Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS16015 and ABZR87_RS16020 are separated by 52 nucleotides ABZR87_RS16020 and ABZR87_RS16025 are separated by 79 nucleotides ABZR87_RS16025 and ABZR87_RS16030 are separated by 78 nucleotides
ABZR87_RS16015: ABZR87_RS16015 - acyltransferase, at 3,386,148 to 3,387,098
_RS16015
ABZR87_RS16020: ABZR87_RS16020 - tRNA-Ser, at 3,387,151 to 3,387,240
_RS16020
ABZR87_RS16025: ABZR87_RS16025 - serine--tRNA ligase, at 3,387,320 to 3,388,636
_RS16025
ABZR87_RS16030: ABZR87_RS16030 - replication-associated recombination protein A, at 3,388,715 to 3,390,022
_RS16030
Position (kb)
3387
3388
3389 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3386.333 kb on + strand, within ABZR87_RS16015 at 3386.412 kb on + strand, within ABZR87_RS16015 at 3386.412 kb on + strand, within ABZR87_RS16015 at 3386.412 kb on + strand, within ABZR87_RS16015 at 3386.413 kb on - strand, within ABZR87_RS16015 at 3386.413 kb on - strand, within ABZR87_RS16015 at 3386.520 kb on + strand, within ABZR87_RS16015 at 3386.520 kb on + strand, within ABZR87_RS16015 at 3386.520 kb on + strand, within ABZR87_RS16015 at 3386.521 kb on - strand, within ABZR87_RS16015 at 3386.704 kb on - strand, within ABZR87_RS16015 at 3386.740 kb on - strand, within ABZR87_RS16015 at 3386.822 kb on + strand, within ABZR87_RS16015 at 3386.901 kb on - strand, within ABZR87_RS16015 at 3386.901 kb on - strand, within ABZR87_RS16015 at 3386.901 kb on - strand, within ABZR87_RS16015 at 3386.901 kb on - strand, within ABZR87_RS16015 at 3386.943 kb on + strand, within ABZR87_RS16015 at 3386.944 kb on - strand, within ABZR87_RS16015 at 3387.096 kb on + strand at 3387.097 kb on - strand at 3387.126 kb on - strand at 3387.145 kb on + strand at 3387.145 kb on + strand at 3388.695 kb on + strand at 3388.696 kb on - strand at 3388.716 kb on + strand at 3388.716 kb on + strand at 3388.716 kb on + strand at 3388.716 kb on + strand at 3388.716 kb on + strand at 3388.717 kb on - strand at 3388.717 kb on - strand at 3388.717 kb on - strand at 3388.724 kb on - strand at 3388.724 kb on - strand at 3388.828 kb on - strand at 3388.828 kb on - strand at 3388.860 kb on + strand, within ABZR87_RS16030 at 3388.860 kb on + strand, within ABZR87_RS16030 at 3388.860 kb on + strand, within ABZR87_RS16030 at 3388.878 kb on + strand, within ABZR87_RS16030 at 3388.878 kb on + strand, within ABZR87_RS16030 at 3388.878 kb on + strand, within ABZR87_RS16030 at 3388.878 kb on + strand, within ABZR87_RS16030 at 3388.879 kb on - strand, within ABZR87_RS16030 at 3388.903 kb on - strand, within ABZR87_RS16030 at 3388.908 kb on + strand, within ABZR87_RS16030 at 3389.016 kb on + strand, within ABZR87_RS16030 at 3389.016 kb on + strand, within ABZR87_RS16030 at 3389.025 kb on + strand, within ABZR87_RS16030 at 3389.025 kb on + strand, within ABZR87_RS16030 at 3389.025 kb on + strand, within ABZR87_RS16030 at 3389.026 kb on - strand, within ABZR87_RS16030 at 3389.026 kb on - strand, within ABZR87_RS16030 at 3389.026 kb on - strand, within ABZR87_RS16030 at 3389.058 kb on + strand, within ABZR87_RS16030 at 3389.058 kb on + strand, within ABZR87_RS16030 at 3389.059 kb on - strand, within ABZR87_RS16030 at 3389.059 kb on - strand, within ABZR87_RS16030 at 3389.059 kb on - strand, within ABZR87_RS16030 at 3389.059 kb on - strand, within ABZR87_RS16030 at 3389.112 kb on + strand, within ABZR87_RS16030 at 3389.113 kb on - strand, within ABZR87_RS16030 at 3389.205 kb on + strand, within ABZR87_RS16030 at 3389.247 kb on + strand, within ABZR87_RS16030 at 3389.248 kb on - strand, within ABZR87_RS16030 at 3389.307 kb on + strand, within ABZR87_RS16030 at 3389.307 kb on + strand, within ABZR87_RS16030 at 3389.307 kb on + strand, within ABZR87_RS16030 at 3389.308 kb on - strand, within ABZR87_RS16030 at 3389.308 kb on - strand, within ABZR87_RS16030 at 3389.584 kb on - strand, within ABZR87_RS16030
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 3,386,333 + ABZR87_RS16015 0.19 +0.3 3,386,412 + ABZR87_RS16015 0.28 +1.2 3,386,412 + ABZR87_RS16015 0.28 +0.6 3,386,412 + ABZR87_RS16015 0.28 -0.8 3,386,413 - ABZR87_RS16015 0.28 -0.3 3,386,413 - ABZR87_RS16015 0.28 -0.7 3,386,520 + ABZR87_RS16015 0.39 -0.5 3,386,520 + ABZR87_RS16015 0.39 -0.1 3,386,520 + ABZR87_RS16015 0.39 +0.0 3,386,521 - ABZR87_RS16015 0.39 -1.1 3,386,704 - ABZR87_RS16015 0.58 +0.6 3,386,740 - ABZR87_RS16015 0.62 -1.3 3,386,822 + ABZR87_RS16015 0.71 -0.3 3,386,901 - ABZR87_RS16015 0.79 -0.7 3,386,901 - ABZR87_RS16015 0.79 +0.9 3,386,901 - ABZR87_RS16015 0.79 +0.1 3,386,901 - ABZR87_RS16015 0.79 -0.5 3,386,943 + ABZR87_RS16015 0.84 -0.8 3,386,944 - ABZR87_RS16015 0.84 -0.6 3,387,096 + -0.9 3,387,097 - +0.2 3,387,126 - -0.4 3,387,145 + -0.2 3,387,145 + +0.7 3,388,695 + -0.6 3,388,696 - -0.4 3,388,716 + -0.2 3,388,716 + -2.0 3,388,716 + +2.4 3,388,716 + -0.0 3,388,716 + -0.1 3,388,717 - -0.1 3,388,717 - -0.2 3,388,717 - +0.5 3,388,724 - +0.9 3,388,724 - -0.3 3,388,828 - +0.7 3,388,828 - -2.1 3,388,860 + ABZR87_RS16030 0.11 -0.9 3,388,860 + ABZR87_RS16030 0.11 +0.4 3,388,860 + ABZR87_RS16030 0.11 -0.4 3,388,878 + ABZR87_RS16030 0.12 +0.1 3,388,878 + ABZR87_RS16030 0.12 +0.8 3,388,878 + ABZR87_RS16030 0.12 +2.4 3,388,878 + ABZR87_RS16030 0.12 +0.1 3,388,879 - ABZR87_RS16030 0.13 -0.6 3,388,903 - ABZR87_RS16030 0.14 -1.0 3,388,908 + ABZR87_RS16030 0.15 +0.4 3,389,016 + ABZR87_RS16030 0.23 +0.8 3,389,016 + ABZR87_RS16030 0.23 -0.9 3,389,025 + ABZR87_RS16030 0.24 +0.7 3,389,025 + ABZR87_RS16030 0.24 -0.4 3,389,025 + ABZR87_RS16030 0.24 -0.0 3,389,026 - ABZR87_RS16030 0.24 -0.4 3,389,026 - ABZR87_RS16030 0.24 -1.1 3,389,026 - ABZR87_RS16030 0.24 +0.7 3,389,058 + ABZR87_RS16030 0.26 -0.1 3,389,058 + ABZR87_RS16030 0.26 -0.7 3,389,059 - ABZR87_RS16030 0.26 +0.9 3,389,059 - ABZR87_RS16030 0.26 -0.3 3,389,059 - ABZR87_RS16030 0.26 +0.5 3,389,059 - ABZR87_RS16030 0.26 +1.4 3,389,112 + ABZR87_RS16030 0.30 -1.3 3,389,113 - ABZR87_RS16030 0.30 -0.3 3,389,205 + ABZR87_RS16030 0.37 -1.0 3,389,247 + ABZR87_RS16030 0.41 +0.4 3,389,248 - ABZR87_RS16030 0.41 +1.2 3,389,307 + ABZR87_RS16030 0.45 -0.1 3,389,307 + ABZR87_RS16030 0.45 +1.2 3,389,307 + ABZR87_RS16030 0.45 -0.0 3,389,308 - ABZR87_RS16030 0.45 +0.0 3,389,308 - ABZR87_RS16030 0.45 +0.1 3,389,584 - ABZR87_RS16030 0.66 +1.7
Or see this region's nucleotide sequence