Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS11605 and ABZR87_RS11610 are separated by 172 nucleotides ABZR87_RS11610 and ABZR87_RS11615 are separated by 84 nucleotides
ABZR87_RS11605: ABZR87_RS11605 - NCS1 family nucleobase:cation symporter-1, at 2,459,474 to 2,460,964
_RS11605
ABZR87_RS11610: ABZR87_RS11610 - universal stress protein, at 2,461,137 to 2,461,571
_RS11610
ABZR87_RS11615: ABZR87_RS11615 - LysR family transcriptional regulator, at 2,461,656 to 2,462,585
_RS11615
Position (kb)
2461
2462 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2460.195 kb on + strand, within ABZR87_RS11605 at 2460.196 kb on - strand, within ABZR87_RS11605 at 2460.196 kb on - strand, within ABZR87_RS11605 at 2460.252 kb on + strand, within ABZR87_RS11605 at 2460.252 kb on + strand, within ABZR87_RS11605 at 2460.253 kb on - strand, within ABZR87_RS11605 at 2460.336 kb on + strand, within ABZR87_RS11605 at 2460.336 kb on + strand, within ABZR87_RS11605 at 2460.336 kb on + strand, within ABZR87_RS11605 at 2460.336 kb on + strand, within ABZR87_RS11605 at 2460.337 kb on - strand, within ABZR87_RS11605 at 2460.543 kb on + strand, within ABZR87_RS11605 at 2460.543 kb on + strand, within ABZR87_RS11605 at 2460.543 kb on + strand, within ABZR87_RS11605 at 2460.543 kb on + strand, within ABZR87_RS11605 at 2460.544 kb on - strand, within ABZR87_RS11605 at 2460.544 kb on - strand, within ABZR87_RS11605 at 2460.544 kb on - strand, within ABZR87_RS11605 at 2460.719 kb on + strand, within ABZR87_RS11605 at 2460.825 kb on + strand at 2460.825 kb on + strand at 2460.826 kb on - strand at 2460.826 kb on - strand at 2461.111 kb on + strand at 2461.111 kb on + strand at 2461.111 kb on + strand at 2461.112 kb on - strand at 2461.115 kb on + strand at 2461.115 kb on + strand at 2461.166 kb on + strand at 2461.288 kb on + strand, within ABZR87_RS11610 at 2461.288 kb on + strand, within ABZR87_RS11610 at 2461.288 kb on + strand, within ABZR87_RS11610 at 2461.288 kb on + strand, within ABZR87_RS11610 at 2461.288 kb on + strand, within ABZR87_RS11610 at 2461.288 kb on + strand, within ABZR87_RS11610 at 2461.288 kb on + strand, within ABZR87_RS11610 at 2461.289 kb on - strand, within ABZR87_RS11610 at 2461.289 kb on - strand, within ABZR87_RS11610 at 2461.289 kb on - strand, within ABZR87_RS11610 at 2461.289 kb on - strand, within ABZR87_RS11610 at 2461.441 kb on + strand, within ABZR87_RS11610 at 2461.442 kb on - strand, within ABZR87_RS11610 at 2461.448 kb on - strand, within ABZR87_RS11610 at 2461.465 kb on + strand, within ABZR87_RS11610 at 2461.465 kb on + strand, within ABZR87_RS11610 at 2461.465 kb on + strand, within ABZR87_RS11610 at 2461.466 kb on - strand, within ABZR87_RS11610 at 2461.619 kb on - strand at 2461.657 kb on + strand at 2461.657 kb on + strand at 2461.658 kb on - strand at 2461.658 kb on - strand at 2461.658 kb on - strand at 2461.658 kb on - strand at 2461.693 kb on + strand at 2461.694 kb on - strand at 2461.708 kb on + strand at 2461.708 kb on + strand at 2461.708 kb on + strand at 2461.708 kb on + strand at 2461.708 kb on + strand at 2461.709 kb on - strand at 2461.709 kb on - strand at 2461.709 kb on - strand at 2461.709 kb on - strand at 2461.709 kb on - strand at 2462.074 kb on + strand, within ABZR87_RS11615 at 2462.074 kb on + strand, within ABZR87_RS11615 at 2462.075 kb on - strand, within ABZR87_RS11615 at 2462.075 kb on - strand, within ABZR87_RS11615 at 2462.530 kb on - strand at 2462.530 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 2,460,195 + ABZR87_RS11605 0.48 -0.5 2,460,196 - ABZR87_RS11605 0.48 +0.3 2,460,196 - ABZR87_RS11605 0.48 +0.4 2,460,252 + ABZR87_RS11605 0.52 -0.7 2,460,252 + ABZR87_RS11605 0.52 +0.3 2,460,253 - ABZR87_RS11605 0.52 -1.1 2,460,336 + ABZR87_RS11605 0.58 +0.4 2,460,336 + ABZR87_RS11605 0.58 +0.4 2,460,336 + ABZR87_RS11605 0.58 +0.1 2,460,336 + ABZR87_RS11605 0.58 -1.5 2,460,337 - ABZR87_RS11605 0.58 +0.2 2,460,543 + ABZR87_RS11605 0.72 -0.0 2,460,543 + ABZR87_RS11605 0.72 +0.1 2,460,543 + ABZR87_RS11605 0.72 -0.6 2,460,543 + ABZR87_RS11605 0.72 -0.3 2,460,544 - ABZR87_RS11605 0.72 -0.9 2,460,544 - ABZR87_RS11605 0.72 +0.3 2,460,544 - ABZR87_RS11605 0.72 +0.9 2,460,719 + ABZR87_RS11605 0.84 +0.4 2,460,825 + +0.3 2,460,825 + +0.8 2,460,826 - -0.6 2,460,826 - -0.7 2,461,111 + -0.5 2,461,111 + +0.4 2,461,111 + +0.9 2,461,112 - -0.1 2,461,115 + -0.3 2,461,115 + +0.2 2,461,166 + +0.1 2,461,288 + ABZR87_RS11610 0.35 +0.1 2,461,288 + ABZR87_RS11610 0.35 +0.0 2,461,288 + ABZR87_RS11610 0.35 -0.4 2,461,288 + ABZR87_RS11610 0.35 -0.1 2,461,288 + ABZR87_RS11610 0.35 +0.1 2,461,288 + ABZR87_RS11610 0.35 +0.2 2,461,288 + ABZR87_RS11610 0.35 +0.4 2,461,289 - ABZR87_RS11610 0.35 +0.5 2,461,289 - ABZR87_RS11610 0.35 +1.7 2,461,289 - ABZR87_RS11610 0.35 -0.3 2,461,289 - ABZR87_RS11610 0.35 -0.2 2,461,441 + ABZR87_RS11610 0.70 -0.3 2,461,442 - ABZR87_RS11610 0.70 -1.9 2,461,448 - ABZR87_RS11610 0.71 +0.5 2,461,465 + ABZR87_RS11610 0.75 -0.9 2,461,465 + ABZR87_RS11610 0.75 -0.8 2,461,465 + ABZR87_RS11610 0.75 -1.1 2,461,466 - ABZR87_RS11610 0.76 -0.3 2,461,619 - +1.4 2,461,657 + +0.2 2,461,657 + +1.0 2,461,658 - +1.1 2,461,658 - -0.9 2,461,658 - -2.8 2,461,658 - +0.8 2,461,693 + +0.4 2,461,694 - +0.1 2,461,708 + -0.7 2,461,708 + -1.6 2,461,708 + +1.1 2,461,708 + +0.4 2,461,708 + +0.0 2,461,709 - -0.7 2,461,709 - -1.0 2,461,709 - -0.5 2,461,709 - -1.0 2,461,709 - -1.5 2,462,074 + ABZR87_RS11615 0.45 -1.7 2,462,074 + ABZR87_RS11615 0.45 -1.2 2,462,075 - ABZR87_RS11615 0.45 -0.3 2,462,075 - ABZR87_RS11615 0.45 -0.7 2,462,530 - -1.1 2,462,530 - -0.1
Or see this region's nucleotide sequence