Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS06750 and ABZR87_RS06755 are separated by 177 nucleotides ABZR87_RS06755 and ABZR87_RS06760 are separated by 18 nucleotides ABZR87_RS06760 and ABZR87_RS06765 are separated by 172 nucleotides ABZR87_RS06765 and ABZR87_RS06770 are separated by 108 nucleotides
ABZR87_RS06750: ABZR87_RS06750 - 2,3-diphosphoglycerate-dependent phosphoglycerate mutase, at 1,434,581 to 1,435,327
_RS06750
ABZR87_RS06755: ABZR87_RS06755 - rhodanese-like domain-containing protein, at 1,435,505 to 1,435,927
_RS06755
ABZR87_RS06760: ABZR87_RS06760 - glutaredoxin 3, at 1,435,946 to 1,436,203
_RS06760
ABZR87_RS06765: ABZR87_RS06765 - protein-export chaperone SecB, at 1,436,376 to 1,436,894
_RS06765
ABZR87_RS06770: ABZR87_RS06770 - NAD(P)H-dependent glycerol-3-phosphate dehydrogenase, at 1,437,003 to 1,438,019
_RS06770
Position (kb)
1435
1436
1437 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1435.384 kb on + strand at 1435.385 kb on - strand at 1435.385 kb on - strand at 1435.385 kb on - strand at 1435.385 kb on - strand at 1435.385 kb on - strand at 1435.385 kb on - strand at 1435.414 kb on - strand at 1435.414 kb on - strand at 1435.414 kb on - strand at 1435.414 kb on - strand at 1435.418 kb on + strand at 1435.418 kb on + strand at 1435.418 kb on + strand at 1435.418 kb on + strand at 1435.419 kb on - strand at 1435.419 kb on - strand at 1435.419 kb on - strand at 1435.421 kb on - strand at 1435.470 kb on + strand at 1435.470 kb on + strand at 1435.470 kb on + strand at 1435.471 kb on - strand at 1435.471 kb on - strand at 1435.471 kb on - strand at 1435.524 kb on - strand at 1435.524 kb on - strand at 1435.698 kb on - strand, within ABZR87_RS06755 at 1435.800 kb on + strand, within ABZR87_RS06755 at 1435.800 kb on + strand, within ABZR87_RS06755 at 1435.801 kb on - strand, within ABZR87_RS06755 at 1435.801 kb on - strand, within ABZR87_RS06755 at 1435.801 kb on - strand, within ABZR87_RS06755 at 1435.801 kb on - strand, within ABZR87_RS06755 at 1435.801 kb on - strand, within ABZR87_RS06755 at 1435.801 kb on - strand, within ABZR87_RS06755 at 1435.857 kb on - strand, within ABZR87_RS06755 at 1435.857 kb on - strand, within ABZR87_RS06755 at 1435.868 kb on + strand, within ABZR87_RS06755 at 1435.868 kb on + strand, within ABZR87_RS06755 at 1435.868 kb on + strand, within ABZR87_RS06755 at 1435.868 kb on + strand, within ABZR87_RS06755 at 1435.868 kb on + strand, within ABZR87_RS06755 at 1435.869 kb on - strand, within ABZR87_RS06755 at 1435.869 kb on - strand, within ABZR87_RS06755 at 1435.869 kb on - strand, within ABZR87_RS06755 at 1435.925 kb on + strand at 1435.925 kb on + strand at 1435.925 kb on + strand at 1435.925 kb on + strand at 1435.926 kb on - strand at 1435.926 kb on - strand at 1435.926 kb on - strand at 1435.926 kb on - strand at 1435.931 kb on + strand at 1435.931 kb on + strand at 1435.932 kb on - strand at 1435.932 kb on - strand at 1435.932 kb on - strand at 1435.932 kb on - strand at 1435.932 kb on - strand at 1435.932 kb on - strand at 1435.945 kb on + strand at 1435.964 kb on + strand at 1435.964 kb on + strand at 1435.964 kb on + strand at 1435.964 kb on + strand at 1435.964 kb on + strand at 1435.964 kb on + strand at 1435.964 kb on + strand at 1435.965 kb on - strand at 1435.965 kb on - strand at 1435.965 kb on - strand at 1435.965 kb on - strand at 1435.965 kb on - strand at 1435.965 kb on - strand at 1435.965 kb on - strand at 1435.985 kb on + strand, within ABZR87_RS06760 at 1435.986 kb on - strand, within ABZR87_RS06760 at 1436.117 kb on + strand, within ABZR87_RS06760 at 1436.117 kb on + strand, within ABZR87_RS06760 at 1436.117 kb on + strand, within ABZR87_RS06760 at 1436.117 kb on + strand, within ABZR87_RS06760 at 1436.117 kb on + strand, within ABZR87_RS06760 at 1436.117 kb on + strand, within ABZR87_RS06760 at 1436.118 kb on - strand, within ABZR87_RS06760 at 1436.119 kb on + strand, within ABZR87_RS06760 at 1436.120 kb on - strand, within ABZR87_RS06760 at 1436.120 kb on - strand, within ABZR87_RS06760 at 1436.266 kb on + strand at 1436.266 kb on + strand at 1436.308 kb on + strand at 1436.308 kb on + strand at 1436.308 kb on + strand at 1436.308 kb on + strand at 1436.309 kb on - strand at 1436.309 kb on - strand at 1436.309 kb on - strand at 1436.309 kb on - strand at 1436.309 kb on - strand at 1436.309 kb on - strand at 1436.309 kb on - strand at 1436.309 kb on - strand at 1436.309 kb on - strand at 1436.313 kb on + strand at 1436.313 kb on + strand at 1436.313 kb on + strand at 1436.313 kb on + strand at 1436.313 kb on + strand at 1436.892 kb on + strand at 1436.892 kb on + strand at 1436.892 kb on + strand at 1436.893 kb on - strand at 1436.893 kb on - strand at 1436.893 kb on - strand at 1436.893 kb on - strand at 1436.893 kb on - strand at 1436.893 kb on - strand at 1436.893 kb on - strand at 1436.897 kb on - strand at 1436.897 kb on - strand at 1436.897 kb on - strand at 1436.897 kb on - strand at 1436.897 kb on - strand at 1436.897 kb on - strand at 1437.141 kb on + strand, within ABZR87_RS06770 at 1437.141 kb on + strand, within ABZR87_RS06770 at 1437.142 kb on - strand, within ABZR87_RS06770
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 1,435,384 + -0.2 1,435,385 - -0.4 1,435,385 - +0.1 1,435,385 - +0.4 1,435,385 - -0.2 1,435,385 - -1.3 1,435,385 - -0.2 1,435,414 - +0.7 1,435,414 - -0.6 1,435,414 - +0.5 1,435,414 - -1.2 1,435,418 + +0.1 1,435,418 + +0.2 1,435,418 + -1.3 1,435,418 + -0.2 1,435,419 - -0.4 1,435,419 - +0.2 1,435,419 - -0.0 1,435,421 - -0.4 1,435,470 + -0.4 1,435,470 + +0.2 1,435,470 + -0.1 1,435,471 - +1.6 1,435,471 - +0.0 1,435,471 - -0.4 1,435,524 - -0.6 1,435,524 - -1.1 1,435,698 - ABZR87_RS06755 0.46 +0.5 1,435,800 + ABZR87_RS06755 0.70 +0.2 1,435,800 + ABZR87_RS06755 0.70 +0.5 1,435,801 - ABZR87_RS06755 0.70 -1.3 1,435,801 - ABZR87_RS06755 0.70 +1.3 1,435,801 - ABZR87_RS06755 0.70 +0.0 1,435,801 - ABZR87_RS06755 0.70 -0.2 1,435,801 - ABZR87_RS06755 0.70 +0.0 1,435,801 - ABZR87_RS06755 0.70 +0.3 1,435,857 - ABZR87_RS06755 0.83 -0.0 1,435,857 - ABZR87_RS06755 0.83 +0.7 1,435,868 + ABZR87_RS06755 0.86 +0.8 1,435,868 + ABZR87_RS06755 0.86 -0.7 1,435,868 + ABZR87_RS06755 0.86 +0.2 1,435,868 + ABZR87_RS06755 0.86 +1.1 1,435,868 + ABZR87_RS06755 0.86 +0.8 1,435,869 - ABZR87_RS06755 0.86 -0.2 1,435,869 - ABZR87_RS06755 0.86 +0.8 1,435,869 - ABZR87_RS06755 0.86 -0.4 1,435,925 + +0.1 1,435,925 + -0.2 1,435,925 + -0.6 1,435,925 + +0.2 1,435,926 - +0.5 1,435,926 - -1.5 1,435,926 - +0.3 1,435,926 - -0.8 1,435,931 + +0.3 1,435,931 + -0.4 1,435,932 - +0.4 1,435,932 - -1.2 1,435,932 - +0.3 1,435,932 - +0.3 1,435,932 - -0.1 1,435,932 - -0.2 1,435,945 + +0.8 1,435,964 + -0.7 1,435,964 + +0.1 1,435,964 + -2.4 1,435,964 + +0.9 1,435,964 + -1.0 1,435,964 + -1.2 1,435,964 + -1.2 1,435,965 - -0.7 1,435,965 - -0.4 1,435,965 - -0.9 1,435,965 - +0.5 1,435,965 - -1.1 1,435,965 - -0.3 1,435,965 - -0.7 1,435,985 + ABZR87_RS06760 0.15 -0.8 1,435,986 - ABZR87_RS06760 0.16 -0.5 1,436,117 + ABZR87_RS06760 0.66 +0.1 1,436,117 + ABZR87_RS06760 0.66 -0.9 1,436,117 + ABZR87_RS06760 0.66 -2.9 1,436,117 + ABZR87_RS06760 0.66 +0.2 1,436,117 + ABZR87_RS06760 0.66 -1.0 1,436,117 + ABZR87_RS06760 0.66 -0.6 1,436,118 - ABZR87_RS06760 0.67 -0.4 1,436,119 + ABZR87_RS06760 0.67 -0.7 1,436,120 - ABZR87_RS06760 0.67 -0.7 1,436,120 - ABZR87_RS06760 0.67 -0.9 1,436,266 + -0.1 1,436,266 + -1.2 1,436,308 + -0.3 1,436,308 + -0.2 1,436,308 + +0.3 1,436,308 + -1.2 1,436,309 - -0.1 1,436,309 - +1.6 1,436,309 - -0.2 1,436,309 - +0.1 1,436,309 - -0.4 1,436,309 - -0.9 1,436,309 - -0.3 1,436,309 - +0.9 1,436,309 - -0.2 1,436,313 + +0.3 1,436,313 + -0.1 1,436,313 + +0.5 1,436,313 + -0.0 1,436,313 + -0.0 1,436,892 + -0.3 1,436,892 + +0.3 1,436,892 + -1.2 1,436,893 - +0.4 1,436,893 - -1.0 1,436,893 - -0.0 1,436,893 - -1.4 1,436,893 - -1.0 1,436,893 - -1.7 1,436,893 - +0.2 1,436,897 - +1.1 1,436,897 - -1.0 1,436,897 - -1.7 1,436,897 - -1.7 1,436,897 - -1.1 1,436,897 - -1.3 1,437,141 + ABZR87_RS06770 0.14 -0.7 1,437,141 + ABZR87_RS06770 0.14 -0.5 1,437,142 - ABZR87_RS06770 0.14 -0.5
Or see this region's nucleotide sequence