Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS05825 and ABZR87_RS05830 are separated by 165 nucleotides ABZR87_RS05830 and ABZR87_RS05835 are separated by 322 nucleotides
ABZR87_RS05825: ABZR87_RS05825 - low-specificity L-threonine aldolase, at 1,241,545 to 1,242,609
_RS05825
ABZR87_RS05830: ABZR87_RS05830 - porin, at 1,242,775 to 1,243,899
_RS05830
ABZR87_RS05835: ABZR87_RS05835 - 4-hydroxythreonine-4-phosphate dehydrogenase, at 1,244,222 to 1,244,881
_RS05835
Position (kb)
1242
1243
1244 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1241.778 kb on - strand, within ABZR87_RS05825 at 1241.793 kb on + strand, within ABZR87_RS05825 at 1241.793 kb on + strand, within ABZR87_RS05825 at 1241.794 kb on - strand, within ABZR87_RS05825 at 1241.972 kb on + strand, within ABZR87_RS05825 at 1241.972 kb on + strand, within ABZR87_RS05825 at 1241.972 kb on + strand, within ABZR87_RS05825 at 1241.973 kb on - strand, within ABZR87_RS05825 at 1241.973 kb on - strand, within ABZR87_RS05825 at 1241.986 kb on + strand, within ABZR87_RS05825 at 1241.986 kb on + strand, within ABZR87_RS05825 at 1241.986 kb on + strand, within ABZR87_RS05825 at 1241.986 kb on + strand, within ABZR87_RS05825 at 1241.986 kb on + strand, within ABZR87_RS05825 at 1241.986 kb on + strand, within ABZR87_RS05825 at 1241.986 kb on + strand, within ABZR87_RS05825 at 1241.987 kb on - strand, within ABZR87_RS05825 at 1241.987 kb on - strand, within ABZR87_RS05825 at 1242.417 kb on + strand, within ABZR87_RS05825 at 1242.418 kb on - strand, within ABZR87_RS05825 at 1242.505 kb on + strand at 1242.505 kb on + strand at 1242.505 kb on + strand at 1242.505 kb on + strand at 1242.505 kb on + strand at 1242.506 kb on - strand at 1242.506 kb on - strand at 1242.585 kb on + strand at 1242.639 kb on + strand at 1242.703 kb on - strand at 1242.703 kb on - strand at 1242.802 kb on + strand at 1242.802 kb on + strand at 1242.802 kb on + strand at 1242.802 kb on + strand at 1242.802 kb on + strand at 1242.803 kb on - strand at 1242.803 kb on - strand at 1242.803 kb on - strand at 1242.899 kb on + strand, within ABZR87_RS05830 at 1242.899 kb on + strand, within ABZR87_RS05830 at 1242.899 kb on + strand, within ABZR87_RS05830 at 1242.899 kb on + strand, within ABZR87_RS05830 at 1242.899 kb on + strand, within ABZR87_RS05830 at 1242.899 kb on + strand, within ABZR87_RS05830 at 1242.899 kb on + strand, within ABZR87_RS05830 at 1242.899 kb on + strand, within ABZR87_RS05830 at 1242.900 kb on - strand, within ABZR87_RS05830 at 1242.900 kb on - strand, within ABZR87_RS05830 at 1242.900 kb on - strand, within ABZR87_RS05830 at 1242.900 kb on - strand, within ABZR87_RS05830 at 1242.900 kb on - strand, within ABZR87_RS05830 at 1242.926 kb on + strand, within ABZR87_RS05830 at 1242.926 kb on + strand, within ABZR87_RS05830 at 1242.926 kb on + strand, within ABZR87_RS05830 at 1242.926 kb on + strand, within ABZR87_RS05830 at 1242.926 kb on + strand, within ABZR87_RS05830 at 1242.926 kb on + strand, within ABZR87_RS05830 at 1242.926 kb on + strand, within ABZR87_RS05830 at 1242.927 kb on - strand, within ABZR87_RS05830 at 1242.927 kb on - strand, within ABZR87_RS05830 at 1242.927 kb on - strand, within ABZR87_RS05830 at 1242.927 kb on - strand, within ABZR87_RS05830 at 1242.927 kb on - strand, within ABZR87_RS05830 at 1243.001 kb on + strand, within ABZR87_RS05830 at 1243.001 kb on + strand, within ABZR87_RS05830 at 1243.002 kb on - strand, within ABZR87_RS05830 at 1243.002 kb on - strand, within ABZR87_RS05830 at 1243.002 kb on - strand, within ABZR87_RS05830 at 1243.002 kb on - strand, within ABZR87_RS05830 at 1243.002 kb on - strand, within ABZR87_RS05830 at 1243.038 kb on - strand, within ABZR87_RS05830 at 1243.038 kb on - strand, within ABZR87_RS05830 at 1243.196 kb on + strand, within ABZR87_RS05830 at 1243.196 kb on + strand, within ABZR87_RS05830 at 1243.196 kb on + strand, within ABZR87_RS05830 at 1243.196 kb on + strand, within ABZR87_RS05830 at 1243.197 kb on - strand, within ABZR87_RS05830 at 1243.197 kb on - strand, within ABZR87_RS05830 at 1243.199 kb on + strand, within ABZR87_RS05830 at 1243.200 kb on - strand, within ABZR87_RS05830 at 1243.200 kb on - strand, within ABZR87_RS05830 at 1243.200 kb on - strand, within ABZR87_RS05830 at 1243.228 kb on - strand, within ABZR87_RS05830 at 1243.228 kb on - strand, within ABZR87_RS05830 at 1243.229 kb on + strand, within ABZR87_RS05830 at 1243.229 kb on + strand, within ABZR87_RS05830 at 1243.230 kb on - strand, within ABZR87_RS05830 at 1243.322 kb on + strand, within ABZR87_RS05830 at 1243.322 kb on + strand, within ABZR87_RS05830 at 1243.322 kb on + strand, within ABZR87_RS05830 at 1243.323 kb on - strand, within ABZR87_RS05830 at 1243.323 kb on - strand, within ABZR87_RS05830 at 1243.385 kb on + strand, within ABZR87_RS05830 at 1243.385 kb on + strand, within ABZR87_RS05830 at 1243.385 kb on + strand, within ABZR87_RS05830 at 1243.386 kb on - strand, within ABZR87_RS05830 at 1243.386 kb on - strand, within ABZR87_RS05830 at 1243.424 kb on + strand, within ABZR87_RS05830 at 1243.424 kb on + strand, within ABZR87_RS05830 at 1243.424 kb on + strand, within ABZR87_RS05830 at 1243.443 kb on - strand, within ABZR87_RS05830 at 1243.473 kb on + strand, within ABZR87_RS05830 at 1243.473 kb on + strand, within ABZR87_RS05830 at 1243.473 kb on + strand, within ABZR87_RS05830 at 1243.473 kb on + strand, within ABZR87_RS05830 at 1243.473 kb on + strand, within ABZR87_RS05830 at 1243.473 kb on + strand, within ABZR87_RS05830 at 1243.473 kb on + strand, within ABZR87_RS05830 at 1243.474 kb on - strand, within ABZR87_RS05830 at 1243.474 kb on - strand, within ABZR87_RS05830 at 1243.474 kb on - strand, within ABZR87_RS05830 at 1243.474 kb on - strand, within ABZR87_RS05830 at 1243.484 kb on + strand, within ABZR87_RS05830 at 1243.484 kb on + strand, within ABZR87_RS05830 at 1243.485 kb on - strand, within ABZR87_RS05830 at 1243.485 kb on - strand, within ABZR87_RS05830 at 1243.526 kb on + strand, within ABZR87_RS05830 at 1243.527 kb on - strand, within ABZR87_RS05830 at 1243.635 kb on + strand, within ABZR87_RS05830 at 1243.774 kb on - strand, within ABZR87_RS05830 at 1243.791 kb on - strand at 1243.911 kb on + strand at 1243.911 kb on + strand at 1243.912 kb on - strand at 1243.914 kb on + strand at 1243.914 kb on + strand at 1243.915 kb on - strand at 1243.915 kb on - strand at 1243.915 kb on - strand at 1243.915 kb on - strand at 1243.925 kb on + strand at 1243.926 kb on - strand at 1243.926 kb on - strand at 1244.254 kb on + strand at 1244.254 kb on + strand at 1244.255 kb on - strand at 1244.255 kb on - strand at 1244.371 kb on + strand, within ABZR87_RS05835 at 1244.372 kb on - strand, within ABZR87_RS05835 at 1244.372 kb on - strand, within ABZR87_RS05835 at 1244.481 kb on - strand, within ABZR87_RS05835 at 1244.583 kb on + strand, within ABZR87_RS05835 at 1244.647 kb on - strand, within ABZR87_RS05835 at 1244.647 kb on - strand, within ABZR87_RS05835 at 1244.805 kb on + strand, within ABZR87_RS05835 at 1244.805 kb on + strand, within ABZR87_RS05835 at 1244.805 kb on + strand, within ABZR87_RS05835 at 1244.805 kb on + strand, within ABZR87_RS05835 at 1244.805 kb on + strand, within ABZR87_RS05835 at 1244.805 kb on + strand, within ABZR87_RS05835 at 1244.805 kb on + strand, within ABZR87_RS05835 at 1244.805 kb on + strand, within ABZR87_RS05835 at 1244.805 kb on + strand, within ABZR87_RS05835 at 1244.805 kb on + strand, within ABZR87_RS05835 at 1244.805 kb on + strand, within ABZR87_RS05835 at 1244.805 kb on + strand, within ABZR87_RS05835 at 1244.805 kb on + strand, within ABZR87_RS05835 at 1244.805 kb on + strand, within ABZR87_RS05835 at 1244.806 kb on - strand, within ABZR87_RS05835 at 1244.806 kb on - strand, within ABZR87_RS05835 at 1244.806 kb on - strand, within ABZR87_RS05835 at 1244.806 kb on - strand, within ABZR87_RS05835 at 1244.810 kb on + strand, within ABZR87_RS05835 at 1244.810 kb on + strand, within ABZR87_RS05835 at 1244.811 kb on - strand, within ABZR87_RS05835 at 1244.835 kb on + strand at 1244.835 kb on + strand at 1244.836 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 1,241,778 - ABZR87_RS05825 0.22 -0.6 1,241,793 + ABZR87_RS05825 0.23 +2.8 1,241,793 + ABZR87_RS05825 0.23 +1.2 1,241,794 - ABZR87_RS05825 0.23 +0.2 1,241,972 + ABZR87_RS05825 0.40 -0.8 1,241,972 + ABZR87_RS05825 0.40 -0.2 1,241,972 + ABZR87_RS05825 0.40 -0.1 1,241,973 - ABZR87_RS05825 0.40 -0.7 1,241,973 - ABZR87_RS05825 0.40 -1.6 1,241,986 + ABZR87_RS05825 0.41 -0.3 1,241,986 + ABZR87_RS05825 0.41 -0.5 1,241,986 + ABZR87_RS05825 0.41 +0.3 1,241,986 + ABZR87_RS05825 0.41 -0.0 1,241,986 + ABZR87_RS05825 0.41 +0.3 1,241,986 + ABZR87_RS05825 0.41 +0.3 1,241,986 + ABZR87_RS05825 0.41 -1.5 1,241,987 - ABZR87_RS05825 0.42 -0.4 1,241,987 - ABZR87_RS05825 0.42 +0.2 1,242,417 + ABZR87_RS05825 0.82 -0.3 1,242,418 - ABZR87_RS05825 0.82 -0.5 1,242,505 + -0.5 1,242,505 + -0.2 1,242,505 + -1.1 1,242,505 + +0.9 1,242,505 + +0.5 1,242,506 - -0.9 1,242,506 - +0.6 1,242,585 + +0.3 1,242,639 + -0.8 1,242,703 - +0.0 1,242,703 - -0.5 1,242,802 + -0.5 1,242,802 + -0.2 1,242,802 + +0.1 1,242,802 + +1.2 1,242,802 + +1.0 1,242,803 - -1.1 1,242,803 - -0.8 1,242,803 - -0.8 1,242,899 + ABZR87_RS05830 0.11 +0.6 1,242,899 + ABZR87_RS05830 0.11 +2.8 1,242,899 + ABZR87_RS05830 0.11 +1.1 1,242,899 + ABZR87_RS05830 0.11 -0.6 1,242,899 + ABZR87_RS05830 0.11 -0.8 1,242,899 + ABZR87_RS05830 0.11 -0.2 1,242,899 + ABZR87_RS05830 0.11 -0.8 1,242,899 + ABZR87_RS05830 0.11 +0.1 1,242,900 - ABZR87_RS05830 0.11 -0.4 1,242,900 - ABZR87_RS05830 0.11 +0.5 1,242,900 - ABZR87_RS05830 0.11 -0.3 1,242,900 - ABZR87_RS05830 0.11 -0.1 1,242,900 - ABZR87_RS05830 0.11 -0.0 1,242,926 + ABZR87_RS05830 0.13 -1.2 1,242,926 + ABZR87_RS05830 0.13 -0.7 1,242,926 + ABZR87_RS05830 0.13 +0.8 1,242,926 + ABZR87_RS05830 0.13 -0.6 1,242,926 + ABZR87_RS05830 0.13 -0.3 1,242,926 + ABZR87_RS05830 0.13 +0.5 1,242,926 + ABZR87_RS05830 0.13 -1.2 1,242,927 - ABZR87_RS05830 0.14 -0.3 1,242,927 - ABZR87_RS05830 0.14 -0.2 1,242,927 - ABZR87_RS05830 0.14 +0.2 1,242,927 - ABZR87_RS05830 0.14 +0.5 1,242,927 - ABZR87_RS05830 0.14 -0.6 1,243,001 + ABZR87_RS05830 0.20 -0.5 1,243,001 + ABZR87_RS05830 0.20 +0.0 1,243,002 - ABZR87_RS05830 0.20 +0.4 1,243,002 - ABZR87_RS05830 0.20 +0.1 1,243,002 - ABZR87_RS05830 0.20 +0.0 1,243,002 - ABZR87_RS05830 0.20 -0.6 1,243,002 - ABZR87_RS05830 0.20 -0.6 1,243,038 - ABZR87_RS05830 0.23 -2.2 1,243,038 - ABZR87_RS05830 0.23 +0.3 1,243,196 + ABZR87_RS05830 0.37 -0.2 1,243,196 + ABZR87_RS05830 0.37 +1.8 1,243,196 + ABZR87_RS05830 0.37 -0.1 1,243,196 + ABZR87_RS05830 0.37 -0.2 1,243,197 - ABZR87_RS05830 0.38 -0.2 1,243,197 - ABZR87_RS05830 0.38 +0.3 1,243,199 + ABZR87_RS05830 0.38 +0.6 1,243,200 - ABZR87_RS05830 0.38 +0.4 1,243,200 - ABZR87_RS05830 0.38 -0.6 1,243,200 - ABZR87_RS05830 0.38 -0.5 1,243,228 - ABZR87_RS05830 0.40 -0.7 1,243,228 - ABZR87_RS05830 0.40 -1.0 1,243,229 + ABZR87_RS05830 0.40 -0.2 1,243,229 + ABZR87_RS05830 0.40 +0.2 1,243,230 - ABZR87_RS05830 0.40 +0.1 1,243,322 + ABZR87_RS05830 0.49 +0.3 1,243,322 + ABZR87_RS05830 0.49 -1.4 1,243,322 + ABZR87_RS05830 0.49 -1.5 1,243,323 - ABZR87_RS05830 0.49 +0.6 1,243,323 - ABZR87_RS05830 0.49 -0.5 1,243,385 + ABZR87_RS05830 0.54 -0.4 1,243,385 + ABZR87_RS05830 0.54 +1.5 1,243,385 + ABZR87_RS05830 0.54 -0.1 1,243,386 - ABZR87_RS05830 0.54 -0.5 1,243,386 - ABZR87_RS05830 0.54 +0.2 1,243,424 + ABZR87_RS05830 0.58 -2.5 1,243,424 + ABZR87_RS05830 0.58 -0.5 1,243,424 + ABZR87_RS05830 0.58 -1.4 1,243,443 - ABZR87_RS05830 0.59 -0.0 1,243,473 + ABZR87_RS05830 0.62 -0.1 1,243,473 + ABZR87_RS05830 0.62 +0.1 1,243,473 + ABZR87_RS05830 0.62 -0.5 1,243,473 + ABZR87_RS05830 0.62 +0.4 1,243,473 + ABZR87_RS05830 0.62 +0.7 1,243,473 + ABZR87_RS05830 0.62 -0.2 1,243,473 + ABZR87_RS05830 0.62 +1.1 1,243,474 - ABZR87_RS05830 0.62 +0.6 1,243,474 - ABZR87_RS05830 0.62 +0.3 1,243,474 - ABZR87_RS05830 0.62 +0.3 1,243,474 - ABZR87_RS05830 0.62 -1.5 1,243,484 + ABZR87_RS05830 0.63 -0.6 1,243,484 + ABZR87_RS05830 0.63 +0.7 1,243,485 - ABZR87_RS05830 0.63 +0.3 1,243,485 - ABZR87_RS05830 0.63 +1.0 1,243,526 + ABZR87_RS05830 0.67 -1.4 1,243,527 - ABZR87_RS05830 0.67 +0.8 1,243,635 + ABZR87_RS05830 0.76 -0.4 1,243,774 - ABZR87_RS05830 0.89 +0.2 1,243,791 - +0.6 1,243,911 + +0.6 1,243,911 + +0.8 1,243,912 - -0.4 1,243,914 + +0.6 1,243,914 + +0.4 1,243,915 - +3.4 1,243,915 - -0.2 1,243,915 - -0.7 1,243,915 - +0.1 1,243,925 + +0.9 1,243,926 - +0.8 1,243,926 - -1.4 1,244,254 + -0.3 1,244,254 + +0.7 1,244,255 - -0.8 1,244,255 - +0.0 1,244,371 + ABZR87_RS05835 0.23 +0.1 1,244,372 - ABZR87_RS05835 0.23 -0.7 1,244,372 - ABZR87_RS05835 0.23 +0.4 1,244,481 - ABZR87_RS05835 0.39 -0.4 1,244,583 + ABZR87_RS05835 0.55 -0.4 1,244,647 - ABZR87_RS05835 0.64 -1.4 1,244,647 - ABZR87_RS05835 0.64 +0.0 1,244,805 + ABZR87_RS05835 0.88 +0.3 1,244,805 + ABZR87_RS05835 0.88 -0.7 1,244,805 + ABZR87_RS05835 0.88 -0.5 1,244,805 + ABZR87_RS05835 0.88 +0.0 1,244,805 + ABZR87_RS05835 0.88 -0.1 1,244,805 + ABZR87_RS05835 0.88 +0.6 1,244,805 + ABZR87_RS05835 0.88 -0.7 1,244,805 + ABZR87_RS05835 0.88 -1.3 1,244,805 + ABZR87_RS05835 0.88 +0.8 1,244,805 + ABZR87_RS05835 0.88 -0.2 1,244,805 + ABZR87_RS05835 0.88 -0.9 1,244,805 + ABZR87_RS05835 0.88 -0.0 1,244,805 + ABZR87_RS05835 0.88 -1.3 1,244,805 + ABZR87_RS05835 0.88 -0.8 1,244,806 - ABZR87_RS05835 0.88 -0.4 1,244,806 - ABZR87_RS05835 0.88 -0.0 1,244,806 - ABZR87_RS05835 0.88 -0.0 1,244,806 - ABZR87_RS05835 0.88 -1.0 1,244,810 + ABZR87_RS05835 0.89 -0.6 1,244,810 + ABZR87_RS05835 0.89 -1.6 1,244,811 - ABZR87_RS05835 0.89 -0.4 1,244,835 + -0.5 1,244,835 + -0.4 1,244,836 - -1.1
Or see this region's nucleotide sequence