Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS09610 and ABZR87_RS09615 are separated by 73 nucleotides ABZR87_RS09615 and ABZR87_RS09620 are separated by 104 nucleotides ABZR87_RS09620 and ABZR87_RS09625 are separated by 107 nucleotides
ABZR87_RS09610: ABZR87_RS09610 - tRNA (guanosine(37)-N1)-methyltransferase TrmD, at 2,038,931 to 2,039,719
_RS09610
ABZR87_RS09615: ABZR87_RS09615 - 50S ribosomal protein L19, at 2,039,793 to 2,040,179
_RS09615
ABZR87_RS09620: ABZR87_RS09620 - CoA pyrophosphatase, at 2,040,284 to 2,040,988
_RS09620
ABZR87_RS09625: ABZR87_RS09625 - CobD/CbiB family protein, at 2,041,096 to 2,042,037
_RS09625
Position (kb)
2040
2041 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2040.097 kb on - strand, within ABZR87_RS09615 at 2040.099 kb on + strand, within ABZR87_RS09615 at 2040.100 kb on - strand, within ABZR87_RS09615 at 2040.177 kb on + strand at 2040.177 kb on + strand at 2040.178 kb on - strand at 2040.250 kb on + strand at 2040.250 kb on + strand at 2040.250 kb on + strand at 2040.250 kb on + strand at 2040.250 kb on + strand at 2040.250 kb on + strand at 2040.250 kb on + strand at 2040.250 kb on + strand at 2040.250 kb on + strand at 2040.250 kb on + strand at 2040.250 kb on + strand at 2040.250 kb on + strand at 2040.250 kb on + strand at 2040.250 kb on + strand at 2040.250 kb on + strand at 2040.250 kb on + strand at 2040.251 kb on - strand at 2040.251 kb on - strand at 2040.251 kb on - strand at 2040.251 kb on - strand at 2040.251 kb on - strand at 2040.251 kb on - strand at 2040.251 kb on - strand at 2040.251 kb on - strand at 2040.251 kb on - strand at 2040.251 kb on - strand at 2040.251 kb on - strand at 2040.251 kb on - strand at 2040.251 kb on - strand at 2040.251 kb on - strand at 2040.251 kb on - strand at 2040.251 kb on - strand at 2040.544 kb on + strand, within ABZR87_RS09620 at 2040.689 kb on + strand, within ABZR87_RS09620 at 2040.690 kb on - strand, within ABZR87_RS09620 at 2040.690 kb on - strand, within ABZR87_RS09620 at 2040.690 kb on - strand, within ABZR87_RS09620 at 2040.690 kb on - strand, within ABZR87_RS09620 at 2040.690 kb on - strand, within ABZR87_RS09620 at 2040.690 kb on - strand, within ABZR87_RS09620 at 2040.690 kb on - strand, within ABZR87_RS09620 at 2040.869 kb on + strand, within ABZR87_RS09620 at 2040.869 kb on + strand, within ABZR87_RS09620 at 2040.869 kb on + strand, within ABZR87_RS09620 at 2040.870 kb on - strand, within ABZR87_RS09620 at 2040.870 kb on - strand, within ABZR87_RS09620 at 2040.870 kb on - strand, within ABZR87_RS09620 at 2040.870 kb on - strand, within ABZR87_RS09620 at 2040.969 kb on - strand at 2040.969 kb on - strand at 2040.986 kb on + strand at 2041.002 kb on - strand at 2041.040 kb on + strand at 2041.040 kb on + strand at 2041.112 kb on + strand at 2041.112 kb on + strand at 2041.113 kb on - strand at 2041.113 kb on - strand at 2041.168 kb on + strand at 2041.168 kb on + strand at 2041.294 kb on + strand, within ABZR87_RS09625 at 2041.295 kb on - strand, within ABZR87_RS09625 at 2041.351 kb on + strand, within ABZR87_RS09625 at 2041.351 kb on + strand, within ABZR87_RS09625 at 2041.352 kb on - strand, within ABZR87_RS09625 at 2041.352 kb on - strand, within ABZR87_RS09625 at 2041.352 kb on - strand, within ABZR87_RS09625 at 2041.352 kb on - strand, within ABZR87_RS09625 at 2041.384 kb on + strand, within ABZR87_RS09625 at 2041.384 kb on + strand, within ABZR87_RS09625 at 2041.384 kb on + strand, within ABZR87_RS09625 at 2041.384 kb on + strand, within ABZR87_RS09625 at 2041.385 kb on - strand, within ABZR87_RS09625 at 2041.385 kb on - strand, within ABZR87_RS09625 at 2041.385 kb on - strand, within ABZR87_RS09625 at 2041.612 kb on + strand, within ABZR87_RS09625 at 2041.613 kb on - strand, within ABZR87_RS09625 at 2041.635 kb on + strand, within ABZR87_RS09625 at 2041.636 kb on - strand, within ABZR87_RS09625 at 2041.636 kb on - strand, within ABZR87_RS09625 at 2041.700 kb on - strand, within ABZR87_RS09625 at 2041.700 kb on - strand, within ABZR87_RS09625 at 2041.700 kb on - strand, within ABZR87_RS09625 at 2041.762 kb on + strand, within ABZR87_RS09625 at 2041.763 kb on - strand, within ABZR87_RS09625 at 2041.763 kb on - strand, within ABZR87_RS09625 at 2041.909 kb on + strand, within ABZR87_RS09625 at 2041.923 kb on - strand, within ABZR87_RS09625 at 2041.923 kb on - strand, within ABZR87_RS09625 at 2041.923 kb on - strand, within ABZR87_RS09625
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 2,040,097 - ABZR87_RS09615 0.79 -1.7 2,040,099 + ABZR87_RS09615 0.79 -0.3 2,040,100 - ABZR87_RS09615 0.79 -1.0 2,040,177 + +1.0 2,040,177 + -0.2 2,040,178 - +1.0 2,040,250 + -1.2 2,040,250 + -0.2 2,040,250 + -0.1 2,040,250 + +0.1 2,040,250 + -1.6 2,040,250 + -0.6 2,040,250 + -1.5 2,040,250 + +0.1 2,040,250 + +0.1 2,040,250 + -0.4 2,040,250 + +1.4 2,040,250 + +0.6 2,040,250 + +1.4 2,040,250 + -0.2 2,040,250 + -2.0 2,040,250 + -1.7 2,040,251 - -0.2 2,040,251 - -0.9 2,040,251 - +0.8 2,040,251 - -0.0 2,040,251 - +0.4 2,040,251 - +0.9 2,040,251 - -0.3 2,040,251 - +0.8 2,040,251 - -0.1 2,040,251 - -0.4 2,040,251 - -0.1 2,040,251 - +0.2 2,040,251 - +0.6 2,040,251 - -1.2 2,040,251 - -0.0 2,040,251 - -0.7 2,040,544 + ABZR87_RS09620 0.37 +0.6 2,040,689 + ABZR87_RS09620 0.57 -0.5 2,040,690 - ABZR87_RS09620 0.58 +0.1 2,040,690 - ABZR87_RS09620 0.58 +0.1 2,040,690 - ABZR87_RS09620 0.58 +0.6 2,040,690 - ABZR87_RS09620 0.58 +0.4 2,040,690 - ABZR87_RS09620 0.58 +1.3 2,040,690 - ABZR87_RS09620 0.58 +0.1 2,040,690 - ABZR87_RS09620 0.58 +0.6 2,040,869 + ABZR87_RS09620 0.83 +0.5 2,040,869 + ABZR87_RS09620 0.83 -1.1 2,040,869 + ABZR87_RS09620 0.83 +1.1 2,040,870 - ABZR87_RS09620 0.83 -0.0 2,040,870 - ABZR87_RS09620 0.83 +0.4 2,040,870 - ABZR87_RS09620 0.83 -0.1 2,040,870 - ABZR87_RS09620 0.83 -1.4 2,040,969 - +1.0 2,040,969 - +0.4 2,040,986 + +0.6 2,041,002 - +0.7 2,041,040 + -0.7 2,041,040 + -0.9 2,041,112 + -0.7 2,041,112 + -0.1 2,041,113 - -0.7 2,041,113 - +0.3 2,041,168 + +0.7 2,041,168 + +0.6 2,041,294 + ABZR87_RS09625 0.21 -0.3 2,041,295 - ABZR87_RS09625 0.21 -2.0 2,041,351 + ABZR87_RS09625 0.27 +0.2 2,041,351 + ABZR87_RS09625 0.27 -0.1 2,041,352 - ABZR87_RS09625 0.27 -0.2 2,041,352 - ABZR87_RS09625 0.27 -0.1 2,041,352 - ABZR87_RS09625 0.27 -0.5 2,041,352 - ABZR87_RS09625 0.27 -0.3 2,041,384 + ABZR87_RS09625 0.31 +0.4 2,041,384 + ABZR87_RS09625 0.31 +0.7 2,041,384 + ABZR87_RS09625 0.31 -1.2 2,041,384 + ABZR87_RS09625 0.31 +0.0 2,041,385 - ABZR87_RS09625 0.31 +1.6 2,041,385 - ABZR87_RS09625 0.31 -0.9 2,041,385 - ABZR87_RS09625 0.31 -0.1 2,041,612 + ABZR87_RS09625 0.55 +0.2 2,041,613 - ABZR87_RS09625 0.55 +0.2 2,041,635 + ABZR87_RS09625 0.57 +2.0 2,041,636 - ABZR87_RS09625 0.57 -0.3 2,041,636 - ABZR87_RS09625 0.57 -0.0 2,041,700 - ABZR87_RS09625 0.64 +0.7 2,041,700 - ABZR87_RS09625 0.64 -1.0 2,041,700 - ABZR87_RS09625 0.64 +0.6 2,041,762 + ABZR87_RS09625 0.71 +0.6 2,041,763 - ABZR87_RS09625 0.71 -0.7 2,041,763 - ABZR87_RS09625 0.71 +0.9 2,041,909 + ABZR87_RS09625 0.86 +0.6 2,041,923 - ABZR87_RS09625 0.88 -0.1 2,041,923 - ABZR87_RS09625 0.88 -0.5 2,041,923 - ABZR87_RS09625 0.88 -0.3
Or see this region's nucleotide sequence