Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS09625 and ABZR87_RS09630 are separated by 36 nucleotides ABZR87_RS09630 and ABZR87_RS09635 are separated by 30 nucleotides ABZR87_RS09635 and ABZR87_RS09640 overlap by 4 nucleotides
ABZR87_RS09625: ABZR87_RS09625 - CobD/CbiB family protein, at 2,041,096 to 2,042,037
_RS09625
ABZR87_RS09630: ABZR87_RS09630 - hypothetical protein, at 2,042,074 to 2,042,388
_RS09630
ABZR87_RS09635: ABZR87_RS09635 - ribosome small subunit-dependent GTPase A, at 2,042,419 to 2,043,366
_RS09635
ABZR87_RS09640: ABZR87_RS09640 - M48 family metallopeptidase, at 2,043,363 to 2,044,622
_RS09640
Position (kb)
2042
2043
2044 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2041.612 kb on + strand, within ABZR87_RS09625 at 2041.613 kb on - strand, within ABZR87_RS09625 at 2041.635 kb on + strand, within ABZR87_RS09625 at 2041.636 kb on - strand, within ABZR87_RS09625 at 2041.636 kb on - strand, within ABZR87_RS09625 at 2041.700 kb on - strand, within ABZR87_RS09625 at 2041.700 kb on - strand, within ABZR87_RS09625 at 2041.700 kb on - strand, within ABZR87_RS09625 at 2041.762 kb on + strand, within ABZR87_RS09625 at 2041.763 kb on - strand, within ABZR87_RS09625 at 2041.763 kb on - strand, within ABZR87_RS09625 at 2041.909 kb on + strand, within ABZR87_RS09625 at 2041.923 kb on - strand, within ABZR87_RS09625 at 2041.923 kb on - strand, within ABZR87_RS09625 at 2041.923 kb on - strand, within ABZR87_RS09625 at 2042.052 kb on + strand at 2042.052 kb on + strand at 2042.053 kb on - strand at 2042.147 kb on + strand, within ABZR87_RS09630 at 2042.291 kb on + strand, within ABZR87_RS09630 at 2042.291 kb on + strand, within ABZR87_RS09630 at 2042.291 kb on + strand, within ABZR87_RS09630 at 2042.292 kb on - strand, within ABZR87_RS09630 at 2042.292 kb on - strand, within ABZR87_RS09630 at 2042.292 kb on - strand, within ABZR87_RS09630 at 2042.292 kb on - strand, within ABZR87_RS09630 at 2042.406 kb on + strand at 2042.407 kb on - strand at 2042.407 kb on - strand at 2042.421 kb on - strand at 2042.421 kb on - strand at 2042.550 kb on - strand, within ABZR87_RS09635 at 2042.550 kb on - strand, within ABZR87_RS09635 at 2042.694 kb on - strand, within ABZR87_RS09635 at 2042.694 kb on - strand, within ABZR87_RS09635 at 2043.195 kb on - strand, within ABZR87_RS09635 at 2043.195 kb on - strand, within ABZR87_RS09635 at 2043.281 kb on + strand at 2043.281 kb on + strand at 2043.282 kb on - strand at 2043.421 kb on + strand at 2043.424 kb on + strand at 2043.424 kb on + strand at 2043.425 kb on - strand at 2043.425 kb on - strand at 2043.425 kb on - strand at 2043.436 kb on + strand at 2043.436 kb on + strand at 2043.437 kb on - strand at 2043.544 kb on + strand, within ABZR87_RS09640 at 2043.545 kb on - strand, within ABZR87_RS09640 at 2043.680 kb on - strand, within ABZR87_RS09640 at 2043.680 kb on - strand, within ABZR87_RS09640 at 2043.887 kb on - strand, within ABZR87_RS09640 at 2044.018 kb on + strand, within ABZR87_RS09640 at 2044.018 kb on + strand, within ABZR87_RS09640 at 2044.043 kb on - strand, within ABZR87_RS09640 at 2044.043 kb on - strand, within ABZR87_RS09640 at 2044.090 kb on + strand, within ABZR87_RS09640 at 2044.091 kb on - strand, within ABZR87_RS09640 at 2044.091 kb on - strand, within ABZR87_RS09640 at 2044.255 kb on + strand, within ABZR87_RS09640 at 2044.256 kb on - strand, within ABZR87_RS09640 at 2044.321 kb on + strand, within ABZR87_RS09640
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 2,041,612 + ABZR87_RS09625 0.55 +0.2 2,041,613 - ABZR87_RS09625 0.55 +0.2 2,041,635 + ABZR87_RS09625 0.57 +2.0 2,041,636 - ABZR87_RS09625 0.57 -0.3 2,041,636 - ABZR87_RS09625 0.57 -0.0 2,041,700 - ABZR87_RS09625 0.64 +0.7 2,041,700 - ABZR87_RS09625 0.64 -1.0 2,041,700 - ABZR87_RS09625 0.64 +0.6 2,041,762 + ABZR87_RS09625 0.71 +0.6 2,041,763 - ABZR87_RS09625 0.71 -0.7 2,041,763 - ABZR87_RS09625 0.71 +0.9 2,041,909 + ABZR87_RS09625 0.86 +0.6 2,041,923 - ABZR87_RS09625 0.88 -0.1 2,041,923 - ABZR87_RS09625 0.88 -0.5 2,041,923 - ABZR87_RS09625 0.88 -0.3 2,042,052 + +0.3 2,042,052 + -1.3 2,042,053 - +1.3 2,042,147 + ABZR87_RS09630 0.23 +0.5 2,042,291 + ABZR87_RS09630 0.69 -1.0 2,042,291 + ABZR87_RS09630 0.69 -0.7 2,042,291 + ABZR87_RS09630 0.69 +1.4 2,042,292 - ABZR87_RS09630 0.69 -0.0 2,042,292 - ABZR87_RS09630 0.69 -1.2 2,042,292 - ABZR87_RS09630 0.69 +0.2 2,042,292 - ABZR87_RS09630 0.69 +0.5 2,042,406 + +0.9 2,042,407 - +0.5 2,042,407 - +0.6 2,042,421 - +0.5 2,042,421 - +1.3 2,042,550 - ABZR87_RS09635 0.14 -1.8 2,042,550 - ABZR87_RS09635 0.14 -1.8 2,042,694 - ABZR87_RS09635 0.29 -0.6 2,042,694 - ABZR87_RS09635 0.29 -0.3 2,043,195 - ABZR87_RS09635 0.82 -0.6 2,043,195 - ABZR87_RS09635 0.82 -0.8 2,043,281 + -1.3 2,043,281 + +1.3 2,043,282 - -0.7 2,043,421 + -1.3 2,043,424 + -1.5 2,043,424 + -1.6 2,043,425 - -0.5 2,043,425 - -0.2 2,043,425 - +0.5 2,043,436 + +2.8 2,043,436 + -1.3 2,043,437 - +0.5 2,043,544 + ABZR87_RS09640 0.14 -1.6 2,043,545 - ABZR87_RS09640 0.14 -0.7 2,043,680 - ABZR87_RS09640 0.25 -0.5 2,043,680 - ABZR87_RS09640 0.25 -0.2 2,043,887 - ABZR87_RS09640 0.42 -1.3 2,044,018 + ABZR87_RS09640 0.52 +0.0 2,044,018 + ABZR87_RS09640 0.52 +1.3 2,044,043 - ABZR87_RS09640 0.54 +2.3 2,044,043 - ABZR87_RS09640 0.54 -0.4 2,044,090 + ABZR87_RS09640 0.58 +0.1 2,044,091 - ABZR87_RS09640 0.58 +0.7 2,044,091 - ABZR87_RS09640 0.58 -0.3 2,044,255 + ABZR87_RS09640 0.71 -0.0 2,044,256 - ABZR87_RS09640 0.71 -0.8 2,044,321 + ABZR87_RS09640 0.76 -0.3
Or see this region's nucleotide sequence