Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS15765 and ABZR87_RS15770 overlap by 4 nucleotides ABZR87_RS15770 and ABZR87_RS15775 are separated by 84 nucleotides ABZR87_RS15775 and ABZR87_RS15780 overlap by 8 nucleotides
ABZR87_RS15765: ABZR87_RS15765 - branched-chain amino acid ABC transporter permease, at 3,328,736 to 3,329,770
_RS15765
ABZR87_RS15770: ABZR87_RS15770 - branched-chain amino acid ABC transporter permease, at 3,329,767 to 3,330,636
_RS15770
ABZR87_RS15775: ABZR87_RS15775 - 4-carboxymuconolactone decarboxylase, at 3,330,721 to 3,331,119
_RS15775
ABZR87_RS15780: ABZR87_RS15780 - 3-oxoadipate enol-lactonase, at 3,331,112 to 3,331,924
_RS15780
Position (kb)
3329
3330
3331 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3328.867 kb on - strand, within ABZR87_RS15765 at 3328.867 kb on - strand, within ABZR87_RS15765 at 3328.867 kb on - strand, within ABZR87_RS15765 at 3328.935 kb on + strand, within ABZR87_RS15765 at 3328.935 kb on + strand, within ABZR87_RS15765 at 3328.936 kb on - strand, within ABZR87_RS15765 at 3328.936 kb on - strand, within ABZR87_RS15765 at 3329.193 kb on - strand, within ABZR87_RS15765 at 3329.193 kb on - strand, within ABZR87_RS15765 at 3329.223 kb on + strand, within ABZR87_RS15765 at 3329.224 kb on - strand, within ABZR87_RS15765 at 3329.224 kb on - strand, within ABZR87_RS15765 at 3329.229 kb on + strand, within ABZR87_RS15765 at 3329.229 kb on + strand, within ABZR87_RS15765 at 3329.229 kb on + strand, within ABZR87_RS15765 at 3329.344 kb on - strand, within ABZR87_RS15765 at 3329.508 kb on + strand, within ABZR87_RS15765 at 3329.508 kb on + strand, within ABZR87_RS15765 at 3329.829 kb on - strand at 3329.858 kb on + strand, within ABZR87_RS15770 at 3329.858 kb on + strand, within ABZR87_RS15770 at 3329.858 kb on + strand, within ABZR87_RS15770 at 3329.859 kb on - strand, within ABZR87_RS15770 at 3329.859 kb on - strand, within ABZR87_RS15770 at 3329.859 kb on - strand, within ABZR87_RS15770 at 3329.859 kb on - strand, within ABZR87_RS15770 at 3329.861 kb on + strand, within ABZR87_RS15770 at 3329.862 kb on - strand, within ABZR87_RS15770 at 3329.862 kb on - strand, within ABZR87_RS15770 at 3329.957 kb on + strand, within ABZR87_RS15770 at 3329.958 kb on - strand, within ABZR87_RS15770 at 3329.958 kb on - strand, within ABZR87_RS15770 at 3329.966 kb on + strand, within ABZR87_RS15770 at 3329.966 kb on + strand, within ABZR87_RS15770 at 3329.966 kb on + strand, within ABZR87_RS15770 at 3329.966 kb on + strand, within ABZR87_RS15770 at 3329.966 kb on + strand, within ABZR87_RS15770 at 3329.967 kb on - strand, within ABZR87_RS15770 at 3329.967 kb on - strand, within ABZR87_RS15770 at 3329.967 kb on - strand, within ABZR87_RS15770 at 3329.967 kb on - strand, within ABZR87_RS15770 at 3330.268 kb on + strand, within ABZR87_RS15770 at 3330.303 kb on - strand, within ABZR87_RS15770 at 3330.374 kb on + strand, within ABZR87_RS15770 at 3330.374 kb on + strand, within ABZR87_RS15770 at 3330.375 kb on - strand, within ABZR87_RS15770 at 3330.494 kb on + strand, within ABZR87_RS15770 at 3330.495 kb on - strand, within ABZR87_RS15770 at 3330.597 kb on - strand at 3330.723 kb on - strand at 3330.809 kb on + strand, within ABZR87_RS15775 at 3330.809 kb on + strand, within ABZR87_RS15775 at 3330.809 kb on + strand, within ABZR87_RS15775 at 3330.990 kb on - strand, within ABZR87_RS15775 at 3331.101 kb on - strand at 3331.174 kb on - strand at 3331.224 kb on + strand, within ABZR87_RS15780 at 3331.224 kb on + strand, within ABZR87_RS15780 at 3331.224 kb on + strand, within ABZR87_RS15780 at 3331.484 kb on - strand, within ABZR87_RS15780 at 3331.526 kb on - strand, within ABZR87_RS15780
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 3,328,867 - ABZR87_RS15765 0.13 -0.1 3,328,867 - ABZR87_RS15765 0.13 -0.0 3,328,867 - ABZR87_RS15765 0.13 -0.2 3,328,935 + ABZR87_RS15765 0.19 -0.5 3,328,935 + ABZR87_RS15765 0.19 -0.2 3,328,936 - ABZR87_RS15765 0.19 -0.4 3,328,936 - ABZR87_RS15765 0.19 +0.4 3,329,193 - ABZR87_RS15765 0.44 +0.7 3,329,193 - ABZR87_RS15765 0.44 -2.2 3,329,223 + ABZR87_RS15765 0.47 -0.8 3,329,224 - ABZR87_RS15765 0.47 +0.5 3,329,224 - ABZR87_RS15765 0.47 -1.5 3,329,229 + ABZR87_RS15765 0.48 -0.7 3,329,229 + ABZR87_RS15765 0.48 +0.4 3,329,229 + ABZR87_RS15765 0.48 -0.4 3,329,344 - ABZR87_RS15765 0.59 -0.2 3,329,508 + ABZR87_RS15765 0.75 +3.0 3,329,508 + ABZR87_RS15765 0.75 -0.9 3,329,829 - +0.1 3,329,858 + ABZR87_RS15770 0.10 -0.0 3,329,858 + ABZR87_RS15770 0.10 -0.2 3,329,858 + ABZR87_RS15770 0.10 -0.5 3,329,859 - ABZR87_RS15770 0.11 -0.2 3,329,859 - ABZR87_RS15770 0.11 -0.4 3,329,859 - ABZR87_RS15770 0.11 -0.6 3,329,859 - ABZR87_RS15770 0.11 -0.1 3,329,861 + ABZR87_RS15770 0.11 +0.5 3,329,862 - ABZR87_RS15770 0.11 -0.2 3,329,862 - ABZR87_RS15770 0.11 -1.1 3,329,957 + ABZR87_RS15770 0.22 +0.2 3,329,958 - ABZR87_RS15770 0.22 -0.2 3,329,958 - ABZR87_RS15770 0.22 +0.3 3,329,966 + ABZR87_RS15770 0.23 +0.1 3,329,966 + ABZR87_RS15770 0.23 +0.2 3,329,966 + ABZR87_RS15770 0.23 -0.2 3,329,966 + ABZR87_RS15770 0.23 +0.1 3,329,966 + ABZR87_RS15770 0.23 +1.9 3,329,967 - ABZR87_RS15770 0.23 +0.3 3,329,967 - ABZR87_RS15770 0.23 -1.1 3,329,967 - ABZR87_RS15770 0.23 -0.9 3,329,967 - ABZR87_RS15770 0.23 -0.5 3,330,268 + ABZR87_RS15770 0.58 -0.9 3,330,303 - ABZR87_RS15770 0.62 +0.7 3,330,374 + ABZR87_RS15770 0.70 +1.0 3,330,374 + ABZR87_RS15770 0.70 -0.8 3,330,375 - ABZR87_RS15770 0.70 -0.8 3,330,494 + ABZR87_RS15770 0.84 -0.4 3,330,495 - ABZR87_RS15770 0.84 +1.3 3,330,597 - +0.4 3,330,723 - -0.4 3,330,809 + ABZR87_RS15775 0.22 +0.3 3,330,809 + ABZR87_RS15775 0.22 -0.1 3,330,809 + ABZR87_RS15775 0.22 +0.5 3,330,990 - ABZR87_RS15775 0.67 -0.7 3,331,101 - -0.1 3,331,174 - -0.0 3,331,224 + ABZR87_RS15780 0.14 -1.0 3,331,224 + ABZR87_RS15780 0.14 -0.2 3,331,224 + ABZR87_RS15780 0.14 -0.3 3,331,484 - ABZR87_RS15780 0.46 +0.2 3,331,526 - ABZR87_RS15780 0.51 -1.0
Or see this region's nucleotide sequence