Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS12790 and ABZR87_RS12795 are separated by 192 nucleotides ABZR87_RS12795 and ABZR87_RS12800 are separated by 41 nucleotides ABZR87_RS12800 and ABZR87_RS12805 are separated by 244 nucleotides
ABZR87_RS12790: ABZR87_RS12790 - ATP-dependent Clp protease ATP-binding subunit ClpX, at 2,717,916 to 2,719,190
_RS12790
ABZR87_RS12795: ABZR87_RS12795 - ATP-dependent Clp endopeptidase proteolytic subunit ClpP, at 2,719,383 to 2,720,030
_RS12795
ABZR87_RS12800: ABZR87_RS12800 - trigger factor, at 2,720,072 to 2,721,415
_RS12800
ABZR87_RS12805: ABZR87_RS12805 - bifunctional tRNA (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase MnmD/FAD-dependent 5-carboxymethylaminomethyl-2-thiouridine(34) oxidoreductase MnmC, at 2,721,660 to 2,723,678
_RS12805
Position (kb)
2720
2721
2722 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2719.151 kb on - strand at 2719.197 kb on + strand at 2719.198 kb on - strand at 2719.204 kb on - strand at 2719.221 kb on + strand at 2719.222 kb on - strand at 2719.222 kb on - strand at 2719.222 kb on - strand at 2719.222 kb on - strand at 2719.222 kb on - strand at 2719.232 kb on - strand at 2719.260 kb on + strand at 2719.260 kb on + strand at 2719.333 kb on + strand at 2719.354 kb on + strand at 2719.354 kb on + strand at 2719.354 kb on + strand at 2719.354 kb on + strand at 2719.354 kb on + strand at 2719.354 kb on + strand at 2719.354 kb on + strand at 2719.354 kb on + strand at 2719.354 kb on + strand at 2719.355 kb on - strand at 2719.355 kb on - strand at 2719.355 kb on - strand at 2719.355 kb on - strand at 2719.355 kb on - strand at 2719.371 kb on + strand at 2719.371 kb on + strand at 2719.371 kb on + strand at 2719.372 kb on - strand at 2719.476 kb on - strand, within ABZR87_RS12795 at 2719.531 kb on + strand, within ABZR87_RS12795 at 2719.532 kb on - strand, within ABZR87_RS12795 at 2719.532 kb on - strand, within ABZR87_RS12795 at 2719.732 kb on + strand, within ABZR87_RS12795 at 2719.732 kb on + strand, within ABZR87_RS12795 at 2719.732 kb on + strand, within ABZR87_RS12795 at 2719.733 kb on - strand, within ABZR87_RS12795 at 2719.777 kb on + strand, within ABZR87_RS12795 at 2719.778 kb on - strand, within ABZR87_RS12795 at 2719.778 kb on - strand, within ABZR87_RS12795 at 2719.903 kb on + strand, within ABZR87_RS12795 at 2719.903 kb on + strand, within ABZR87_RS12795 at 2719.904 kb on - strand, within ABZR87_RS12795 at 2719.904 kb on - strand, within ABZR87_RS12795 at 2719.907 kb on + strand, within ABZR87_RS12795 at 2719.907 kb on + strand, within ABZR87_RS12795 at 2719.907 kb on + strand, within ABZR87_RS12795 at 2719.907 kb on + strand, within ABZR87_RS12795 at 2719.908 kb on - strand, within ABZR87_RS12795 at 2719.908 kb on - strand, within ABZR87_RS12795 at 2719.908 kb on - strand, within ABZR87_RS12795 at 2719.908 kb on - strand, within ABZR87_RS12795 at 2720.047 kb on + strand at 2720.048 kb on - strand at 2720.048 kb on - strand at 2720.048 kb on - strand at 2720.048 kb on - strand at 2720.048 kb on - strand at 2720.048 kb on - strand at 2720.048 kb on - strand at 2720.175 kb on + strand at 2720.175 kb on + strand at 2720.212 kb on - strand, within ABZR87_RS12800 at 2720.212 kb on - strand, within ABZR87_RS12800 at 2720.214 kb on + strand, within ABZR87_RS12800 at 2720.214 kb on + strand, within ABZR87_RS12800 at 2720.215 kb on - strand, within ABZR87_RS12800 at 2720.244 kb on + strand, within ABZR87_RS12800 at 2720.245 kb on - strand, within ABZR87_RS12800 at 2720.724 kb on + strand, within ABZR87_RS12800 at 2720.724 kb on + strand, within ABZR87_RS12800 at 2720.724 kb on + strand, within ABZR87_RS12800 at 2720.724 kb on + strand, within ABZR87_RS12800 at 2720.725 kb on - strand, within ABZR87_RS12800 at 2720.807 kb on - strand, within ABZR87_RS12800 at 2720.964 kb on + strand, within ABZR87_RS12800 at 2720.964 kb on + strand, within ABZR87_RS12800 at 2720.965 kb on - strand, within ABZR87_RS12800 at 2720.965 kb on - strand, within ABZR87_RS12800 at 2721.082 kb on - strand, within ABZR87_RS12800 at 2721.247 kb on - strand, within ABZR87_RS12800 at 2721.422 kb on - strand at 2721.422 kb on - strand at 2721.422 kb on - strand at 2721.588 kb on - strand at 2721.588 kb on - strand at 2721.590 kb on + strand at 2721.661 kb on + strand at 2721.661 kb on + strand at 2721.662 kb on - strand at 2721.662 kb on - strand at 2721.662 kb on - strand at 2721.662 kb on - strand at 2721.862 kb on + strand, within ABZR87_RS12805 at 2721.863 kb on - strand, within ABZR87_RS12805 at 2721.877 kb on + strand, within ABZR87_RS12805 at 2721.877 kb on + strand, within ABZR87_RS12805 at 2721.878 kb on - strand, within ABZR87_RS12805 at 2721.878 kb on - strand, within ABZR87_RS12805 at 2721.878 kb on - strand, within ABZR87_RS12805 at 2721.878 kb on - strand, within ABZR87_RS12805 at 2721.878 kb on - strand, within ABZR87_RS12805 at 2721.878 kb on - strand, within ABZR87_RS12805 at 2722.009 kb on + strand, within ABZR87_RS12805 at 2722.010 kb on - strand, within ABZR87_RS12805 at 2722.010 kb on - strand, within ABZR87_RS12805 at 2722.010 kb on - strand, within ABZR87_RS12805 at 2722.149 kb on + strand, within ABZR87_RS12805 at 2722.149 kb on + strand, within ABZR87_RS12805 at 2722.189 kb on + strand, within ABZR87_RS12805 at 2722.190 kb on - strand, within ABZR87_RS12805 at 2722.325 kb on + strand, within ABZR87_RS12805 at 2722.325 kb on + strand, within ABZR87_RS12805 at 2722.326 kb on - strand, within ABZR87_RS12805 at 2722.326 kb on - strand, within ABZR87_RS12805 at 2722.326 kb on - strand, within ABZR87_RS12805
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 2,719,151 - -1.3 2,719,197 + -1.9 2,719,198 - -0.8 2,719,204 - -0.6 2,719,221 + -0.3 2,719,222 - -0.2 2,719,222 - +0.2 2,719,222 - +1.7 2,719,222 - +0.4 2,719,222 - -0.1 2,719,232 - +0.0 2,719,260 + +0.2 2,719,260 + -0.6 2,719,333 + +0.6 2,719,354 + -0.2 2,719,354 + -1.5 2,719,354 + +0.7 2,719,354 + -1.1 2,719,354 + -0.6 2,719,354 + +0.4 2,719,354 + +0.0 2,719,354 + -0.1 2,719,354 + +0.4 2,719,355 - +0.6 2,719,355 - -0.6 2,719,355 - +0.0 2,719,355 - -0.4 2,719,355 - -1.8 2,719,371 + -0.1 2,719,371 + +0.7 2,719,371 + +1.2 2,719,372 - -0.1 2,719,476 - ABZR87_RS12795 0.14 +0.5 2,719,531 + ABZR87_RS12795 0.23 +0.7 2,719,532 - ABZR87_RS12795 0.23 -1.4 2,719,532 - ABZR87_RS12795 0.23 +2.6 2,719,732 + ABZR87_RS12795 0.54 +0.0 2,719,732 + ABZR87_RS12795 0.54 -2.1 2,719,732 + ABZR87_RS12795 0.54 +0.2 2,719,733 - ABZR87_RS12795 0.54 -1.3 2,719,777 + ABZR87_RS12795 0.61 -0.4 2,719,778 - ABZR87_RS12795 0.61 -1.0 2,719,778 - ABZR87_RS12795 0.61 +0.4 2,719,903 + ABZR87_RS12795 0.80 +0.3 2,719,903 + ABZR87_RS12795 0.80 -0.6 2,719,904 - ABZR87_RS12795 0.80 -0.7 2,719,904 - ABZR87_RS12795 0.80 -0.6 2,719,907 + ABZR87_RS12795 0.81 -0.6 2,719,907 + ABZR87_RS12795 0.81 -0.5 2,719,907 + ABZR87_RS12795 0.81 +0.0 2,719,907 + ABZR87_RS12795 0.81 +0.4 2,719,908 - ABZR87_RS12795 0.81 -2.5 2,719,908 - ABZR87_RS12795 0.81 -0.6 2,719,908 - ABZR87_RS12795 0.81 +0.4 2,719,908 - ABZR87_RS12795 0.81 -1.4 2,720,047 + -1.1 2,720,048 - -0.5 2,720,048 - +0.1 2,720,048 - -0.4 2,720,048 - -0.5 2,720,048 - -1.4 2,720,048 - +0.8 2,720,048 - +0.4 2,720,175 + -2.1 2,720,175 + -1.2 2,720,212 - ABZR87_RS12800 0.10 -1.6 2,720,212 - ABZR87_RS12800 0.10 -0.2 2,720,214 + ABZR87_RS12800 0.11 +0.4 2,720,214 + ABZR87_RS12800 0.11 -1.2 2,720,215 - ABZR87_RS12800 0.11 +0.8 2,720,244 + ABZR87_RS12800 0.13 -2.3 2,720,245 - ABZR87_RS12800 0.13 +0.6 2,720,724 + ABZR87_RS12800 0.49 -0.1 2,720,724 + ABZR87_RS12800 0.49 -0.6 2,720,724 + ABZR87_RS12800 0.49 -1.5 2,720,724 + ABZR87_RS12800 0.49 -2.0 2,720,725 - ABZR87_RS12800 0.49 -2.1 2,720,807 - ABZR87_RS12800 0.55 -0.6 2,720,964 + ABZR87_RS12800 0.66 -1.1 2,720,964 + ABZR87_RS12800 0.66 -0.3 2,720,965 - ABZR87_RS12800 0.66 -0.4 2,720,965 - ABZR87_RS12800 0.66 +1.0 2,721,082 - ABZR87_RS12800 0.75 -0.7 2,721,247 - ABZR87_RS12800 0.87 +0.7 2,721,422 - +0.0 2,721,422 - -2.0 2,721,422 - -3.8 2,721,588 - -0.1 2,721,588 - -0.9 2,721,590 + -0.0 2,721,661 + -0.3 2,721,661 + -0.8 2,721,662 - -1.5 2,721,662 - +0.0 2,721,662 - -0.0 2,721,662 - -0.2 2,721,862 + ABZR87_RS12805 0.10 -0.6 2,721,863 - ABZR87_RS12805 0.10 -0.9 2,721,877 + ABZR87_RS12805 0.11 +0.5 2,721,877 + ABZR87_RS12805 0.11 +0.9 2,721,878 - ABZR87_RS12805 0.11 -0.8 2,721,878 - ABZR87_RS12805 0.11 -0.3 2,721,878 - ABZR87_RS12805 0.11 -1.4 2,721,878 - ABZR87_RS12805 0.11 +1.4 2,721,878 - ABZR87_RS12805 0.11 +0.4 2,721,878 - ABZR87_RS12805 0.11 +0.1 2,722,009 + ABZR87_RS12805 0.17 +0.3 2,722,010 - ABZR87_RS12805 0.17 +0.1 2,722,010 - ABZR87_RS12805 0.17 -2.8 2,722,010 - ABZR87_RS12805 0.17 +0.4 2,722,149 + ABZR87_RS12805 0.24 -0.4 2,722,149 + ABZR87_RS12805 0.24 +0.2 2,722,189 + ABZR87_RS12805 0.26 -0.7 2,722,190 - ABZR87_RS12805 0.26 -0.1 2,722,325 + ABZR87_RS12805 0.33 -0.5 2,722,325 + ABZR87_RS12805 0.33 +0.5 2,722,326 - ABZR87_RS12805 0.33 +0.4 2,722,326 - ABZR87_RS12805 0.33 -0.4 2,722,326 - ABZR87_RS12805 0.33 +0.2
Or see this region's nucleotide sequence