Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS10005 and ABZR87_RS10010 are separated by 52 nucleotides ABZR87_RS10010 and ABZR87_RS10015 are separated by 31 nucleotides ABZR87_RS10015 and ABZR87_RS10020 are separated by 82 nucleotides
ABZR87_RS10005: ABZR87_RS10005 - Fe-S protein assembly chaperone HscA, at 2,124,683 to 2,126,548
_RS10005
ABZR87_RS10010: ABZR87_RS10010 - ISC system 2Fe-2S type ferredoxin, at 2,126,601 to 2,126,939
_RS10010
ABZR87_RS10015: ABZR87_RS10015 - Fe-S cluster assembly protein IscX, at 2,126,971 to 2,127,165
_RS10015
ABZR87_RS10020: ABZR87_RS10020 - glycine zipper 2TM domain-containing protein, at 2,127,248 to 2,127,991
_RS10020
Position (kb)
2126
2127 Strain fitness (log2 ratio)
-1
0
1 at 2126.530 kb on + strand at 2126.583 kb on + strand at 2126.583 kb on + strand at 2127.011 kb on - strand, within ABZR87_RS10015 at 2127.019 kb on + strand, within ABZR87_RS10015 at 2127.019 kb on + strand, within ABZR87_RS10015 at 2127.020 kb on - strand, within ABZR87_RS10015 at 2127.020 kb on - strand, within ABZR87_RS10015 at 2127.164 kb on - strand at 2127.171 kb on + strand at 2127.171 kb on + strand at 2127.171 kb on + strand at 2127.318 kb on + strand at 2127.319 kb on - strand at 2127.337 kb on + strand, within ABZR87_RS10020 at 2127.338 kb on - strand, within ABZR87_RS10020 at 2127.388 kb on - strand, within ABZR87_RS10020 at 2127.388 kb on - strand, within ABZR87_RS10020 at 2127.388 kb on - strand, within ABZR87_RS10020 at 2127.423 kb on - strand, within ABZR87_RS10020 at 2127.448 kb on + strand, within ABZR87_RS10020 at 2127.609 kb on + strand, within ABZR87_RS10020 at 2127.642 kb on + strand, within ABZR87_RS10020 at 2127.642 kb on + strand, within ABZR87_RS10020
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 2,126,530 + -0.3 2,126,583 + -0.4 2,126,583 + -0.9 2,127,011 - ABZR87_RS10015 0.21 -0.8 2,127,019 + ABZR87_RS10015 0.25 -0.5 2,127,019 + ABZR87_RS10015 0.25 -0.0 2,127,020 - ABZR87_RS10015 0.25 +1.4 2,127,020 - ABZR87_RS10015 0.25 +1.3 2,127,164 - -0.5 2,127,171 + -1.0 2,127,171 + +0.6 2,127,171 + -0.8 2,127,318 + -0.4 2,127,319 - -1.4 2,127,337 + ABZR87_RS10020 0.12 -1.1 2,127,338 - ABZR87_RS10020 0.12 -1.5 2,127,388 - ABZR87_RS10020 0.19 -0.0 2,127,388 - ABZR87_RS10020 0.19 -0.1 2,127,388 - ABZR87_RS10020 0.19 -0.1 2,127,423 - ABZR87_RS10020 0.24 -0.1 2,127,448 + ABZR87_RS10020 0.27 -1.7 2,127,609 + ABZR87_RS10020 0.49 +0.8 2,127,642 + ABZR87_RS10020 0.53 +0.2 2,127,642 + ABZR87_RS10020 0.53 +0.5
Or see this region's nucleotide sequence