Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS10920 and ABZR87_RS10925 are separated by 65 nucleotides ABZR87_RS10925 and ABZR87_RS10930 are separated by 48 nucleotides ABZR87_RS10930 and ABZR87_RS10935 are separated by 195 nucleotides
ABZR87_RS10920: ABZR87_RS10920 - histidine--tRNA ligase, at 2,312,529 to 2,313,851
_RS10920
ABZR87_RS10925: ABZR87_RS10925 - tetratricopeptide repeat protein, at 2,313,917 to 2,314,552
_RS10925
ABZR87_RS10930: ABZR87_RS10930 - outer membrane protein assembly factor BamB, at 2,314,601 to 2,315,779
_RS10930
ABZR87_RS10935: ABZR87_RS10935 - ribosome biogenesis GTPase Der, at 2,315,975 to 2,317,318
_RS10935
Position (kb)
2314
2315
2316 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2313.923 kb on + strand at 2313.924 kb on - strand at 2313.948 kb on + strand at 2313.948 kb on + strand at 2313.948 kb on + strand at 2313.949 kb on - strand at 2314.085 kb on + strand, within ABZR87_RS10925 at 2314.095 kb on + strand, within ABZR87_RS10925 at 2314.095 kb on + strand, within ABZR87_RS10925 at 2314.178 kb on + strand, within ABZR87_RS10925 at 2314.217 kb on + strand, within ABZR87_RS10925 at 2314.289 kb on + strand, within ABZR87_RS10925 at 2314.289 kb on + strand, within ABZR87_RS10925 at 2314.289 kb on + strand, within ABZR87_RS10925 at 2314.344 kb on - strand, within ABZR87_RS10925 at 2314.362 kb on + strand, within ABZR87_RS10925 at 2314.400 kb on + strand, within ABZR87_RS10925 at 2314.463 kb on + strand, within ABZR87_RS10925 at 2314.580 kb on + strand at 2314.847 kb on + strand, within ABZR87_RS10930 at 2314.847 kb on + strand, within ABZR87_RS10930 at 2315.150 kb on + strand, within ABZR87_RS10930 at 2315.521 kb on + strand, within ABZR87_RS10930 at 2315.521 kb on + strand, within ABZR87_RS10930 at 2315.521 kb on + strand, within ABZR87_RS10930 at 2315.759 kb on + strand at 2315.777 kb on + strand at 2315.777 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 2,313,923 + -1.0 2,313,924 - +0.0 2,313,948 + +0.6 2,313,948 + -0.1 2,313,948 + -2.7 2,313,949 - +2.0 2,314,085 + ABZR87_RS10925 0.26 +0.4 2,314,095 + ABZR87_RS10925 0.28 +0.1 2,314,095 + ABZR87_RS10925 0.28 +0.1 2,314,178 + ABZR87_RS10925 0.41 +0.5 2,314,217 + ABZR87_RS10925 0.47 +0.8 2,314,289 + ABZR87_RS10925 0.58 -0.8 2,314,289 + ABZR87_RS10925 0.58 -0.6 2,314,289 + ABZR87_RS10925 0.58 +0.2 2,314,344 - ABZR87_RS10925 0.67 +0.0 2,314,362 + ABZR87_RS10925 0.70 +1.2 2,314,400 + ABZR87_RS10925 0.76 +0.4 2,314,463 + ABZR87_RS10925 0.86 -1.3 2,314,580 + -0.2 2,314,847 + ABZR87_RS10930 0.21 +2.0 2,314,847 + ABZR87_RS10930 0.21 +0.0 2,315,150 + ABZR87_RS10930 0.47 +1.5 2,315,521 + ABZR87_RS10930 0.78 +0.1 2,315,521 + ABZR87_RS10930 0.78 -1.7 2,315,521 + ABZR87_RS10930 0.78 -1.0 2,315,759 + +1.0 2,315,777 + +0.6 2,315,777 + -1.5
Or see this region's nucleotide sequence