Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS02335 and ABZR87_RS02340 are separated by 635 nucleotides ABZR87_RS02340 and ABZR87_RS02345 are separated by 31 nucleotides ABZR87_RS02345 and ABZR87_RS02350 are separated by 224 nucleotides
ABZR87_RS02335: ABZR87_RS02335 - ubiquinol oxidase subunit II, at 499,794 to 500,747
_RS02335
ABZR87_RS02340: ABZR87_RS02340 - Nramp family divalent metal transporter, at 501,383 to 503,026
_RS02340
ABZR87_RS02345: ABZR87_RS02345 - hypothetical protein, at 503,058 to 503,264
_RS02345
ABZR87_RS02350: ABZR87_RS02350 - carbohydrate porin, at 503,489 to 504,883
_RS02350
Position (kb) 501
502
503
504 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 500.425 kb on + strand, within ABZR87_RS02335 at 500.426 kb on - strand, within ABZR87_RS02335 at 500.450 kb on + strand, within ABZR87_RS02335 at 500.450 kb on + strand, within ABZR87_RS02335 at 500.450 kb on + strand, within ABZR87_RS02335 at 500.451 kb on - strand, within ABZR87_RS02335 at 500.452 kb on + strand, within ABZR87_RS02335 at 500.452 kb on + strand, within ABZR87_RS02335 at 500.452 kb on + strand, within ABZR87_RS02335 at 500.452 kb on + strand, within ABZR87_RS02335 at 500.452 kb on + strand, within ABZR87_RS02335 at 500.452 kb on + strand, within ABZR87_RS02335 at 500.453 kb on - strand, within ABZR87_RS02335 at 500.453 kb on - strand, within ABZR87_RS02335 at 500.453 kb on - strand, within ABZR87_RS02335 at 500.453 kb on - strand, within ABZR87_RS02335 at 500.453 kb on - strand, within ABZR87_RS02335 at 500.453 kb on - strand, within ABZR87_RS02335 at 500.543 kb on - strand, within ABZR87_RS02335 at 500.569 kb on + strand, within ABZR87_RS02335 at 500.569 kb on + strand, within ABZR87_RS02335 at 500.747 kb on + strand at 500.747 kb on + strand at 500.747 kb on + strand at 500.747 kb on + strand at 500.747 kb on + strand at 500.747 kb on + strand at 500.747 kb on + strand at 500.747 kb on + strand at 500.747 kb on + strand at 500.747 kb on + strand at 500.748 kb on - strand at 500.748 kb on - strand at 500.748 kb on - strand at 500.748 kb on - strand at 500.748 kb on - strand at 500.748 kb on - strand at 500.748 kb on - strand at 500.877 kb on - strand at 500.877 kb on - strand at 500.892 kb on + strand at 500.892 kb on + strand at 501.076 kb on + strand at 501.077 kb on - strand at 501.077 kb on - strand at 501.077 kb on - strand at 501.131 kb on - strand at 501.158 kb on + strand at 501.174 kb on + strand at 501.175 kb on - strand at 501.175 kb on - strand at 501.175 kb on - strand at 501.175 kb on - strand at 501.212 kb on + strand at 501.219 kb on - strand at 501.225 kb on + strand at 501.225 kb on + strand at 501.225 kb on + strand at 501.226 kb on - strand at 501.385 kb on - strand at 501.441 kb on + strand at 501.722 kb on + strand, within ABZR87_RS02340 at 501.722 kb on + strand, within ABZR87_RS02340 at 501.723 kb on - strand, within ABZR87_RS02340 at 501.723 kb on - strand, within ABZR87_RS02340 at 501.723 kb on - strand, within ABZR87_RS02340 at 501.723 kb on - strand, within ABZR87_RS02340 at 501.723 kb on - strand, within ABZR87_RS02340 at 501.921 kb on + strand, within ABZR87_RS02340 at 501.922 kb on - strand, within ABZR87_RS02340 at 501.922 kb on - strand, within ABZR87_RS02340 at 501.922 kb on - strand, within ABZR87_RS02340 at 501.922 kb on - strand, within ABZR87_RS02340 at 501.930 kb on + strand, within ABZR87_RS02340 at 501.930 kb on + strand, within ABZR87_RS02340 at 501.931 kb on - strand, within ABZR87_RS02340 at 501.931 kb on - strand, within ABZR87_RS02340 at 501.931 kb on - strand, within ABZR87_RS02340 at 501.931 kb on - strand, within ABZR87_RS02340 at 501.931 kb on - strand, within ABZR87_RS02340 at 501.931 kb on - strand, within ABZR87_RS02340 at 501.999 kb on + strand, within ABZR87_RS02340 at 502.000 kb on - strand, within ABZR87_RS02340 at 502.000 kb on - strand, within ABZR87_RS02340 at 502.000 kb on - strand, within ABZR87_RS02340 at 502.128 kb on + strand, within ABZR87_RS02340 at 502.128 kb on + strand, within ABZR87_RS02340 at 502.128 kb on + strand, within ABZR87_RS02340 at 502.128 kb on + strand, within ABZR87_RS02340 at 502.128 kb on + strand, within ABZR87_RS02340 at 502.129 kb on - strand, within ABZR87_RS02340 at 502.129 kb on - strand, within ABZR87_RS02340 at 502.129 kb on - strand, within ABZR87_RS02340 at 502.129 kb on - strand, within ABZR87_RS02340 at 502.275 kb on + strand, within ABZR87_RS02340 at 502.275 kb on + strand, within ABZR87_RS02340 at 502.275 kb on + strand, within ABZR87_RS02340 at 502.276 kb on - strand, within ABZR87_RS02340 at 502.276 kb on - strand, within ABZR87_RS02340 at 502.276 kb on - strand, within ABZR87_RS02340 at 502.566 kb on + strand, within ABZR87_RS02340 at 502.567 kb on - strand, within ABZR87_RS02340 at 502.567 kb on - strand, within ABZR87_RS02340 at 502.660 kb on + strand, within ABZR87_RS02340 at 502.660 kb on + strand, within ABZR87_RS02340 at 502.661 kb on - strand, within ABZR87_RS02340 at 502.741 kb on - strand, within ABZR87_RS02340 at 502.741 kb on - strand, within ABZR87_RS02340 at 502.741 kb on - strand, within ABZR87_RS02340 at 502.741 kb on - strand, within ABZR87_RS02340 at 502.741 kb on - strand, within ABZR87_RS02340 at 502.775 kb on + strand, within ABZR87_RS02340 at 502.775 kb on + strand, within ABZR87_RS02340 at 502.775 kb on + strand, within ABZR87_RS02340 at 502.775 kb on + strand, within ABZR87_RS02340 at 502.776 kb on - strand, within ABZR87_RS02340 at 502.776 kb on - strand, within ABZR87_RS02340 at 502.776 kb on - strand, within ABZR87_RS02340 at 502.776 kb on - strand, within ABZR87_RS02340 at 502.788 kb on + strand, within ABZR87_RS02340 at 502.789 kb on - strand, within ABZR87_RS02340 at 502.809 kb on + strand, within ABZR87_RS02340 at 502.809 kb on + strand, within ABZR87_RS02340 at 502.809 kb on + strand, within ABZR87_RS02340 at 502.810 kb on - strand, within ABZR87_RS02340 at 502.810 kb on - strand, within ABZR87_RS02340 at 502.810 kb on - strand, within ABZR87_RS02340 at 502.810 kb on - strand, within ABZR87_RS02340 at 502.810 kb on - strand, within ABZR87_RS02340 at 502.813 kb on + strand, within ABZR87_RS02340 at 502.813 kb on + strand, within ABZR87_RS02340 at 502.814 kb on - strand, within ABZR87_RS02340 at 503.016 kb on - strand at 503.059 kb on + strand at 503.059 kb on + strand at 503.059 kb on + strand at 503.059 kb on + strand at 503.060 kb on - strand at 503.065 kb on + strand at 503.065 kb on + strand at 503.065 kb on + strand at 503.065 kb on + strand at 503.065 kb on + strand at 503.066 kb on - strand at 503.066 kb on - strand at 503.098 kb on + strand, within ABZR87_RS02345 at 503.099 kb on - strand, within ABZR87_RS02345 at 503.429 kb on + strand at 503.451 kb on - strand at 503.472 kb on + strand at 503.472 kb on + strand at 503.472 kb on + strand at 503.473 kb on - strand at 503.473 kb on - strand at 503.473 kb on - strand at 503.473 kb on - strand at 503.484 kb on + strand at 503.484 kb on + strand at 503.485 kb on - strand at 503.491 kb on - strand at 503.491 kb on - strand at 503.491 kb on - strand at 503.511 kb on - strand at 503.547 kb on + strand at 503.547 kb on + strand at 503.563 kb on + strand at 503.564 kb on - strand at 503.613 kb on + strand at 503.613 kb on + strand at 503.614 kb on - strand at 503.614 kb on - strand at 503.614 kb on - strand at 503.614 kb on - strand at 503.643 kb on + strand, within ABZR87_RS02350 at 503.643 kb on + strand, within ABZR87_RS02350 at 503.643 kb on + strand, within ABZR87_RS02350 at 503.643 kb on + strand, within ABZR87_RS02350 at 503.643 kb on + strand, within ABZR87_RS02350 at 503.694 kb on + strand, within ABZR87_RS02350 at 503.695 kb on - strand, within ABZR87_RS02350 at 503.695 kb on - strand, within ABZR87_RS02350 at 503.823 kb on + strand, within ABZR87_RS02350 at 503.823 kb on + strand, within ABZR87_RS02350 at 503.823 kb on + strand, within ABZR87_RS02350 at 503.823 kb on + strand, within ABZR87_RS02350 at 503.824 kb on - strand, within ABZR87_RS02350
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 500,425 + ABZR87_RS02335 0.66 -0.1 500,426 - ABZR87_RS02335 0.66 -2.0 500,450 + ABZR87_RS02335 0.69 +0.8 500,450 + ABZR87_RS02335 0.69 -2.5 500,450 + ABZR87_RS02335 0.69 +0.6 500,451 - ABZR87_RS02335 0.69 +1.2 500,452 + ABZR87_RS02335 0.69 -2.6 500,452 + ABZR87_RS02335 0.69 -0.2 500,452 + ABZR87_RS02335 0.69 +0.5 500,452 + ABZR87_RS02335 0.69 -0.6 500,452 + ABZR87_RS02335 0.69 +0.7 500,452 + ABZR87_RS02335 0.69 -0.5 500,453 - ABZR87_RS02335 0.69 -0.8 500,453 - ABZR87_RS02335 0.69 -2.1 500,453 - ABZR87_RS02335 0.69 +0.2 500,453 - ABZR87_RS02335 0.69 -0.2 500,453 - ABZR87_RS02335 0.69 +0.2 500,453 - ABZR87_RS02335 0.69 -0.6 500,543 - ABZR87_RS02335 0.79 +0.8 500,569 + ABZR87_RS02335 0.81 +0.8 500,569 + ABZR87_RS02335 0.81 -3.1 500,747 + -1.0 500,747 + -1.0 500,747 + -1.4 500,747 + -0.4 500,747 + +0.4 500,747 + -0.4 500,747 + +0.6 500,747 + +1.1 500,747 + -1.5 500,747 + -1.0 500,748 - +2.1 500,748 - -0.7 500,748 - -0.3 500,748 - -0.0 500,748 - -2.3 500,748 - -0.1 500,748 - -0.8 500,877 - -2.1 500,877 - +0.8 500,892 + +0.4 500,892 + +2.4 501,076 + +1.3 501,077 - -1.4 501,077 - +1.1 501,077 - +0.2 501,131 - +0.8 501,158 + -0.7 501,174 + +0.9 501,175 - -0.3 501,175 - +1.0 501,175 - +0.1 501,175 - +0.1 501,212 + -0.5 501,219 - +0.3 501,225 + -0.8 501,225 + -1.0 501,225 + +0.2 501,226 - +0.1 501,385 - -0.0 501,441 + -2.4 501,722 + ABZR87_RS02340 0.21 -2.8 501,722 + ABZR87_RS02340 0.21 -0.0 501,723 - ABZR87_RS02340 0.21 -0.3 501,723 - ABZR87_RS02340 0.21 -1.1 501,723 - ABZR87_RS02340 0.21 -1.4 501,723 - ABZR87_RS02340 0.21 -2.5 501,723 - ABZR87_RS02340 0.21 +0.7 501,921 + ABZR87_RS02340 0.33 +1.2 501,922 - ABZR87_RS02340 0.33 -1.3 501,922 - ABZR87_RS02340 0.33 +0.6 501,922 - ABZR87_RS02340 0.33 -0.9 501,922 - ABZR87_RS02340 0.33 +0.0 501,930 + ABZR87_RS02340 0.33 -1.8 501,930 + ABZR87_RS02340 0.33 +0.2 501,931 - ABZR87_RS02340 0.33 -0.3 501,931 - ABZR87_RS02340 0.33 -0.5 501,931 - ABZR87_RS02340 0.33 -2.0 501,931 - ABZR87_RS02340 0.33 -0.7 501,931 - ABZR87_RS02340 0.33 -0.2 501,931 - ABZR87_RS02340 0.33 -0.1 501,999 + ABZR87_RS02340 0.37 -1.6 502,000 - ABZR87_RS02340 0.38 -0.4 502,000 - ABZR87_RS02340 0.38 -1.3 502,000 - ABZR87_RS02340 0.38 -1.9 502,128 + ABZR87_RS02340 0.45 -0.2 502,128 + ABZR87_RS02340 0.45 -0.6 502,128 + ABZR87_RS02340 0.45 -0.2 502,128 + ABZR87_RS02340 0.45 -1.0 502,128 + ABZR87_RS02340 0.45 -1.9 502,129 - ABZR87_RS02340 0.45 -0.5 502,129 - ABZR87_RS02340 0.45 +0.1 502,129 - ABZR87_RS02340 0.45 -0.9 502,129 - ABZR87_RS02340 0.45 -1.2 502,275 + ABZR87_RS02340 0.54 -0.2 502,275 + ABZR87_RS02340 0.54 -1.2 502,275 + ABZR87_RS02340 0.54 -0.9 502,276 - ABZR87_RS02340 0.54 -1.1 502,276 - ABZR87_RS02340 0.54 -1.5 502,276 - ABZR87_RS02340 0.54 +0.1 502,566 + ABZR87_RS02340 0.72 -0.6 502,567 - ABZR87_RS02340 0.72 -1.6 502,567 - ABZR87_RS02340 0.72 -1.9 502,660 + ABZR87_RS02340 0.78 -0.5 502,660 + ABZR87_RS02340 0.78 -2.1 502,661 - ABZR87_RS02340 0.78 -1.4 502,741 - ABZR87_RS02340 0.83 +0.0 502,741 - ABZR87_RS02340 0.83 -1.0 502,741 - ABZR87_RS02340 0.83 -2.7 502,741 - ABZR87_RS02340 0.83 -0.9 502,741 - ABZR87_RS02340 0.83 -0.6 502,775 + ABZR87_RS02340 0.85 -0.8 502,775 + ABZR87_RS02340 0.85 -1.7 502,775 + ABZR87_RS02340 0.85 -0.1 502,775 + ABZR87_RS02340 0.85 -0.7 502,776 - ABZR87_RS02340 0.85 -0.2 502,776 - ABZR87_RS02340 0.85 -1.1 502,776 - ABZR87_RS02340 0.85 -0.2 502,776 - ABZR87_RS02340 0.85 -2.2 502,788 + ABZR87_RS02340 0.85 -1.0 502,789 - ABZR87_RS02340 0.86 -1.5 502,809 + ABZR87_RS02340 0.87 -0.4 502,809 + ABZR87_RS02340 0.87 -1.3 502,809 + ABZR87_RS02340 0.87 -0.7 502,810 - ABZR87_RS02340 0.87 -0.3 502,810 - ABZR87_RS02340 0.87 +0.2 502,810 - ABZR87_RS02340 0.87 -0.2 502,810 - ABZR87_RS02340 0.87 -0.3 502,810 - ABZR87_RS02340 0.87 -2.8 502,813 + ABZR87_RS02340 0.87 -2.8 502,813 + ABZR87_RS02340 0.87 -2.0 502,814 - ABZR87_RS02340 0.87 -1.7 503,016 - -1.8 503,059 + +1.2 503,059 + -0.7 503,059 + -0.1 503,059 + -0.3 503,060 - -0.4 503,065 + -0.3 503,065 + -1.7 503,065 + -1.3 503,065 + -1.1 503,065 + -2.4 503,066 - -1.1 503,066 - -1.5 503,098 + ABZR87_RS02345 0.19 -1.0 503,099 - ABZR87_RS02345 0.20 -0.1 503,429 + -1.1 503,451 - -0.1 503,472 + -0.2 503,472 + -1.1 503,472 + -0.6 503,473 - -0.8 503,473 - -0.3 503,473 - -1.5 503,473 - -0.6 503,484 + -0.4 503,484 + -0.0 503,485 - +0.5 503,491 - -0.9 503,491 - -1.7 503,491 - -0.2 503,511 - -0.2 503,547 + -0.9 503,547 + -1.2 503,563 + -0.6 503,564 - -1.0 503,613 + +0.6 503,613 + -1.8 503,614 - -1.0 503,614 - -0.9 503,614 - +0.1 503,614 - +0.4 503,643 + ABZR87_RS02350 0.11 +0.2 503,643 + ABZR87_RS02350 0.11 -0.9 503,643 + ABZR87_RS02350 0.11 +0.1 503,643 + ABZR87_RS02350 0.11 +0.1 503,643 + ABZR87_RS02350 0.11 -0.4 503,694 + ABZR87_RS02350 0.15 -1.1 503,695 - ABZR87_RS02350 0.15 -0.8 503,695 - ABZR87_RS02350 0.15 +0.3 503,823 + ABZR87_RS02350 0.24 +1.5 503,823 + ABZR87_RS02350 0.24 +0.8 503,823 + ABZR87_RS02350 0.24 -1.4 503,823 + ABZR87_RS02350 0.24 -3.1 503,824 - ABZR87_RS02350 0.24 +0.4
Or see this region's nucleotide sequence