Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS03690 and ABZR87_RS03695 are separated by 159 nucleotides ABZR87_RS03695 and ABZR87_RS03700 are separated by 19 nucleotides ABZR87_RS03700 and ABZR87_RS03705 are separated by 1 nucleotides ABZR87_RS03705 and ABZR87_RS03710 are separated by 313 nucleotides
ABZR87_RS03690: ABZR87_RS03690 - DUF305 domain-containing protein, at 769,255 to 769,770
_RS03690
ABZR87_RS03695: ABZR87_RS03695 - carboxymuconolactone decarboxylase family protein, at 769,930 to 770,262
_RS03695
ABZR87_RS03700: ABZR87_RS03700 - RNA polymerase sigma factor, at 770,282 to 770,839
_RS03700
ABZR87_RS03705: ABZR87_RS03705 - DUF2892 domain-containing protein, at 770,841 to 771,050
_RS03705
ABZR87_RS03710: ABZR87_RS03710 - Hsp70 family protein, at 771,364 to 772,617
_RS03710
Position (kb) 770
771 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 769.292 kb on + strand at 769.292 kb on + strand at 769.293 kb on - strand at 769.411 kb on + strand, within ABZR87_RS03690 at 769.411 kb on + strand, within ABZR87_RS03690 at 769.412 kb on - strand, within ABZR87_RS03690 at 769.425 kb on + strand, within ABZR87_RS03690 at 769.425 kb on + strand, within ABZR87_RS03690 at 769.425 kb on + strand, within ABZR87_RS03690 at 769.425 kb on + strand, within ABZR87_RS03690 at 769.426 kb on - strand, within ABZR87_RS03690 at 769.426 kb on - strand, within ABZR87_RS03690 at 769.487 kb on + strand, within ABZR87_RS03690 at 769.487 kb on + strand, within ABZR87_RS03690 at 769.487 kb on + strand, within ABZR87_RS03690 at 769.487 kb on + strand, within ABZR87_RS03690 at 769.487 kb on + strand, within ABZR87_RS03690 at 769.487 kb on + strand, within ABZR87_RS03690 at 769.487 kb on + strand, within ABZR87_RS03690 at 769.488 kb on - strand, within ABZR87_RS03690 at 769.488 kb on - strand, within ABZR87_RS03690 at 769.488 kb on - strand, within ABZR87_RS03690 at 769.592 kb on + strand, within ABZR87_RS03690 at 769.592 kb on + strand, within ABZR87_RS03690 at 769.592 kb on + strand, within ABZR87_RS03690 at 769.593 kb on - strand, within ABZR87_RS03690 at 769.593 kb on - strand, within ABZR87_RS03690 at 769.593 kb on - strand, within ABZR87_RS03690 at 769.593 kb on - strand, within ABZR87_RS03690 at 769.593 kb on - strand, within ABZR87_RS03690 at 769.593 kb on - strand, within ABZR87_RS03690 at 769.593 kb on - strand, within ABZR87_RS03690 at 769.675 kb on + strand, within ABZR87_RS03690 at 769.675 kb on + strand, within ABZR87_RS03690 at 769.676 kb on - strand, within ABZR87_RS03690 at 769.695 kb on - strand, within ABZR87_RS03690 at 769.695 kb on - strand, within ABZR87_RS03690 at 769.706 kb on + strand, within ABZR87_RS03690 at 769.706 kb on + strand, within ABZR87_RS03690 at 769.707 kb on - strand, within ABZR87_RS03690 at 769.707 kb on - strand, within ABZR87_RS03690 at 769.707 kb on - strand, within ABZR87_RS03690 at 769.763 kb on + strand at 769.763 kb on + strand at 769.764 kb on - strand at 769.764 kb on - strand at 769.764 kb on - strand at 769.764 kb on - strand at 769.764 kb on - strand at 769.764 kb on - strand at 769.852 kb on + strand at 769.853 kb on - strand at 769.853 kb on - strand at 769.853 kb on - strand at 769.853 kb on - strand at 769.931 kb on + strand at 769.931 kb on + strand at 769.931 kb on + strand at 769.931 kb on + strand at 769.931 kb on + strand at 769.931 kb on + strand at 769.931 kb on + strand at 769.931 kb on + strand at 769.931 kb on + strand at 769.931 kb on + strand at 769.931 kb on + strand at 769.931 kb on + strand at 769.931 kb on + strand at 769.932 kb on - strand at 769.932 kb on - strand at 769.932 kb on - strand at 769.932 kb on - strand at 769.932 kb on - strand at 769.932 kb on - strand at 769.932 kb on - strand at 769.932 kb on - strand at 770.034 kb on + strand, within ABZR87_RS03695 at 770.062 kb on - strand, within ABZR87_RS03695 at 770.062 kb on - strand, within ABZR87_RS03695 at 770.062 kb on - strand, within ABZR87_RS03695 at 770.078 kb on + strand, within ABZR87_RS03695 at 770.078 kb on + strand, within ABZR87_RS03695 at 770.078 kb on + strand, within ABZR87_RS03695 at 770.078 kb on + strand, within ABZR87_RS03695 at 770.078 kb on + strand, within ABZR87_RS03695 at 770.079 kb on - strand, within ABZR87_RS03695 at 770.091 kb on + strand, within ABZR87_RS03695 at 770.092 kb on - strand, within ABZR87_RS03695 at 770.092 kb on - strand, within ABZR87_RS03695 at 770.092 kb on - strand, within ABZR87_RS03695 at 770.178 kb on + strand, within ABZR87_RS03695 at 770.249 kb on + strand at 770.249 kb on + strand at 770.250 kb on - strand at 770.250 kb on - strand at 770.284 kb on - strand at 770.320 kb on - strand at 770.399 kb on + strand, within ABZR87_RS03700 at 770.399 kb on + strand, within ABZR87_RS03700 at 770.399 kb on + strand, within ABZR87_RS03700 at 770.399 kb on + strand, within ABZR87_RS03700 at 770.400 kb on - strand, within ABZR87_RS03700 at 770.400 kb on - strand, within ABZR87_RS03700 at 770.400 kb on - strand, within ABZR87_RS03700 at 770.464 kb on - strand, within ABZR87_RS03700 at 770.464 kb on - strand, within ABZR87_RS03700 at 770.464 kb on - strand, within ABZR87_RS03700 at 770.464 kb on - strand, within ABZR87_RS03700 at 770.464 kb on - strand, within ABZR87_RS03700 at 770.464 kb on - strand, within ABZR87_RS03700 at 770.464 kb on - strand, within ABZR87_RS03700 at 770.556 kb on + strand, within ABZR87_RS03700 at 770.556 kb on + strand, within ABZR87_RS03700 at 770.556 kb on + strand, within ABZR87_RS03700 at 770.557 kb on - strand, within ABZR87_RS03700 at 770.584 kb on + strand, within ABZR87_RS03700 at 770.585 kb on - strand, within ABZR87_RS03700 at 770.621 kb on + strand, within ABZR87_RS03700 at 770.622 kb on - strand, within ABZR87_RS03700 at 770.622 kb on - strand, within ABZR87_RS03700 at 770.652 kb on - strand, within ABZR87_RS03700 at 770.671 kb on - strand, within ABZR87_RS03700 at 770.747 kb on + strand, within ABZR87_RS03700 at 770.747 kb on + strand, within ABZR87_RS03700 at 770.795 kb on - strand at 770.815 kb on + strand at 770.815 kb on + strand at 770.815 kb on + strand at 770.815 kb on + strand at 770.815 kb on + strand at 770.815 kb on + strand at 770.815 kb on + strand at 770.816 kb on - strand at 770.816 kb on - strand at 770.816 kb on - strand at 770.816 kb on - strand at 770.816 kb on - strand at 770.850 kb on + strand at 770.851 kb on - strand at 770.972 kb on + strand, within ABZR87_RS03705 at 770.973 kb on - strand, within ABZR87_RS03705 at 771.043 kb on + strand at 771.043 kb on + strand at 771.043 kb on + strand at 771.043 kb on + strand at 771.044 kb on - strand at 771.044 kb on - strand at 771.044 kb on - strand at 771.050 kb on + strand at 771.050 kb on + strand at 771.050 kb on + strand at 771.050 kb on + strand at 771.050 kb on + strand at 771.050 kb on + strand at 771.050 kb on + strand at 771.050 kb on + strand at 771.050 kb on + strand at 771.050 kb on + strand at 771.051 kb on - strand at 771.051 kb on - strand at 771.051 kb on - strand at 771.051 kb on - strand at 771.051 kb on - strand at 771.051 kb on - strand at 771.051 kb on - strand at 771.051 kb on - strand at 771.051 kb on - strand at 771.051 kb on - strand at 771.088 kb on + strand at 771.088 kb on + strand at 771.088 kb on + strand at 771.088 kb on + strand at 771.089 kb on - strand at 771.089 kb on - strand at 771.089 kb on - strand at 771.089 kb on - strand at 771.089 kb on - strand at 771.089 kb on - strand at 771.125 kb on + strand at 771.129 kb on + strand at 771.130 kb on - strand at 771.130 kb on - strand at 771.130 kb on - strand at 771.130 kb on - strand at 771.130 kb on - strand at 771.152 kb on + strand at 771.152 kb on + strand at 771.152 kb on + strand at 771.152 kb on + strand at 771.152 kb on + strand at 771.152 kb on + strand at 771.152 kb on + strand at 771.152 kb on + strand at 771.152 kb on + strand at 771.152 kb on + strand at 771.152 kb on + strand at 771.153 kb on - strand at 771.153 kb on - strand at 771.153 kb on - strand at 771.153 kb on - strand at 771.153 kb on - strand at 771.153 kb on - strand at 771.153 kb on - strand at 771.153 kb on - strand at 771.153 kb on - strand at 771.153 kb on - strand at 771.153 kb on - strand at 771.153 kb on - strand at 771.153 kb on - strand at 771.153 kb on - strand at 771.153 kb on - strand at 771.198 kb on + strand at 771.198 kb on + strand at 771.198 kb on + strand at 771.198 kb on + strand at 771.198 kb on + strand at 771.199 kb on - strand at 771.199 kb on - strand at 771.199 kb on - strand at 771.199 kb on - strand at 771.199 kb on - strand at 771.199 kb on - strand at 771.275 kb on + strand at 771.275 kb on + strand at 771.275 kb on + strand at 771.276 kb on - strand at 771.276 kb on - strand at 771.276 kb on - strand at 771.276 kb on - strand at 771.276 kb on - strand at 771.280 kb on + strand at 771.280 kb on + strand at 771.280 kb on + strand at 771.280 kb on + strand at 771.281 kb on - strand at 771.314 kb on + strand at 771.363 kb on + strand at 771.363 kb on + strand at 771.363 kb on + strand at 771.363 kb on + strand at 771.363 kb on + strand at 771.364 kb on - strand at 771.364 kb on - strand at 771.364 kb on - strand at 771.517 kb on + strand, within ABZR87_RS03710 at 771.517 kb on + strand, within ABZR87_RS03710 at 771.518 kb on - strand, within ABZR87_RS03710 at 771.541 kb on + strand, within ABZR87_RS03710 at 771.541 kb on + strand, within ABZR87_RS03710 at 771.542 kb on - strand, within ABZR87_RS03710 at 771.554 kb on - strand, within ABZR87_RS03710 at 771.839 kb on - strand, within ABZR87_RS03710 at 771.839 kb on - strand, within ABZR87_RS03710
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 769,292 + -0.3 769,292 + +1.7 769,293 - +0.0 769,411 + ABZR87_RS03690 0.30 -0.3 769,411 + ABZR87_RS03690 0.30 +0.4 769,412 - ABZR87_RS03690 0.30 -0.4 769,425 + ABZR87_RS03690 0.33 +0.4 769,425 + ABZR87_RS03690 0.33 -0.2 769,425 + ABZR87_RS03690 0.33 -1.1 769,425 + ABZR87_RS03690 0.33 -0.8 769,426 - ABZR87_RS03690 0.33 -0.6 769,426 - ABZR87_RS03690 0.33 +0.3 769,487 + ABZR87_RS03690 0.45 -0.2 769,487 + ABZR87_RS03690 0.45 -0.2 769,487 + ABZR87_RS03690 0.45 +0.4 769,487 + ABZR87_RS03690 0.45 +0.0 769,487 + ABZR87_RS03690 0.45 +0.9 769,487 + ABZR87_RS03690 0.45 +0.7 769,487 + ABZR87_RS03690 0.45 +0.3 769,488 - ABZR87_RS03690 0.45 -1.7 769,488 - ABZR87_RS03690 0.45 -1.4 769,488 - ABZR87_RS03690 0.45 -0.3 769,592 + ABZR87_RS03690 0.65 -0.7 769,592 + ABZR87_RS03690 0.65 -0.1 769,592 + ABZR87_RS03690 0.65 -0.3 769,593 - ABZR87_RS03690 0.66 +0.2 769,593 - ABZR87_RS03690 0.66 +0.2 769,593 - ABZR87_RS03690 0.66 +0.7 769,593 - ABZR87_RS03690 0.66 -1.4 769,593 - ABZR87_RS03690 0.66 -2.7 769,593 - ABZR87_RS03690 0.66 -0.6 769,593 - ABZR87_RS03690 0.66 -0.1 769,675 + ABZR87_RS03690 0.81 -0.5 769,675 + ABZR87_RS03690 0.81 -0.6 769,676 - ABZR87_RS03690 0.82 -0.4 769,695 - ABZR87_RS03690 0.85 -1.1 769,695 - ABZR87_RS03690 0.85 -0.1 769,706 + ABZR87_RS03690 0.87 +0.0 769,706 + ABZR87_RS03690 0.87 -0.6 769,707 - ABZR87_RS03690 0.88 +0.1 769,707 - ABZR87_RS03690 0.88 +0.9 769,707 - ABZR87_RS03690 0.88 +0.1 769,763 + -1.3 769,763 + -0.0 769,764 - +0.1 769,764 - -1.5 769,764 - -0.7 769,764 - -0.1 769,764 - -0.8 769,764 - +0.4 769,852 + +0.2 769,853 - -0.2 769,853 - +0.7 769,853 - +0.3 769,853 - +0.2 769,931 + -0.7 769,931 + +0.1 769,931 + +1.2 769,931 + +0.4 769,931 + +0.9 769,931 + +1.5 769,931 + +1.1 769,931 + -0.7 769,931 + +2.4 769,931 + +0.2 769,931 + +0.7 769,931 + -0.5 769,931 + -1.1 769,932 - -0.4 769,932 - -0.7 769,932 - +0.2 769,932 - -0.4 769,932 - -0.5 769,932 - +1.0 769,932 - -1.2 769,932 - -0.3 770,034 + ABZR87_RS03695 0.31 -0.0 770,062 - ABZR87_RS03695 0.40 -0.7 770,062 - ABZR87_RS03695 0.40 -0.2 770,062 - ABZR87_RS03695 0.40 -0.7 770,078 + ABZR87_RS03695 0.44 +0.4 770,078 + ABZR87_RS03695 0.44 +0.8 770,078 + ABZR87_RS03695 0.44 +1.7 770,078 + ABZR87_RS03695 0.44 +0.3 770,078 + ABZR87_RS03695 0.44 -0.3 770,079 - ABZR87_RS03695 0.45 +2.7 770,091 + ABZR87_RS03695 0.48 +1.7 770,092 - ABZR87_RS03695 0.49 -0.4 770,092 - ABZR87_RS03695 0.49 +0.1 770,092 - ABZR87_RS03695 0.49 +0.2 770,178 + ABZR87_RS03695 0.74 +0.7 770,249 + +0.7 770,249 + -0.1 770,250 - +0.0 770,250 - -0.1 770,284 - +0.2 770,320 - +0.2 770,399 + ABZR87_RS03700 0.21 +0.4 770,399 + ABZR87_RS03700 0.21 -0.5 770,399 + ABZR87_RS03700 0.21 +0.4 770,399 + ABZR87_RS03700 0.21 +1.2 770,400 - ABZR87_RS03700 0.21 -0.5 770,400 - ABZR87_RS03700 0.21 -0.3 770,400 - ABZR87_RS03700 0.21 -1.8 770,464 - ABZR87_RS03700 0.33 -1.9 770,464 - ABZR87_RS03700 0.33 +0.3 770,464 - ABZR87_RS03700 0.33 -1.1 770,464 - ABZR87_RS03700 0.33 +0.2 770,464 - ABZR87_RS03700 0.33 +1.2 770,464 - ABZR87_RS03700 0.33 -1.9 770,464 - ABZR87_RS03700 0.33 -1.3 770,556 + ABZR87_RS03700 0.49 +0.3 770,556 + ABZR87_RS03700 0.49 +0.1 770,556 + ABZR87_RS03700 0.49 -1.2 770,557 - ABZR87_RS03700 0.49 -0.2 770,584 + ABZR87_RS03700 0.54 +3.3 770,585 - ABZR87_RS03700 0.54 -0.7 770,621 + ABZR87_RS03700 0.61 +0.8 770,622 - ABZR87_RS03700 0.61 +0.7 770,622 - ABZR87_RS03700 0.61 +0.2 770,652 - ABZR87_RS03700 0.66 +0.3 770,671 - ABZR87_RS03700 0.70 +0.1 770,747 + ABZR87_RS03700 0.83 -0.7 770,747 + ABZR87_RS03700 0.83 -0.3 770,795 - -0.5 770,815 + +0.6 770,815 + -0.0 770,815 + -0.8 770,815 + +4.1 770,815 + -0.9 770,815 + +1.0 770,815 + +0.5 770,816 - -0.4 770,816 - +0.4 770,816 - -0.6 770,816 - +0.6 770,816 - +0.2 770,850 + +1.7 770,851 - +0.7 770,972 + ABZR87_RS03705 0.62 +1.6 770,973 - ABZR87_RS03705 0.63 -0.6 771,043 + +0.2 771,043 + -0.4 771,043 + -1.2 771,043 + -0.1 771,044 - +0.3 771,044 - +0.2 771,044 - +1.1 771,050 + +0.4 771,050 + -0.2 771,050 + +0.1 771,050 + +0.5 771,050 + +0.1 771,050 + -0.3 771,050 + -1.5 771,050 + -0.6 771,050 + -1.1 771,050 + +0.4 771,051 - -0.2 771,051 - -0.0 771,051 - +0.3 771,051 - -0.2 771,051 - +0.4 771,051 - +0.9 771,051 - -0.3 771,051 - -0.3 771,051 - +0.9 771,051 - -0.2 771,088 + +0.7 771,088 + -0.6 771,088 + -2.0 771,088 + -0.4 771,089 - -2.0 771,089 - +0.2 771,089 - -0.1 771,089 - -1.8 771,089 - +1.2 771,089 - -0.1 771,125 + +1.4 771,129 + -0.3 771,130 - -1.5 771,130 - +0.9 771,130 - -0.2 771,130 - -0.3 771,130 - -1.3 771,152 + -0.7 771,152 + +0.7 771,152 + -0.2 771,152 + -0.3 771,152 + +1.7 771,152 + +0.1 771,152 + -0.1 771,152 + -0.1 771,152 + -0.1 771,152 + -0.0 771,152 + +0.4 771,153 - -0.2 771,153 - +0.4 771,153 - -0.7 771,153 - +0.6 771,153 - -0.1 771,153 - +0.0 771,153 - +0.4 771,153 - -0.2 771,153 - +0.9 771,153 - +0.4 771,153 - +0.0 771,153 - +0.3 771,153 - +0.5 771,153 - +0.2 771,153 - +0.2 771,198 + -0.4 771,198 + +1.1 771,198 + +0.2 771,198 + -0.2 771,198 + -0.7 771,199 - +0.8 771,199 - -0.2 771,199 - -0.9 771,199 - -0.3 771,199 - -0.1 771,199 - -1.9 771,275 + +0.3 771,275 + -0.9 771,275 + -0.0 771,276 - -0.4 771,276 - +0.8 771,276 - +0.4 771,276 - -0.7 771,276 - -0.4 771,280 + +0.8 771,280 + +1.6 771,280 + -1.4 771,280 + -0.0 771,281 - -1.0 771,314 + -0.8 771,363 + -0.4 771,363 + -0.3 771,363 + -1.8 771,363 + -0.3 771,363 + -0.7 771,364 - -0.5 771,364 - -0.2 771,364 - +0.1 771,517 + ABZR87_RS03710 0.12 +0.5 771,517 + ABZR87_RS03710 0.12 -0.7 771,518 - ABZR87_RS03710 0.12 +0.4 771,541 + ABZR87_RS03710 0.14 -0.8 771,541 + ABZR87_RS03710 0.14 -0.5 771,542 - ABZR87_RS03710 0.14 +0.8 771,554 - ABZR87_RS03710 0.15 +0.3 771,839 - ABZR87_RS03710 0.38 -0.1 771,839 - ABZR87_RS03710 0.38 -0.6
Or see this region's nucleotide sequence