Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS10165 and ABZR87_RS10170 are separated by 107 nucleotides ABZR87_RS10170 and ABZR87_RS10175 are separated by 11 nucleotides ABZR87_RS10175 and ABZR87_RS10180 are separated by 85 nucleotides
ABZR87_RS10165: ABZR87_RS10165 - RNA polymerase sigma factor RpoE, at 2,157,414 to 2,158,013
_RS10165
ABZR87_RS10170: ABZR87_RS10170 - RseA family anti-sigma factor, at 2,158,121 to 2,158,753
_RS10170
ABZR87_RS10175: ABZR87_RS10175 - MucB/RseB C-terminal domain-containing protein, at 2,158,765 to 2,159,862
_RS10175
ABZR87_RS10180: ABZR87_RS10180 - DegQ family serine endoprotease, at 2,159,948 to 2,161,459
_RS10180
Position (kb)
2158
2159
2160 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2157.767 kb on + strand, within ABZR87_RS10165 at 2157.767 kb on + strand, within ABZR87_RS10165 at 2157.768 kb on - strand, within ABZR87_RS10165 at 2157.768 kb on - strand, within ABZR87_RS10165 at 2157.768 kb on - strand, within ABZR87_RS10165 at 2157.885 kb on + strand, within ABZR87_RS10165 at 2157.885 kb on + strand, within ABZR87_RS10165 at 2157.885 kb on + strand, within ABZR87_RS10165 at 2157.885 kb on + strand, within ABZR87_RS10165 at 2157.886 kb on - strand, within ABZR87_RS10165 at 2157.886 kb on - strand, within ABZR87_RS10165 at 2157.886 kb on - strand, within ABZR87_RS10165 at 2157.886 kb on - strand, within ABZR87_RS10165 at 2158.309 kb on + strand, within ABZR87_RS10170 at 2158.309 kb on + strand, within ABZR87_RS10170 at 2158.309 kb on + strand, within ABZR87_RS10170 at 2158.310 kb on - strand, within ABZR87_RS10170 at 2158.310 kb on - strand, within ABZR87_RS10170 at 2158.310 kb on - strand, within ABZR87_RS10170 at 2158.492 kb on + strand, within ABZR87_RS10170 at 2158.493 kb on - strand, within ABZR87_RS10170 at 2158.650 kb on - strand, within ABZR87_RS10170 at 2158.650 kb on - strand, within ABZR87_RS10170 at 2158.684 kb on + strand, within ABZR87_RS10170 at 2158.684 kb on + strand, within ABZR87_RS10170 at 2158.685 kb on - strand, within ABZR87_RS10170 at 2158.698 kb on - strand at 2158.752 kb on - strand at 2158.954 kb on + strand, within ABZR87_RS10175 at 2158.954 kb on + strand, within ABZR87_RS10175 at 2158.955 kb on - strand, within ABZR87_RS10175 at 2158.955 kb on - strand, within ABZR87_RS10175 at 2158.972 kb on + strand, within ABZR87_RS10175 at 2158.972 kb on + strand, within ABZR87_RS10175 at 2158.973 kb on - strand, within ABZR87_RS10175 at 2159.113 kb on + strand, within ABZR87_RS10175 at 2159.171 kb on + strand, within ABZR87_RS10175 at 2159.178 kb on + strand, within ABZR87_RS10175 at 2159.206 kb on + strand, within ABZR87_RS10175 at 2159.296 kb on + strand, within ABZR87_RS10175 at 2159.296 kb on + strand, within ABZR87_RS10175 at 2159.301 kb on + strand, within ABZR87_RS10175 at 2159.316 kb on + strand, within ABZR87_RS10175 at 2159.316 kb on + strand, within ABZR87_RS10175 at 2159.317 kb on - strand, within ABZR87_RS10175 at 2159.464 kb on + strand, within ABZR87_RS10175 at 2159.465 kb on - strand, within ABZR87_RS10175 at 2159.759 kb on + strand at 2159.809 kb on + strand at 2159.809 kb on + strand at 2159.810 kb on - strand at 2159.838 kb on + strand at 2159.838 kb on + strand at 2159.839 kb on - strand at 2159.839 kb on - strand at 2159.861 kb on - strand at 2159.861 kb on - strand at 2159.922 kb on - strand at 2159.953 kb on + strand at 2159.954 kb on - strand at 2160.071 kb on + strand at 2160.071 kb on + strand at 2160.071 kb on + strand at 2160.071 kb on + strand at 2160.071 kb on + strand at 2160.072 kb on - strand at 2160.072 kb on - strand at 2160.072 kb on - strand at 2160.072 kb on - strand at 2160.072 kb on - strand at 2160.151 kb on + strand, within ABZR87_RS10180 at 2160.151 kb on + strand, within ABZR87_RS10180 at 2160.152 kb on - strand, within ABZR87_RS10180 at 2160.152 kb on - strand, within ABZR87_RS10180 at 2160.326 kb on + strand, within ABZR87_RS10180 at 2160.326 kb on + strand, within ABZR87_RS10180 at 2160.326 kb on + strand, within ABZR87_RS10180 at 2160.327 kb on - strand, within ABZR87_RS10180 at 2160.327 kb on - strand, within ABZR87_RS10180 at 2160.327 kb on - strand, within ABZR87_RS10180 at 2160.327 kb on - strand, within ABZR87_RS10180 at 2160.327 kb on - strand, within ABZR87_RS10180 at 2160.371 kb on + strand, within ABZR87_RS10180 at 2160.371 kb on + strand, within ABZR87_RS10180 at 2160.371 kb on + strand, within ABZR87_RS10180 at 2160.371 kb on + strand, within ABZR87_RS10180 at 2160.372 kb on - strand, within ABZR87_RS10180 at 2160.372 kb on - strand, within ABZR87_RS10180 at 2160.372 kb on - strand, within ABZR87_RS10180 at 2160.372 kb on - strand, within ABZR87_RS10180 at 2160.553 kb on + strand, within ABZR87_RS10180 at 2160.553 kb on + strand, within ABZR87_RS10180 at 2160.554 kb on - strand, within ABZR87_RS10180 at 2160.554 kb on - strand, within ABZR87_RS10180 at 2160.554 kb on - strand, within ABZR87_RS10180 at 2160.603 kb on - strand, within ABZR87_RS10180 at 2160.704 kb on + strand, within ABZR87_RS10180 at 2160.704 kb on + strand, within ABZR87_RS10180 at 2160.704 kb on + strand, within ABZR87_RS10180 at 2160.704 kb on + strand, within ABZR87_RS10180 at 2160.704 kb on + strand, within ABZR87_RS10180 at 2160.704 kb on + strand, within ABZR87_RS10180 at 2160.705 kb on - strand, within ABZR87_RS10180 at 2160.705 kb on - strand, within ABZR87_RS10180 at 2160.705 kb on - strand, within ABZR87_RS10180 at 2160.705 kb on - strand, within ABZR87_RS10180 at 2160.705 kb on - strand, within ABZR87_RS10180 at 2160.705 kb on - strand, within ABZR87_RS10180 at 2160.722 kb on + strand, within ABZR87_RS10180 at 2160.722 kb on + strand, within ABZR87_RS10180 at 2160.723 kb on - strand, within ABZR87_RS10180
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 2,157,767 + ABZR87_RS10165 0.59 +1.2 2,157,767 + ABZR87_RS10165 0.59 +2.5 2,157,768 - ABZR87_RS10165 0.59 +2.1 2,157,768 - ABZR87_RS10165 0.59 +1.0 2,157,768 - ABZR87_RS10165 0.59 +1.9 2,157,885 + ABZR87_RS10165 0.79 +1.3 2,157,885 + ABZR87_RS10165 0.79 +0.4 2,157,885 + ABZR87_RS10165 0.79 +0.1 2,157,885 + ABZR87_RS10165 0.79 +1.8 2,157,886 - ABZR87_RS10165 0.79 +1.5 2,157,886 - ABZR87_RS10165 0.79 +1.7 2,157,886 - ABZR87_RS10165 0.79 +1.6 2,157,886 - ABZR87_RS10165 0.79 +1.6 2,158,309 + ABZR87_RS10170 0.30 -0.9 2,158,309 + ABZR87_RS10170 0.30 -1.0 2,158,309 + ABZR87_RS10170 0.30 +0.8 2,158,310 - ABZR87_RS10170 0.30 +0.1 2,158,310 - ABZR87_RS10170 0.30 +0.0 2,158,310 - ABZR87_RS10170 0.30 +1.8 2,158,492 + ABZR87_RS10170 0.59 -0.2 2,158,493 - ABZR87_RS10170 0.59 -0.9 2,158,650 - ABZR87_RS10170 0.84 +0.5 2,158,650 - ABZR87_RS10170 0.84 -1.1 2,158,684 + ABZR87_RS10170 0.89 +0.6 2,158,684 + ABZR87_RS10170 0.89 +0.9 2,158,685 - ABZR87_RS10170 0.89 +0.5 2,158,698 - -1.3 2,158,752 - -0.4 2,158,954 + ABZR87_RS10175 0.17 -1.3 2,158,954 + ABZR87_RS10175 0.17 -2.5 2,158,955 - ABZR87_RS10175 0.17 -0.6 2,158,955 - ABZR87_RS10175 0.17 -0.5 2,158,972 + ABZR87_RS10175 0.19 -1.5 2,158,972 + ABZR87_RS10175 0.19 -1.4 2,158,973 - ABZR87_RS10175 0.19 -0.3 2,159,113 + ABZR87_RS10175 0.32 -1.0 2,159,171 + ABZR87_RS10175 0.37 -2.3 2,159,178 + ABZR87_RS10175 0.38 +1.3 2,159,206 + ABZR87_RS10175 0.40 +0.0 2,159,296 + ABZR87_RS10175 0.48 +0.3 2,159,296 + ABZR87_RS10175 0.48 +0.0 2,159,301 + ABZR87_RS10175 0.49 -0.5 2,159,316 + ABZR87_RS10175 0.50 -1.0 2,159,316 + ABZR87_RS10175 0.50 -1.1 2,159,317 - ABZR87_RS10175 0.50 -0.9 2,159,464 + ABZR87_RS10175 0.64 +1.2 2,159,465 - ABZR87_RS10175 0.64 -0.5 2,159,759 + -0.5 2,159,809 + +0.8 2,159,809 + -1.3 2,159,810 - +0.4 2,159,838 + +0.5 2,159,838 + +0.9 2,159,839 - +0.1 2,159,839 - -0.4 2,159,861 - +0.4 2,159,861 - +0.4 2,159,922 - -1.0 2,159,953 + +0.5 2,159,954 - -1.1 2,160,071 + -0.7 2,160,071 + -1.0 2,160,071 + +0.2 2,160,071 + +2.0 2,160,071 + -0.0 2,160,072 - +0.0 2,160,072 - +0.0 2,160,072 - -1.0 2,160,072 - -0.1 2,160,072 - +0.5 2,160,151 + ABZR87_RS10180 0.13 -1.6 2,160,151 + ABZR87_RS10180 0.13 -0.8 2,160,152 - ABZR87_RS10180 0.13 -1.4 2,160,152 - ABZR87_RS10180 0.13 +1.2 2,160,326 + ABZR87_RS10180 0.25 +1.7 2,160,326 + ABZR87_RS10180 0.25 +0.9 2,160,326 + ABZR87_RS10180 0.25 -0.3 2,160,327 - ABZR87_RS10180 0.25 -0.8 2,160,327 - ABZR87_RS10180 0.25 +0.2 2,160,327 - ABZR87_RS10180 0.25 +0.6 2,160,327 - ABZR87_RS10180 0.25 -0.1 2,160,327 - ABZR87_RS10180 0.25 -1.4 2,160,371 + ABZR87_RS10180 0.28 -0.5 2,160,371 + ABZR87_RS10180 0.28 -0.0 2,160,371 + ABZR87_RS10180 0.28 -1.2 2,160,371 + ABZR87_RS10180 0.28 +0.4 2,160,372 - ABZR87_RS10180 0.28 +0.0 2,160,372 - ABZR87_RS10180 0.28 +0.3 2,160,372 - ABZR87_RS10180 0.28 -1.7 2,160,372 - ABZR87_RS10180 0.28 +0.1 2,160,553 + ABZR87_RS10180 0.40 -0.3 2,160,553 + ABZR87_RS10180 0.40 +0.4 2,160,554 - ABZR87_RS10180 0.40 +0.1 2,160,554 - ABZR87_RS10180 0.40 +0.4 2,160,554 - ABZR87_RS10180 0.40 +0.4 2,160,603 - ABZR87_RS10180 0.43 +0.5 2,160,704 + ABZR87_RS10180 0.50 +0.6 2,160,704 + ABZR87_RS10180 0.50 +0.4 2,160,704 + ABZR87_RS10180 0.50 +0.5 2,160,704 + ABZR87_RS10180 0.50 -0.9 2,160,704 + ABZR87_RS10180 0.50 -1.0 2,160,704 + ABZR87_RS10180 0.50 -2.0 2,160,705 - ABZR87_RS10180 0.50 -0.1 2,160,705 - ABZR87_RS10180 0.50 -3.0 2,160,705 - ABZR87_RS10180 0.50 -0.3 2,160,705 - ABZR87_RS10180 0.50 -0.3 2,160,705 - ABZR87_RS10180 0.50 +1.8 2,160,705 - ABZR87_RS10180 0.50 -0.9 2,160,722 + ABZR87_RS10180 0.51 +1.3 2,160,722 + ABZR87_RS10180 0.51 +0.1 2,160,723 - ABZR87_RS10180 0.51 -0.4
Or see this region's nucleotide sequence