Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS15520 and ABZR87_RS15525 are separated by 78 nucleotides ABZR87_RS15525 and ABZR87_RS15530 are separated by 106 nucleotides ABZR87_RS15530 and ABZR87_RS15535 are separated by 62 nucleotides
ABZR87_RS15520: ABZR87_RS15520 - flavodoxin family protein, at 3,268,560 to 3,269,132
_RS15520
ABZR87_RS15525: ABZR87_RS15525 - pirin family protein, at 3,269,211 to 3,269,909
_RS15525
ABZR87_RS15530: ABZR87_RS15530 - LysR substrate-binding domain-containing protein, at 3,270,016 to 3,270,918
_RS15530
ABZR87_RS15535: ABZR87_RS15535 - tRNA glutamyl-Q(34) synthetase GluQRS, at 3,270,981 to 3,271,916
_RS15535
Position (kb)
3270
3271 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3269.067 kb on + strand, within ABZR87_RS15520 at 3269.068 kb on - strand, within ABZR87_RS15520 at 3269.110 kb on + strand at 3269.110 kb on + strand at 3269.156 kb on + strand at 3269.350 kb on + strand, within ABZR87_RS15525 at 3269.350 kb on + strand, within ABZR87_RS15525 at 3269.350 kb on + strand, within ABZR87_RS15525 at 3269.350 kb on + strand, within ABZR87_RS15525 at 3269.350 kb on + strand, within ABZR87_RS15525 at 3269.351 kb on - strand, within ABZR87_RS15525 at 3269.351 kb on - strand, within ABZR87_RS15525 at 3269.351 kb on - strand, within ABZR87_RS15525 at 3269.351 kb on - strand, within ABZR87_RS15525 at 3269.351 kb on - strand, within ABZR87_RS15525 at 3269.351 kb on - strand, within ABZR87_RS15525 at 3269.413 kb on + strand, within ABZR87_RS15525 at 3269.413 kb on + strand, within ABZR87_RS15525 at 3269.710 kb on + strand, within ABZR87_RS15525 at 3269.710 kb on + strand, within ABZR87_RS15525 at 3269.710 kb on + strand, within ABZR87_RS15525 at 3269.710 kb on + strand, within ABZR87_RS15525 at 3269.710 kb on + strand, within ABZR87_RS15525 at 3269.711 kb on - strand, within ABZR87_RS15525 at 3269.711 kb on - strand, within ABZR87_RS15525 at 3269.711 kb on - strand, within ABZR87_RS15525 at 3269.711 kb on - strand, within ABZR87_RS15525 at 3269.723 kb on - strand, within ABZR87_RS15525 at 3269.824 kb on + strand, within ABZR87_RS15525 at 3269.824 kb on + strand, within ABZR87_RS15525 at 3269.824 kb on + strand, within ABZR87_RS15525 at 3269.824 kb on + strand, within ABZR87_RS15525 at 3269.825 kb on - strand, within ABZR87_RS15525 at 3269.825 kb on - strand, within ABZR87_RS15525 at 3269.825 kb on - strand, within ABZR87_RS15525 at 3269.825 kb on - strand, within ABZR87_RS15525 at 3269.848 kb on + strand at 3269.848 kb on + strand at 3269.848 kb on + strand at 3269.849 kb on - strand at 3269.849 kb on - strand at 3269.849 kb on - strand at 3269.849 kb on - strand at 3269.970 kb on + strand at 3269.970 kb on + strand at 3269.971 kb on - strand at 3269.981 kb on + strand at 3269.983 kb on + strand at 3269.984 kb on - strand at 3270.104 kb on + strand at 3270.104 kb on + strand at 3270.121 kb on + strand, within ABZR87_RS15530 at 3270.121 kb on + strand, within ABZR87_RS15530 at 3270.121 kb on + strand, within ABZR87_RS15530 at 3270.121 kb on + strand, within ABZR87_RS15530 at 3270.121 kb on + strand, within ABZR87_RS15530 at 3270.121 kb on + strand, within ABZR87_RS15530 at 3270.121 kb on + strand, within ABZR87_RS15530 at 3270.121 kb on + strand, within ABZR87_RS15530 at 3270.121 kb on + strand, within ABZR87_RS15530 at 3270.122 kb on - strand, within ABZR87_RS15530 at 3270.122 kb on - strand, within ABZR87_RS15530 at 3270.122 kb on - strand, within ABZR87_RS15530 at 3270.122 kb on - strand, within ABZR87_RS15530 at 3270.122 kb on - strand, within ABZR87_RS15530 at 3270.308 kb on + strand, within ABZR87_RS15530 at 3270.308 kb on + strand, within ABZR87_RS15530 at 3270.308 kb on + strand, within ABZR87_RS15530 at 3270.308 kb on + strand, within ABZR87_RS15530 at 3270.308 kb on + strand, within ABZR87_RS15530 at 3270.309 kb on - strand, within ABZR87_RS15530 at 3270.361 kb on + strand, within ABZR87_RS15530 at 3270.361 kb on + strand, within ABZR87_RS15530 at 3270.361 kb on + strand, within ABZR87_RS15530 at 3270.521 kb on + strand, within ABZR87_RS15530 at 3270.522 kb on - strand, within ABZR87_RS15530 at 3270.522 kb on - strand, within ABZR87_RS15530 at 3270.522 kb on - strand, within ABZR87_RS15530 at 3270.585 kb on + strand, within ABZR87_RS15530 at 3270.615 kb on + strand, within ABZR87_RS15530 at 3270.616 kb on - strand, within ABZR87_RS15530 at 3270.616 kb on - strand, within ABZR87_RS15530 at 3270.757 kb on + strand, within ABZR87_RS15530 at 3270.758 kb on - strand, within ABZR87_RS15530 at 3270.758 kb on - strand, within ABZR87_RS15530 at 3270.758 kb on - strand, within ABZR87_RS15530 at 3270.758 kb on - strand, within ABZR87_RS15530 at 3270.758 kb on - strand, within ABZR87_RS15530 at 3270.916 kb on + strand at 3270.947 kb on + strand at 3270.947 kb on + strand at 3270.947 kb on + strand at 3270.948 kb on - strand at 3271.036 kb on - strand at 3271.077 kb on - strand, within ABZR87_RS15535 at 3271.123 kb on + strand, within ABZR87_RS15535 at 3271.123 kb on + strand, within ABZR87_RS15535 at 3271.123 kb on + strand, within ABZR87_RS15535 at 3271.160 kb on - strand, within ABZR87_RS15535 at 3271.550 kb on - strand, within ABZR87_RS15535 at 3271.581 kb on + strand, within ABZR87_RS15535 at 3271.582 kb on - strand, within ABZR87_RS15535 at 3271.582 kb on - strand, within ABZR87_RS15535 at 3271.674 kb on + strand, within ABZR87_RS15535 at 3271.674 kb on + strand, within ABZR87_RS15535 at 3271.674 kb on + strand, within ABZR87_RS15535 at 3271.674 kb on + strand, within ABZR87_RS15535 at 3271.711 kb on - strand, within ABZR87_RS15535 at 3271.711 kb on - strand, within ABZR87_RS15535 at 3271.866 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 3,269,067 + ABZR87_RS15520 0.88 -0.1 3,269,068 - ABZR87_RS15520 0.89 -0.4 3,269,110 + +0.2 3,269,110 + -1.3 3,269,156 + +0.4 3,269,350 + ABZR87_RS15525 0.20 -2.3 3,269,350 + ABZR87_RS15525 0.20 +0.5 3,269,350 + ABZR87_RS15525 0.20 -0.7 3,269,350 + ABZR87_RS15525 0.20 +0.2 3,269,350 + ABZR87_RS15525 0.20 -1.0 3,269,351 - ABZR87_RS15525 0.20 +0.5 3,269,351 - ABZR87_RS15525 0.20 -0.7 3,269,351 - ABZR87_RS15525 0.20 -1.1 3,269,351 - ABZR87_RS15525 0.20 +0.7 3,269,351 - ABZR87_RS15525 0.20 -1.2 3,269,351 - ABZR87_RS15525 0.20 +0.2 3,269,413 + ABZR87_RS15525 0.29 -0.7 3,269,413 + ABZR87_RS15525 0.29 -1.0 3,269,710 + ABZR87_RS15525 0.71 +0.1 3,269,710 + ABZR87_RS15525 0.71 -1.0 3,269,710 + ABZR87_RS15525 0.71 -0.7 3,269,710 + ABZR87_RS15525 0.71 +0.2 3,269,710 + ABZR87_RS15525 0.71 +0.6 3,269,711 - ABZR87_RS15525 0.72 +0.6 3,269,711 - ABZR87_RS15525 0.72 -1.8 3,269,711 - ABZR87_RS15525 0.72 -0.1 3,269,711 - ABZR87_RS15525 0.72 +0.2 3,269,723 - ABZR87_RS15525 0.73 +0.3 3,269,824 + ABZR87_RS15525 0.88 -0.6 3,269,824 + ABZR87_RS15525 0.88 -0.3 3,269,824 + ABZR87_RS15525 0.88 +1.1 3,269,824 + ABZR87_RS15525 0.88 -0.6 3,269,825 - ABZR87_RS15525 0.88 -0.0 3,269,825 - ABZR87_RS15525 0.88 -0.4 3,269,825 - ABZR87_RS15525 0.88 -1.6 3,269,825 - ABZR87_RS15525 0.88 -0.7 3,269,848 + -1.6 3,269,848 + +0.1 3,269,848 + -0.6 3,269,849 - +0.0 3,269,849 - +0.2 3,269,849 - -2.4 3,269,849 - -0.1 3,269,970 + +2.0 3,269,970 + -0.7 3,269,971 - -0.5 3,269,981 + -1.0 3,269,983 + +0.5 3,269,984 - +0.3 3,270,104 + +0.2 3,270,104 + -0.6 3,270,121 + ABZR87_RS15530 0.12 -0.1 3,270,121 + ABZR87_RS15530 0.12 +0.4 3,270,121 + ABZR87_RS15530 0.12 +0.8 3,270,121 + ABZR87_RS15530 0.12 +0.2 3,270,121 + ABZR87_RS15530 0.12 +0.6 3,270,121 + ABZR87_RS15530 0.12 -1.8 3,270,121 + ABZR87_RS15530 0.12 -0.1 3,270,121 + ABZR87_RS15530 0.12 +0.6 3,270,121 + ABZR87_RS15530 0.12 -0.2 3,270,122 - ABZR87_RS15530 0.12 -1.3 3,270,122 - ABZR87_RS15530 0.12 +0.5 3,270,122 - ABZR87_RS15530 0.12 -0.0 3,270,122 - ABZR87_RS15530 0.12 +1.0 3,270,122 - ABZR87_RS15530 0.12 +0.3 3,270,308 + ABZR87_RS15530 0.32 +1.0 3,270,308 + ABZR87_RS15530 0.32 -0.4 3,270,308 + ABZR87_RS15530 0.32 +1.0 3,270,308 + ABZR87_RS15530 0.32 +0.3 3,270,308 + ABZR87_RS15530 0.32 +0.3 3,270,309 - ABZR87_RS15530 0.32 +0.0 3,270,361 + ABZR87_RS15530 0.38 -0.8 3,270,361 + ABZR87_RS15530 0.38 +1.3 3,270,361 + ABZR87_RS15530 0.38 -0.2 3,270,521 + ABZR87_RS15530 0.56 -0.1 3,270,522 - ABZR87_RS15530 0.56 -0.5 3,270,522 - ABZR87_RS15530 0.56 +0.1 3,270,522 - ABZR87_RS15530 0.56 -0.0 3,270,585 + ABZR87_RS15530 0.63 -0.1 3,270,615 + ABZR87_RS15530 0.66 +0.4 3,270,616 - ABZR87_RS15530 0.66 +1.0 3,270,616 - ABZR87_RS15530 0.66 +1.3 3,270,757 + ABZR87_RS15530 0.82 +1.1 3,270,758 - ABZR87_RS15530 0.82 +0.2 3,270,758 - ABZR87_RS15530 0.82 +0.6 3,270,758 - ABZR87_RS15530 0.82 -0.1 3,270,758 - ABZR87_RS15530 0.82 -0.3 3,270,758 - ABZR87_RS15530 0.82 -0.1 3,270,916 + -0.8 3,270,947 + -0.7 3,270,947 + -0.4 3,270,947 + -0.2 3,270,948 - +0.6 3,271,036 - -0.3 3,271,077 - ABZR87_RS15535 0.10 +0.3 3,271,123 + ABZR87_RS15535 0.15 +0.0 3,271,123 + ABZR87_RS15535 0.15 -1.2 3,271,123 + ABZR87_RS15535 0.15 +0.4 3,271,160 - ABZR87_RS15535 0.19 +1.1 3,271,550 - ABZR87_RS15535 0.61 +0.1 3,271,581 + ABZR87_RS15535 0.64 -1.0 3,271,582 - ABZR87_RS15535 0.64 +0.2 3,271,582 - ABZR87_RS15535 0.64 -1.6 3,271,674 + ABZR87_RS15535 0.74 -1.2 3,271,674 + ABZR87_RS15535 0.74 -0.5 3,271,674 + ABZR87_RS15535 0.74 -1.2 3,271,674 + ABZR87_RS15535 0.74 -0.2 3,271,711 - ABZR87_RS15535 0.78 -0.3 3,271,711 - ABZR87_RS15535 0.78 -1.9 3,271,866 + -0.0
Or see this region's nucleotide sequence