Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS02095 and ABZR87_RS02100 are separated by 83 nucleotides ABZR87_RS02100 and ABZR87_RS02105 are separated by 4 nucleotides ABZR87_RS02105 and ABZR87_RS02110 are separated by 204 nucleotides
ABZR87_RS02095: ABZR87_RS02095 - enoyl-CoA hydratase, at 451,652 to 452,428
_RS02095
ABZR87_RS02100: ABZR87_RS02100 - 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase PaaG, at 452,512 to 453,300
_RS02100
ABZR87_RS02105: ABZR87_RS02105 - hydroxyphenylacetyl-CoA thioesterase PaaI, at 453,305 to 453,772
_RS02105
ABZR87_RS02110: ABZR87_RS02110 - phenylacetate--CoA ligase PaaK, at 453,977 to 455,281
_RS02110
Position (kb) 453
454 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 452.377 kb on + strand at 452.377 kb on + strand at 452.377 kb on + strand at 452.378 kb on - strand at 452.378 kb on - strand at 452.378 kb on - strand at 452.422 kb on - strand at 452.422 kb on - strand at 452.452 kb on + strand at 452.452 kb on + strand at 452.918 kb on + strand, within ABZR87_RS02100 at 452.918 kb on + strand, within ABZR87_RS02100 at 452.918 kb on + strand, within ABZR87_RS02100 at 452.919 kb on - strand, within ABZR87_RS02100 at 452.919 kb on - strand, within ABZR87_RS02100 at 452.919 kb on - strand, within ABZR87_RS02100 at 453.241 kb on + strand at 453.278 kb on + strand at 453.278 kb on + strand at 453.298 kb on + strand at 453.298 kb on + strand at 453.298 kb on + strand at 453.298 kb on + strand at 453.299 kb on - strand at 453.299 kb on - strand at 453.299 kb on - strand at 453.299 kb on - strand at 453.431 kb on + strand, within ABZR87_RS02105 at 453.432 kb on - strand, within ABZR87_RS02105 at 453.432 kb on - strand, within ABZR87_RS02105 at 453.548 kb on - strand, within ABZR87_RS02105 at 453.662 kb on + strand, within ABZR87_RS02105 at 453.662 kb on + strand, within ABZR87_RS02105 at 453.662 kb on + strand, within ABZR87_RS02105 at 453.662 kb on + strand, within ABZR87_RS02105 at 453.662 kb on + strand, within ABZR87_RS02105 at 453.662 kb on + strand, within ABZR87_RS02105 at 453.663 kb on - strand, within ABZR87_RS02105 at 453.663 kb on - strand, within ABZR87_RS02105 at 453.663 kb on - strand, within ABZR87_RS02105 at 453.663 kb on - strand, within ABZR87_RS02105 at 453.663 kb on - strand, within ABZR87_RS02105 at 453.663 kb on - strand, within ABZR87_RS02105 at 453.663 kb on - strand, within ABZR87_RS02105 at 453.663 kb on - strand, within ABZR87_RS02105 at 453.669 kb on + strand, within ABZR87_RS02105 at 453.669 kb on + strand, within ABZR87_RS02105 at 453.669 kb on + strand, within ABZR87_RS02105 at 453.669 kb on + strand, within ABZR87_RS02105 at 453.669 kb on + strand, within ABZR87_RS02105 at 453.669 kb on + strand, within ABZR87_RS02105 at 453.670 kb on - strand, within ABZR87_RS02105 at 453.681 kb on - strand, within ABZR87_RS02105 at 453.771 kb on - strand at 453.785 kb on - strand at 453.785 kb on - strand at 453.785 kb on - strand at 453.786 kb on + strand at 453.786 kb on + strand at 453.786 kb on + strand at 453.786 kb on + strand at 453.786 kb on + strand at 453.786 kb on + strand at 453.786 kb on + strand at 453.786 kb on + strand at 453.786 kb on + strand at 453.786 kb on + strand at 453.786 kb on + strand at 453.786 kb on + strand at 453.786 kb on + strand at 453.787 kb on - strand at 453.787 kb on - strand at 453.787 kb on - strand at 453.787 kb on - strand at 453.787 kb on - strand at 453.787 kb on - strand at 453.816 kb on + strand at 453.816 kb on + strand at 453.817 kb on - strand at 453.817 kb on - strand at 453.886 kb on + strand at 453.886 kb on + strand at 453.886 kb on + strand at 453.886 kb on + strand at 453.886 kb on + strand at 453.886 kb on + strand at 453.886 kb on + strand at 453.886 kb on + strand at 453.886 kb on + strand at 453.886 kb on + strand at 453.886 kb on + strand at 453.886 kb on + strand at 453.886 kb on + strand at 453.886 kb on + strand at 453.886 kb on + strand at 453.886 kb on + strand at 453.886 kb on + strand at 453.886 kb on + strand at 453.886 kb on + strand at 453.887 kb on - strand at 453.887 kb on - strand at 453.887 kb on - strand at 453.887 kb on - strand at 453.887 kb on - strand at 453.887 kb on - strand at 453.887 kb on - strand at 453.887 kb on - strand at 453.887 kb on - strand at 453.887 kb on - strand at 453.887 kb on - strand at 453.887 kb on - strand at 453.887 kb on - strand at 453.887 kb on - strand at 453.887 kb on - strand at 453.887 kb on - strand at 453.887 kb on - strand at 453.887 kb on - strand at 453.894 kb on + strand at 453.895 kb on - strand at 453.932 kb on + strand at 453.932 kb on + strand at 454.085 kb on + strand at 454.085 kb on + strand at 454.085 kb on + strand at 454.085 kb on + strand at 454.085 kb on + strand at 454.085 kb on + strand at 454.085 kb on + strand at 454.086 kb on - strand at 454.086 kb on - strand at 454.086 kb on - strand at 454.086 kb on - strand at 454.086 kb on - strand at 454.208 kb on + strand, within ABZR87_RS02110 at 454.208 kb on + strand, within ABZR87_RS02110 at 454.208 kb on + strand, within ABZR87_RS02110 at 454.208 kb on + strand, within ABZR87_RS02110 at 454.208 kb on + strand, within ABZR87_RS02110 at 454.209 kb on - strand, within ABZR87_RS02110 at 454.209 kb on - strand, within ABZR87_RS02110 at 454.209 kb on - strand, within ABZR87_RS02110 at 454.209 kb on - strand, within ABZR87_RS02110 at 454.209 kb on - strand, within ABZR87_RS02110 at 454.299 kb on - strand, within ABZR87_RS02110 at 454.318 kb on + strand, within ABZR87_RS02110 at 454.318 kb on + strand, within ABZR87_RS02110 at 454.318 kb on + strand, within ABZR87_RS02110 at 454.318 kb on + strand, within ABZR87_RS02110 at 454.318 kb on + strand, within ABZR87_RS02110 at 454.318 kb on + strand, within ABZR87_RS02110 at 454.318 kb on + strand, within ABZR87_RS02110 at 454.318 kb on + strand, within ABZR87_RS02110 at 454.318 kb on + strand, within ABZR87_RS02110 at 454.318 kb on + strand, within ABZR87_RS02110 at 454.319 kb on - strand, within ABZR87_RS02110 at 454.319 kb on - strand, within ABZR87_RS02110 at 454.319 kb on - strand, within ABZR87_RS02110 at 454.319 kb on - strand, within ABZR87_RS02110 at 454.319 kb on - strand, within ABZR87_RS02110 at 454.319 kb on - strand, within ABZR87_RS02110 at 454.319 kb on - strand, within ABZR87_RS02110 at 454.391 kb on + strand, within ABZR87_RS02110 at 454.392 kb on - strand, within ABZR87_RS02110 at 454.392 kb on - strand, within ABZR87_RS02110 at 454.397 kb on + strand, within ABZR87_RS02110 at 454.398 kb on - strand, within ABZR87_RS02110 at 454.544 kb on + strand, within ABZR87_RS02110 at 454.544 kb on + strand, within ABZR87_RS02110 at 454.544 kb on + strand, within ABZR87_RS02110 at 454.544 kb on + strand, within ABZR87_RS02110 at 454.544 kb on + strand, within ABZR87_RS02110 at 454.544 kb on + strand, within ABZR87_RS02110 at 454.544 kb on + strand, within ABZR87_RS02110 at 454.544 kb on + strand, within ABZR87_RS02110 at 454.544 kb on + strand, within ABZR87_RS02110 at 454.545 kb on - strand, within ABZR87_RS02110 at 454.545 kb on - strand, within ABZR87_RS02110 at 454.545 kb on - strand, within ABZR87_RS02110 at 454.545 kb on - strand, within ABZR87_RS02110 at 454.545 kb on - strand, within ABZR87_RS02110 at 454.545 kb on - strand, within ABZR87_RS02110 at 454.545 kb on - strand, within ABZR87_RS02110 at 454.545 kb on - strand, within ABZR87_RS02110 at 454.545 kb on - strand, within ABZR87_RS02110 at 454.545 kb on - strand, within ABZR87_RS02110 at 454.690 kb on + strand, within ABZR87_RS02110 at 454.690 kb on + strand, within ABZR87_RS02110 at 454.691 kb on - strand, within ABZR87_RS02110 at 454.691 kb on - strand, within ABZR87_RS02110 at 454.691 kb on - strand, within ABZR87_RS02110 at 454.691 kb on - strand, within ABZR87_RS02110 at 454.691 kb on - strand, within ABZR87_RS02110 at 454.694 kb on + strand, within ABZR87_RS02110 at 454.694 kb on + strand, within ABZR87_RS02110 at 454.695 kb on - strand, within ABZR87_RS02110 at 454.695 kb on - strand, within ABZR87_RS02110 at 454.695 kb on - strand, within ABZR87_RS02110 at 454.695 kb on - strand, within ABZR87_RS02110 at 454.695 kb on - strand, within ABZR87_RS02110
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 452,377 + +0.8 452,377 + +0.5 452,377 + +0.5 452,378 - +0.5 452,378 - -0.3 452,378 - +0.1 452,422 - +1.1 452,422 - -0.7 452,452 + +1.1 452,452 + +0.9 452,918 + ABZR87_RS02100 0.51 -0.0 452,918 + ABZR87_RS02100 0.51 +0.1 452,918 + ABZR87_RS02100 0.51 +0.4 452,919 - ABZR87_RS02100 0.52 +0.9 452,919 - ABZR87_RS02100 0.52 +0.6 452,919 - ABZR87_RS02100 0.52 +0.1 453,241 + -0.4 453,278 + -1.1 453,278 + -1.3 453,298 + -0.6 453,298 + -0.0 453,298 + -0.8 453,298 + +0.3 453,299 - +1.6 453,299 - +0.4 453,299 - +0.3 453,299 - -0.1 453,431 + ABZR87_RS02105 0.27 -1.2 453,432 - ABZR87_RS02105 0.27 -0.4 453,432 - ABZR87_RS02105 0.27 +0.3 453,548 - ABZR87_RS02105 0.52 -0.3 453,662 + ABZR87_RS02105 0.76 -0.3 453,662 + ABZR87_RS02105 0.76 +0.5 453,662 + ABZR87_RS02105 0.76 -0.3 453,662 + ABZR87_RS02105 0.76 +0.5 453,662 + ABZR87_RS02105 0.76 +0.1 453,662 + ABZR87_RS02105 0.76 -0.8 453,663 - ABZR87_RS02105 0.76 -0.1 453,663 - ABZR87_RS02105 0.76 -0.4 453,663 - ABZR87_RS02105 0.76 -0.2 453,663 - ABZR87_RS02105 0.76 -0.3 453,663 - ABZR87_RS02105 0.76 -0.2 453,663 - ABZR87_RS02105 0.76 -0.6 453,663 - ABZR87_RS02105 0.76 -0.7 453,663 - ABZR87_RS02105 0.76 +0.1 453,669 + ABZR87_RS02105 0.78 -0.2 453,669 + ABZR87_RS02105 0.78 -0.5 453,669 + ABZR87_RS02105 0.78 -0.1 453,669 + ABZR87_RS02105 0.78 -0.5 453,669 + ABZR87_RS02105 0.78 +1.0 453,669 + ABZR87_RS02105 0.78 -0.7 453,670 - ABZR87_RS02105 0.78 -0.8 453,681 - ABZR87_RS02105 0.80 -0.2 453,771 - -1.2 453,785 - -0.2 453,785 - -0.1 453,785 - +0.1 453,786 + -1.5 453,786 + +0.6 453,786 + -2.4 453,786 + +0.7 453,786 + -0.6 453,786 + +0.6 453,786 + +0.2 453,786 + +0.3 453,786 + +0.0 453,786 + +0.4 453,786 + -1.9 453,786 + -0.2 453,786 + +0.1 453,787 - -0.1 453,787 - -0.4 453,787 - -1.2 453,787 - -0.3 453,787 - +0.4 453,787 - +0.3 453,816 + +0.4 453,816 + +0.5 453,817 - -0.4 453,817 - +0.1 453,886 + +0.3 453,886 + -0.4 453,886 + -0.4 453,886 + +3.8 453,886 + -0.3 453,886 + -0.7 453,886 + -0.7 453,886 + +1.6 453,886 + +0.6 453,886 + -0.8 453,886 + -0.1 453,886 + +0.5 453,886 + -0.9 453,886 + +0.3 453,886 + -0.1 453,886 + +0.3 453,886 + -0.7 453,886 + +0.4 453,886 + -0.2 453,887 - -0.1 453,887 - +1.4 453,887 - +0.6 453,887 - +0.8 453,887 - -0.1 453,887 - +0.0 453,887 - +0.5 453,887 - -1.6 453,887 - +0.2 453,887 - +0.2 453,887 - +2.3 453,887 - +0.2 453,887 - -0.1 453,887 - -0.4 453,887 - +1.0 453,887 - -0.4 453,887 - -1.4 453,887 - +0.8 453,894 + -0.2 453,895 - -0.7 453,932 + -0.4 453,932 + -1.2 454,085 + -0.6 454,085 + +0.3 454,085 + -0.2 454,085 + +0.5 454,085 + +0.2 454,085 + +1.1 454,085 + +0.0 454,086 - +0.1 454,086 - -0.1 454,086 - -0.7 454,086 - -0.5 454,086 - +0.1 454,208 + ABZR87_RS02110 0.18 -0.5 454,208 + ABZR87_RS02110 0.18 +0.4 454,208 + ABZR87_RS02110 0.18 -0.3 454,208 + ABZR87_RS02110 0.18 -1.2 454,208 + ABZR87_RS02110 0.18 +0.5 454,209 - ABZR87_RS02110 0.18 +0.6 454,209 - ABZR87_RS02110 0.18 -0.6 454,209 - ABZR87_RS02110 0.18 -0.7 454,209 - ABZR87_RS02110 0.18 -0.4 454,209 - ABZR87_RS02110 0.18 -0.4 454,299 - ABZR87_RS02110 0.25 +0.3 454,318 + ABZR87_RS02110 0.26 +0.6 454,318 + ABZR87_RS02110 0.26 +0.6 454,318 + ABZR87_RS02110 0.26 +0.3 454,318 + ABZR87_RS02110 0.26 -2.2 454,318 + ABZR87_RS02110 0.26 +0.3 454,318 + ABZR87_RS02110 0.26 -0.7 454,318 + ABZR87_RS02110 0.26 -0.1 454,318 + ABZR87_RS02110 0.26 -0.4 454,318 + ABZR87_RS02110 0.26 -0.6 454,318 + ABZR87_RS02110 0.26 +0.1 454,319 - ABZR87_RS02110 0.26 -0.4 454,319 - ABZR87_RS02110 0.26 +0.7 454,319 - ABZR87_RS02110 0.26 -0.2 454,319 - ABZR87_RS02110 0.26 +0.6 454,319 - ABZR87_RS02110 0.26 +0.2 454,319 - ABZR87_RS02110 0.26 -1.5 454,319 - ABZR87_RS02110 0.26 -0.1 454,391 + ABZR87_RS02110 0.32 +0.1 454,392 - ABZR87_RS02110 0.32 +0.2 454,392 - ABZR87_RS02110 0.32 +0.5 454,397 + ABZR87_RS02110 0.32 -0.0 454,398 - ABZR87_RS02110 0.32 +0.3 454,544 + ABZR87_RS02110 0.43 +1.1 454,544 + ABZR87_RS02110 0.43 +0.1 454,544 + ABZR87_RS02110 0.43 -0.4 454,544 + ABZR87_RS02110 0.43 +0.3 454,544 + ABZR87_RS02110 0.43 -0.4 454,544 + ABZR87_RS02110 0.43 -1.4 454,544 + ABZR87_RS02110 0.43 -1.0 454,544 + ABZR87_RS02110 0.43 -0.3 454,544 + ABZR87_RS02110 0.43 +0.5 454,545 - ABZR87_RS02110 0.44 -0.3 454,545 - ABZR87_RS02110 0.44 -0.3 454,545 - ABZR87_RS02110 0.44 -1.2 454,545 - ABZR87_RS02110 0.44 -0.2 454,545 - ABZR87_RS02110 0.44 -0.1 454,545 - ABZR87_RS02110 0.44 -0.0 454,545 - ABZR87_RS02110 0.44 -0.8 454,545 - ABZR87_RS02110 0.44 -0.4 454,545 - ABZR87_RS02110 0.44 -0.9 454,545 - ABZR87_RS02110 0.44 +0.2 454,690 + ABZR87_RS02110 0.55 +0.3 454,690 + ABZR87_RS02110 0.55 -0.9 454,691 - ABZR87_RS02110 0.55 -0.1 454,691 - ABZR87_RS02110 0.55 -0.0 454,691 - ABZR87_RS02110 0.55 -0.5 454,691 - ABZR87_RS02110 0.55 +0.6 454,691 - ABZR87_RS02110 0.55 +0.1 454,694 + ABZR87_RS02110 0.55 -1.0 454,694 + ABZR87_RS02110 0.55 +0.4 454,695 - ABZR87_RS02110 0.55 -0.4 454,695 - ABZR87_RS02110 0.55 +1.0 454,695 - ABZR87_RS02110 0.55 +3.8 454,695 - ABZR87_RS02110 0.55 -0.4 454,695 - ABZR87_RS02110 0.55 +0.0
Or see this region's nucleotide sequence