Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS14365 and ABZR87_RS14370 are separated by 89 nucleotides ABZR87_RS14370 and ABZR87_RS14375 are separated by 189 nucleotides ABZR87_RS14375 and ABZR87_RS14380 are separated by 19 nucleotides ABZR87_RS14380 and ABZR87_RS14385 are separated by 41 nucleotides
ABZR87_RS14365: ABZR87_RS14365 - DMT family transporter, at 3,037,660 to 3,038,631
_RS14365
ABZR87_RS14370: ABZR87_RS14370 - TIGR00645 family protein, at 3,038,721 to 3,039,260
_RS14370
ABZR87_RS14375: ABZR87_RS14375 - fumarate hydratase, at 3,039,450 to 3,040,973
_RS14375
ABZR87_RS14380: ABZR87_RS14380 - glutamate racemase, at 3,040,993 to 3,041,841
_RS14380
ABZR87_RS14385: ABZR87_RS14385 - dicarboxylate/amino acid:cation symporter, at 3,041,883 to 3,043,151
_RS14385
Position (kb)
3039
3040
3041 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3038.558 kb on + strand at 3038.681 kb on + strand at 3038.797 kb on - strand, within ABZR87_RS14370 at 3038.797 kb on - strand, within ABZR87_RS14370 at 3038.797 kb on - strand, within ABZR87_RS14370 at 3038.826 kb on + strand, within ABZR87_RS14370 at 3038.826 kb on + strand, within ABZR87_RS14370 at 3038.826 kb on + strand, within ABZR87_RS14370 at 3038.826 kb on + strand, within ABZR87_RS14370 at 3038.826 kb on + strand, within ABZR87_RS14370 at 3038.827 kb on - strand, within ABZR87_RS14370 at 3038.827 kb on - strand, within ABZR87_RS14370 at 3038.832 kb on + strand, within ABZR87_RS14370 at 3038.832 kb on + strand, within ABZR87_RS14370 at 3038.832 kb on + strand, within ABZR87_RS14370 at 3038.832 kb on + strand, within ABZR87_RS14370 at 3038.833 kb on - strand, within ABZR87_RS14370 at 3039.329 kb on + strand at 3039.329 kb on + strand at 3039.329 kb on + strand at 3039.330 kb on - strand at 3039.330 kb on - strand at 3039.330 kb on - strand at 3039.330 kb on - strand at 3039.431 kb on + strand at 3039.431 kb on + strand at 3039.432 kb on - strand at 3039.433 kb on + strand at 3039.433 kb on + strand at 3039.433 kb on + strand at 3039.433 kb on + strand at 3039.433 kb on + strand at 3039.433 kb on + strand at 3039.434 kb on - strand at 3039.434 kb on - strand at 3039.434 kb on - strand at 3039.434 kb on - strand at 3039.434 kb on - strand at 3039.505 kb on - strand at 3039.505 kb on - strand at 3039.505 kb on - strand at 3039.513 kb on + strand at 3039.514 kb on - strand at 3039.532 kb on - strand at 3039.532 kb on - strand at 3039.555 kb on + strand at 3039.756 kb on + strand, within ABZR87_RS14375 at 3040.152 kb on + strand, within ABZR87_RS14375 at 3040.153 kb on - strand, within ABZR87_RS14375 at 3040.153 kb on - strand, within ABZR87_RS14375 at 3040.570 kb on - strand, within ABZR87_RS14375 at 3040.570 kb on - strand, within ABZR87_RS14375 at 3040.572 kb on + strand, within ABZR87_RS14375 at 3040.572 kb on + strand, within ABZR87_RS14375 at 3040.573 kb on - strand, within ABZR87_RS14375 at 3040.779 kb on + strand, within ABZR87_RS14375 at 3040.780 kb on - strand, within ABZR87_RS14375 at 3040.848 kb on + strand at 3040.848 kb on + strand at 3040.992 kb on + strand at 3040.992 kb on + strand at 3040.993 kb on - strand at 3040.993 kb on - strand at 3041.098 kb on + strand, within ABZR87_RS14380 at 3041.098 kb on + strand, within ABZR87_RS14380 at 3041.146 kb on + strand, within ABZR87_RS14380 at 3041.146 kb on + strand, within ABZR87_RS14380 at 3041.147 kb on - strand, within ABZR87_RS14380 at 3041.147 kb on - strand, within ABZR87_RS14380 at 3041.147 kb on - strand, within ABZR87_RS14380 at 3041.147 kb on - strand, within ABZR87_RS14380 at 3041.500 kb on + strand, within ABZR87_RS14380 at 3041.501 kb on - strand, within ABZR87_RS14380 at 3041.557 kb on + strand, within ABZR87_RS14380 at 3041.558 kb on - strand, within ABZR87_RS14380 at 3041.558 kb on - strand, within ABZR87_RS14380 at 3041.941 kb on + strand at 3041.941 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 3,038,558 + +0.5 3,038,681 + -0.4 3,038,797 - ABZR87_RS14370 0.14 -3.1 3,038,797 - ABZR87_RS14370 0.14 -0.9 3,038,797 - ABZR87_RS14370 0.14 +0.3 3,038,826 + ABZR87_RS14370 0.19 +0.3 3,038,826 + ABZR87_RS14370 0.19 +0.1 3,038,826 + ABZR87_RS14370 0.19 +0.7 3,038,826 + ABZR87_RS14370 0.19 +0.5 3,038,826 + ABZR87_RS14370 0.19 +0.5 3,038,827 - ABZR87_RS14370 0.20 -0.6 3,038,827 - ABZR87_RS14370 0.20 -3.2 3,038,832 + ABZR87_RS14370 0.21 -0.2 3,038,832 + ABZR87_RS14370 0.21 -0.2 3,038,832 + ABZR87_RS14370 0.21 -0.4 3,038,832 + ABZR87_RS14370 0.21 -0.5 3,038,833 - ABZR87_RS14370 0.21 -0.3 3,039,329 + -0.4 3,039,329 + +0.8 3,039,329 + -0.4 3,039,330 - +0.8 3,039,330 - +0.1 3,039,330 - -0.9 3,039,330 - +1.4 3,039,431 + +0.6 3,039,431 + +0.2 3,039,432 - +0.7 3,039,433 + +1.4 3,039,433 + +0.2 3,039,433 + +0.3 3,039,433 + +0.6 3,039,433 + -1.5 3,039,433 + +0.6 3,039,434 - -0.2 3,039,434 - -1.8 3,039,434 - -0.4 3,039,434 - +0.2 3,039,434 - +0.9 3,039,505 - +0.4 3,039,505 - +0.3 3,039,505 - -1.5 3,039,513 + +0.5 3,039,514 - +0.6 3,039,532 - -1.9 3,039,532 - -1.4 3,039,555 + +0.5 3,039,756 + ABZR87_RS14375 0.20 -0.3 3,040,152 + ABZR87_RS14375 0.46 -0.5 3,040,153 - ABZR87_RS14375 0.46 -1.6 3,040,153 - ABZR87_RS14375 0.46 -1.7 3,040,570 - ABZR87_RS14375 0.73 -1.4 3,040,570 - ABZR87_RS14375 0.73 -0.8 3,040,572 + ABZR87_RS14375 0.74 -0.7 3,040,572 + ABZR87_RS14375 0.74 -0.0 3,040,573 - ABZR87_RS14375 0.74 -1.3 3,040,779 + ABZR87_RS14375 0.87 +1.2 3,040,780 - ABZR87_RS14375 0.87 +0.5 3,040,848 + -0.9 3,040,848 + -0.5 3,040,992 + +0.8 3,040,992 + -1.0 3,040,993 - +0.4 3,040,993 - -2.9 3,041,098 + ABZR87_RS14380 0.12 +1.1 3,041,098 + ABZR87_RS14380 0.12 -1.3 3,041,146 + ABZR87_RS14380 0.18 -0.2 3,041,146 + ABZR87_RS14380 0.18 +0.1 3,041,147 - ABZR87_RS14380 0.18 +0.2 3,041,147 - ABZR87_RS14380 0.18 -0.0 3,041,147 - ABZR87_RS14380 0.18 +0.3 3,041,147 - ABZR87_RS14380 0.18 -0.8 3,041,500 + ABZR87_RS14380 0.60 +1.0 3,041,501 - ABZR87_RS14380 0.60 +0.8 3,041,557 + ABZR87_RS14380 0.66 -0.9 3,041,558 - ABZR87_RS14380 0.67 +0.5 3,041,558 - ABZR87_RS14380 0.67 -1.1 3,041,941 + +0.2 3,041,941 + -1.4
Or see this region's nucleotide sequence