Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS14500 and ABZR87_RS14505 are separated by 95 nucleotides ABZR87_RS14505 and ABZR87_RS14510 are separated by 14 nucleotides ABZR87_RS14510 and ABZR87_RS14515 are separated by 83 nucleotides
ABZR87_RS14500: ABZR87_RS14500 - tryptophan synthase subunit beta, at 3,062,062 to 3,063,255
_RS14500
ABZR87_RS14505: ABZR87_RS14505 - phosphoribosylanthranilate isomerase, at 3,063,351 to 3,064,052
_RS14505
ABZR87_RS14510: ABZR87_RS14510 - tRNA pseudouridine(38-40) synthase TruA, at 3,064,067 to 3,064,891
_RS14510
ABZR87_RS14515: ABZR87_RS14515 - FimV/HubP family polar landmark-like protein TapV, at 3,064,975 to 3,067,890
_RS14515
Position (kb)
3063
3064
3065 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3062.393 kb on + strand, within ABZR87_RS14500 at 3062.393 kb on + strand, within ABZR87_RS14500 at 3062.393 kb on + strand, within ABZR87_RS14500 at 3062.394 kb on - strand, within ABZR87_RS14500 at 3062.394 kb on - strand, within ABZR87_RS14500 at 3062.394 kb on - strand, within ABZR87_RS14500 at 3062.394 kb on - strand, within ABZR87_RS14500 at 3062.516 kb on + strand, within ABZR87_RS14500 at 3062.516 kb on + strand, within ABZR87_RS14500 at 3062.516 kb on + strand, within ABZR87_RS14500 at 3062.516 kb on + strand, within ABZR87_RS14500 at 3062.522 kb on + strand, within ABZR87_RS14500 at 3062.523 kb on - strand, within ABZR87_RS14500 at 3062.657 kb on + strand, within ABZR87_RS14500 at 3062.690 kb on + strand, within ABZR87_RS14500 at 3062.690 kb on + strand, within ABZR87_RS14500 at 3062.690 kb on + strand, within ABZR87_RS14500 at 3062.691 kb on - strand, within ABZR87_RS14500 at 3062.691 kb on - strand, within ABZR87_RS14500 at 3062.691 kb on - strand, within ABZR87_RS14500 at 3062.691 kb on - strand, within ABZR87_RS14500 at 3062.691 kb on - strand, within ABZR87_RS14500 at 3062.691 kb on - strand, within ABZR87_RS14500 at 3062.691 kb on - strand, within ABZR87_RS14500 at 3062.691 kb on - strand, within ABZR87_RS14500 at 3062.691 kb on - strand, within ABZR87_RS14500 at 3062.691 kb on - strand, within ABZR87_RS14500 at 3062.849 kb on + strand, within ABZR87_RS14500 at 3062.849 kb on + strand, within ABZR87_RS14500 at 3062.850 kb on - strand, within ABZR87_RS14500 at 3062.850 kb on - strand, within ABZR87_RS14500 at 3062.850 kb on - strand, within ABZR87_RS14500 at 3062.850 kb on - strand, within ABZR87_RS14500 at 3062.871 kb on - strand, within ABZR87_RS14500 at 3063.026 kb on + strand, within ABZR87_RS14500 at 3063.026 kb on + strand, within ABZR87_RS14500 at 3063.026 kb on + strand, within ABZR87_RS14500 at 3063.026 kb on + strand, within ABZR87_RS14500 at 3063.026 kb on + strand, within ABZR87_RS14500 at 3063.026 kb on + strand, within ABZR87_RS14500 at 3063.027 kb on - strand, within ABZR87_RS14500 at 3063.027 kb on - strand, within ABZR87_RS14500 at 3063.027 kb on - strand, within ABZR87_RS14500 at 3063.027 kb on - strand, within ABZR87_RS14500 at 3063.071 kb on + strand, within ABZR87_RS14500 at 3063.072 kb on - strand, within ABZR87_RS14500 at 3063.072 kb on - strand, within ABZR87_RS14500 at 3063.111 kb on - strand, within ABZR87_RS14500 at 3063.111 kb on - strand, within ABZR87_RS14500 at 3063.116 kb on + strand, within ABZR87_RS14500 at 3063.155 kb on + strand at 3063.155 kb on + strand at 3063.156 kb on - strand at 3063.156 kb on - strand at 3063.251 kb on + strand at 3063.251 kb on + strand at 3063.251 kb on + strand at 3063.251 kb on + strand at 3063.251 kb on + strand at 3063.251 kb on + strand at 3063.252 kb on - strand at 3063.252 kb on - strand at 3063.252 kb on - strand at 3063.252 kb on - strand at 3063.252 kb on - strand at 3063.252 kb on - strand at 3063.309 kb on + strand at 3063.310 kb on - strand at 3063.310 kb on - strand at 3063.310 kb on - strand at 3063.475 kb on + strand, within ABZR87_RS14505 at 3063.476 kb on - strand, within ABZR87_RS14505 at 3063.635 kb on - strand, within ABZR87_RS14505 at 3063.635 kb on - strand, within ABZR87_RS14505 at 3063.931 kb on + strand, within ABZR87_RS14505 at 3063.950 kb on - strand, within ABZR87_RS14505 at 3063.950 kb on - strand, within ABZR87_RS14505 at 3063.950 kb on - strand, within ABZR87_RS14505 at 3064.038 kb on - strand at 3064.167 kb on + strand, within ABZR87_RS14510 at 3064.167 kb on + strand, within ABZR87_RS14510 at 3064.167 kb on + strand, within ABZR87_RS14510 at 3064.168 kb on - strand, within ABZR87_RS14510 at 3064.369 kb on - strand, within ABZR87_RS14510 at 3064.369 kb on - strand, within ABZR87_RS14510 at 3064.369 kb on - strand, within ABZR87_RS14510 at 3064.369 kb on - strand, within ABZR87_RS14510 at 3064.557 kb on + strand, within ABZR87_RS14510 at 3064.557 kb on + strand, within ABZR87_RS14510 at 3064.558 kb on - strand, within ABZR87_RS14510 at 3064.558 kb on - strand, within ABZR87_RS14510 at 3064.560 kb on + strand, within ABZR87_RS14510 at 3064.560 kb on + strand, within ABZR87_RS14510 at 3064.561 kb on - strand, within ABZR87_RS14510 at 3064.561 kb on - strand, within ABZR87_RS14510 at 3064.563 kb on + strand, within ABZR87_RS14510 at 3064.564 kb on - strand, within ABZR87_RS14510
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 3,062,393 + ABZR87_RS14500 0.28 -1.5 3,062,393 + ABZR87_RS14500 0.28 -0.8 3,062,393 + ABZR87_RS14500 0.28 -1.8 3,062,394 - ABZR87_RS14500 0.28 -1.3 3,062,394 - ABZR87_RS14500 0.28 -0.7 3,062,394 - ABZR87_RS14500 0.28 -0.1 3,062,394 - ABZR87_RS14500 0.28 -0.9 3,062,516 + ABZR87_RS14500 0.38 -0.7 3,062,516 + ABZR87_RS14500 0.38 -0.9 3,062,516 + ABZR87_RS14500 0.38 -2.3 3,062,516 + ABZR87_RS14500 0.38 -0.8 3,062,522 + ABZR87_RS14500 0.39 -1.5 3,062,523 - ABZR87_RS14500 0.39 -1.6 3,062,657 + ABZR87_RS14500 0.50 +0.6 3,062,690 + ABZR87_RS14500 0.53 -0.8 3,062,690 + ABZR87_RS14500 0.53 -0.8 3,062,690 + ABZR87_RS14500 0.53 -0.3 3,062,691 - ABZR87_RS14500 0.53 -1.4 3,062,691 - ABZR87_RS14500 0.53 -0.4 3,062,691 - ABZR87_RS14500 0.53 -1.5 3,062,691 - ABZR87_RS14500 0.53 +0.1 3,062,691 - ABZR87_RS14500 0.53 -0.8 3,062,691 - ABZR87_RS14500 0.53 -0.9 3,062,691 - ABZR87_RS14500 0.53 +0.3 3,062,691 - ABZR87_RS14500 0.53 -1.3 3,062,691 - ABZR87_RS14500 0.53 -1.4 3,062,691 - ABZR87_RS14500 0.53 -0.5 3,062,849 + ABZR87_RS14500 0.66 -0.6 3,062,849 + ABZR87_RS14500 0.66 +0.9 3,062,850 - ABZR87_RS14500 0.66 +1.4 3,062,850 - ABZR87_RS14500 0.66 -1.0 3,062,850 - ABZR87_RS14500 0.66 -2.2 3,062,850 - ABZR87_RS14500 0.66 -0.3 3,062,871 - ABZR87_RS14500 0.68 -0.5 3,063,026 + ABZR87_RS14500 0.81 +0.7 3,063,026 + ABZR87_RS14500 0.81 +0.4 3,063,026 + ABZR87_RS14500 0.81 -0.1 3,063,026 + ABZR87_RS14500 0.81 +0.2 3,063,026 + ABZR87_RS14500 0.81 -0.7 3,063,026 + ABZR87_RS14500 0.81 +0.0 3,063,027 - ABZR87_RS14500 0.81 +0.0 3,063,027 - ABZR87_RS14500 0.81 +1.4 3,063,027 - ABZR87_RS14500 0.81 -1.7 3,063,027 - ABZR87_RS14500 0.81 -0.3 3,063,071 + ABZR87_RS14500 0.85 -0.1 3,063,072 - ABZR87_RS14500 0.85 -2.3 3,063,072 - ABZR87_RS14500 0.85 -0.4 3,063,111 - ABZR87_RS14500 0.88 -1.5 3,063,111 - ABZR87_RS14500 0.88 -1.8 3,063,116 + ABZR87_RS14500 0.88 -0.5 3,063,155 + -1.0 3,063,155 + -2.0 3,063,156 - -0.5 3,063,156 - -0.8 3,063,251 + +0.2 3,063,251 + -0.4 3,063,251 + -0.9 3,063,251 + -0.1 3,063,251 + -0.4 3,063,251 + -1.0 3,063,252 - -0.9 3,063,252 - -0.8 3,063,252 - -0.9 3,063,252 - +0.5 3,063,252 - -0.8 3,063,252 - -0.5 3,063,309 + -0.1 3,063,310 - -0.4 3,063,310 - -0.7 3,063,310 - +0.1 3,063,475 + ABZR87_RS14505 0.18 -1.5 3,063,476 - ABZR87_RS14505 0.18 -1.8 3,063,635 - ABZR87_RS14505 0.40 -1.4 3,063,635 - ABZR87_RS14505 0.40 -0.4 3,063,931 + ABZR87_RS14505 0.83 -0.8 3,063,950 - ABZR87_RS14505 0.85 -0.8 3,063,950 - ABZR87_RS14505 0.85 +0.6 3,063,950 - ABZR87_RS14505 0.85 -1.0 3,064,038 - -0.1 3,064,167 + ABZR87_RS14510 0.12 -1.0 3,064,167 + ABZR87_RS14510 0.12 -1.3 3,064,167 + ABZR87_RS14510 0.12 +0.9 3,064,168 - ABZR87_RS14510 0.12 +1.3 3,064,369 - ABZR87_RS14510 0.37 +1.2 3,064,369 - ABZR87_RS14510 0.37 -1.8 3,064,369 - ABZR87_RS14510 0.37 -0.9 3,064,369 - ABZR87_RS14510 0.37 -0.1 3,064,557 + ABZR87_RS14510 0.59 -0.5 3,064,557 + ABZR87_RS14510 0.59 -1.4 3,064,558 - ABZR87_RS14510 0.60 +2.1 3,064,558 - ABZR87_RS14510 0.60 -0.3 3,064,560 + ABZR87_RS14510 0.60 +1.1 3,064,560 + ABZR87_RS14510 0.60 -1.1 3,064,561 - ABZR87_RS14510 0.60 +1.1 3,064,561 - ABZR87_RS14510 0.60 -2.0 3,064,563 + ABZR87_RS14510 0.60 +0.6 3,064,564 - ABZR87_RS14510 0.60 -1.3
Or see this region's nucleotide sequence