Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS10865 and ABZR87_RS10870 are separated by 1 nucleotides ABZR87_RS10870 and ABZR87_RS10875 are separated by 8 nucleotides ABZR87_RS10875 and ABZR87_RS10880 are separated by 37 nucleotides
ABZR87_RS10865: ABZR87_RS10865 - protein-L-isoaspartate(D-aspartate) O-methyltransferase, at 2,301,099 to 2,302,043
_RS10865
ABZR87_RS10870: ABZR87_RS10870 - peptidoglycan DD-metalloendopeptidase family protein, at 2,302,045 to 2,302,872
_RS10870
ABZR87_RS10875: ABZR87_RS10875 - RNA polymerase sigma factor RpoS, at 2,302,881 to 2,304,017
_RS10875
ABZR87_RS10880: ABZR87_RS10880 - endonuclease/exonuclease/phosphatase family protein, at 2,304,055 to 2,304,918
_RS10880
Position (kb)
2302
2303
2304
2305 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2301.992 kb on + strand at 2301.993 kb on - strand at 2302.041 kb on + strand at 2302.041 kb on + strand at 2302.042 kb on - strand at 2302.042 kb on - strand at 2302.042 kb on - strand at 2302.042 kb on - strand at 2302.128 kb on - strand, within ABZR87_RS10870 at 2302.231 kb on + strand, within ABZR87_RS10870 at 2302.231 kb on + strand, within ABZR87_RS10870 at 2302.231 kb on + strand, within ABZR87_RS10870 at 2302.231 kb on + strand, within ABZR87_RS10870 at 2302.231 kb on + strand, within ABZR87_RS10870 at 2302.254 kb on + strand, within ABZR87_RS10870 at 2302.255 kb on - strand, within ABZR87_RS10870 at 2302.261 kb on + strand, within ABZR87_RS10870 at 2302.261 kb on + strand, within ABZR87_RS10870 at 2302.262 kb on - strand, within ABZR87_RS10870 at 2302.262 kb on - strand, within ABZR87_RS10870 at 2302.355 kb on + strand, within ABZR87_RS10870 at 2302.355 kb on + strand, within ABZR87_RS10870 at 2302.422 kb on + strand, within ABZR87_RS10870 at 2302.423 kb on - strand, within ABZR87_RS10870 at 2302.431 kb on + strand, within ABZR87_RS10870 at 2302.432 kb on - strand, within ABZR87_RS10870 at 2302.432 kb on - strand, within ABZR87_RS10870 at 2302.432 kb on - strand, within ABZR87_RS10870 at 2302.709 kb on - strand, within ABZR87_RS10870 at 2302.785 kb on + strand, within ABZR87_RS10870 at 2302.833 kb on + strand at 2302.855 kb on + strand at 2302.870 kb on + strand at 2302.870 kb on + strand at 2302.871 kb on - strand at 2302.942 kb on + strand at 2302.942 kb on + strand at 2302.942 kb on + strand at 2302.942 kb on + strand at 2302.943 kb on - strand at 2302.943 kb on - strand at 2302.943 kb on - strand at 2303.010 kb on - strand, within ABZR87_RS10875 at 2303.010 kb on - strand, within ABZR87_RS10875 at 2303.010 kb on - strand, within ABZR87_RS10875 at 2303.010 kb on - strand, within ABZR87_RS10875 at 2303.047 kb on + strand, within ABZR87_RS10875 at 2303.047 kb on + strand, within ABZR87_RS10875 at 2303.048 kb on - strand, within ABZR87_RS10875 at 2303.048 kb on - strand, within ABZR87_RS10875 at 2303.205 kb on + strand, within ABZR87_RS10875 at 2303.206 kb on - strand, within ABZR87_RS10875 at 2303.206 kb on - strand, within ABZR87_RS10875 at 2303.457 kb on + strand, within ABZR87_RS10875 at 2303.457 kb on + strand, within ABZR87_RS10875 at 2303.457 kb on + strand, within ABZR87_RS10875 at 2303.457 kb on + strand, within ABZR87_RS10875 at 2303.457 kb on + strand, within ABZR87_RS10875 at 2303.457 kb on + strand, within ABZR87_RS10875 at 2303.457 kb on + strand, within ABZR87_RS10875 at 2303.457 kb on + strand, within ABZR87_RS10875 at 2303.458 kb on - strand, within ABZR87_RS10875 at 2303.458 kb on - strand, within ABZR87_RS10875 at 2303.458 kb on - strand, within ABZR87_RS10875 at 2303.458 kb on - strand, within ABZR87_RS10875 at 2303.458 kb on - strand, within ABZR87_RS10875 at 2303.458 kb on - strand, within ABZR87_RS10875 at 2303.458 kb on - strand, within ABZR87_RS10875 at 2303.458 kb on - strand, within ABZR87_RS10875 at 2303.458 kb on - strand, within ABZR87_RS10875 at 2303.458 kb on - strand, within ABZR87_RS10875 at 2303.514 kb on - strand, within ABZR87_RS10875 at 2303.514 kb on - strand, within ABZR87_RS10875 at 2303.514 kb on - strand, within ABZR87_RS10875 at 2303.699 kb on + strand, within ABZR87_RS10875 at 2303.699 kb on + strand, within ABZR87_RS10875 at 2303.844 kb on + strand, within ABZR87_RS10875 at 2303.844 kb on + strand, within ABZR87_RS10875 at 2303.845 kb on - strand, within ABZR87_RS10875 at 2303.845 kb on - strand, within ABZR87_RS10875 at 2303.845 kb on - strand, within ABZR87_RS10875 at 2303.845 kb on - strand, within ABZR87_RS10875 at 2303.848 kb on + strand, within ABZR87_RS10875 at 2303.849 kb on - strand, within ABZR87_RS10875 at 2303.849 kb on - strand, within ABZR87_RS10875 at 2303.874 kb on + strand, within ABZR87_RS10875 at 2303.874 kb on + strand, within ABZR87_RS10875 at 2303.874 kb on + strand, within ABZR87_RS10875 at 2303.874 kb on + strand, within ABZR87_RS10875 at 2303.875 kb on - strand, within ABZR87_RS10875 at 2303.875 kb on - strand, within ABZR87_RS10875 at 2303.875 kb on - strand, within ABZR87_RS10875 at 2303.875 kb on - strand, within ABZR87_RS10875 at 2303.875 kb on - strand, within ABZR87_RS10875 at 2303.875 kb on - strand, within ABZR87_RS10875 at 2303.875 kb on - strand, within ABZR87_RS10875 at 2303.875 kb on - strand, within ABZR87_RS10875 at 2304.000 kb on - strand at 2304.016 kb on - strand at 2304.176 kb on + strand, within ABZR87_RS10880 at 2304.265 kb on + strand, within ABZR87_RS10880 at 2304.265 kb on + strand, within ABZR87_RS10880 at 2304.265 kb on + strand, within ABZR87_RS10880 at 2304.265 kb on + strand, within ABZR87_RS10880 at 2304.266 kb on - strand, within ABZR87_RS10880 at 2304.266 kb on - strand, within ABZR87_RS10880 at 2304.266 kb on - strand, within ABZR87_RS10880 at 2304.491 kb on + strand, within ABZR87_RS10880 at 2304.492 kb on - strand, within ABZR87_RS10880 at 2304.494 kb on + strand, within ABZR87_RS10880 at 2304.495 kb on - strand, within ABZR87_RS10880 at 2304.495 kb on - strand, within ABZR87_RS10880 at 2304.570 kb on + strand, within ABZR87_RS10880 at 2304.570 kb on + strand, within ABZR87_RS10880 at 2304.570 kb on + strand, within ABZR87_RS10880 at 2304.570 kb on + strand, within ABZR87_RS10880 at 2304.571 kb on - strand, within ABZR87_RS10880 at 2304.571 kb on - strand, within ABZR87_RS10880 at 2304.571 kb on - strand, within ABZR87_RS10880 at 2304.572 kb on + strand, within ABZR87_RS10880 at 2304.572 kb on + strand, within ABZR87_RS10880 at 2304.572 kb on + strand, within ABZR87_RS10880 at 2304.572 kb on + strand, within ABZR87_RS10880 at 2304.572 kb on + strand, within ABZR87_RS10880 at 2304.572 kb on + strand, within ABZR87_RS10880 at 2304.572 kb on + strand, within ABZR87_RS10880 at 2304.572 kb on + strand, within ABZR87_RS10880 at 2304.572 kb on + strand, within ABZR87_RS10880 at 2304.572 kb on + strand, within ABZR87_RS10880 at 2304.572 kb on + strand, within ABZR87_RS10880 at 2304.572 kb on + strand, within ABZR87_RS10880 at 2304.572 kb on + strand, within ABZR87_RS10880 at 2304.572 kb on + strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.598 kb on + strand, within ABZR87_RS10880 at 2304.599 kb on - strand, within ABZR87_RS10880 at 2304.599 kb on - strand, within ABZR87_RS10880 at 2304.599 kb on - strand, within ABZR87_RS10880 at 2304.599 kb on - strand, within ABZR87_RS10880 at 2304.710 kb on + strand, within ABZR87_RS10880 at 2304.710 kb on + strand, within ABZR87_RS10880 at 2304.710 kb on + strand, within ABZR87_RS10880 at 2304.711 kb on - strand, within ABZR87_RS10880 at 2304.711 kb on - strand, within ABZR87_RS10880 at 2304.937 kb on + strand at 2304.937 kb on + strand at 2304.937 kb on + strand at 2304.937 kb on + strand at 2304.937 kb on + strand at 2304.937 kb on + strand at 2304.937 kb on + strand at 2304.937 kb on + strand at 2304.937 kb on + strand at 2304.938 kb on - strand at 2304.938 kb on - strand at 2304.938 kb on - strand at 2304.938 kb on - strand at 2304.938 kb on - strand at 2304.938 kb on - strand at 2304.994 kb on + strand at 2304.995 kb on - strand at 2304.995 kb on - strand at 2304.995 kb on - strand at 2304.995 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 2,301,992 + -0.9 2,301,993 - -1.0 2,302,041 + -0.1 2,302,041 + +0.0 2,302,042 - -1.5 2,302,042 - +0.3 2,302,042 - -0.9 2,302,042 - -1.4 2,302,128 - ABZR87_RS10870 0.10 -0.1 2,302,231 + ABZR87_RS10870 0.22 -1.1 2,302,231 + ABZR87_RS10870 0.22 +1.3 2,302,231 + ABZR87_RS10870 0.22 -0.4 2,302,231 + ABZR87_RS10870 0.22 +0.1 2,302,231 + ABZR87_RS10870 0.22 -2.1 2,302,254 + ABZR87_RS10870 0.25 -0.3 2,302,255 - ABZR87_RS10870 0.25 +0.9 2,302,261 + ABZR87_RS10870 0.26 +0.3 2,302,261 + ABZR87_RS10870 0.26 +0.4 2,302,262 - ABZR87_RS10870 0.26 +0.7 2,302,262 - ABZR87_RS10870 0.26 +0.7 2,302,355 + ABZR87_RS10870 0.37 -0.1 2,302,355 + ABZR87_RS10870 0.37 -1.0 2,302,422 + ABZR87_RS10870 0.46 -0.2 2,302,423 - ABZR87_RS10870 0.46 -0.2 2,302,431 + ABZR87_RS10870 0.47 +0.0 2,302,432 - ABZR87_RS10870 0.47 -0.1 2,302,432 - ABZR87_RS10870 0.47 +0.6 2,302,432 - ABZR87_RS10870 0.47 -1.3 2,302,709 - ABZR87_RS10870 0.80 -0.3 2,302,785 + ABZR87_RS10870 0.89 -0.2 2,302,833 + -1.8 2,302,855 + +1.4 2,302,870 + +0.8 2,302,870 + -1.0 2,302,871 - -1.0 2,302,942 + -1.1 2,302,942 + -1.1 2,302,942 + -0.3 2,302,942 + -0.4 2,302,943 - -1.0 2,302,943 - +0.1 2,302,943 - -0.7 2,303,010 - ABZR87_RS10875 0.11 -0.5 2,303,010 - ABZR87_RS10875 0.11 -1.7 2,303,010 - ABZR87_RS10875 0.11 +0.4 2,303,010 - ABZR87_RS10875 0.11 -0.4 2,303,047 + ABZR87_RS10875 0.15 -0.8 2,303,047 + ABZR87_RS10875 0.15 -1.0 2,303,048 - ABZR87_RS10875 0.15 -0.4 2,303,048 - ABZR87_RS10875 0.15 -1.3 2,303,205 + ABZR87_RS10875 0.28 -0.7 2,303,206 - ABZR87_RS10875 0.29 -1.3 2,303,206 - ABZR87_RS10875 0.29 +0.4 2,303,457 + ABZR87_RS10875 0.51 -0.2 2,303,457 + ABZR87_RS10875 0.51 -1.3 2,303,457 + ABZR87_RS10875 0.51 -0.7 2,303,457 + ABZR87_RS10875 0.51 -1.7 2,303,457 + ABZR87_RS10875 0.51 -1.2 2,303,457 + ABZR87_RS10875 0.51 -0.9 2,303,457 + ABZR87_RS10875 0.51 -1.1 2,303,457 + ABZR87_RS10875 0.51 +2.0 2,303,458 - ABZR87_RS10875 0.51 +0.3 2,303,458 - ABZR87_RS10875 0.51 -0.4 2,303,458 - ABZR87_RS10875 0.51 -0.2 2,303,458 - ABZR87_RS10875 0.51 -1.5 2,303,458 - ABZR87_RS10875 0.51 -0.3 2,303,458 - ABZR87_RS10875 0.51 +0.1 2,303,458 - ABZR87_RS10875 0.51 -1.1 2,303,458 - ABZR87_RS10875 0.51 -0.4 2,303,458 - ABZR87_RS10875 0.51 -1.0 2,303,458 - ABZR87_RS10875 0.51 -0.8 2,303,514 - ABZR87_RS10875 0.56 -1.6 2,303,514 - ABZR87_RS10875 0.56 +0.0 2,303,514 - ABZR87_RS10875 0.56 -0.0 2,303,699 + ABZR87_RS10875 0.72 +0.1 2,303,699 + ABZR87_RS10875 0.72 -0.4 2,303,844 + ABZR87_RS10875 0.85 -0.3 2,303,844 + ABZR87_RS10875 0.85 -0.5 2,303,845 - ABZR87_RS10875 0.85 -0.4 2,303,845 - ABZR87_RS10875 0.85 +2.0 2,303,845 - ABZR87_RS10875 0.85 -0.3 2,303,845 - ABZR87_RS10875 0.85 -0.4 2,303,848 + ABZR87_RS10875 0.85 -1.7 2,303,849 - ABZR87_RS10875 0.85 -0.6 2,303,849 - ABZR87_RS10875 0.85 -0.6 2,303,874 + ABZR87_RS10875 0.87 -0.9 2,303,874 + ABZR87_RS10875 0.87 -0.2 2,303,874 + ABZR87_RS10875 0.87 -0.2 2,303,874 + ABZR87_RS10875 0.87 -1.0 2,303,875 - ABZR87_RS10875 0.87 -1.4 2,303,875 - ABZR87_RS10875 0.87 +0.0 2,303,875 - ABZR87_RS10875 0.87 -0.9 2,303,875 - ABZR87_RS10875 0.87 -0.4 2,303,875 - ABZR87_RS10875 0.87 -1.2 2,303,875 - ABZR87_RS10875 0.87 +0.4 2,303,875 - ABZR87_RS10875 0.87 -1.1 2,303,875 - ABZR87_RS10875 0.87 -0.3 2,304,000 - -0.2 2,304,016 - -0.3 2,304,176 + ABZR87_RS10880 0.14 -1.0 2,304,265 + ABZR87_RS10880 0.24 -0.3 2,304,265 + ABZR87_RS10880 0.24 +0.9 2,304,265 + ABZR87_RS10880 0.24 +0.3 2,304,265 + ABZR87_RS10880 0.24 -0.0 2,304,266 - ABZR87_RS10880 0.24 -0.4 2,304,266 - ABZR87_RS10880 0.24 -0.3 2,304,266 - ABZR87_RS10880 0.24 -0.1 2,304,491 + ABZR87_RS10880 0.50 +0.8 2,304,492 - ABZR87_RS10880 0.51 -0.5 2,304,494 + ABZR87_RS10880 0.51 +0.0 2,304,495 - ABZR87_RS10880 0.51 +0.6 2,304,495 - ABZR87_RS10880 0.51 -0.1 2,304,570 + ABZR87_RS10880 0.60 -0.4 2,304,570 + ABZR87_RS10880 0.60 +0.3 2,304,570 + ABZR87_RS10880 0.60 -0.3 2,304,570 + ABZR87_RS10880 0.60 -1.3 2,304,571 - ABZR87_RS10880 0.60 +0.2 2,304,571 - ABZR87_RS10880 0.60 -0.7 2,304,571 - ABZR87_RS10880 0.60 -0.6 2,304,572 + ABZR87_RS10880 0.60 +0.1 2,304,572 + ABZR87_RS10880 0.60 -0.5 2,304,572 + ABZR87_RS10880 0.60 -0.2 2,304,572 + ABZR87_RS10880 0.60 -0.7 2,304,572 + ABZR87_RS10880 0.60 -2.9 2,304,572 + ABZR87_RS10880 0.60 -1.6 2,304,572 + ABZR87_RS10880 0.60 -0.1 2,304,572 + ABZR87_RS10880 0.60 +0.0 2,304,572 + ABZR87_RS10880 0.60 -0.6 2,304,572 + ABZR87_RS10880 0.60 -1.3 2,304,572 + ABZR87_RS10880 0.60 +1.1 2,304,572 + ABZR87_RS10880 0.60 -0.2 2,304,572 + ABZR87_RS10880 0.60 -0.0 2,304,572 + ABZR87_RS10880 0.60 +0.6 2,304,573 - ABZR87_RS10880 0.60 -0.8 2,304,573 - ABZR87_RS10880 0.60 +1.2 2,304,573 - ABZR87_RS10880 0.60 +0.1 2,304,573 - ABZR87_RS10880 0.60 +1.3 2,304,573 - ABZR87_RS10880 0.60 +0.6 2,304,573 - ABZR87_RS10880 0.60 +0.4 2,304,573 - ABZR87_RS10880 0.60 +0.4 2,304,573 - ABZR87_RS10880 0.60 -0.2 2,304,573 - ABZR87_RS10880 0.60 -0.8 2,304,573 - ABZR87_RS10880 0.60 +0.1 2,304,573 - ABZR87_RS10880 0.60 -0.4 2,304,573 - ABZR87_RS10880 0.60 -0.2 2,304,573 - ABZR87_RS10880 0.60 -0.7 2,304,573 - ABZR87_RS10880 0.60 -0.0 2,304,573 - ABZR87_RS10880 0.60 +0.6 2,304,573 - ABZR87_RS10880 0.60 +0.3 2,304,573 - ABZR87_RS10880 0.60 +1.4 2,304,573 - ABZR87_RS10880 0.60 +0.7 2,304,598 + ABZR87_RS10880 0.63 -2.4 2,304,599 - ABZR87_RS10880 0.63 +0.0 2,304,599 - ABZR87_RS10880 0.63 -1.1 2,304,599 - ABZR87_RS10880 0.63 +0.2 2,304,599 - ABZR87_RS10880 0.63 -0.1 2,304,710 + ABZR87_RS10880 0.76 -0.4 2,304,710 + ABZR87_RS10880 0.76 -1.3 2,304,710 + ABZR87_RS10880 0.76 -1.0 2,304,711 - ABZR87_RS10880 0.76 -0.1 2,304,711 - ABZR87_RS10880 0.76 +1.2 2,304,937 + -0.2 2,304,937 + +0.5 2,304,937 + +0.7 2,304,937 + -1.4 2,304,937 + +0.2 2,304,937 + +1.3 2,304,937 + +0.2 2,304,937 + -1.8 2,304,937 + -0.0 2,304,938 - -0.3 2,304,938 - +0.3 2,304,938 - -1.1 2,304,938 - +0.6 2,304,938 - -0.9 2,304,938 - -0.9 2,304,994 + +0.0 2,304,995 - -1.6 2,304,995 - +0.1 2,304,995 - +0.3 2,304,995 - -0.6
Or see this region's nucleotide sequence