Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS14965 and ABZR87_RS14970 are separated by 11 nucleotides ABZR87_RS14970 and ABZR87_RS14975 are separated by 96 nucleotides ABZR87_RS14975 and ABZR87_RS14980 are separated by 30 nucleotides
ABZR87_RS14965: ABZR87_RS14965 - DUF3619 family protein, at 3,157,342 to 3,157,782
_RS14965
ABZR87_RS14970: ABZR87_RS14970 - DUF3106 domain-containing protein, at 3,157,794 to 3,158,639
_RS14970
ABZR87_RS14975: ABZR87_RS14975 - RDD family protein, at 3,158,736 to 3,159,260
_RS14975
ABZR87_RS14980: ABZR87_RS14980 - alpha/beta fold hydrolase, at 3,159,291 to 3,160,307
_RS14980
Position (kb)
3158
3159
3160 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3157.762 kb on + strand at 3157.763 kb on - strand at 3157.942 kb on + strand, within ABZR87_RS14970 at 3157.942 kb on + strand, within ABZR87_RS14970 at 3157.942 kb on + strand, within ABZR87_RS14970 at 3158.142 kb on + strand, within ABZR87_RS14970 at 3158.142 kb on + strand, within ABZR87_RS14970 at 3158.286 kb on + strand, within ABZR87_RS14970 at 3158.286 kb on + strand, within ABZR87_RS14970 at 3158.287 kb on - strand, within ABZR87_RS14970 at 3158.426 kb on + strand, within ABZR87_RS14970 at 3158.426 kb on + strand, within ABZR87_RS14970 at 3158.661 kb on - strand at 3158.826 kb on + strand, within ABZR87_RS14975 at 3158.826 kb on + strand, within ABZR87_RS14975 at 3158.827 kb on - strand, within ABZR87_RS14975 at 3158.827 kb on - strand, within ABZR87_RS14975 at 3158.827 kb on - strand, within ABZR87_RS14975 at 3158.935 kb on + strand, within ABZR87_RS14975 at 3158.935 kb on + strand, within ABZR87_RS14975 at 3158.936 kb on - strand, within ABZR87_RS14975 at 3158.936 kb on - strand, within ABZR87_RS14975 at 3158.961 kb on + strand, within ABZR87_RS14975 at 3158.961 kb on + strand, within ABZR87_RS14975 at 3158.962 kb on - strand, within ABZR87_RS14975 at 3158.962 kb on - strand, within ABZR87_RS14975 at 3158.962 kb on - strand, within ABZR87_RS14975 at 3158.962 kb on - strand, within ABZR87_RS14975 at 3159.047 kb on + strand, within ABZR87_RS14975 at 3159.076 kb on - strand, within ABZR87_RS14975 at 3159.115 kb on + strand, within ABZR87_RS14975 at 3159.116 kb on - strand, within ABZR87_RS14975 at 3159.116 kb on - strand, within ABZR87_RS14975 at 3159.735 kb on + strand, within ABZR87_RS14980 at 3159.735 kb on + strand, within ABZR87_RS14980 at 3159.736 kb on - strand, within ABZR87_RS14980 at 3159.843 kb on + strand, within ABZR87_RS14980 at 3159.880 kb on + strand, within ABZR87_RS14980 at 3159.881 kb on - strand, within ABZR87_RS14980 at 3160.228 kb on + strand at 3160.229 kb on - strand at 3160.229 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 3,157,762 + -0.2 3,157,763 - +0.6 3,157,942 + ABZR87_RS14970 0.17 -1.4 3,157,942 + ABZR87_RS14970 0.17 -1.2 3,157,942 + ABZR87_RS14970 0.17 +0.1 3,158,142 + ABZR87_RS14970 0.41 -1.3 3,158,142 + ABZR87_RS14970 0.41 -0.3 3,158,286 + ABZR87_RS14970 0.58 +1.8 3,158,286 + ABZR87_RS14970 0.58 -0.3 3,158,287 - ABZR87_RS14970 0.58 -0.8 3,158,426 + ABZR87_RS14970 0.75 -0.4 3,158,426 + ABZR87_RS14970 0.75 -1.3 3,158,661 - +0.3 3,158,826 + ABZR87_RS14975 0.17 -1.1 3,158,826 + ABZR87_RS14975 0.17 -1.6 3,158,827 - ABZR87_RS14975 0.17 -0.3 3,158,827 - ABZR87_RS14975 0.17 -1.3 3,158,827 - ABZR87_RS14975 0.17 -1.7 3,158,935 + ABZR87_RS14975 0.38 -0.7 3,158,935 + ABZR87_RS14975 0.38 -0.9 3,158,936 - ABZR87_RS14975 0.38 +0.1 3,158,936 - ABZR87_RS14975 0.38 +1.4 3,158,961 + ABZR87_RS14975 0.43 -0.2 3,158,961 + ABZR87_RS14975 0.43 +1.2 3,158,962 - ABZR87_RS14975 0.43 -0.1 3,158,962 - ABZR87_RS14975 0.43 +0.5 3,158,962 - ABZR87_RS14975 0.43 +0.0 3,158,962 - ABZR87_RS14975 0.43 -1.5 3,159,047 + ABZR87_RS14975 0.59 -0.6 3,159,076 - ABZR87_RS14975 0.65 +0.5 3,159,115 + ABZR87_RS14975 0.72 -0.7 3,159,116 - ABZR87_RS14975 0.72 -1.1 3,159,116 - ABZR87_RS14975 0.72 +0.7 3,159,735 + ABZR87_RS14980 0.44 +0.2 3,159,735 + ABZR87_RS14980 0.44 -2.2 3,159,736 - ABZR87_RS14980 0.44 +0.1 3,159,843 + ABZR87_RS14980 0.54 +0.3 3,159,880 + ABZR87_RS14980 0.58 -0.1 3,159,881 - ABZR87_RS14980 0.58 -0.7 3,160,228 + +0.7 3,160,229 - +0.4 3,160,229 - -0.5
Or see this region's nucleotide sequence