Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS09165 and ABZR87_RS09170 are separated by 21 nucleotides ABZR87_RS09170 and ABZR87_RS09175 are separated by 81 nucleotides ABZR87_RS09175 and ABZR87_RS09180 are separated by 12 nucleotides
ABZR87_RS09165: ABZR87_RS09165 - cytochrome c, at 1,939,199 to 1,940,482
_RS09165
ABZR87_RS09170: ABZR87_RS09170 - AsmA family protein, at 1,940,504 to 1,942,801
_RS09170
ABZR87_RS09175: ABZR87_RS09175 - tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA, at 1,942,883 to 1,943,785
_RS09175
ABZR87_RS09180: ABZR87_RS09180 - thioredoxin domain-containing protein, at 1,943,798 to 1,944,121
_RS09180
Position (kb)
1940
1941
1942
1943 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1939.530 kb on - strand, within ABZR87_RS09165 at 1939.538 kb on + strand, within ABZR87_RS09165 at 1939.539 kb on - strand, within ABZR87_RS09165 at 1939.574 kb on + strand, within ABZR87_RS09165 at 1939.574 kb on + strand, within ABZR87_RS09165 at 1939.575 kb on - strand, within ABZR87_RS09165 at 1939.575 kb on - strand, within ABZR87_RS09165 at 1939.575 kb on - strand, within ABZR87_RS09165 at 1939.575 kb on - strand, within ABZR87_RS09165 at 1939.581 kb on - strand, within ABZR87_RS09165 at 1939.581 kb on - strand, within ABZR87_RS09165 at 1939.700 kb on + strand, within ABZR87_RS09165 at 1939.700 kb on + strand, within ABZR87_RS09165 at 1939.701 kb on - strand, within ABZR87_RS09165 at 1939.745 kb on + strand, within ABZR87_RS09165 at 1939.745 kb on + strand, within ABZR87_RS09165 at 1939.746 kb on - strand, within ABZR87_RS09165 at 1939.746 kb on - strand, within ABZR87_RS09165 at 1939.783 kb on + strand, within ABZR87_RS09165 at 1939.784 kb on - strand, within ABZR87_RS09165 at 1939.784 kb on - strand, within ABZR87_RS09165 at 1939.784 kb on - strand, within ABZR87_RS09165 at 1939.784 kb on - strand, within ABZR87_RS09165 at 1939.784 kb on - strand, within ABZR87_RS09165 at 1939.838 kb on + strand, within ABZR87_RS09165 at 1939.937 kb on + strand, within ABZR87_RS09165 at 1939.937 kb on + strand, within ABZR87_RS09165 at 1939.937 kb on + strand, within ABZR87_RS09165 at 1939.937 kb on + strand, within ABZR87_RS09165 at 1939.937 kb on + strand, within ABZR87_RS09165 at 1939.938 kb on - strand, within ABZR87_RS09165 at 1940.051 kb on + strand, within ABZR87_RS09165 at 1940.052 kb on - strand, within ABZR87_RS09165 at 1940.052 kb on - strand, within ABZR87_RS09165 at 1940.180 kb on + strand, within ABZR87_RS09165 at 1940.181 kb on - strand, within ABZR87_RS09165 at 1940.181 kb on - strand, within ABZR87_RS09165 at 1940.197 kb on + strand, within ABZR87_RS09165 at 1940.197 kb on + strand, within ABZR87_RS09165 at 1940.197 kb on + strand, within ABZR87_RS09165 at 1940.197 kb on + strand, within ABZR87_RS09165 at 1940.198 kb on - strand, within ABZR87_RS09165 at 1940.198 kb on - strand, within ABZR87_RS09165 at 1940.198 kb on - strand, within ABZR87_RS09165 at 1940.237 kb on + strand, within ABZR87_RS09165 at 1940.237 kb on + strand, within ABZR87_RS09165 at 1940.237 kb on + strand, within ABZR87_RS09165 at 1940.237 kb on + strand, within ABZR87_RS09165 at 1940.237 kb on + strand, within ABZR87_RS09165 at 1940.238 kb on - strand, within ABZR87_RS09165 at 1940.238 kb on - strand, within ABZR87_RS09165 at 1940.240 kb on + strand, within ABZR87_RS09165 at 1940.241 kb on - strand, within ABZR87_RS09165 at 1940.281 kb on + strand, within ABZR87_RS09165 at 1940.281 kb on + strand, within ABZR87_RS09165 at 1940.371 kb on - strand at 1940.402 kb on + strand at 1940.402 kb on + strand at 1940.402 kb on + strand at 1940.402 kb on + strand at 1940.402 kb on + strand at 1940.402 kb on + strand at 1940.403 kb on - strand at 1940.403 kb on - strand at 1940.403 kb on - strand at 1940.403 kb on - strand at 1940.403 kb on - strand at 1940.403 kb on - strand at 1940.480 kb on + strand at 1940.481 kb on - strand at 1940.481 kb on - strand at 1940.481 kb on - strand at 1940.646 kb on + strand at 1940.646 kb on + strand at 1940.646 kb on + strand at 1940.647 kb on - strand at 1940.647 kb on - strand at 1940.747 kb on + strand, within ABZR87_RS09170 at 1940.841 kb on + strand, within ABZR87_RS09170 at 1940.841 kb on + strand, within ABZR87_RS09170 at 1940.842 kb on - strand, within ABZR87_RS09170 at 1940.842 kb on - strand, within ABZR87_RS09170 at 1940.842 kb on - strand, within ABZR87_RS09170 at 1940.842 kb on - strand, within ABZR87_RS09170 at 1940.842 kb on - strand, within ABZR87_RS09170 at 1940.842 kb on - strand, within ABZR87_RS09170 at 1940.856 kb on + strand, within ABZR87_RS09170 at 1940.856 kb on + strand, within ABZR87_RS09170 at 1940.857 kb on - strand, within ABZR87_RS09170 at 1940.857 kb on - strand, within ABZR87_RS09170 at 1940.857 kb on - strand, within ABZR87_RS09170 at 1940.857 kb on - strand, within ABZR87_RS09170 at 1940.911 kb on + strand, within ABZR87_RS09170 at 1940.911 kb on + strand, within ABZR87_RS09170 at 1941.424 kb on + strand, within ABZR87_RS09170 at 1941.425 kb on - strand, within ABZR87_RS09170 at 1941.425 kb on - strand, within ABZR87_RS09170 at 1941.711 kb on + strand, within ABZR87_RS09170 at 1941.711 kb on + strand, within ABZR87_RS09170 at 1941.711 kb on + strand, within ABZR87_RS09170 at 1941.711 kb on + strand, within ABZR87_RS09170 at 1941.712 kb on - strand, within ABZR87_RS09170 at 1941.712 kb on - strand, within ABZR87_RS09170 at 1941.712 kb on - strand, within ABZR87_RS09170 at 1941.712 kb on - strand, within ABZR87_RS09170 at 1941.887 kb on + strand, within ABZR87_RS09170 at 1942.062 kb on + strand, within ABZR87_RS09170 at 1942.062 kb on + strand, within ABZR87_RS09170 at 1942.062 kb on + strand, within ABZR87_RS09170 at 1942.062 kb on + strand, within ABZR87_RS09170 at 1942.063 kb on - strand, within ABZR87_RS09170 at 1942.063 kb on - strand, within ABZR87_RS09170 at 1942.063 kb on - strand, within ABZR87_RS09170 at 1942.063 kb on - strand, within ABZR87_RS09170 at 1942.063 kb on - strand, within ABZR87_RS09170 at 1942.063 kb on - strand, within ABZR87_RS09170 at 1942.063 kb on - strand, within ABZR87_RS09170 at 1942.093 kb on - strand, within ABZR87_RS09170 at 1942.093 kb on - strand, within ABZR87_RS09170 at 1942.218 kb on + strand, within ABZR87_RS09170 at 1942.219 kb on - strand, within ABZR87_RS09170 at 1942.219 kb on - strand, within ABZR87_RS09170 at 1942.219 kb on - strand, within ABZR87_RS09170 at 1942.219 kb on - strand, within ABZR87_RS09170 at 1942.319 kb on + strand, within ABZR87_RS09170 at 1942.320 kb on - strand, within ABZR87_RS09170 at 1942.587 kb on + strand at 1942.587 kb on + strand at 1942.587 kb on + strand at 1942.587 kb on + strand at 1942.587 kb on + strand at 1942.588 kb on - strand at 1942.588 kb on - strand at 1942.588 kb on - strand at 1942.588 kb on - strand at 1942.692 kb on + strand at 1942.692 kb on + strand at 1942.795 kb on + strand at 1942.795 kb on + strand at 1942.795 kb on + strand at 1942.795 kb on + strand at 1942.795 kb on + strand at 1942.795 kb on + strand at 1942.795 kb on + strand at 1942.795 kb on + strand at 1942.796 kb on - strand at 1942.796 kb on - strand at 1942.796 kb on - strand at 1942.796 kb on - strand at 1942.796 kb on - strand at 1942.796 kb on - strand at 1942.817 kb on - strand at 1942.817 kb on - strand at 1942.819 kb on - strand at 1942.848 kb on + strand at 1942.848 kb on + strand at 1942.848 kb on + strand at 1942.848 kb on + strand at 1942.848 kb on + strand at 1942.848 kb on + strand at 1942.848 kb on + strand at 1942.848 kb on + strand at 1942.848 kb on + strand at 1942.848 kb on + strand at 1942.848 kb on + strand at 1942.849 kb on - strand at 1942.849 kb on - strand at 1942.849 kb on - strand at 1942.849 kb on - strand at 1942.849 kb on - strand at 1942.849 kb on - strand at 1942.849 kb on - strand at 1942.849 kb on - strand at 1942.849 kb on - strand at 1942.850 kb on + strand at 1942.850 kb on + strand at 1942.850 kb on + strand at 1942.850 kb on + strand at 1942.850 kb on + strand at 1942.850 kb on + strand at 1942.850 kb on + strand at 1942.850 kb on + strand at 1942.850 kb on + strand at 1942.851 kb on - strand at 1942.851 kb on - strand at 1942.851 kb on - strand at 1942.851 kb on - strand at 1942.851 kb on - strand at 1942.851 kb on - strand at 1942.851 kb on - strand at 1942.852 kb on + strand at 1942.852 kb on + strand at 1942.852 kb on + strand at 1942.852 kb on + strand at 1942.852 kb on + strand at 1942.853 kb on - strand at 1942.853 kb on - strand at 1942.853 kb on - strand at 1942.853 kb on - strand at 1942.853 kb on - strand at 1942.893 kb on + strand at 1942.893 kb on + strand at 1942.893 kb on + strand at 1942.893 kb on + strand at 1942.894 kb on - strand at 1942.937 kb on + strand at 1942.937 kb on + strand at 1942.937 kb on + strand at 1942.937 kb on + strand at 1942.938 kb on - strand at 1942.970 kb on + strand at 1942.971 kb on - strand at 1942.971 kb on - strand at 1943.153 kb on + strand, within ABZR87_RS09175 at 1943.153 kb on + strand, within ABZR87_RS09175 at 1943.282 kb on + strand, within ABZR87_RS09175 at 1943.283 kb on - strand, within ABZR87_RS09175 at 1943.718 kb on + strand at 1943.718 kb on + strand at 1943.718 kb on + strand at 1943.718 kb on + strand at 1943.719 kb on - strand at 1943.719 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 1,939,530 - ABZR87_RS09165 0.26 +1.3 1,939,538 + ABZR87_RS09165 0.26 +0.1 1,939,539 - ABZR87_RS09165 0.26 +1.0 1,939,574 + ABZR87_RS09165 0.29 +0.4 1,939,574 + ABZR87_RS09165 0.29 -1.4 1,939,575 - ABZR87_RS09165 0.29 -1.1 1,939,575 - ABZR87_RS09165 0.29 -0.6 1,939,575 - ABZR87_RS09165 0.29 +1.0 1,939,575 - ABZR87_RS09165 0.29 +1.4 1,939,581 - ABZR87_RS09165 0.30 -0.2 1,939,581 - ABZR87_RS09165 0.30 -0.3 1,939,700 + ABZR87_RS09165 0.39 +0.3 1,939,700 + ABZR87_RS09165 0.39 -0.5 1,939,701 - ABZR87_RS09165 0.39 -2.0 1,939,745 + ABZR87_RS09165 0.43 +0.3 1,939,745 + ABZR87_RS09165 0.43 -0.1 1,939,746 - ABZR87_RS09165 0.43 +0.6 1,939,746 - ABZR87_RS09165 0.43 +0.2 1,939,783 + ABZR87_RS09165 0.45 -0.6 1,939,784 - ABZR87_RS09165 0.46 +2.8 1,939,784 - ABZR87_RS09165 0.46 +0.2 1,939,784 - ABZR87_RS09165 0.46 -1.3 1,939,784 - ABZR87_RS09165 0.46 -0.5 1,939,784 - ABZR87_RS09165 0.46 +1.1 1,939,838 + ABZR87_RS09165 0.50 -1.0 1,939,937 + ABZR87_RS09165 0.57 -2.0 1,939,937 + ABZR87_RS09165 0.57 -0.6 1,939,937 + ABZR87_RS09165 0.57 -1.2 1,939,937 + ABZR87_RS09165 0.57 -1.8 1,939,937 + ABZR87_RS09165 0.57 +0.6 1,939,938 - ABZR87_RS09165 0.58 +0.7 1,940,051 + ABZR87_RS09165 0.66 -0.7 1,940,052 - ABZR87_RS09165 0.66 -0.6 1,940,052 - ABZR87_RS09165 0.66 -0.7 1,940,180 + ABZR87_RS09165 0.76 -0.1 1,940,181 - ABZR87_RS09165 0.76 +0.1 1,940,181 - ABZR87_RS09165 0.76 +0.8 1,940,197 + ABZR87_RS09165 0.78 +0.1 1,940,197 + ABZR87_RS09165 0.78 -1.4 1,940,197 + ABZR87_RS09165 0.78 -0.5 1,940,197 + ABZR87_RS09165 0.78 +0.8 1,940,198 - ABZR87_RS09165 0.78 -0.0 1,940,198 - ABZR87_RS09165 0.78 +0.2 1,940,198 - ABZR87_RS09165 0.78 +0.8 1,940,237 + ABZR87_RS09165 0.81 +0.3 1,940,237 + ABZR87_RS09165 0.81 +0.9 1,940,237 + ABZR87_RS09165 0.81 -0.3 1,940,237 + ABZR87_RS09165 0.81 -0.3 1,940,237 + ABZR87_RS09165 0.81 -1.9 1,940,238 - ABZR87_RS09165 0.81 +1.3 1,940,238 - ABZR87_RS09165 0.81 -0.9 1,940,240 + ABZR87_RS09165 0.81 +0.8 1,940,241 - ABZR87_RS09165 0.81 +0.4 1,940,281 + ABZR87_RS09165 0.84 -0.1 1,940,281 + ABZR87_RS09165 0.84 +0.1 1,940,371 - +2.3 1,940,402 + +0.4 1,940,402 + +0.7 1,940,402 + +0.3 1,940,402 + +0.3 1,940,402 + -0.8 1,940,402 + +0.8 1,940,403 - -0.4 1,940,403 - +0.2 1,940,403 - +0.5 1,940,403 - -0.0 1,940,403 - -1.5 1,940,403 - +0.7 1,940,480 + -0.2 1,940,481 - -1.1 1,940,481 - -0.7 1,940,481 - +1.7 1,940,646 + -0.4 1,940,646 + -1.5 1,940,646 + +0.1 1,940,647 - +0.4 1,940,647 - -0.3 1,940,747 + ABZR87_RS09170 0.11 +0.3 1,940,841 + ABZR87_RS09170 0.15 -1.6 1,940,841 + ABZR87_RS09170 0.15 -1.2 1,940,842 - ABZR87_RS09170 0.15 +0.5 1,940,842 - ABZR87_RS09170 0.15 -0.1 1,940,842 - ABZR87_RS09170 0.15 +0.3 1,940,842 - ABZR87_RS09170 0.15 -0.2 1,940,842 - ABZR87_RS09170 0.15 -1.2 1,940,842 - ABZR87_RS09170 0.15 +2.2 1,940,856 + ABZR87_RS09170 0.15 +0.3 1,940,856 + ABZR87_RS09170 0.15 +1.3 1,940,857 - ABZR87_RS09170 0.15 -0.5 1,940,857 - ABZR87_RS09170 0.15 -0.6 1,940,857 - ABZR87_RS09170 0.15 +0.3 1,940,857 - ABZR87_RS09170 0.15 +1.8 1,940,911 + ABZR87_RS09170 0.18 -0.6 1,940,911 + ABZR87_RS09170 0.18 -1.1 1,941,424 + ABZR87_RS09170 0.40 -0.7 1,941,425 - ABZR87_RS09170 0.40 -0.9 1,941,425 - ABZR87_RS09170 0.40 +1.3 1,941,711 + ABZR87_RS09170 0.53 -0.4 1,941,711 + ABZR87_RS09170 0.53 -1.9 1,941,711 + ABZR87_RS09170 0.53 -1.8 1,941,711 + ABZR87_RS09170 0.53 +1.1 1,941,712 - ABZR87_RS09170 0.53 -1.3 1,941,712 - ABZR87_RS09170 0.53 -1.0 1,941,712 - ABZR87_RS09170 0.53 -0.5 1,941,712 - ABZR87_RS09170 0.53 +0.2 1,941,887 + ABZR87_RS09170 0.60 -0.6 1,942,062 + ABZR87_RS09170 0.68 -0.7 1,942,062 + ABZR87_RS09170 0.68 -0.2 1,942,062 + ABZR87_RS09170 0.68 -0.3 1,942,062 + ABZR87_RS09170 0.68 -0.3 1,942,063 - ABZR87_RS09170 0.68 -0.9 1,942,063 - ABZR87_RS09170 0.68 -0.8 1,942,063 - ABZR87_RS09170 0.68 -0.1 1,942,063 - ABZR87_RS09170 0.68 +0.4 1,942,063 - ABZR87_RS09170 0.68 +0.4 1,942,063 - ABZR87_RS09170 0.68 -0.4 1,942,063 - ABZR87_RS09170 0.68 +0.1 1,942,093 - ABZR87_RS09170 0.69 -0.7 1,942,093 - ABZR87_RS09170 0.69 +1.1 1,942,218 + ABZR87_RS09170 0.75 +0.8 1,942,219 - ABZR87_RS09170 0.75 -0.3 1,942,219 - ABZR87_RS09170 0.75 -1.6 1,942,219 - ABZR87_RS09170 0.75 -0.2 1,942,219 - ABZR87_RS09170 0.75 -0.7 1,942,319 + ABZR87_RS09170 0.79 -0.5 1,942,320 - ABZR87_RS09170 0.79 -0.5 1,942,587 + -1.5 1,942,587 + -1.5 1,942,587 + +0.1 1,942,587 + -0.1 1,942,587 + -0.0 1,942,588 - +1.1 1,942,588 - -0.2 1,942,588 - +0.2 1,942,588 - +0.3 1,942,692 + -0.5 1,942,692 + -0.4 1,942,795 + -0.2 1,942,795 + +1.3 1,942,795 + +0.0 1,942,795 + -0.5 1,942,795 + -0.3 1,942,795 + -1.4 1,942,795 + -1.0 1,942,795 + +2.6 1,942,796 - -0.5 1,942,796 - -0.4 1,942,796 - +0.8 1,942,796 - +1.4 1,942,796 - -1.3 1,942,796 - -0.1 1,942,817 - -0.1 1,942,817 - +0.5 1,942,819 - -0.1 1,942,848 + +1.5 1,942,848 + +0.9 1,942,848 + +0.1 1,942,848 + +0.0 1,942,848 + +0.8 1,942,848 + +1.0 1,942,848 + -1.5 1,942,848 + -1.6 1,942,848 + +0.2 1,942,848 + +0.6 1,942,848 + +0.8 1,942,849 - -1.0 1,942,849 - -0.4 1,942,849 - -1.2 1,942,849 - -0.6 1,942,849 - -2.3 1,942,849 - -0.1 1,942,849 - -0.1 1,942,849 - +0.9 1,942,849 - -1.3 1,942,850 + +1.0 1,942,850 + +0.1 1,942,850 + +0.5 1,942,850 + -0.6 1,942,850 + -1.2 1,942,850 + +0.3 1,942,850 + +0.9 1,942,850 + -0.0 1,942,850 + -0.2 1,942,851 - -0.3 1,942,851 - -0.9 1,942,851 - +0.2 1,942,851 - +0.3 1,942,851 - -0.5 1,942,851 - -2.2 1,942,851 - -1.0 1,942,852 + -0.1 1,942,852 + +0.8 1,942,852 + -0.6 1,942,852 + -0.4 1,942,852 + -1.5 1,942,853 - -0.8 1,942,853 - +0.2 1,942,853 - +0.3 1,942,853 - +0.6 1,942,853 - +0.3 1,942,893 + -0.1 1,942,893 + -0.3 1,942,893 + +0.6 1,942,893 + +0.1 1,942,894 - -0.9 1,942,937 + -0.6 1,942,937 + -0.5 1,942,937 + -0.8 1,942,937 + -0.2 1,942,938 - -0.5 1,942,970 + +0.1 1,942,971 - -0.5 1,942,971 - -0.7 1,943,153 + ABZR87_RS09175 0.30 +0.8 1,943,153 + ABZR87_RS09175 0.30 +0.2 1,943,282 + ABZR87_RS09175 0.44 -0.2 1,943,283 - ABZR87_RS09175 0.44 -0.5 1,943,718 + -1.0 1,943,718 + +0.1 1,943,718 + -0.6 1,943,718 + +0.8 1,943,719 - +0.2 1,943,719 - -0.0
Or see this region's nucleotide sequence