Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS09840 and ABZR87_RS09845 are separated by 8 nucleotides ABZR87_RS09845 and ABZR87_RS09850 are separated by 163 nucleotides ABZR87_RS09850 and ABZR87_RS09855 are separated by 214 nucleotides ABZR87_RS09855 and ABZR87_RS09860 are separated by 8 nucleotides
ABZR87_RS09840: ABZR87_RS09840 - molecular chaperone HtpG, at 2,091,283 to 2,093,205
_RS09840
ABZR87_RS09845: ABZR87_RS09845 - DNA-deoxyinosine glycosylase, at 2,093,214 to 2,093,711
_RS09845
ABZR87_RS09850: ABZR87_RS09850 - DUF1993 family protein, at 2,093,875 to 2,094,384
_RS09850
ABZR87_RS09855: ABZR87_RS09855 - tetratricopeptide repeat protein, at 2,094,599 to 2,095,357
_RS09855
ABZR87_RS09860: ABZR87_RS09860 - spermidine synthase, at 2,095,366 to 2,096,241
_RS09860
Position (kb)
2093
2094
2095 Strain fitness (log2 ratio)
-2
-1
0
1 at 2092.947 kb on + strand, within ABZR87_RS09840 at 2093.174 kb on - strand at 2093.316 kb on + strand, within ABZR87_RS09845 at 2093.316 kb on + strand, within ABZR87_RS09845 at 2093.317 kb on - strand, within ABZR87_RS09845 at 2093.317 kb on - strand, within ABZR87_RS09845 at 2093.322 kb on + strand, within ABZR87_RS09845 at 2093.323 kb on - strand, within ABZR87_RS09845 at 2093.388 kb on + strand, within ABZR87_RS09845 at 2093.646 kb on + strand, within ABZR87_RS09845 at 2093.646 kb on + strand, within ABZR87_RS09845 at 2093.646 kb on + strand, within ABZR87_RS09845 at 2093.647 kb on - strand, within ABZR87_RS09845 at 2093.661 kb on + strand, within ABZR87_RS09845 at 2093.662 kb on - strand at 2093.709 kb on + strand at 2093.734 kb on + strand at 2093.741 kb on + strand at 2093.794 kb on + strand at 2093.794 kb on + strand at 2093.794 kb on + strand at 2093.794 kb on + strand at 2093.794 kb on + strand at 2093.794 kb on + strand at 2093.795 kb on - strand at 2093.893 kb on + strand at 2093.893 kb on + strand at 2093.893 kb on + strand at 2093.893 kb on + strand at 2093.894 kb on - strand at 2093.965 kb on + strand, within ABZR87_RS09850 at 2093.966 kb on - strand, within ABZR87_RS09850 at 2094.271 kb on + strand, within ABZR87_RS09850 at 2094.271 kb on + strand, within ABZR87_RS09850 at 2094.272 kb on - strand, within ABZR87_RS09850 at 2094.272 kb on - strand, within ABZR87_RS09850 at 2094.301 kb on + strand, within ABZR87_RS09850 at 2094.302 kb on - strand, within ABZR87_RS09850 at 2094.680 kb on + strand, within ABZR87_RS09855 at 2094.680 kb on + strand, within ABZR87_RS09855 at 2094.680 kb on + strand, within ABZR87_RS09855 at 2094.681 kb on - strand, within ABZR87_RS09855 at 2094.681 kb on - strand, within ABZR87_RS09855 at 2094.743 kb on + strand, within ABZR87_RS09855 at 2094.744 kb on - strand, within ABZR87_RS09855 at 2095.097 kb on + strand, within ABZR87_RS09855 at 2095.097 kb on + strand, within ABZR87_RS09855 at 2095.097 kb on + strand, within ABZR87_RS09855 at 2095.098 kb on - strand, within ABZR87_RS09855 at 2095.098 kb on - strand, within ABZR87_RS09855 at 2095.098 kb on - strand, within ABZR87_RS09855 at 2095.098 kb on - strand, within ABZR87_RS09855 at 2095.365 kb on + strand at 2095.384 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 2,092,947 + ABZR87_RS09840 0.87 +0.3 2,093,174 - +0.6 2,093,316 + ABZR87_RS09845 0.20 +0.0 2,093,316 + ABZR87_RS09845 0.20 +0.2 2,093,317 - ABZR87_RS09845 0.21 +0.9 2,093,317 - ABZR87_RS09845 0.21 -0.6 2,093,322 + ABZR87_RS09845 0.22 +0.8 2,093,323 - ABZR87_RS09845 0.22 +0.5 2,093,388 + ABZR87_RS09845 0.35 +0.6 2,093,646 + ABZR87_RS09845 0.87 +0.8 2,093,646 + ABZR87_RS09845 0.87 +0.4 2,093,646 + ABZR87_RS09845 0.87 -0.5 2,093,647 - ABZR87_RS09845 0.87 -0.5 2,093,661 + ABZR87_RS09845 0.90 -0.2 2,093,662 - +0.0 2,093,709 + +0.4 2,093,734 + +0.2 2,093,741 + +1.2 2,093,794 + -0.0 2,093,794 + -0.2 2,093,794 + +0.0 2,093,794 + -1.6 2,093,794 + -0.5 2,093,794 + +1.0 2,093,795 - +1.0 2,093,893 + +0.1 2,093,893 + -2.4 2,093,893 + -1.5 2,093,893 + +0.0 2,093,894 - -0.6 2,093,965 + ABZR87_RS09850 0.18 -1.4 2,093,966 - ABZR87_RS09850 0.18 -1.0 2,094,271 + ABZR87_RS09850 0.78 -0.2 2,094,271 + ABZR87_RS09850 0.78 -0.2 2,094,272 - ABZR87_RS09850 0.78 -0.7 2,094,272 - ABZR87_RS09850 0.78 -1.4 2,094,301 + ABZR87_RS09850 0.84 +0.2 2,094,302 - ABZR87_RS09850 0.84 +0.0 2,094,680 + ABZR87_RS09855 0.11 +0.2 2,094,680 + ABZR87_RS09855 0.11 -0.2 2,094,680 + ABZR87_RS09855 0.11 -1.3 2,094,681 - ABZR87_RS09855 0.11 -1.9 2,094,681 - ABZR87_RS09855 0.11 +0.7 2,094,743 + ABZR87_RS09855 0.19 -0.3 2,094,744 - ABZR87_RS09855 0.19 +1.3 2,095,097 + ABZR87_RS09855 0.66 +0.5 2,095,097 + ABZR87_RS09855 0.66 -0.1 2,095,097 + ABZR87_RS09855 0.66 +0.1 2,095,098 - ABZR87_RS09855 0.66 +0.4 2,095,098 - ABZR87_RS09855 0.66 -1.1 2,095,098 - ABZR87_RS09855 0.66 +0.2 2,095,098 - ABZR87_RS09855 0.66 -0.5 2,095,365 + +0.3 2,095,384 - +0.2
Or see this region's nucleotide sequence