Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS14670 and ABZR87_RS14675 are separated by 9 nucleotides ABZR87_RS14675 and ABZR87_RS14680 overlap by 4 nucleotides ABZR87_RS14680 and ABZR87_RS14685 are separated by 119 nucleotides
ABZR87_RS14670: ABZR87_RS14670 - phosphotransferase, at 3,098,843 to 3,099,931
_RS14670
ABZR87_RS14675: ABZR87_RS14675 - glyoxalase, at 3,099,941 to 3,100,360
_RS14675
ABZR87_RS14680: ABZR87_RS14680 - SDR family oxidoreductase, at 3,100,357 to 3,101,070
_RS14680
ABZR87_RS14685: ABZR87_RS14685 - LysR family transcriptional regulator, at 3,101,190 to 3,102,176
_RS14685
Position (kb)
3099
3100
3101 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3099.041 kb on + strand, within ABZR87_RS14670 at 3099.041 kb on + strand, within ABZR87_RS14670 at 3099.041 kb on + strand, within ABZR87_RS14670 at 3099.041 kb on + strand, within ABZR87_RS14670 at 3099.041 kb on + strand, within ABZR87_RS14670 at 3099.042 kb on - strand, within ABZR87_RS14670 at 3099.042 kb on - strand, within ABZR87_RS14670 at 3099.161 kb on + strand, within ABZR87_RS14670 at 3099.161 kb on + strand, within ABZR87_RS14670 at 3099.161 kb on + strand, within ABZR87_RS14670 at 3099.161 kb on + strand, within ABZR87_RS14670 at 3099.161 kb on + strand, within ABZR87_RS14670 at 3099.161 kb on + strand, within ABZR87_RS14670 at 3099.162 kb on - strand, within ABZR87_RS14670 at 3099.162 kb on - strand, within ABZR87_RS14670 at 3099.190 kb on - strand, within ABZR87_RS14670 at 3099.203 kb on + strand, within ABZR87_RS14670 at 3099.203 kb on + strand, within ABZR87_RS14670 at 3099.203 kb on + strand, within ABZR87_RS14670 at 3099.203 kb on + strand, within ABZR87_RS14670 at 3099.203 kb on + strand, within ABZR87_RS14670 at 3099.204 kb on - strand, within ABZR87_RS14670 at 3099.204 kb on - strand, within ABZR87_RS14670 at 3099.204 kb on - strand, within ABZR87_RS14670 at 3099.204 kb on - strand, within ABZR87_RS14670 at 3099.204 kb on - strand, within ABZR87_RS14670 at 3099.204 kb on - strand, within ABZR87_RS14670 at 3099.220 kb on - strand, within ABZR87_RS14670 at 3099.220 kb on - strand, within ABZR87_RS14670 at 3099.284 kb on + strand, within ABZR87_RS14670 at 3099.285 kb on - strand, within ABZR87_RS14670 at 3099.285 kb on - strand, within ABZR87_RS14670 at 3099.285 kb on - strand, within ABZR87_RS14670 at 3099.285 kb on - strand, within ABZR87_RS14670 at 3099.329 kb on + strand, within ABZR87_RS14670 at 3099.354 kb on - strand, within ABZR87_RS14670 at 3099.371 kb on + strand, within ABZR87_RS14670 at 3099.372 kb on - strand, within ABZR87_RS14670 at 3099.372 kb on - strand, within ABZR87_RS14670 at 3099.372 kb on - strand, within ABZR87_RS14670 at 3099.408 kb on - strand, within ABZR87_RS14670 at 3099.408 kb on - strand, within ABZR87_RS14670 at 3099.408 kb on - strand, within ABZR87_RS14670 at 3099.408 kb on - strand, within ABZR87_RS14670 at 3099.621 kb on - strand, within ABZR87_RS14670 at 3099.713 kb on + strand, within ABZR87_RS14670 at 3099.713 kb on + strand, within ABZR87_RS14670 at 3099.714 kb on - strand, within ABZR87_RS14670 at 3099.714 kb on - strand, within ABZR87_RS14670 at 3099.770 kb on + strand, within ABZR87_RS14670 at 3099.771 kb on - strand, within ABZR87_RS14670 at 3099.771 kb on - strand, within ABZR87_RS14670 at 3099.779 kb on + strand, within ABZR87_RS14670 at 3099.780 kb on - strand, within ABZR87_RS14670 at 3099.780 kb on - strand, within ABZR87_RS14670 at 3099.909 kb on - strand at 3099.963 kb on + strand at 3099.964 kb on - strand at 3100.148 kb on - strand, within ABZR87_RS14675 at 3100.360 kb on + strand at 3100.360 kb on + strand at 3100.361 kb on - strand at 3100.361 kb on - strand at 3100.410 kb on + strand at 3100.410 kb on + strand at 3100.706 kb on + strand, within ABZR87_RS14680 at 3100.899 kb on + strand, within ABZR87_RS14680 at 3101.148 kb on - strand at 3101.148 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 3,099,041 + ABZR87_RS14670 0.18 +0.5 3,099,041 + ABZR87_RS14670 0.18 +0.4 3,099,041 + ABZR87_RS14670 0.18 -0.0 3,099,041 + ABZR87_RS14670 0.18 -1.3 3,099,041 + ABZR87_RS14670 0.18 +0.0 3,099,042 - ABZR87_RS14670 0.18 -0.2 3,099,042 - ABZR87_RS14670 0.18 +0.1 3,099,161 + ABZR87_RS14670 0.29 +0.4 3,099,161 + ABZR87_RS14670 0.29 +0.0 3,099,161 + ABZR87_RS14670 0.29 -2.0 3,099,161 + ABZR87_RS14670 0.29 -0.0 3,099,161 + ABZR87_RS14670 0.29 -0.1 3,099,161 + ABZR87_RS14670 0.29 +0.2 3,099,162 - ABZR87_RS14670 0.29 +0.2 3,099,162 - ABZR87_RS14670 0.29 -0.1 3,099,190 - ABZR87_RS14670 0.32 -0.1 3,099,203 + ABZR87_RS14670 0.33 -0.0 3,099,203 + ABZR87_RS14670 0.33 +0.2 3,099,203 + ABZR87_RS14670 0.33 -0.8 3,099,203 + ABZR87_RS14670 0.33 +0.3 3,099,203 + ABZR87_RS14670 0.33 +0.3 3,099,204 - ABZR87_RS14670 0.33 +0.7 3,099,204 - ABZR87_RS14670 0.33 -0.3 3,099,204 - ABZR87_RS14670 0.33 -0.2 3,099,204 - ABZR87_RS14670 0.33 -3.0 3,099,204 - ABZR87_RS14670 0.33 +0.8 3,099,204 - ABZR87_RS14670 0.33 -0.3 3,099,220 - ABZR87_RS14670 0.35 +0.0 3,099,220 - ABZR87_RS14670 0.35 +0.5 3,099,284 + ABZR87_RS14670 0.40 +0.8 3,099,285 - ABZR87_RS14670 0.41 +1.0 3,099,285 - ABZR87_RS14670 0.41 -0.1 3,099,285 - ABZR87_RS14670 0.41 +0.6 3,099,285 - ABZR87_RS14670 0.41 +1.0 3,099,329 + ABZR87_RS14670 0.45 +0.0 3,099,354 - ABZR87_RS14670 0.47 +2.0 3,099,371 + ABZR87_RS14670 0.48 -0.4 3,099,372 - ABZR87_RS14670 0.49 +0.3 3,099,372 - ABZR87_RS14670 0.49 +0.0 3,099,372 - ABZR87_RS14670 0.49 -0.7 3,099,408 - ABZR87_RS14670 0.52 +0.1 3,099,408 - ABZR87_RS14670 0.52 -0.3 3,099,408 - ABZR87_RS14670 0.52 -0.6 3,099,408 - ABZR87_RS14670 0.52 +0.6 3,099,621 - ABZR87_RS14670 0.71 -0.4 3,099,713 + ABZR87_RS14670 0.80 -0.1 3,099,713 + ABZR87_RS14670 0.80 -0.5 3,099,714 - ABZR87_RS14670 0.80 -0.4 3,099,714 - ABZR87_RS14670 0.80 -0.2 3,099,770 + ABZR87_RS14670 0.85 -1.4 3,099,771 - ABZR87_RS14670 0.85 -1.1 3,099,771 - ABZR87_RS14670 0.85 -1.2 3,099,779 + ABZR87_RS14670 0.86 +0.1 3,099,780 - ABZR87_RS14670 0.86 +0.2 3,099,780 - ABZR87_RS14670 0.86 +0.9 3,099,909 - -1.3 3,099,963 + -0.8 3,099,964 - -1.0 3,100,148 - ABZR87_RS14675 0.49 -1.3 3,100,360 + -0.6 3,100,360 + +0.8 3,100,361 - +0.4 3,100,361 - -0.8 3,100,410 + +0.9 3,100,410 + -0.3 3,100,706 + ABZR87_RS14680 0.49 +0.7 3,100,899 + ABZR87_RS14680 0.76 -0.3 3,101,148 - +0.7 3,101,148 - -0.3
Or see this region's nucleotide sequence