Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS09820 and ABZR87_RS09825 are separated by 45 nucleotides ABZR87_RS09825 and ABZR87_RS09830 are separated by 9 nucleotides ABZR87_RS09830 and ABZR87_RS09835 are separated by 131 nucleotides
ABZR87_RS09820: ABZR87_RS09820 - RidA family protein, at 2,087,387 to 2,087,857
_RS09820
ABZR87_RS09825: ABZR87_RS09825 - DMT family transporter, at 2,087,903 to 2,088,841
_RS09825
ABZR87_RS09830: ABZR87_RS09830 - alanyl-tRNA editing protein, at 2,088,851 to 2,089,570
_RS09830
ABZR87_RS09835: ABZR87_RS09835 - PLP-dependent aminotransferase family protein, at 2,089,702 to 2,091,180
_RS09835
Position (kb)
2088
2089
2090 Strain fitness (log2 ratio)
-2
-1
0
1 at 2087.871 kb on + strand at 2087.871 kb on + strand at 2087.871 kb on + strand at 2087.871 kb on + strand at 2087.872 kb on - strand at 2087.872 kb on - strand at 2087.872 kb on - strand at 2087.905 kb on - strand at 2088.041 kb on + strand, within ABZR87_RS09825 at 2088.041 kb on + strand, within ABZR87_RS09825 at 2088.041 kb on + strand, within ABZR87_RS09825 at 2088.042 kb on - strand, within ABZR87_RS09825 at 2088.084 kb on + strand, within ABZR87_RS09825 at 2088.085 kb on - strand, within ABZR87_RS09825 at 2088.122 kb on + strand, within ABZR87_RS09825 at 2088.122 kb on + strand, within ABZR87_RS09825 at 2088.123 kb on - strand, within ABZR87_RS09825 at 2088.126 kb on + strand, within ABZR87_RS09825 at 2088.126 kb on + strand, within ABZR87_RS09825 at 2088.126 kb on + strand, within ABZR87_RS09825 at 2088.127 kb on - strand, within ABZR87_RS09825 at 2088.127 kb on - strand, within ABZR87_RS09825 at 2088.127 kb on - strand, within ABZR87_RS09825 at 2088.127 kb on - strand, within ABZR87_RS09825 at 2088.127 kb on - strand, within ABZR87_RS09825 at 2088.127 kb on - strand, within ABZR87_RS09825 at 2088.127 kb on - strand, within ABZR87_RS09825 at 2088.127 kb on - strand, within ABZR87_RS09825 at 2088.127 kb on - strand, within ABZR87_RS09825 at 2089.199 kb on - strand, within ABZR87_RS09830 at 2089.199 kb on - strand, within ABZR87_RS09830 at 2089.252 kb on + strand, within ABZR87_RS09830 at 2089.252 kb on + strand, within ABZR87_RS09830 at 2089.252 kb on + strand, within ABZR87_RS09830 at 2089.252 kb on + strand, within ABZR87_RS09830 at 2089.252 kb on + strand, within ABZR87_RS09830 at 2089.253 kb on - strand, within ABZR87_RS09830 at 2089.253 kb on - strand, within ABZR87_RS09830 at 2089.253 kb on - strand, within ABZR87_RS09830 at 2089.253 kb on - strand, within ABZR87_RS09830 at 2089.253 kb on - strand, within ABZR87_RS09830 at 2089.253 kb on - strand, within ABZR87_RS09830 at 2089.253 kb on - strand, within ABZR87_RS09830 at 2089.253 kb on - strand, within ABZR87_RS09830 at 2089.253 kb on - strand, within ABZR87_RS09830 at 2089.253 kb on - strand, within ABZR87_RS09830 at 2089.380 kb on + strand, within ABZR87_RS09830 at 2089.464 kb on + strand, within ABZR87_RS09830 at 2089.464 kb on + strand, within ABZR87_RS09830 at 2089.536 kb on + strand at 2089.536 kb on + strand at 2089.634 kb on + strand at 2089.634 kb on + strand at 2089.634 kb on + strand at 2089.634 kb on + strand at 2089.635 kb on - strand at 2089.635 kb on - strand at 2089.635 kb on - strand at 2089.639 kb on + strand at 2089.645 kb on + strand at 2089.646 kb on - strand at 2089.646 kb on - strand at 2089.646 kb on - strand at 2089.670 kb on - strand at 2089.693 kb on + strand at 2089.693 kb on + strand at 2089.693 kb on + strand at 2089.694 kb on - strand at 2089.694 kb on - strand at 2089.936 kb on + strand, within ABZR87_RS09835 at 2089.936 kb on + strand, within ABZR87_RS09835 at 2089.936 kb on + strand, within ABZR87_RS09835 at 2089.937 kb on - strand, within ABZR87_RS09835 at 2089.961 kb on - strand, within ABZR87_RS09835 at 2089.961 kb on - strand, within ABZR87_RS09835 at 2089.961 kb on - strand, within ABZR87_RS09835 at 2089.990 kb on + strand, within ABZR87_RS09835 at 2089.990 kb on + strand, within ABZR87_RS09835 at 2089.990 kb on + strand, within ABZR87_RS09835 at 2089.991 kb on - strand, within ABZR87_RS09835 at 2089.991 kb on - strand, within ABZR87_RS09835 at 2089.991 kb on - strand, within ABZR87_RS09835 at 2089.991 kb on - strand, within ABZR87_RS09835 at 2090.452 kb on + strand, within ABZR87_RS09835 at 2090.453 kb on - strand, within ABZR87_RS09835 at 2090.542 kb on - strand, within ABZR87_RS09835
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 2,087,871 + -1.4 2,087,871 + -0.3 2,087,871 + -1.9 2,087,871 + -0.4 2,087,872 - +0.1 2,087,872 - -0.8 2,087,872 - -0.1 2,087,905 - +0.7 2,088,041 + ABZR87_RS09825 0.15 +0.6 2,088,041 + ABZR87_RS09825 0.15 -0.9 2,088,041 + ABZR87_RS09825 0.15 +0.7 2,088,042 - ABZR87_RS09825 0.15 +0.4 2,088,084 + ABZR87_RS09825 0.19 +0.7 2,088,085 - ABZR87_RS09825 0.19 -0.2 2,088,122 + ABZR87_RS09825 0.23 +0.2 2,088,122 + ABZR87_RS09825 0.23 +0.3 2,088,123 - ABZR87_RS09825 0.23 -1.9 2,088,126 + ABZR87_RS09825 0.24 +0.4 2,088,126 + ABZR87_RS09825 0.24 -0.4 2,088,126 + ABZR87_RS09825 0.24 +0.1 2,088,127 - ABZR87_RS09825 0.24 +0.9 2,088,127 - ABZR87_RS09825 0.24 -1.0 2,088,127 - ABZR87_RS09825 0.24 +1.4 2,088,127 - ABZR87_RS09825 0.24 +0.1 2,088,127 - ABZR87_RS09825 0.24 +0.8 2,088,127 - ABZR87_RS09825 0.24 -0.6 2,088,127 - ABZR87_RS09825 0.24 +0.1 2,088,127 - ABZR87_RS09825 0.24 +0.3 2,088,127 - ABZR87_RS09825 0.24 +0.8 2,089,199 - ABZR87_RS09830 0.48 -0.9 2,089,199 - ABZR87_RS09830 0.48 +0.5 2,089,252 + ABZR87_RS09830 0.56 +0.3 2,089,252 + ABZR87_RS09830 0.56 +0.2 2,089,252 + ABZR87_RS09830 0.56 +0.2 2,089,252 + ABZR87_RS09830 0.56 +0.7 2,089,252 + ABZR87_RS09830 0.56 +0.6 2,089,253 - ABZR87_RS09830 0.56 +1.1 2,089,253 - ABZR87_RS09830 0.56 -0.5 2,089,253 - ABZR87_RS09830 0.56 -1.0 2,089,253 - ABZR87_RS09830 0.56 -0.5 2,089,253 - ABZR87_RS09830 0.56 -0.7 2,089,253 - ABZR87_RS09830 0.56 -0.6 2,089,253 - ABZR87_RS09830 0.56 -0.6 2,089,253 - ABZR87_RS09830 0.56 +0.2 2,089,253 - ABZR87_RS09830 0.56 -0.3 2,089,253 - ABZR87_RS09830 0.56 -0.8 2,089,380 + ABZR87_RS09830 0.73 +0.1 2,089,464 + ABZR87_RS09830 0.85 -0.1 2,089,464 + ABZR87_RS09830 0.85 -0.6 2,089,536 + +0.8 2,089,536 + -0.7 2,089,634 + -0.1 2,089,634 + +0.4 2,089,634 + +0.0 2,089,634 + -0.4 2,089,635 - -0.2 2,089,635 - +0.4 2,089,635 - +0.2 2,089,639 + -0.3 2,089,645 + -0.3 2,089,646 - -0.9 2,089,646 - -0.9 2,089,646 - +0.3 2,089,670 - +0.4 2,089,693 + +0.7 2,089,693 + +0.3 2,089,693 + +0.1 2,089,694 - +0.2 2,089,694 - -0.5 2,089,936 + ABZR87_RS09835 0.16 -0.1 2,089,936 + ABZR87_RS09835 0.16 -0.8 2,089,936 + ABZR87_RS09835 0.16 -0.4 2,089,937 - ABZR87_RS09835 0.16 -0.5 2,089,961 - ABZR87_RS09835 0.18 -0.4 2,089,961 - ABZR87_RS09835 0.18 -0.4 2,089,961 - ABZR87_RS09835 0.18 +0.1 2,089,990 + ABZR87_RS09835 0.19 -0.6 2,089,990 + ABZR87_RS09835 0.19 +0.9 2,089,990 + ABZR87_RS09835 0.19 -0.8 2,089,991 - ABZR87_RS09835 0.20 -1.5 2,089,991 - ABZR87_RS09835 0.20 -0.0 2,089,991 - ABZR87_RS09835 0.20 +1.0 2,089,991 - ABZR87_RS09835 0.20 +0.6 2,090,452 + ABZR87_RS09835 0.51 +0.5 2,090,453 - ABZR87_RS09835 0.51 -0.9 2,090,542 - ABZR87_RS09835 0.57 -2.1
Or see this region's nucleotide sequence