Strain Fitness in Ralstonia sp. UNC404CL21Col around ABZR87_RS02395

Experiment: Acanthamoeba sp., muxed culture-2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABZR87_RS02385 and ABZR87_RS02390 are separated by 245 nucleotidesABZR87_RS02390 and ABZR87_RS02395 are separated by 12 nucleotidesABZR87_RS02395 and ABZR87_RS02400 are separated by 19 nucleotides ABZR87_RS02385: ABZR87_RS02385 - ubiquinol-cytochrome c reductase iron-sulfur subunit, at 509,375 to 509,983 _RS02385 ABZR87_RS02390: ABZR87_RS02390 - large conductance mechanosensitive channel protein MscL, at 510,229 to 510,657 _RS02390 ABZR87_RS02395: ABZR87_RS02395 - Nif3-like dinuclear metal center hexameric protein, at 510,670 to 511,416 _RS02395 ABZR87_RS02400: ABZR87_RS02400 - Do family serine endopeptidase, at 511,436 to 512,650 _RS02400 Position (kb) 510 511 512Strain fitness (log2 ratio) -2 -1 0 1 2at 509.835 kb on - strand, within ABZR87_RS02385at 509.835 kb on - strand, within ABZR87_RS02385at 509.942 kb on + strandat 509.942 kb on + strandat 509.942 kb on + strandat 509.943 kb on - strandat 509.943 kb on - strandat 509.943 kb on - strandat 509.943 kb on - strandat 509.943 kb on - strandat 510.011 kb on + strandat 510.011 kb on + strandat 510.012 kb on - strandat 510.046 kb on - strandat 510.046 kb on - strandat 510.088 kb on + strandat 510.090 kb on + strandat 510.090 kb on + strandat 510.090 kb on + strandat 510.091 kb on - strandat 510.091 kb on - strandat 510.091 kb on - strandat 510.091 kb on - strandat 510.185 kb on + strandat 510.369 kb on + strand, within ABZR87_RS02390at 510.369 kb on + strand, within ABZR87_RS02390at 510.369 kb on + strand, within ABZR87_RS02390at 510.369 kb on + strand, within ABZR87_RS02390at 510.369 kb on + strand, within ABZR87_RS02390at 510.370 kb on - strand, within ABZR87_RS02390at 510.370 kb on - strand, within ABZR87_RS02390at 510.370 kb on - strand, within ABZR87_RS02390at 510.370 kb on - strand, within ABZR87_RS02390at 510.370 kb on - strand, within ABZR87_RS02390at 510.370 kb on - strand, within ABZR87_RS02390at 510.370 kb on - strand, within ABZR87_RS02390at 510.370 kb on - strand, within ABZR87_RS02390at 510.438 kb on + strand, within ABZR87_RS02390at 510.438 kb on + strand, within ABZR87_RS02390at 510.438 kb on + strand, within ABZR87_RS02390at 510.438 kb on + strand, within ABZR87_RS02390at 510.438 kb on + strand, within ABZR87_RS02390at 510.438 kb on + strand, within ABZR87_RS02390at 510.438 kb on + strand, within ABZR87_RS02390at 510.438 kb on + strand, within ABZR87_RS02390at 510.439 kb on - strand, within ABZR87_RS02390at 510.439 kb on - strand, within ABZR87_RS02390at 510.439 kb on - strand, within ABZR87_RS02390at 510.439 kb on - strand, within ABZR87_RS02390at 510.439 kb on - strand, within ABZR87_RS02390at 510.439 kb on - strand, within ABZR87_RS02390at 510.439 kb on - strand, within ABZR87_RS02390at 510.439 kb on - strand, within ABZR87_RS02390at 510.528 kb on + strand, within ABZR87_RS02390at 510.529 kb on - strand, within ABZR87_RS02390at 510.655 kb on + strandat 510.656 kb on - strandat 510.656 kb on - strandat 510.656 kb on - strandat 510.706 kb on + strandat 510.706 kb on + strandat 510.706 kb on + strandat 510.706 kb on + strandat 510.706 kb on + strandat 510.707 kb on - strandat 510.707 kb on - strandat 510.707 kb on - strandat 510.707 kb on - strandat 510.785 kb on + strand, within ABZR87_RS02395at 510.785 kb on + strand, within ABZR87_RS02395at 510.785 kb on + strand, within ABZR87_RS02395at 510.785 kb on + strand, within ABZR87_RS02395at 510.785 kb on + strand, within ABZR87_RS02395at 510.785 kb on + strand, within ABZR87_RS02395at 510.786 kb on - strand, within ABZR87_RS02395at 510.786 kb on - strand, within ABZR87_RS02395at 510.786 kb on - strand, within ABZR87_RS02395at 510.786 kb on - strand, within ABZR87_RS02395at 510.786 kb on - strand, within ABZR87_RS02395at 510.786 kb on - strand, within ABZR87_RS02395at 510.786 kb on - strand, within ABZR87_RS02395at 510.845 kb on + strand, within ABZR87_RS02395at 510.845 kb on + strand, within ABZR87_RS02395at 510.845 kb on + strand, within ABZR87_RS02395at 510.846 kb on - strand, within ABZR87_RS02395at 510.846 kb on - strand, within ABZR87_RS02395at 510.846 kb on - strand, within ABZR87_RS02395at 510.846 kb on - strand, within ABZR87_RS02395at 510.846 kb on - strand, within ABZR87_RS02395at 510.846 kb on - strand, within ABZR87_RS02395at 510.846 kb on - strand, within ABZR87_RS02395at 510.846 kb on - strand, within ABZR87_RS02395at 510.846 kb on - strand, within ABZR87_RS02395at 510.846 kb on - strand, within ABZR87_RS02395at 510.995 kb on - strand, within ABZR87_RS02395at 510.995 kb on - strand, within ABZR87_RS02395at 510.995 kb on - strand, within ABZR87_RS02395at 511.131 kb on - strand, within ABZR87_RS02395at 511.220 kb on + strand, within ABZR87_RS02395at 511.220 kb on + strand, within ABZR87_RS02395at 511.220 kb on + strand, within ABZR87_RS02395at 511.220 kb on + strand, within ABZR87_RS02395at 511.221 kb on - strand, within ABZR87_RS02395at 511.221 kb on - strand, within ABZR87_RS02395at 511.221 kb on - strand, within ABZR87_RS02395at 511.221 kb on - strand, within ABZR87_RS02395at 511.311 kb on + strand, within ABZR87_RS02395at 511.312 kb on - strand, within ABZR87_RS02395at 511.312 kb on - strand, within ABZR87_RS02395at 511.336 kb on + strand, within ABZR87_RS02395at 511.336 kb on + strand, within ABZR87_RS02395at 511.337 kb on - strand, within ABZR87_RS02395at 511.337 kb on - strand, within ABZR87_RS02395at 511.337 kb on - strand, within ABZR87_RS02395at 511.352 kb on + strandat 511.352 kb on + strandat 511.352 kb on + strandat 511.352 kb on + strandat 511.353 kb on - strandat 511.353 kb on - strandat 511.353 kb on - strandat 511.353 kb on - strandat 511.391 kb on + strandat 511.391 kb on + strandat 511.435 kb on + strandat 511.435 kb on + strandat 511.435 kb on + strandat 511.436 kb on - strandat 511.436 kb on - strandat 511.436 kb on - strandat 511.436 kb on - strandat 511.436 kb on - strandat 511.622 kb on + strand, within ABZR87_RS02400at 511.623 kb on - strand, within ABZR87_RS02400at 511.623 kb on - strand, within ABZR87_RS02400at 511.824 kb on + strand, within ABZR87_RS02400at 511.824 kb on + strand, within ABZR87_RS02400at 511.824 kb on + strand, within ABZR87_RS02400at 511.824 kb on + strand, within ABZR87_RS02400at 511.825 kb on - strand, within ABZR87_RS02400at 511.825 kb on - strand, within ABZR87_RS02400at 511.969 kb on + strand, within ABZR87_RS02400at 511.970 kb on - strand, within ABZR87_RS02400at 511.970 kb on - strand, within ABZR87_RS02400at 511.970 kb on - strand, within ABZR87_RS02400at 512.204 kb on + strand, within ABZR87_RS02400at 512.204 kb on + strand, within ABZR87_RS02400at 512.204 kb on + strand, within ABZR87_RS02400at 512.204 kb on + strand, within ABZR87_RS02400at 512.204 kb on + strand, within ABZR87_RS02400at 512.281 kb on + strand, within ABZR87_RS02400at 512.281 kb on + strand, within ABZR87_RS02400at 512.281 kb on + strand, within ABZR87_RS02400at 512.281 kb on + strand, within ABZR87_RS02400at 512.281 kb on + strand, within ABZR87_RS02400at 512.282 kb on - strand, within ABZR87_RS02400

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Per-strain Table

Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2
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509,835 - ABZR87_RS02385 0.76 -0.9
509,835 - ABZR87_RS02385 0.76 +0.8
509,942 + -1.8
509,942 + -0.2
509,942 + +0.1
509,943 - -0.8
509,943 - +0.0
509,943 - +1.1
509,943 - +1.3
509,943 - +0.7
510,011 + -1.5
510,011 + -0.4
510,012 - -0.8
510,046 - -0.1
510,046 - -1.9
510,088 + +1.0
510,090 + -1.7
510,090 + +0.5
510,090 + +0.6
510,091 - +1.3
510,091 - +1.2
510,091 - -0.1
510,091 - +0.4
510,185 + -1.3
510,369 + ABZR87_RS02390 0.33 +0.0
510,369 + ABZR87_RS02390 0.33 -0.7
510,369 + ABZR87_RS02390 0.33 +0.3
510,369 + ABZR87_RS02390 0.33 +0.1
510,369 + ABZR87_RS02390 0.33 -0.1
510,370 - ABZR87_RS02390 0.33 +0.2
510,370 - ABZR87_RS02390 0.33 -0.1
510,370 - ABZR87_RS02390 0.33 +0.1
510,370 - ABZR87_RS02390 0.33 +0.2
510,370 - ABZR87_RS02390 0.33 +0.8
510,370 - ABZR87_RS02390 0.33 -0.1
510,370 - ABZR87_RS02390 0.33 -0.1
510,370 - ABZR87_RS02390 0.33 +0.0
510,438 + ABZR87_RS02390 0.49 -0.1
510,438 + ABZR87_RS02390 0.49 -0.1
510,438 + ABZR87_RS02390 0.49 +0.2
510,438 + ABZR87_RS02390 0.49 -0.2
510,438 + ABZR87_RS02390 0.49 +0.1
510,438 + ABZR87_RS02390 0.49 -0.3
510,438 + ABZR87_RS02390 0.49 -0.8
510,438 + ABZR87_RS02390 0.49 -0.2
510,439 - ABZR87_RS02390 0.49 -0.0
510,439 - ABZR87_RS02390 0.49 +0.1
510,439 - ABZR87_RS02390 0.49 -0.5
510,439 - ABZR87_RS02390 0.49 -0.1
510,439 - ABZR87_RS02390 0.49 +0.4
510,439 - ABZR87_RS02390 0.49 -0.2
510,439 - ABZR87_RS02390 0.49 -0.5
510,439 - ABZR87_RS02390 0.49 -0.1
510,528 + ABZR87_RS02390 0.70 +0.2
510,529 - ABZR87_RS02390 0.70 -0.0
510,655 + -0.2
510,656 - -0.2
510,656 - +0.2
510,656 - -1.2
510,706 + +0.2
510,706 + +0.1
510,706 + +0.3
510,706 + -1.1
510,706 + +0.5
510,707 - -0.4
510,707 - -0.2
510,707 - +1.1
510,707 - +0.0
510,785 + ABZR87_RS02395 0.15 +0.4
510,785 + ABZR87_RS02395 0.15 +0.1
510,785 + ABZR87_RS02395 0.15 -0.2
510,785 + ABZR87_RS02395 0.15 +0.5
510,785 + ABZR87_RS02395 0.15 +0.4
510,785 + ABZR87_RS02395 0.15 +0.2
510,786 - ABZR87_RS02395 0.16 +0.5
510,786 - ABZR87_RS02395 0.16 -0.5
510,786 - ABZR87_RS02395 0.16 -1.0
510,786 - ABZR87_RS02395 0.16 +0.1
510,786 - ABZR87_RS02395 0.16 -0.8
510,786 - ABZR87_RS02395 0.16 -1.3
510,786 - ABZR87_RS02395 0.16 -0.3
510,845 + ABZR87_RS02395 0.23 +0.8
510,845 + ABZR87_RS02395 0.23 -1.0
510,845 + ABZR87_RS02395 0.23 -0.2
510,846 - ABZR87_RS02395 0.24 -0.2
510,846 - ABZR87_RS02395 0.24 +0.4
510,846 - ABZR87_RS02395 0.24 -0.0
510,846 - ABZR87_RS02395 0.24 +0.1
510,846 - ABZR87_RS02395 0.24 -0.2
510,846 - ABZR87_RS02395 0.24 -0.1
510,846 - ABZR87_RS02395 0.24 +0.4
510,846 - ABZR87_RS02395 0.24 +0.6
510,846 - ABZR87_RS02395 0.24 +0.9
510,846 - ABZR87_RS02395 0.24 -0.5
510,995 - ABZR87_RS02395 0.44 -0.6
510,995 - ABZR87_RS02395 0.44 -1.0
510,995 - ABZR87_RS02395 0.44 -0.6
511,131 - ABZR87_RS02395 0.62 +0.2
511,220 + ABZR87_RS02395 0.74 +0.6
511,220 + ABZR87_RS02395 0.74 +0.2
511,220 + ABZR87_RS02395 0.74 -2.2
511,220 + ABZR87_RS02395 0.74 -0.3
511,221 - ABZR87_RS02395 0.74 +0.8
511,221 - ABZR87_RS02395 0.74 -0.8
511,221 - ABZR87_RS02395 0.74 +1.0
511,221 - ABZR87_RS02395 0.74 -0.8
511,311 + ABZR87_RS02395 0.86 -1.2
511,312 - ABZR87_RS02395 0.86 +0.0
511,312 - ABZR87_RS02395 0.86 +0.4
511,336 + ABZR87_RS02395 0.89 -0.8
511,336 + ABZR87_RS02395 0.89 -0.4
511,337 - ABZR87_RS02395 0.89 -0.2
511,337 - ABZR87_RS02395 0.89 -0.8
511,337 - ABZR87_RS02395 0.89 +0.8
511,352 + +0.0
511,352 + -0.3
511,352 + -0.9
511,352 + -0.3
511,353 - +0.3
511,353 - +0.8
511,353 - -0.1
511,353 - +0.8
511,391 + +1.6
511,391 + -2.5
511,435 + +0.7
511,435 + +0.5
511,435 + +1.0
511,436 - +1.3
511,436 - +1.5
511,436 - +1.4
511,436 - +0.4
511,436 - +1.1
511,622 + ABZR87_RS02400 0.15 +1.1
511,623 - ABZR87_RS02400 0.15 +1.1
511,623 - ABZR87_RS02400 0.15 +1.0
511,824 + ABZR87_RS02400 0.32 +1.5
511,824 + ABZR87_RS02400 0.32 +0.9
511,824 + ABZR87_RS02400 0.32 +1.4
511,824 + ABZR87_RS02400 0.32 +0.9
511,825 - ABZR87_RS02400 0.32 +1.3
511,825 - ABZR87_RS02400 0.32 +1.1
511,969 + ABZR87_RS02400 0.44 +1.7
511,970 - ABZR87_RS02400 0.44 +1.4
511,970 - ABZR87_RS02400 0.44 +1.8
511,970 - ABZR87_RS02400 0.44 +1.0
512,204 + ABZR87_RS02400 0.63 +0.9
512,204 + ABZR87_RS02400 0.63 +1.2
512,204 + ABZR87_RS02400 0.63 +1.3
512,204 + ABZR87_RS02400 0.63 +1.5
512,204 + ABZR87_RS02400 0.63 +1.0
512,281 + ABZR87_RS02400 0.70 +1.4
512,281 + ABZR87_RS02400 0.70 +1.6
512,281 + ABZR87_RS02400 0.70 +1.2
512,281 + ABZR87_RS02400 0.70 +1.1
512,281 + ABZR87_RS02400 0.70 +1.3
512,282 - ABZR87_RS02400 0.70 +1.5

Or see this region's nucleotide sequence