Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS05585 and ABZR87_RS05590 are separated by 600 nucleotides ABZR87_RS05590 and ABZR87_RS05595 are separated by 32 nucleotides ABZR87_RS05595 and ABZR87_RS05600 are separated by 127 nucleotides
ABZR87_RS05585: ABZR87_RS05585 - polyphosphate kinase 2 family protein, at 1,187,086 to 1,187,913
_RS05585
ABZR87_RS05590: ABZR87_RS05590 - orotate phosphoribosyltransferase, at 1,188,514 to 1,189,191
_RS05590
ABZR87_RS05595: ABZR87_RS05595 - exodeoxyribonuclease III, at 1,189,224 to 1,190,009
_RS05595
ABZR87_RS05600: ABZR87_RS05600 - N-acetyl-gamma-glutamyl-phosphate reductase, at 1,190,137 to 1,191,150
_RS05600
Position (kb)
1188
1189
1190 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1187.546 kb on - strand, within ABZR87_RS05585 at 1187.616 kb on + strand, within ABZR87_RS05585 at 1187.616 kb on + strand, within ABZR87_RS05585 at 1187.616 kb on + strand, within ABZR87_RS05585 at 1187.617 kb on - strand, within ABZR87_RS05585 at 1187.617 kb on - strand, within ABZR87_RS05585 at 1187.617 kb on - strand, within ABZR87_RS05585 at 1187.617 kb on - strand, within ABZR87_RS05585 at 1187.617 kb on - strand, within ABZR87_RS05585 at 1187.617 kb on - strand, within ABZR87_RS05585 at 1187.697 kb on + strand, within ABZR87_RS05585 at 1187.697 kb on + strand, within ABZR87_RS05585 at 1187.698 kb on - strand, within ABZR87_RS05585 at 1187.725 kb on + strand, within ABZR87_RS05585 at 1187.725 kb on + strand, within ABZR87_RS05585 at 1187.725 kb on + strand, within ABZR87_RS05585 at 1187.725 kb on + strand, within ABZR87_RS05585 at 1187.725 kb on + strand, within ABZR87_RS05585 at 1187.725 kb on + strand, within ABZR87_RS05585 at 1187.725 kb on + strand, within ABZR87_RS05585 at 1187.725 kb on + strand, within ABZR87_RS05585 at 1187.725 kb on + strand, within ABZR87_RS05585 at 1187.725 kb on + strand, within ABZR87_RS05585 at 1187.725 kb on + strand, within ABZR87_RS05585 at 1187.725 kb on + strand, within ABZR87_RS05585 at 1187.725 kb on + strand, within ABZR87_RS05585 at 1187.726 kb on - strand, within ABZR87_RS05585 at 1187.726 kb on - strand, within ABZR87_RS05585 at 1187.726 kb on - strand, within ABZR87_RS05585 at 1187.726 kb on - strand, within ABZR87_RS05585 at 1187.726 kb on - strand, within ABZR87_RS05585 at 1187.726 kb on - strand, within ABZR87_RS05585 at 1187.726 kb on - strand, within ABZR87_RS05585 at 1187.726 kb on - strand, within ABZR87_RS05585 at 1187.726 kb on - strand, within ABZR87_RS05585 at 1187.726 kb on - strand, within ABZR87_RS05585 at 1187.726 kb on - strand, within ABZR87_RS05585 at 1187.726 kb on - strand, within ABZR87_RS05585 at 1187.726 kb on - strand, within ABZR87_RS05585 at 1187.727 kb on + strand, within ABZR87_RS05585 at 1187.727 kb on + strand, within ABZR87_RS05585 at 1187.727 kb on + strand, within ABZR87_RS05585 at 1187.727 kb on + strand, within ABZR87_RS05585 at 1187.727 kb on + strand, within ABZR87_RS05585 at 1187.727 kb on + strand, within ABZR87_RS05585 at 1187.727 kb on + strand, within ABZR87_RS05585 at 1187.727 kb on + strand, within ABZR87_RS05585 at 1187.728 kb on - strand, within ABZR87_RS05585 at 1187.728 kb on - strand, within ABZR87_RS05585 at 1187.728 kb on - strand, within ABZR87_RS05585 at 1187.737 kb on + strand, within ABZR87_RS05585 at 1187.737 kb on + strand, within ABZR87_RS05585 at 1187.737 kb on + strand, within ABZR87_RS05585 at 1187.737 kb on + strand, within ABZR87_RS05585 at 1187.738 kb on - strand, within ABZR87_RS05585 at 1187.738 kb on - strand, within ABZR87_RS05585 at 1187.738 kb on - strand, within ABZR87_RS05585 at 1187.782 kb on + strand, within ABZR87_RS05585 at 1187.782 kb on + strand, within ABZR87_RS05585 at 1187.782 kb on + strand, within ABZR87_RS05585 at 1187.782 kb on + strand, within ABZR87_RS05585 at 1187.782 kb on + strand, within ABZR87_RS05585 at 1187.782 kb on + strand, within ABZR87_RS05585 at 1187.782 kb on + strand, within ABZR87_RS05585 at 1187.782 kb on + strand, within ABZR87_RS05585 at 1187.782 kb on + strand, within ABZR87_RS05585 at 1187.782 kb on + strand, within ABZR87_RS05585 at 1187.782 kb on + strand, within ABZR87_RS05585 at 1187.783 kb on - strand, within ABZR87_RS05585 at 1187.783 kb on - strand, within ABZR87_RS05585 at 1187.783 kb on - strand, within ABZR87_RS05585 at 1187.783 kb on - strand, within ABZR87_RS05585 at 1187.783 kb on - strand, within ABZR87_RS05585 at 1187.783 kb on - strand, within ABZR87_RS05585 at 1187.783 kb on - strand, within ABZR87_RS05585 at 1187.783 kb on - strand, within ABZR87_RS05585 at 1187.783 kb on - strand, within ABZR87_RS05585 at 1187.783 kb on - strand, within ABZR87_RS05585 at 1187.869 kb on + strand at 1187.869 kb on + strand at 1188.256 kb on - strand at 1188.256 kb on - strand at 1188.533 kb on + strand at 1188.533 kb on + strand at 1188.534 kb on - strand at 1188.534 kb on - strand at 1188.534 kb on - strand at 1188.537 kb on + strand at 1188.537 kb on + strand at 1188.537 kb on + strand at 1188.537 kb on + strand at 1188.537 kb on + strand at 1188.538 kb on - strand at 1188.567 kb on - strand at 1188.602 kb on + strand, within ABZR87_RS05590 at 1188.603 kb on - strand, within ABZR87_RS05590 at 1188.624 kb on + strand, within ABZR87_RS05590 at 1188.690 kb on + strand, within ABZR87_RS05590 at 1188.690 kb on + strand, within ABZR87_RS05590 at 1188.896 kb on + strand, within ABZR87_RS05590 at 1188.968 kb on + strand, within ABZR87_RS05590 at 1188.969 kb on - strand, within ABZR87_RS05590 at 1189.025 kb on + strand, within ABZR87_RS05590 at 1189.026 kb on - strand, within ABZR87_RS05590 at 1189.086 kb on - strand, within ABZR87_RS05590 at 1189.228 kb on + strand at 1189.229 kb on - strand at 1189.229 kb on - strand at 1189.229 kb on - strand at 1189.229 kb on - strand at 1189.383 kb on + strand, within ABZR87_RS05595 at 1189.383 kb on + strand, within ABZR87_RS05595 at 1189.384 kb on - strand, within ABZR87_RS05595 at 1189.419 kb on + strand, within ABZR87_RS05595 at 1189.420 kb on - strand, within ABZR87_RS05595 at 1189.437 kb on + strand, within ABZR87_RS05595 at 1189.437 kb on + strand, within ABZR87_RS05595 at 1189.437 kb on + strand, within ABZR87_RS05595 at 1189.437 kb on + strand, within ABZR87_RS05595 at 1189.437 kb on + strand, within ABZR87_RS05595 at 1189.437 kb on + strand, within ABZR87_RS05595 at 1189.437 kb on + strand, within ABZR87_RS05595 at 1189.438 kb on - strand, within ABZR87_RS05595 at 1189.438 kb on - strand, within ABZR87_RS05595 at 1189.438 kb on - strand, within ABZR87_RS05595 at 1189.438 kb on - strand, within ABZR87_RS05595 at 1189.506 kb on + strand, within ABZR87_RS05595 at 1189.548 kb on + strand, within ABZR87_RS05595 at 1189.548 kb on + strand, within ABZR87_RS05595 at 1189.549 kb on - strand, within ABZR87_RS05595 at 1189.549 kb on - strand, within ABZR87_RS05595 at 1189.788 kb on + strand, within ABZR87_RS05595 at 1189.788 kb on + strand, within ABZR87_RS05595 at 1189.789 kb on - strand, within ABZR87_RS05595 at 1189.789 kb on - strand, within ABZR87_RS05595 at 1189.789 kb on - strand, within ABZR87_RS05595 at 1189.789 kb on - strand, within ABZR87_RS05595 at 1189.789 kb on - strand, within ABZR87_RS05595 at 1189.830 kb on + strand, within ABZR87_RS05595 at 1189.830 kb on + strand, within ABZR87_RS05595 at 1189.830 kb on + strand, within ABZR87_RS05595 at 1189.830 kb on + strand, within ABZR87_RS05595 at 1189.830 kb on + strand, within ABZR87_RS05595 at 1189.830 kb on + strand, within ABZR87_RS05595 at 1189.830 kb on + strand, within ABZR87_RS05595 at 1189.830 kb on + strand, within ABZR87_RS05595 at 1189.830 kb on + strand, within ABZR87_RS05595 at 1189.830 kb on + strand, within ABZR87_RS05595 at 1189.831 kb on - strand, within ABZR87_RS05595 at 1189.831 kb on - strand, within ABZR87_RS05595 at 1189.831 kb on - strand, within ABZR87_RS05595 at 1189.831 kb on - strand, within ABZR87_RS05595 at 1189.831 kb on - strand, within ABZR87_RS05595 at 1189.831 kb on - strand, within ABZR87_RS05595 at 1189.860 kb on + strand, within ABZR87_RS05595 at 1189.860 kb on + strand, within ABZR87_RS05595 at 1189.860 kb on + strand, within ABZR87_RS05595 at 1189.861 kb on - strand, within ABZR87_RS05595 at 1189.861 kb on - strand, within ABZR87_RS05595 at 1189.861 kb on - strand, within ABZR87_RS05595 at 1189.861 kb on - strand, within ABZR87_RS05595 at 1189.861 kb on - strand, within ABZR87_RS05595 at 1189.883 kb on + strand, within ABZR87_RS05595 at 1189.883 kb on + strand, within ABZR87_RS05595 at 1189.884 kb on - strand, within ABZR87_RS05595 at 1189.986 kb on + strand at 1189.986 kb on + strand at 1189.987 kb on - strand at 1189.987 kb on - strand at 1189.987 kb on - strand at 1190.008 kb on - strand at 1190.008 kb on - strand at 1190.063 kb on + strand at 1190.063 kb on + strand at 1190.064 kb on - strand at 1190.064 kb on - strand at 1190.064 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 1,187,546 - ABZR87_RS05585 0.56 -1.2 1,187,616 + ABZR87_RS05585 0.64 +1.1 1,187,616 + ABZR87_RS05585 0.64 -0.1 1,187,616 + ABZR87_RS05585 0.64 -0.7 1,187,617 - ABZR87_RS05585 0.64 +0.0 1,187,617 - ABZR87_RS05585 0.64 -0.7 1,187,617 - ABZR87_RS05585 0.64 -0.9 1,187,617 - ABZR87_RS05585 0.64 -1.4 1,187,617 - ABZR87_RS05585 0.64 -0.3 1,187,617 - ABZR87_RS05585 0.64 -0.0 1,187,697 + ABZR87_RS05585 0.74 -0.0 1,187,697 + ABZR87_RS05585 0.74 +0.6 1,187,698 - ABZR87_RS05585 0.74 -0.8 1,187,725 + ABZR87_RS05585 0.77 +0.0 1,187,725 + ABZR87_RS05585 0.77 -0.3 1,187,725 + ABZR87_RS05585 0.77 +0.2 1,187,725 + ABZR87_RS05585 0.77 +0.1 1,187,725 + ABZR87_RS05585 0.77 -1.8 1,187,725 + ABZR87_RS05585 0.77 -0.2 1,187,725 + ABZR87_RS05585 0.77 -0.7 1,187,725 + ABZR87_RS05585 0.77 +0.1 1,187,725 + ABZR87_RS05585 0.77 -0.7 1,187,725 + ABZR87_RS05585 0.77 -2.5 1,187,725 + ABZR87_RS05585 0.77 +0.8 1,187,725 + ABZR87_RS05585 0.77 +2.8 1,187,725 + ABZR87_RS05585 0.77 -1.5 1,187,726 - ABZR87_RS05585 0.77 -1.7 1,187,726 - ABZR87_RS05585 0.77 -0.1 1,187,726 - ABZR87_RS05585 0.77 +0.2 1,187,726 - ABZR87_RS05585 0.77 +0.7 1,187,726 - ABZR87_RS05585 0.77 +0.0 1,187,726 - ABZR87_RS05585 0.77 -0.1 1,187,726 - ABZR87_RS05585 0.77 -0.7 1,187,726 - ABZR87_RS05585 0.77 -0.6 1,187,726 - ABZR87_RS05585 0.77 -0.2 1,187,726 - ABZR87_RS05585 0.77 +1.3 1,187,726 - ABZR87_RS05585 0.77 -0.2 1,187,726 - ABZR87_RS05585 0.77 -1.1 1,187,726 - ABZR87_RS05585 0.77 +0.8 1,187,727 + ABZR87_RS05585 0.77 -0.3 1,187,727 + ABZR87_RS05585 0.77 +0.3 1,187,727 + ABZR87_RS05585 0.77 +0.3 1,187,727 + ABZR87_RS05585 0.77 +0.0 1,187,727 + ABZR87_RS05585 0.77 -0.4 1,187,727 + ABZR87_RS05585 0.77 -0.2 1,187,727 + ABZR87_RS05585 0.77 +0.3 1,187,727 + ABZR87_RS05585 0.77 +1.4 1,187,728 - ABZR87_RS05585 0.78 -0.1 1,187,728 - ABZR87_RS05585 0.78 +1.4 1,187,728 - ABZR87_RS05585 0.78 -0.1 1,187,737 + ABZR87_RS05585 0.79 -1.7 1,187,737 + ABZR87_RS05585 0.79 +0.0 1,187,737 + ABZR87_RS05585 0.79 -0.4 1,187,737 + ABZR87_RS05585 0.79 -0.5 1,187,738 - ABZR87_RS05585 0.79 +0.6 1,187,738 - ABZR87_RS05585 0.79 -1.0 1,187,738 - ABZR87_RS05585 0.79 -0.4 1,187,782 + ABZR87_RS05585 0.84 -0.2 1,187,782 + ABZR87_RS05585 0.84 -2.7 1,187,782 + ABZR87_RS05585 0.84 -0.9 1,187,782 + ABZR87_RS05585 0.84 -0.4 1,187,782 + ABZR87_RS05585 0.84 +0.4 1,187,782 + ABZR87_RS05585 0.84 +0.5 1,187,782 + ABZR87_RS05585 0.84 -0.1 1,187,782 + ABZR87_RS05585 0.84 +0.2 1,187,782 + ABZR87_RS05585 0.84 +0.2 1,187,782 + ABZR87_RS05585 0.84 +1.1 1,187,782 + ABZR87_RS05585 0.84 +0.3 1,187,783 - ABZR87_RS05585 0.84 -1.8 1,187,783 - ABZR87_RS05585 0.84 -0.3 1,187,783 - ABZR87_RS05585 0.84 -0.6 1,187,783 - ABZR87_RS05585 0.84 -0.6 1,187,783 - ABZR87_RS05585 0.84 -1.6 1,187,783 - ABZR87_RS05585 0.84 +0.4 1,187,783 - ABZR87_RS05585 0.84 -1.5 1,187,783 - ABZR87_RS05585 0.84 +0.3 1,187,783 - ABZR87_RS05585 0.84 +0.1 1,187,783 - ABZR87_RS05585 0.84 +0.0 1,187,869 + +1.5 1,187,869 + -1.1 1,188,256 - +0.1 1,188,256 - -0.2 1,188,533 + -1.7 1,188,533 + -1.5 1,188,534 - -0.9 1,188,534 - -0.6 1,188,534 - -0.5 1,188,537 + -0.1 1,188,537 + +0.9 1,188,537 + -1.8 1,188,537 + -0.2 1,188,537 + -1.0 1,188,538 - -0.7 1,188,567 - -1.4 1,188,602 + ABZR87_RS05590 0.13 +1.8 1,188,603 - ABZR87_RS05590 0.13 -0.8 1,188,624 + ABZR87_RS05590 0.16 +1.8 1,188,690 + ABZR87_RS05590 0.26 +0.8 1,188,690 + ABZR87_RS05590 0.26 -0.2 1,188,896 + ABZR87_RS05590 0.56 +0.8 1,188,968 + ABZR87_RS05590 0.67 +0.8 1,188,969 - ABZR87_RS05590 0.67 +1.8 1,189,025 + ABZR87_RS05590 0.75 +0.8 1,189,026 - ABZR87_RS05590 0.76 -1.0 1,189,086 - ABZR87_RS05590 0.84 +1.8 1,189,228 + -1.3 1,189,229 - -0.7 1,189,229 - +0.8 1,189,229 - -1.4 1,189,229 - +0.5 1,189,383 + ABZR87_RS05595 0.20 -0.3 1,189,383 + ABZR87_RS05595 0.20 -1.4 1,189,384 - ABZR87_RS05595 0.20 -0.5 1,189,419 + ABZR87_RS05595 0.25 -1.1 1,189,420 - ABZR87_RS05595 0.25 +0.8 1,189,437 + ABZR87_RS05595 0.27 -0.9 1,189,437 + ABZR87_RS05595 0.27 -0.7 1,189,437 + ABZR87_RS05595 0.27 +0.0 1,189,437 + ABZR87_RS05595 0.27 +0.0 1,189,437 + ABZR87_RS05595 0.27 -1.2 1,189,437 + ABZR87_RS05595 0.27 +0.6 1,189,437 + ABZR87_RS05595 0.27 -0.3 1,189,438 - ABZR87_RS05595 0.27 -0.1 1,189,438 - ABZR87_RS05595 0.27 +0.6 1,189,438 - ABZR87_RS05595 0.27 -2.2 1,189,438 - ABZR87_RS05595 0.27 -1.1 1,189,506 + ABZR87_RS05595 0.36 +0.2 1,189,548 + ABZR87_RS05595 0.41 -0.3 1,189,548 + ABZR87_RS05595 0.41 +0.2 1,189,549 - ABZR87_RS05595 0.41 +0.2 1,189,549 - ABZR87_RS05595 0.41 +0.4 1,189,788 + ABZR87_RS05595 0.72 +0.4 1,189,788 + ABZR87_RS05595 0.72 -0.0 1,189,789 - ABZR87_RS05595 0.72 -1.9 1,189,789 - ABZR87_RS05595 0.72 +0.4 1,189,789 - ABZR87_RS05595 0.72 -0.5 1,189,789 - ABZR87_RS05595 0.72 +0.2 1,189,789 - ABZR87_RS05595 0.72 +0.1 1,189,830 + ABZR87_RS05595 0.77 -0.2 1,189,830 + ABZR87_RS05595 0.77 +0.9 1,189,830 + ABZR87_RS05595 0.77 +0.3 1,189,830 + ABZR87_RS05595 0.77 +0.6 1,189,830 + ABZR87_RS05595 0.77 -0.6 1,189,830 + ABZR87_RS05595 0.77 -1.6 1,189,830 + ABZR87_RS05595 0.77 +1.3 1,189,830 + ABZR87_RS05595 0.77 -1.0 1,189,830 + ABZR87_RS05595 0.77 -1.2 1,189,830 + ABZR87_RS05595 0.77 -0.4 1,189,831 - ABZR87_RS05595 0.77 +1.2 1,189,831 - ABZR87_RS05595 0.77 +0.2 1,189,831 - ABZR87_RS05595 0.77 -0.1 1,189,831 - ABZR87_RS05595 0.77 -0.8 1,189,831 - ABZR87_RS05595 0.77 -0.4 1,189,831 - ABZR87_RS05595 0.77 +0.3 1,189,860 + ABZR87_RS05595 0.81 -0.4 1,189,860 + ABZR87_RS05595 0.81 -1.2 1,189,860 + ABZR87_RS05595 0.81 +0.3 1,189,861 - ABZR87_RS05595 0.81 +0.2 1,189,861 - ABZR87_RS05595 0.81 +0.5 1,189,861 - ABZR87_RS05595 0.81 +1.8 1,189,861 - ABZR87_RS05595 0.81 -0.7 1,189,861 - ABZR87_RS05595 0.81 -0.8 1,189,883 + ABZR87_RS05595 0.84 -0.6 1,189,883 + ABZR87_RS05595 0.84 +0.0 1,189,884 - ABZR87_RS05595 0.84 -0.1 1,189,986 + -0.9 1,189,986 + -2.0 1,189,987 - +0.0 1,189,987 - +0.2 1,189,987 - +0.0 1,190,008 - +0.8 1,190,008 - -2.0 1,190,063 + -0.4 1,190,063 + +0.1 1,190,064 - +0.3 1,190,064 - -0.9 1,190,064 - -0.5
Or see this region's nucleotide sequence