981 kb on - strand at 3403.981 kb on - strand at 3403.984 kb on + strand at 3404.018 kb on + strand at 3404.018 kb on + strand at 3404.018 kb on + strand at 3404.018 kb on + strand at 3404.019 kb on - strand at 3404.019 kb on - strand at 3404.019 kb on - strand at 3404.019 kb on - strand at 3404.019 kb on - strand at 3404.019 kb on - strand at 3404.019 kb on - strand at 3404.019 kb on - strand at 3404.019 kb on - strand at 3404.019 kb on - strand at 3404.019 kb on - strand at 3404.019 kb on - strand at 3404.164 kb on + strand at 3404.165 kb on - strand at 3404.174 kb on + strand at 3404.174 kb on + strand at 3404.174 kb on + strand at 3404.175 kb on - strand at 3404.175 kb on - strand at 3404.181 kb on + strand at 3404.181 kb on + strand at 3404.182 kb on - strand at 3404.182 kb on - strand at 3404.182 kb on - strand at 3404.763 kb on - strand at 3404.784 kb on - strand at 3404.785 kb on + strand at 3404.785 kb on + strand at 3404.785 kb on + strand at 3404.785 kb on + strand at 3404.785 kb on + strand at 3404.785 kb on + strand at 3404.785 kb on + strand at 3404.786 kb on - strand at 3404.786 kb on - strand at 3404.786 kb on - strand at 3404.786 kb on - strand at 3404.786 kb on - strand at 3404.993 kb on - strand at 3405.085 kb on - strand, within ABZR87_RS16110 at 3405.085 kb on - strand, within ABZR87_RS16110 at 3405.191 kb on + strand, within ABZR87_RS16110 at 3405.203 kb on + strand, within ABZR87_RS16110 at 3405.203 kb on + strand, within ABZR87_RS16110 at 3405.203 kb on + strand, within ABZR87_RS16110 at 3405.256 kb on + strand, within ABZR87_RS16110 at 3405.256 kb on + strand, within ABZR87_RS16110 at 3405.257 kb on - strand, within ABZR87_RS16110 at 3405.317 kb on + strand, within ABZR87_RS16110 at 3405.479 kb on + strand, within ABZR87_RS16110 at 3405.749 kb on + strand, within ABZR87_RS16110 at 3405.749 kb on + strand, within ABZR87_RS16110 at 3405.749 kb on + strand, within ABZR87_RS16110 at 3405.749 kb on + strand, within ABZR87_RS16110 at 3405.750 kb on - strand, within ABZR87_RS16110 at 3405.750 kb on - strand, within ABZR87_RS16110 at 3405.950 kb on + strand, within ABZR87_RS16110 at 3405.950 kb on + strand, within ABZR87_RS16110 at 3405.950 kb on + strand, within ABZR87_RS16110 at 3405.950 kb on + strand, within ABZR87_RS16110 at 3405.951 kb on - strand, within ABZR87_RS16110 at 3405.951 kb on - strand, within ABZR87_RS16110 at 3405.951 kb on - strand, within ABZR87_RS16110 at 3405.951 kb on - strand, within ABZR87_RS16110 at 3406.085 kb on + strand, within ABZR87_RS16110 at 3406.086 kb on - strand, within ABZR87_RS16110 at 3406.262 kb on + strand at 3406.263 kb on - strand at 3406.263 kb on - strand at 3406.304 kb on + strand at 3406.586 kb on - strand, within ABZR87_RS16115 at 3406.595 kb on + strand, within ABZR87_RS16115 at 3406.599 kb on + strand, within ABZR87_RS16115 at 3406.599 kb on + strand, within ABZR87_RS16115 at 3406.599 kb on + strand, within ABZR87_RS16115 at 3406.599 kb on + strand, within ABZR87_RS16115 at 3406.600 kb on - strand, within ABZR87_RS16115 at 3406.600 kb on - strand, within ABZR87_RS16115 at 3406.702 kb on + strand, within ABZR87_RS16115 at 3406.702 kb on + strand, within ABZR87_RS16115 at 3406.702 kb on + strand, within ABZR87_RS16115 at 3406.703 kb on - strand, within ABZR87_RS16115 at 3406.810 kb on + strand, within ABZR87_RS16115 at 3406.811 kb on - strand, within ABZR87_RS16115 at 3406.943 kb on + strand, within ABZR87_RS16115 at 3407.035 kb on + strand at 3407.036 kb on - strand at 3407.036 kb on - strand at 3407.036 kb on - strand at 3407.036 kb on - strand at 3407.102 kb on + strand at 3407.102 kb on + strand at 3407.102 kb on + strand at 3407.103 kb on - strand at 3407.103 kb on - strand at 3407.103 kb on - strand at 3407.105 kb on + strand at 3407.105 kb on - strand at 3407.105 kb on - strand at 3407.179 kb on + strand at 3407.179 kb on + strand at 3407.180 kb on - strand at 3407.183 kb on + strand at 3407.183 kb on + strand at 3407.183 kb on + strand at 3407.213 kb on + strand at 3407.214 kb on - strand at 3407.214 kb on - strand at 3407.214 kb on - strand at 3407.214 kb on - strand at 3407.273 kb on + strand at 3407.273 kb on + strand at 3407.273 kb on + strand at 3407.273 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 3,403,878 + +0.7 3,403,879 - -1.9 3,403,912 + -0.1 3,403,912 + -0.7 3,403,913 - +0.4 3,403,913 - -0.1 3,403,950 - +0.2 3,403,950 - -1.2 3,403,981 - +2.0 3,403,981 - +0.1 3,403,984 + -0.5 3,404,018 + -1.5 3,404,018 + +1.0 3,404,018 + -0.7 3,404,018 + +0.2 3,404,019 - +1.2 3,404,019 - +0.4 3,404,019 - -0.5 3,404,019 - +0.2 3,404,019 - -0.4 3,404,019 - -0.1 3,404,019 - +1.3 3,404,019 - +0.3 3,404,019 - -0.2 3,404,019 - +0.8 3,404,019 - +1.1 3,404,019 - +0.2 3,404,164 + +0.0 3,404,165 - +0.4 3,404,174 + -0.1 3,404,174 + +0.2 3,404,174 + -0.2 3,404,175 - +0.5 3,404,175 - -0.1 3,404,181 + -0.1 3,404,181 + +1.4 3,404,182 - -0.0 3,404,182 - -0.8 3,404,182 - -1.6 3,404,763 - +1.1 3,404,784 - -0.3 3,404,785 + +0.5 3,404,785 + +0.3 3,404,785 + -0.6 3,404,785 + +1.1 3,404,785 + +0.5 3,404,785 + +1.2 3,404,785 + +0.3 3,404,786 - +0.3 3,404,786 - +0.5 3,404,786 - +0.0 3,404,786 - +0.4 3,404,786 - -0.4 3,404,993 - -1.0 3,405,085 - ABZR87_RS16110 0.15 +0.6 3,405,085 - ABZR87_RS16110 0.15 +1.0 3,405,191 + ABZR87_RS16110 0.22 +0.1 3,405,203 + ABZR87_RS16110 0.23 -0.3 3,405,203 + ABZR87_RS16110 0.23 +0.2 3,405,203 + ABZR87_RS16110 0.23 +0.4 3,405,256 + ABZR87_RS16110 0.27 -0.1 3,405,256 + ABZR87_RS16110 0.27 -0.2 3,405,257 - ABZR87_RS16110 0.27 +0.1 3,405,317 + ABZR87_RS16110 0.31 +0.7 3,405,479 + ABZR87_RS16110 0.42 +0.7 3,405,749 + ABZR87_RS16110 0.61 +0.8 3,405,749 + ABZR87_RS16110 0.61 +0.5 3,405,749 + ABZR87_RS16110 0.61 -1.8 3,405,749 + ABZR87_RS16110 0.61 +0.5 3,405,750 - ABZR87_RS16110 0.61 +0.0 3,405,750 - ABZR87_RS16110 0.61 -0.3 3,405,950 + ABZR87_RS16110 0.75 +0.1 3,405,950 + ABZR87_RS16110 0.75 -1.4 3,405,950 + ABZR87_RS16110 0.75 +0.3 3,405,950 + ABZR87_RS16110 0.75 +0.4 3,405,951 - ABZR87_RS16110 0.75 +0.1 3,405,951 - ABZR87_RS16110 0.75 -0.2 3,405,951 - ABZR87_RS16110 0.75 +1.5 3,405,951 - ABZR87_RS16110 0.75 -0.3 3,406,085 + ABZR87_RS16110 0.85 +0.5 3,406,086 - ABZR87_RS16110 0.85 +0.1 3,406,262 + -0.9 3,406,263 - -0.2 3,406,263 - +2.0 3,406,304 + +0.3 3,406,586 - ABZR87_RS16115 0.31 -0.6 3,406,595 + ABZR87_RS16115 0.33 -0.6 3,406,599 + ABZR87_RS16115 0.33 -1.0 3,406,599 + ABZR87_RS16115 0.33 +0.1 3,406,599 + ABZR87_RS16115 0.33 -0.2 3,406,599 + ABZR87_RS16115 0.33 -0.3 3,406,600 - ABZR87_RS16115 0.33 +0.0 3,406,600 - ABZR87_RS16115 0.33 +0.5 3,406,702 + ABZR87_RS16115 0.47 +0.6 3,406,702 + ABZR87_RS16115 0.47 -0.2 3,406,702 + ABZR87_RS16115 0.47 -0.3 3,406,703 - ABZR87_RS16115 0.47 -1.4 3,406,810 + ABZR87_RS16115 0.61 -0.0 3,406,811 - ABZR87_RS16115 0.61 -0.7 3,406,943 + ABZR87_RS16115 0.78 +0.3 3,407,035 + -1.1 3,407,036 - -0.8 3,407,036 - +0.2 3,407,036 - +0.4 3,407,036 - +0.8 3,407,102 + +0.6 3,407,102 + -0.9 3,407,102 + -0.5 3,407,103 - +1.2 3,407,103 - +0.4 3,407,103 - +0.6 3,407,105 + -0.3 3,407,105 - -0.0 3,407,105 - +0.5 3,407,179 + +0.1 3,407,179 + +0.4 3,407,180 - +0.1 3,407,183 + -0.3 3,407,183 + -1.0 3,407,183 + -0.9 3,407,213 + -0.2 3,407,214 - +0.2 3,407,214 - -0.5 3,407,214 - +0.2 3,407,214 - +0.1 3,407,273 + -1.9 3,407,273 + +0.4 3,407,273 + -0.3 3,407,273 + -0.2
Or see this region's nucleotide sequence