Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS01080 and ABZR87_RS01085 are separated by 94 nucleotides ABZR87_RS01085 and ABZR87_RS01090 overlap by 4 nucleotides
ABZR87_RS01080: ABZR87_RS01080 - S8 family serine peptidase, at 217,347 to 219,401
_RS01080
ABZR87_RS01085: ABZR87_RS01085 - rhombosortase, at 219,496 to 220,116
_RS01085
ABZR87_RS01090: ABZR87_RS01090 - bifunctional [glutamate--ammonia ligase]-adenylyl-L-tyrosine phosphorylase/[glutamate--ammonia-ligase] adenylyltransferase, at 220,113 to 222,956
_RS01090
Position (kb)
219
220
221 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 218.583 kb on + strand, within ABZR87_RS01080 at 218.603 kb on + strand, within ABZR87_RS01080 at 218.604 kb on - strand, within ABZR87_RS01080 at 218.604 kb on - strand, within ABZR87_RS01080 at 218.715 kb on + strand, within ABZR87_RS01080 at 218.715 kb on + strand, within ABZR87_RS01080 at 218.715 kb on + strand, within ABZR87_RS01080 at 218.715 kb on + strand, within ABZR87_RS01080 at 218.716 kb on - strand, within ABZR87_RS01080 at 218.716 kb on - strand, within ABZR87_RS01080 at 218.716 kb on - strand, within ABZR87_RS01080 at 218.716 kb on - strand, within ABZR87_RS01080 at 218.718 kb on + strand, within ABZR87_RS01080 at 218.718 kb on + strand, within ABZR87_RS01080 at 218.718 kb on + strand, within ABZR87_RS01080 at 218.718 kb on + strand, within ABZR87_RS01080 at 218.718 kb on + strand, within ABZR87_RS01080 at 218.719 kb on - strand, within ABZR87_RS01080 at 218.719 kb on - strand, within ABZR87_RS01080 at 218.719 kb on - strand, within ABZR87_RS01080 at 218.719 kb on - strand, within ABZR87_RS01080 at 218.859 kb on - strand, within ABZR87_RS01080 at 218.977 kb on + strand, within ABZR87_RS01080 at 218.999 kb on + strand, within ABZR87_RS01080 at 219.000 kb on - strand, within ABZR87_RS01080 at 219.026 kb on + strand, within ABZR87_RS01080 at 219.090 kb on - strand, within ABZR87_RS01080 at 219.117 kb on + strand, within ABZR87_RS01080 at 219.118 kb on - strand, within ABZR87_RS01080 at 219.118 kb on - strand, within ABZR87_RS01080 at 219.118 kb on - strand, within ABZR87_RS01080 at 219.118 kb on - strand, within ABZR87_RS01080 at 219.153 kb on + strand, within ABZR87_RS01080 at 219.153 kb on + strand, within ABZR87_RS01080 at 219.153 kb on + strand, within ABZR87_RS01080 at 219.154 kb on - strand, within ABZR87_RS01080 at 219.408 kb on - strand at 219.408 kb on - strand at 219.589 kb on + strand, within ABZR87_RS01085 at 219.589 kb on + strand, within ABZR87_RS01085 at 219.589 kb on + strand, within ABZR87_RS01085 at 219.590 kb on - strand, within ABZR87_RS01085 at 220.011 kb on - strand, within ABZR87_RS01085 at 220.011 kb on - strand, within ABZR87_RS01085 at 220.026 kb on - strand, within ABZR87_RS01085 at 220.178 kb on + strand at 220.178 kb on + strand at 220.179 kb on - strand at 220.179 kb on - strand at 220.250 kb on - strand at 220.535 kb on + strand, within ABZR87_RS01090 at 220.742 kb on + strand, within ABZR87_RS01090 at 220.786 kb on + strand, within ABZR87_RS01090 at 220.786 kb on + strand, within ABZR87_RS01090 at 220.787 kb on - strand, within ABZR87_RS01090 at 220.787 kb on - strand, within ABZR87_RS01090 at 220.820 kb on - strand, within ABZR87_RS01090 at 220.850 kb on - strand, within ABZR87_RS01090 at 220.879 kb on + strand, within ABZR87_RS01090 at 220.880 kb on - strand, within ABZR87_RS01090
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 218,583 + ABZR87_RS01080 0.60 +0.5 218,603 + ABZR87_RS01080 0.61 +1.2 218,604 - ABZR87_RS01080 0.61 +0.6 218,604 - ABZR87_RS01080 0.61 -0.1 218,715 + ABZR87_RS01080 0.67 +0.7 218,715 + ABZR87_RS01080 0.67 -0.6 218,715 + ABZR87_RS01080 0.67 -2.7 218,715 + ABZR87_RS01080 0.67 +1.7 218,716 - ABZR87_RS01080 0.67 +0.1 218,716 - ABZR87_RS01080 0.67 +0.2 218,716 - ABZR87_RS01080 0.67 +1.2 218,716 - ABZR87_RS01080 0.67 +0.5 218,718 + ABZR87_RS01080 0.67 -1.1 218,718 + ABZR87_RS01080 0.67 -0.0 218,718 + ABZR87_RS01080 0.67 -0.6 218,718 + ABZR87_RS01080 0.67 -1.1 218,718 + ABZR87_RS01080 0.67 -0.4 218,719 - ABZR87_RS01080 0.67 +0.1 218,719 - ABZR87_RS01080 0.67 -0.4 218,719 - ABZR87_RS01080 0.67 +0.4 218,719 - ABZR87_RS01080 0.67 +0.3 218,859 - ABZR87_RS01080 0.74 +0.1 218,977 + ABZR87_RS01080 0.79 +0.8 218,999 + ABZR87_RS01080 0.80 +1.6 219,000 - ABZR87_RS01080 0.80 +0.8 219,026 + ABZR87_RS01080 0.82 +0.5 219,090 - ABZR87_RS01080 0.85 +0.9 219,117 + ABZR87_RS01080 0.86 -0.3 219,118 - ABZR87_RS01080 0.86 +0.3 219,118 - ABZR87_RS01080 0.86 +1.0 219,118 - ABZR87_RS01080 0.86 -0.0 219,118 - ABZR87_RS01080 0.86 -0.3 219,153 + ABZR87_RS01080 0.88 +0.9 219,153 + ABZR87_RS01080 0.88 -0.5 219,153 + ABZR87_RS01080 0.88 +4.7 219,154 - ABZR87_RS01080 0.88 +0.1 219,408 - +0.7 219,408 - +0.6 219,589 + ABZR87_RS01085 0.15 -0.9 219,589 + ABZR87_RS01085 0.15 -0.4 219,589 + ABZR87_RS01085 0.15 -2.7 219,590 - ABZR87_RS01085 0.15 +0.3 220,011 - ABZR87_RS01085 0.83 -0.5 220,011 - ABZR87_RS01085 0.83 -1.1 220,026 - ABZR87_RS01085 0.85 -0.4 220,178 + +0.2 220,178 + -1.4 220,179 - +0.1 220,179 - -0.8 220,250 - -2.1 220,535 + ABZR87_RS01090 0.15 -0.7 220,742 + ABZR87_RS01090 0.22 +0.4 220,786 + ABZR87_RS01090 0.24 +0.1 220,786 + ABZR87_RS01090 0.24 -0.1 220,787 - ABZR87_RS01090 0.24 +0.1 220,787 - ABZR87_RS01090 0.24 -0.5 220,820 - ABZR87_RS01090 0.25 -1.1 220,850 - ABZR87_RS01090 0.26 -1.1 220,879 + ABZR87_RS01090 0.27 -0.4 220,880 - ABZR87_RS01090 0.27 -0.6
Or see this region's nucleotide sequence