Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS11740 and ABZR87_RS11745 are separated by 17 nucleotides ABZR87_RS11745 and ABZR87_RS11750 are separated by 10 nucleotides ABZR87_RS11750 and ABZR87_RS11755 are separated by 37 nucleotides ABZR87_RS11755 and ABZR87_RS11760 are separated by 14 nucleotides ABZR87_RS11760 and ABZR87_RS11765 are separated by 60 nucleotides
ABZR87_RS11740: ABZR87_RS11740 - 50S ribosomal protein L3 N(5)-glutamine methyltransferase, at 2,489,230 to 2,490,132
_RS11740
ABZR87_RS11745: ABZR87_RS11745 - class IV adenylate cyclase, at 2,490,150 to 2,490,668
_RS11745
ABZR87_RS11750: ABZR87_RS11750 - succinyl-diaminopimelate desuccinylase, at 2,490,679 to 2,491,830
_RS11750
ABZR87_RS11755: ABZR87_RS11755 - arsenate reductase, at 2,491,868 to 2,492,212
_RS11755
ABZR87_RS11760: ABZR87_RS11760 - membrane protein, at 2,492,227 to 2,492,640
_RS11760
ABZR87_RS11765: ABZR87_RS11765 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase, at 2,492,701 to 2,493,528
_RS11765
Position (kb)
2490
2491
2492 Strain fitness (log2 ratio)
-1
0
1 at 2489.720 kb on - strand, within ABZR87_RS11740 at 2489.822 kb on + strand, within ABZR87_RS11740 at 2489.822 kb on + strand, within ABZR87_RS11740 at 2489.823 kb on - strand, within ABZR87_RS11740 at 2489.861 kb on + strand, within ABZR87_RS11740 at 2489.861 kb on + strand, within ABZR87_RS11740 at 2489.862 kb on - strand, within ABZR87_RS11740 at 2489.862 kb on - strand, within ABZR87_RS11740 at 2489.996 kb on + strand, within ABZR87_RS11740 at 2489.996 kb on + strand, within ABZR87_RS11740 at 2489.996 kb on + strand, within ABZR87_RS11740 at 2489.997 kb on - strand, within ABZR87_RS11740 at 2490.072 kb on - strand at 2490.072 kb on - strand at 2490.181 kb on + strand at 2490.181 kb on + strand at 2490.181 kb on + strand at 2490.246 kb on + strand, within ABZR87_RS11745 at 2490.269 kb on + strand, within ABZR87_RS11745 at 2490.353 kb on - strand, within ABZR87_RS11745 at 2490.448 kb on + strand, within ABZR87_RS11745 at 2490.449 kb on - strand, within ABZR87_RS11745 at 2490.487 kb on + strand, within ABZR87_RS11745 at 2490.487 kb on + strand, within ABZR87_RS11745 at 2490.487 kb on + strand, within ABZR87_RS11745 at 2490.488 kb on - strand, within ABZR87_RS11745 at 2490.488 kb on - strand, within ABZR87_RS11745 at 2491.870 kb on - strand at 2491.885 kb on - strand at 2492.133 kb on + strand, within ABZR87_RS11755 at 2492.134 kb on - strand, within ABZR87_RS11755 at 2492.436 kb on + strand, within ABZR87_RS11760 at 2492.676 kb on + strand at 2492.676 kb on + strand at 2492.703 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 2,489,720 - ABZR87_RS11740 0.54 -0.4 2,489,822 + ABZR87_RS11740 0.66 -1.5 2,489,822 + ABZR87_RS11740 0.66 -0.5 2,489,823 - ABZR87_RS11740 0.66 -0.4 2,489,861 + ABZR87_RS11740 0.70 -0.6 2,489,861 + ABZR87_RS11740 0.70 -1.1 2,489,862 - ABZR87_RS11740 0.70 -1.6 2,489,862 - ABZR87_RS11740 0.70 -0.6 2,489,996 + ABZR87_RS11740 0.85 +0.1 2,489,996 + ABZR87_RS11740 0.85 +1.0 2,489,996 + ABZR87_RS11740 0.85 +0.4 2,489,997 - ABZR87_RS11740 0.85 -0.3 2,490,072 - -0.5 2,490,072 - +0.7 2,490,181 + -1.0 2,490,181 + -0.2 2,490,181 + -1.0 2,490,246 + ABZR87_RS11745 0.18 -0.1 2,490,269 + ABZR87_RS11745 0.23 -0.1 2,490,353 - ABZR87_RS11745 0.39 +0.5 2,490,448 + ABZR87_RS11745 0.57 -0.3 2,490,449 - ABZR87_RS11745 0.58 -0.1 2,490,487 + ABZR87_RS11745 0.65 +0.5 2,490,487 + ABZR87_RS11745 0.65 -0.5 2,490,487 + ABZR87_RS11745 0.65 -0.6 2,490,488 - ABZR87_RS11745 0.65 -1.0 2,490,488 - ABZR87_RS11745 0.65 -0.2 2,491,870 - +0.2 2,491,885 - +0.0 2,492,133 + ABZR87_RS11755 0.77 +0.2 2,492,134 - ABZR87_RS11755 0.77 -0.8 2,492,436 + ABZR87_RS11760 0.50 +1.0 2,492,676 + +0.5 2,492,676 + -1.1 2,492,703 - +0.2
Or see this region's nucleotide sequence