Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS03700 and ABZR87_RS03705 are separated by 1 nucleotides ABZR87_RS03705 and ABZR87_RS03710 are separated by 313 nucleotides ABZR87_RS03710 and ABZR87_RS03715 are separated by 91 nucleotides ABZR87_RS03715 and ABZR87_RS03720 are separated by 46 nucleotides
ABZR87_RS03700: ABZR87_RS03700 - RNA polymerase sigma factor, at 770,282 to 770,839
_RS03700
ABZR87_RS03705: ABZR87_RS03705 - DUF2892 domain-containing protein, at 770,841 to 771,050
_RS03705
ABZR87_RS03710: ABZR87_RS03710 - Hsp70 family protein, at 771,364 to 772,617
_RS03710
ABZR87_RS03715: ABZR87_RS03715 - NADPH-dependent FMN reductase, at 772,709 to 773,275
_RS03715
ABZR87_RS03720: ABZR87_RS03720 - hypothetical protein, at 773,322 to 773,690
_RS03720
Position (kb) 771
772
773 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 770.399 kb on + strand, within ABZR87_RS03700 at 770.399 kb on + strand, within ABZR87_RS03700 at 770.399 kb on + strand, within ABZR87_RS03700 at 770.399 kb on + strand, within ABZR87_RS03700 at 770.400 kb on - strand, within ABZR87_RS03700 at 770.400 kb on - strand, within ABZR87_RS03700 at 770.400 kb on - strand, within ABZR87_RS03700 at 770.464 kb on - strand, within ABZR87_RS03700 at 770.464 kb on - strand, within ABZR87_RS03700 at 770.464 kb on - strand, within ABZR87_RS03700 at 770.464 kb on - strand, within ABZR87_RS03700 at 770.464 kb on - strand, within ABZR87_RS03700 at 770.464 kb on - strand, within ABZR87_RS03700 at 770.464 kb on - strand, within ABZR87_RS03700 at 770.556 kb on + strand, within ABZR87_RS03700 at 770.556 kb on + strand, within ABZR87_RS03700 at 770.556 kb on + strand, within ABZR87_RS03700 at 770.557 kb on - strand, within ABZR87_RS03700 at 770.584 kb on + strand, within ABZR87_RS03700 at 770.585 kb on - strand, within ABZR87_RS03700 at 770.621 kb on + strand, within ABZR87_RS03700 at 770.622 kb on - strand, within ABZR87_RS03700 at 770.622 kb on - strand, within ABZR87_RS03700 at 770.652 kb on - strand, within ABZR87_RS03700 at 770.671 kb on - strand, within ABZR87_RS03700 at 770.747 kb on + strand, within ABZR87_RS03700 at 770.747 kb on + strand, within ABZR87_RS03700 at 770.795 kb on - strand at 770.815 kb on + strand at 770.815 kb on + strand at 770.815 kb on + strand at 770.815 kb on + strand at 770.815 kb on + strand at 770.815 kb on + strand at 770.815 kb on + strand at 770.816 kb on - strand at 770.816 kb on - strand at 770.816 kb on - strand at 770.816 kb on - strand at 770.816 kb on - strand at 770.850 kb on + strand at 770.851 kb on - strand at 770.972 kb on + strand, within ABZR87_RS03705 at 770.973 kb on - strand, within ABZR87_RS03705 at 771.043 kb on + strand at 771.043 kb on + strand at 771.043 kb on + strand at 771.043 kb on + strand at 771.044 kb on - strand at 771.044 kb on - strand at 771.044 kb on - strand at 771.050 kb on + strand at 771.050 kb on + strand at 771.050 kb on + strand at 771.050 kb on + strand at 771.050 kb on + strand at 771.050 kb on + strand at 771.050 kb on + strand at 771.050 kb on + strand at 771.050 kb on + strand at 771.050 kb on + strand at 771.051 kb on - strand at 771.051 kb on - strand at 771.051 kb on - strand at 771.051 kb on - strand at 771.051 kb on - strand at 771.051 kb on - strand at 771.051 kb on - strand at 771.051 kb on - strand at 771.051 kb on - strand at 771.051 kb on - strand at 771.088 kb on + strand at 771.088 kb on + strand at 771.088 kb on + strand at 771.088 kb on + strand at 771.089 kb on - strand at 771.089 kb on - strand at 771.089 kb on - strand at 771.089 kb on - strand at 771.089 kb on - strand at 771.089 kb on - strand at 771.125 kb on + strand at 771.129 kb on + strand at 771.130 kb on - strand at 771.130 kb on - strand at 771.130 kb on - strand at 771.130 kb on - strand at 771.130 kb on - strand at 771.152 kb on + strand at 771.152 kb on + strand at 771.152 kb on + strand at 771.152 kb on + strand at 771.152 kb on + strand at 771.152 kb on + strand at 771.152 kb on + strand at 771.152 kb on + strand at 771.152 kb on + strand at 771.152 kb on + strand at 771.152 kb on + strand at 771.153 kb on - strand at 771.153 kb on - strand at 771.153 kb on - strand at 771.153 kb on - strand at 771.153 kb on - strand at 771.153 kb on - strand at 771.153 kb on - strand at 771.153 kb on - strand at 771.153 kb on - strand at 771.153 kb on - strand at 771.153 kb on - strand at 771.153 kb on - strand at 771.153 kb on - strand at 771.153 kb on - strand at 771.153 kb on - strand at 771.198 kb on + strand at 771.198 kb on + strand at 771.198 kb on + strand at 771.198 kb on + strand at 771.198 kb on + strand at 771.199 kb on - strand at 771.199 kb on - strand at 771.199 kb on - strand at 771.199 kb on - strand at 771.199 kb on - strand at 771.199 kb on - strand at 771.275 kb on + strand at 771.275 kb on + strand at 771.275 kb on + strand at 771.276 kb on - strand at 771.276 kb on - strand at 771.276 kb on - strand at 771.276 kb on - strand at 771.276 kb on - strand at 771.280 kb on + strand at 771.280 kb on + strand at 771.280 kb on + strand at 771.280 kb on + strand at 771.281 kb on - strand at 771.314 kb on + strand at 771.363 kb on + strand at 771.363 kb on + strand at 771.363 kb on + strand at 771.363 kb on + strand at 771.363 kb on + strand at 771.364 kb on - strand at 771.364 kb on - strand at 771.364 kb on - strand at 771.517 kb on + strand, within ABZR87_RS03710 at 771.517 kb on + strand, within ABZR87_RS03710 at 771.518 kb on - strand, within ABZR87_RS03710 at 771.541 kb on + strand, within ABZR87_RS03710 at 771.541 kb on + strand, within ABZR87_RS03710 at 771.542 kb on - strand, within ABZR87_RS03710 at 771.554 kb on - strand, within ABZR87_RS03710 at 771.839 kb on - strand, within ABZR87_RS03710 at 771.839 kb on - strand, within ABZR87_RS03710 at 771.865 kb on + strand, within ABZR87_RS03710 at 771.865 kb on + strand, within ABZR87_RS03710 at 771.866 kb on - strand, within ABZR87_RS03710 at 771.921 kb on + strand, within ABZR87_RS03710 at 771.922 kb on - strand, within ABZR87_RS03710 at 771.922 kb on - strand, within ABZR87_RS03710 at 772.064 kb on + strand, within ABZR87_RS03710 at 772.117 kb on + strand, within ABZR87_RS03710 at 772.118 kb on - strand, within ABZR87_RS03710 at 772.156 kb on + strand, within ABZR87_RS03710 at 772.156 kb on + strand, within ABZR87_RS03710 at 772.156 kb on + strand, within ABZR87_RS03710 at 772.157 kb on - strand, within ABZR87_RS03710 at 772.157 kb on - strand, within ABZR87_RS03710 at 772.157 kb on - strand, within ABZR87_RS03710 at 772.157 kb on - strand, within ABZR87_RS03710 at 772.157 kb on - strand, within ABZR87_RS03710 at 772.193 kb on - strand, within ABZR87_RS03710 at 772.193 kb on - strand, within ABZR87_RS03710 at 772.193 kb on - strand, within ABZR87_RS03710 at 772.193 kb on - strand, within ABZR87_RS03710 at 772.193 kb on - strand, within ABZR87_RS03710 at 772.193 kb on - strand, within ABZR87_RS03710 at 772.468 kb on - strand, within ABZR87_RS03710 at 772.695 kb on + strand at 772.764 kb on + strand at 772.765 kb on - strand at 772.765 kb on - strand at 772.793 kb on - strand, within ABZR87_RS03715 at 772.806 kb on + strand, within ABZR87_RS03715 at 772.807 kb on - strand, within ABZR87_RS03715 at 772.812 kb on + strand, within ABZR87_RS03715 at 772.812 kb on + strand, within ABZR87_RS03715 at 772.812 kb on + strand, within ABZR87_RS03715 at 772.812 kb on + strand, within ABZR87_RS03715 at 772.812 kb on + strand, within ABZR87_RS03715 at 772.812 kb on + strand, within ABZR87_RS03715 at 772.812 kb on + strand, within ABZR87_RS03715 at 772.813 kb on - strand, within ABZR87_RS03715 at 772.813 kb on - strand, within ABZR87_RS03715 at 772.813 kb on - strand, within ABZR87_RS03715 at 772.924 kb on + strand, within ABZR87_RS03715 at 772.924 kb on + strand, within ABZR87_RS03715 at 772.924 kb on + strand, within ABZR87_RS03715 at 772.924 kb on + strand, within ABZR87_RS03715 at 772.925 kb on - strand, within ABZR87_RS03715 at 772.925 kb on - strand, within ABZR87_RS03715 at 772.925 kb on - strand, within ABZR87_RS03715 at 772.925 kb on - strand, within ABZR87_RS03715 at 772.925 kb on - strand, within ABZR87_RS03715 at 772.925 kb on - strand, within ABZR87_RS03715 at 772.941 kb on - strand, within ABZR87_RS03715 at 772.941 kb on - strand, within ABZR87_RS03715 at 773.323 kb on + strand at 773.323 kb on + strand at 773.324 kb on - strand at 773.324 kb on - strand at 773.324 kb on - strand at 773.324 kb on - strand at 773.333 kb on - strand at 773.333 kb on - strand at 773.346 kb on + strand at 773.347 kb on - strand at 773.355 kb on + strand at 773.355 kb on + strand at 773.355 kb on + strand at 773.355 kb on + strand at 773.355 kb on + strand at 773.355 kb on + strand at 773.355 kb on + strand at 773.356 kb on - strand at 773.356 kb on - strand at 773.356 kb on - strand at 773.356 kb on - strand at 773.356 kb on - strand at 773.358 kb on + strand at 773.358 kb on + strand at 773.358 kb on + strand at 773.358 kb on + strand at 773.358 kb on + strand at 773.359 kb on - strand, within ABZR87_RS03720 at 773.359 kb on - strand, within ABZR87_RS03720 at 773.359 kb on - strand, within ABZR87_RS03720 at 773.359 kb on - strand, within ABZR87_RS03720 at 773.359 kb on - strand, within ABZR87_RS03720 at 773.359 kb on - strand, within ABZR87_RS03720 at 773.359 kb on - strand, within ABZR87_RS03720 at 773.359 kb on - strand, within ABZR87_RS03720 at 773.359 kb on - strand, within ABZR87_RS03720 at 773.406 kb on + strand, within ABZR87_RS03720 at 773.406 kb on + strand, within ABZR87_RS03720 at 773.406 kb on + strand, within ABZR87_RS03720 at 773.406 kb on + strand, within ABZR87_RS03720 at 773.407 kb on - strand, within ABZR87_RS03720 at 773.410 kb on + strand, within ABZR87_RS03720 at 773.410 kb on + strand, within ABZR87_RS03720 at 773.411 kb on - strand, within ABZR87_RS03720 at 773.411 kb on - strand, within ABZR87_RS03720 at 773.411 kb on - strand, within ABZR87_RS03720 at 773.411 kb on - strand, within ABZR87_RS03720 at 773.429 kb on + strand, within ABZR87_RS03720 at 773.429 kb on + strand, within ABZR87_RS03720 at 773.441 kb on + strand, within ABZR87_RS03720 at 773.441 kb on + strand, within ABZR87_RS03720 at 773.441 kb on + strand, within ABZR87_RS03720 at 773.578 kb on + strand, within ABZR87_RS03720 at 773.582 kb on + strand, within ABZR87_RS03720 at 773.582 kb on + strand, within ABZR87_RS03720
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 770,399 + ABZR87_RS03700 0.21 +0.4 770,399 + ABZR87_RS03700 0.21 -0.5 770,399 + ABZR87_RS03700 0.21 +0.4 770,399 + ABZR87_RS03700 0.21 +1.2 770,400 - ABZR87_RS03700 0.21 -0.5 770,400 - ABZR87_RS03700 0.21 -0.3 770,400 - ABZR87_RS03700 0.21 -1.8 770,464 - ABZR87_RS03700 0.33 -1.9 770,464 - ABZR87_RS03700 0.33 +0.3 770,464 - ABZR87_RS03700 0.33 -1.1 770,464 - ABZR87_RS03700 0.33 +0.2 770,464 - ABZR87_RS03700 0.33 +1.2 770,464 - ABZR87_RS03700 0.33 -1.9 770,464 - ABZR87_RS03700 0.33 -1.3 770,556 + ABZR87_RS03700 0.49 +0.3 770,556 + ABZR87_RS03700 0.49 +0.1 770,556 + ABZR87_RS03700 0.49 -1.2 770,557 - ABZR87_RS03700 0.49 -0.2 770,584 + ABZR87_RS03700 0.54 +3.3 770,585 - ABZR87_RS03700 0.54 -0.7 770,621 + ABZR87_RS03700 0.61 +0.8 770,622 - ABZR87_RS03700 0.61 +0.7 770,622 - ABZR87_RS03700 0.61 +0.2 770,652 - ABZR87_RS03700 0.66 +0.3 770,671 - ABZR87_RS03700 0.70 +0.1 770,747 + ABZR87_RS03700 0.83 -0.7 770,747 + ABZR87_RS03700 0.83 -0.3 770,795 - -0.5 770,815 + +0.6 770,815 + -0.0 770,815 + -0.8 770,815 + +4.1 770,815 + -0.9 770,815 + +1.0 770,815 + +0.5 770,816 - -0.4 770,816 - +0.4 770,816 - -0.6 770,816 - +0.6 770,816 - +0.2 770,850 + +1.7 770,851 - +0.7 770,972 + ABZR87_RS03705 0.62 +1.6 770,973 - ABZR87_RS03705 0.63 -0.6 771,043 + +0.2 771,043 + -0.4 771,043 + -1.2 771,043 + -0.1 771,044 - +0.3 771,044 - +0.2 771,044 - +1.1 771,050 + +0.4 771,050 + -0.2 771,050 + +0.1 771,050 + +0.5 771,050 + +0.1 771,050 + -0.3 771,050 + -1.5 771,050 + -0.6 771,050 + -1.1 771,050 + +0.4 771,051 - -0.2 771,051 - -0.0 771,051 - +0.3 771,051 - -0.2 771,051 - +0.4 771,051 - +0.9 771,051 - -0.3 771,051 - -0.3 771,051 - +0.9 771,051 - -0.2 771,088 + +0.7 771,088 + -0.6 771,088 + -2.0 771,088 + -0.4 771,089 - -2.0 771,089 - +0.2 771,089 - -0.1 771,089 - -1.8 771,089 - +1.2 771,089 - -0.1 771,125 + +1.4 771,129 + -0.3 771,130 - -1.5 771,130 - +0.9 771,130 - -0.2 771,130 - -0.3 771,130 - -1.3 771,152 + -0.7 771,152 + +0.7 771,152 + -0.2 771,152 + -0.3 771,152 + +1.7 771,152 + +0.1 771,152 + -0.1 771,152 + -0.1 771,152 + -0.1 771,152 + -0.0 771,152 + +0.4 771,153 - -0.2 771,153 - +0.4 771,153 - -0.7 771,153 - +0.6 771,153 - -0.1 771,153 - +0.0 771,153 - +0.4 771,153 - -0.2 771,153 - +0.9 771,153 - +0.4 771,153 - +0.0 771,153 - +0.3 771,153 - +0.5 771,153 - +0.2 771,153 - +0.2 771,198 + -0.4 771,198 + +1.1 771,198 + +0.2 771,198 + -0.2 771,198 + -0.7 771,199 - +0.8 771,199 - -0.2 771,199 - -0.9 771,199 - -0.3 771,199 - -0.1 771,199 - -1.9 771,275 + +0.3 771,275 + -0.9 771,275 + -0.0 771,276 - -0.4 771,276 - +0.8 771,276 - +0.4 771,276 - -0.7 771,276 - -0.4 771,280 + +0.8 771,280 + +1.6 771,280 + -1.4 771,280 + -0.0 771,281 - -1.0 771,314 + -0.8 771,363 + -0.4 771,363 + -0.3 771,363 + -1.8 771,363 + -0.3 771,363 + -0.7 771,364 - -0.5 771,364 - -0.2 771,364 - +0.1 771,517 + ABZR87_RS03710 0.12 +0.5 771,517 + ABZR87_RS03710 0.12 -0.7 771,518 - ABZR87_RS03710 0.12 +0.4 771,541 + ABZR87_RS03710 0.14 -0.8 771,541 + ABZR87_RS03710 0.14 -0.5 771,542 - ABZR87_RS03710 0.14 +0.8 771,554 - ABZR87_RS03710 0.15 +0.3 771,839 - ABZR87_RS03710 0.38 -0.1 771,839 - ABZR87_RS03710 0.38 -0.6 771,865 + ABZR87_RS03710 0.40 -0.1 771,865 + ABZR87_RS03710 0.40 -2.1 771,866 - ABZR87_RS03710 0.40 +0.3 771,921 + ABZR87_RS03710 0.44 -1.1 771,922 - ABZR87_RS03710 0.44 +0.1 771,922 - ABZR87_RS03710 0.44 +0.5 772,064 + ABZR87_RS03710 0.56 -1.1 772,117 + ABZR87_RS03710 0.60 +0.3 772,118 - ABZR87_RS03710 0.60 +0.1 772,156 + ABZR87_RS03710 0.63 -0.0 772,156 + ABZR87_RS03710 0.63 +0.0 772,156 + ABZR87_RS03710 0.63 -0.9 772,157 - ABZR87_RS03710 0.63 -0.6 772,157 - ABZR87_RS03710 0.63 -0.2 772,157 - ABZR87_RS03710 0.63 -0.6 772,157 - ABZR87_RS03710 0.63 -0.2 772,157 - ABZR87_RS03710 0.63 +0.5 772,193 - ABZR87_RS03710 0.66 -0.1 772,193 - ABZR87_RS03710 0.66 -0.7 772,193 - ABZR87_RS03710 0.66 +0.1 772,193 - ABZR87_RS03710 0.66 -0.9 772,193 - ABZR87_RS03710 0.66 +0.1 772,193 - ABZR87_RS03710 0.66 +0.8 772,468 - ABZR87_RS03710 0.88 +0.1 772,695 + -0.3 772,764 + +0.1 772,765 - -0.6 772,765 - -0.6 772,793 - ABZR87_RS03715 0.15 +2.5 772,806 + ABZR87_RS03715 0.17 -0.1 772,807 - ABZR87_RS03715 0.17 -0.6 772,812 + ABZR87_RS03715 0.18 -0.0 772,812 + ABZR87_RS03715 0.18 +0.1 772,812 + ABZR87_RS03715 0.18 -0.8 772,812 + ABZR87_RS03715 0.18 +1.1 772,812 + ABZR87_RS03715 0.18 +0.4 772,812 + ABZR87_RS03715 0.18 +0.2 772,812 + ABZR87_RS03715 0.18 -0.8 772,813 - ABZR87_RS03715 0.18 -1.4 772,813 - ABZR87_RS03715 0.18 -0.5 772,813 - ABZR87_RS03715 0.18 -0.2 772,924 + ABZR87_RS03715 0.38 -0.2 772,924 + ABZR87_RS03715 0.38 -0.6 772,924 + ABZR87_RS03715 0.38 -0.2 772,924 + ABZR87_RS03715 0.38 +0.8 772,925 - ABZR87_RS03715 0.38 +0.6 772,925 - ABZR87_RS03715 0.38 -0.4 772,925 - ABZR87_RS03715 0.38 +0.0 772,925 - ABZR87_RS03715 0.38 -0.2 772,925 - ABZR87_RS03715 0.38 -0.2 772,925 - ABZR87_RS03715 0.38 -0.4 772,941 - ABZR87_RS03715 0.41 -0.2 772,941 - ABZR87_RS03715 0.41 +0.0 773,323 + +0.1 773,323 + -0.5 773,324 - -0.2 773,324 - -0.1 773,324 - -0.5 773,324 - +0.3 773,333 - -0.5 773,333 - +0.5 773,346 + +0.6 773,347 - -1.1 773,355 + +0.1 773,355 + -0.1 773,355 + +0.1 773,355 + -1.2 773,355 + -1.1 773,355 + +0.2 773,355 + -0.5 773,356 - +1.2 773,356 - -0.6 773,356 - +0.8 773,356 - -0.2 773,356 - -0.5 773,358 + -0.0 773,358 + +0.5 773,358 + -1.1 773,358 + -0.4 773,358 + -0.4 773,359 - ABZR87_RS03720 0.10 -0.8 773,359 - ABZR87_RS03720 0.10 -0.7 773,359 - ABZR87_RS03720 0.10 +0.7 773,359 - ABZR87_RS03720 0.10 -0.4 773,359 - ABZR87_RS03720 0.10 -0.5 773,359 - ABZR87_RS03720 0.10 +0.2 773,359 - ABZR87_RS03720 0.10 -0.9 773,359 - ABZR87_RS03720 0.10 +0.5 773,359 - ABZR87_RS03720 0.10 -0.8 773,406 + ABZR87_RS03720 0.23 +0.1 773,406 + ABZR87_RS03720 0.23 +1.0 773,406 + ABZR87_RS03720 0.23 -0.5 773,406 + ABZR87_RS03720 0.23 +0.3 773,407 - ABZR87_RS03720 0.23 -0.9 773,410 + ABZR87_RS03720 0.24 +0.7 773,410 + ABZR87_RS03720 0.24 +1.0 773,411 - ABZR87_RS03720 0.24 -1.0 773,411 - ABZR87_RS03720 0.24 -0.1 773,411 - ABZR87_RS03720 0.24 -0.6 773,411 - ABZR87_RS03720 0.24 +1.5 773,429 + ABZR87_RS03720 0.29 +0.0 773,429 + ABZR87_RS03720 0.29 +0.3 773,441 + ABZR87_RS03720 0.32 -0.7 773,441 + ABZR87_RS03720 0.32 -0.3 773,441 + ABZR87_RS03720 0.32 +0.4 773,578 + ABZR87_RS03720 0.69 -0.4 773,582 + ABZR87_RS03720 0.70 +0.0 773,582 + ABZR87_RS03720 0.70 -0.6
Or see this region's nucleotide sequence