Strain Fitness in Ralstonia sp. UNC404CL21Col around ABZR87_RS10530

Experiment: Acanthamoeba sp., muxed culture-2

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Acanthamoeba sp., muxed culture-2
2,227,256	-		ABZR87_RS10520	0.71	+0.1
2,227,337	+		ABZR87_RS10520	0.81	+0.5
2,227,337	+		ABZR87_RS10520	0.81	+0.7
2,227,338	-		ABZR87_RS10520	0.81	+0.5
2,227,338	-		ABZR87_RS10520	0.81	+0.7
2,227,338	-		ABZR87_RS10520	0.81	+0.2
2,227,350	+		ABZR87_RS10520	0.82	+0.3
2,227,350	+		ABZR87_RS10520	0.82	+0.6
2,227,351	-		ABZR87_RS10520	0.82	+1.2
2,227,359	+		ABZR87_RS10520	0.83	+0.3
2,227,360	-		ABZR87_RS10520	0.83	+0.1
2,227,360	-		ABZR87_RS10520	0.83	+0.6
2,227,360	-		ABZR87_RS10520	0.83	+0.1
2,228,059	-		ABZR87_RS10525	0.87	+1.1
2,228,059	-		ABZR87_RS10525	0.87	+1.5
2,228,137	+				-0.4
2,228,137	+				-0.9
2,228,137	+				+0.6
2,228,138	-				-0.2
2,228,138	-				-0.2
2,228,160	+				+0.6
2,228,191	+				-0.1
2,228,191	+				+0.2
2,228,192	-				+0.2
2,228,192	-				-1.4
2,228,192	-				+0.0
2,228,192	-				-0.3
2,228,192	-				-0.1
2,228,196	+				+1.6
2,228,323	+		ABZR87_RS10530	0.18	-0.1
2,228,323	+		ABZR87_RS10530	0.18	+0.7
2,228,401	+		ABZR87_RS10530	0.29	-0.6
2,228,401	+		ABZR87_RS10530	0.29	+0.0
2,228,628	+		ABZR87_RS10530	0.63	+1.5
2,228,628	+		ABZR87_RS10530	0.63	+1.5
2,228,628	+		ABZR87_RS10530	0.63	+0.3
2,228,732	+		ABZR87_RS10530	0.79	-0.3
2,228,732	+		ABZR87_RS10530	0.79	+0.0
2,228,732	+		ABZR87_RS10530	0.79	-0.9
2,228,732	+		ABZR87_RS10530	0.79	+1.0
2,228,732	+		ABZR87_RS10530	0.79	+0.1
2,228,732	+		ABZR87_RS10530	0.79	-0.3
2,228,784	+		ABZR87_RS10530	0.87	-1.2