Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS02470 and ABZR87_RS02475 are separated by 47 nucleotides ABZR87_RS02475 and ABZR87_RS02480 are separated by 75 nucleotides ABZR87_RS02480 and ABZR87_RS02485 are separated by 8 nucleotides ABZR87_RS02485 and ABZR87_RS02490 are separated by 55 nucleotides
ABZR87_RS02470: ABZR87_RS02470 - imidazole glycerol phosphate synthase subunit HisF, at 525,179 to 525,955
_RS02470
ABZR87_RS02475: ABZR87_RS02475 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 526,003 to 526,746
_RS02475
ABZR87_RS02480: ABZR87_RS02480 - imidazole glycerol phosphate synthase subunit HisH, at 526,822 to 527,475
_RS02480
ABZR87_RS02485: ABZR87_RS02485 - MarC family protein, at 527,484 to 528,107
_RS02485
ABZR87_RS02490: ABZR87_RS02490 - imidazoleglycerol-phosphate dehydratase HisB, at 528,163 to 528,753
_RS02490
Position (kb) 526
527
528 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 525.950 kb on + strand at 525.950 kb on + strand at 525.950 kb on + strand at 526.746 kb on + strand at 526.747 kb on - strand at 526.747 kb on - strand at 526.747 kb on - strand at 526.747 kb on - strand at 526.747 kb on - strand at 526.906 kb on - strand, within ABZR87_RS02480 at 526.989 kb on - strand, within ABZR87_RS02480 at 526.995 kb on + strand, within ABZR87_RS02480 at 526.996 kb on - strand, within ABZR87_RS02480 at 526.996 kb on - strand, within ABZR87_RS02480 at 526.996 kb on - strand, within ABZR87_RS02480 at 526.996 kb on - strand, within ABZR87_RS02480 at 526.996 kb on - strand, within ABZR87_RS02480 at 527.007 kb on - strand, within ABZR87_RS02480 at 527.007 kb on - strand, within ABZR87_RS02480 at 527.012 kb on + strand, within ABZR87_RS02480 at 527.013 kb on - strand, within ABZR87_RS02480 at 527.013 kb on - strand, within ABZR87_RS02480 at 527.013 kb on - strand, within ABZR87_RS02480 at 527.013 kb on - strand, within ABZR87_RS02480 at 527.175 kb on - strand, within ABZR87_RS02480 at 527.470 kb on - strand at 527.470 kb on - strand at 527.480 kb on + strand at 527.481 kb on - strand at 527.481 kb on - strand at 527.481 kb on - strand at 527.481 kb on - strand at 527.481 kb on - strand at 527.481 kb on - strand at 527.481 kb on - strand at 527.481 kb on - strand at 527.485 kb on + strand at 527.486 kb on - strand at 527.486 kb on - strand at 527.486 kb on - strand at 527.486 kb on - strand at 527.486 kb on - strand at 527.497 kb on - strand at 527.905 kb on - strand, within ABZR87_RS02485 at 528.165 kb on - strand at 528.165 kb on - strand at 528.165 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 525,950 + -1.0 525,950 + -0.2 525,950 + +0.6 526,746 + -1.0 526,747 - -2.2 526,747 - +0.2 526,747 - +0.0 526,747 - +0.4 526,747 - +0.9 526,906 - ABZR87_RS02480 0.13 +2.8 526,989 - ABZR87_RS02480 0.26 -0.2 526,995 + ABZR87_RS02480 0.26 -1.5 526,996 - ABZR87_RS02480 0.27 +0.2 526,996 - ABZR87_RS02480 0.27 +1.0 526,996 - ABZR87_RS02480 0.27 -1.4 526,996 - ABZR87_RS02480 0.27 -2.0 526,996 - ABZR87_RS02480 0.27 -1.0 527,007 - ABZR87_RS02480 0.28 -2.1 527,007 - ABZR87_RS02480 0.28 -0.2 527,012 + ABZR87_RS02480 0.29 +1.8 527,013 - ABZR87_RS02480 0.29 -2.3 527,013 - ABZR87_RS02480 0.29 +1.7 527,013 - ABZR87_RS02480 0.29 -0.8 527,013 - ABZR87_RS02480 0.29 -0.6 527,175 - ABZR87_RS02480 0.54 -1.0 527,470 - -0.1 527,470 - -0.6 527,480 + -0.6 527,481 - +0.4 527,481 - +0.2 527,481 - +0.0 527,481 - -1.2 527,481 - +0.2 527,481 - -0.4 527,481 - -1.1 527,481 - +0.2 527,485 + +0.6 527,486 - -1.4 527,486 - -0.3 527,486 - -0.8 527,486 - +0.5 527,486 - +0.4 527,497 - -0.7 527,905 - ABZR87_RS02485 0.67 +0.1 528,165 - +0.8 528,165 - -0.4 528,165 - -0.0
Or see this region's nucleotide sequence