Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS01015 and ABZR87_RS01020 overlap by 8 nucleotides ABZR87_RS01020 and ABZR87_RS01025 are separated by 2 nucleotides ABZR87_RS01025 and ABZR87_RS01030 are separated by 25 nucleotides
ABZR87_RS01015: ABZR87_RS01015 - imidazolonepropionase, at 201,609 to 202,844
_RS01015
ABZR87_RS01020: ABZR87_RS01020 - formimidoylglutamase, at 202,837 to 203,814
_RS01020
ABZR87_RS01025: ABZR87_RS01025 - histidine ammonia-lyase, at 203,817 to 205,373
_RS01025
ABZR87_RS01030: ABZR87_RS01030 - urocanate hydratase, at 205,399 to 207,117
_RS01030
Position (kb)
203
204
205
206 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 203.090 kb on + strand, within ABZR87_RS01020 at 203.091 kb on - strand, within ABZR87_RS01020 at 203.228 kb on + strand, within ABZR87_RS01020 at 203.228 kb on + strand, within ABZR87_RS01020 at 203.228 kb on + strand, within ABZR87_RS01020 at 203.229 kb on - strand, within ABZR87_RS01020 at 203.229 kb on - strand, within ABZR87_RS01020 at 203.229 kb on - strand, within ABZR87_RS01020 at 203.252 kb on + strand, within ABZR87_RS01020 at 203.252 kb on + strand, within ABZR87_RS01020 at 203.253 kb on - strand, within ABZR87_RS01020 at 203.341 kb on + strand, within ABZR87_RS01020 at 203.370 kb on + strand, within ABZR87_RS01020 at 203.929 kb on + strand at 203.929 kb on + strand at 203.929 kb on + strand at 203.929 kb on + strand at 203.930 kb on - strand at 203.930 kb on - strand at 203.932 kb on + strand at 203.932 kb on + strand at 203.932 kb on + strand at 204.385 kb on - strand, within ABZR87_RS01025 at 204.403 kb on + strand, within ABZR87_RS01025 at 204.403 kb on + strand, within ABZR87_RS01025 at 204.404 kb on - strand, within ABZR87_RS01025 at 204.512 kb on + strand, within ABZR87_RS01025 at 204.512 kb on + strand, within ABZR87_RS01025 at 204.513 kb on - strand, within ABZR87_RS01025 at 204.513 kb on - strand, within ABZR87_RS01025 at 204.514 kb on + strand, within ABZR87_RS01025 at 204.514 kb on + strand, within ABZR87_RS01025 at 204.514 kb on + strand, within ABZR87_RS01025 at 204.515 kb on - strand, within ABZR87_RS01025 at 204.515 kb on - strand, within ABZR87_RS01025 at 204.586 kb on + strand, within ABZR87_RS01025 at 205.373 kb on + strand at 205.373 kb on + strand at 205.373 kb on + strand at 205.373 kb on + strand at 205.373 kb on + strand at 205.374 kb on - strand at 205.374 kb on - strand at 205.374 kb on - strand at 205.374 kb on - strand at 205.395 kb on + strand at 205.396 kb on - strand at 205.396 kb on - strand at 205.396 kb on - strand at 205.396 kb on - strand at 205.396 kb on - strand at 205.400 kb on + strand at 205.401 kb on - strand at 205.401 kb on - strand at 205.577 kb on + strand, within ABZR87_RS01030 at 205.578 kb on - strand, within ABZR87_RS01030 at 205.949 kb on + strand, within ABZR87_RS01030 at 205.949 kb on + strand, within ABZR87_RS01030 at 205.949 kb on + strand, within ABZR87_RS01030 at 205.949 kb on + strand, within ABZR87_RS01030 at 205.949 kb on + strand, within ABZR87_RS01030 at 205.949 kb on + strand, within ABZR87_RS01030 at 205.949 kb on + strand, within ABZR87_RS01030 at 205.949 kb on + strand, within ABZR87_RS01030 at 205.949 kb on + strand, within ABZR87_RS01030 at 205.949 kb on + strand, within ABZR87_RS01030 at 205.950 kb on - strand, within ABZR87_RS01030 at 205.950 kb on - strand, within ABZR87_RS01030 at 205.950 kb on - strand, within ABZR87_RS01030 at 205.950 kb on - strand, within ABZR87_RS01030 at 205.950 kb on - strand, within ABZR87_RS01030 at 206.024 kb on + strand, within ABZR87_RS01030 at 206.024 kb on + strand, within ABZR87_RS01030 at 206.024 kb on + strand, within ABZR87_RS01030 at 206.024 kb on + strand, within ABZR87_RS01030 at 206.025 kb on - strand, within ABZR87_RS01030 at 206.025 kb on - strand, within ABZR87_RS01030 at 206.102 kb on + strand, within ABZR87_RS01030 at 206.103 kb on - strand, within ABZR87_RS01030 at 206.256 kb on - strand, within ABZR87_RS01030
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 203,090 + ABZR87_RS01020 0.26 -0.6 203,091 - ABZR87_RS01020 0.26 -0.2 203,228 + ABZR87_RS01020 0.40 +0.0 203,228 + ABZR87_RS01020 0.40 -1.3 203,228 + ABZR87_RS01020 0.40 +0.7 203,229 - ABZR87_RS01020 0.40 -1.3 203,229 - ABZR87_RS01020 0.40 -0.6 203,229 - ABZR87_RS01020 0.40 -0.4 203,252 + ABZR87_RS01020 0.42 +1.5 203,252 + ABZR87_RS01020 0.42 -0.2 203,253 - ABZR87_RS01020 0.43 +0.2 203,341 + ABZR87_RS01020 0.52 -1.0 203,370 + ABZR87_RS01020 0.54 +0.6 203,929 + +1.3 203,929 + -1.7 203,929 + -0.9 203,929 + -0.3 203,930 - -0.4 203,930 - +0.4 203,932 + +0.2 203,932 + +0.6 203,932 + +0.8 204,385 - ABZR87_RS01025 0.36 +1.0 204,403 + ABZR87_RS01025 0.38 -0.3 204,403 + ABZR87_RS01025 0.38 +0.3 204,404 - ABZR87_RS01025 0.38 -0.0 204,512 + ABZR87_RS01025 0.45 +0.4 204,512 + ABZR87_RS01025 0.45 +0.3 204,513 - ABZR87_RS01025 0.45 +0.7 204,513 - ABZR87_RS01025 0.45 -0.1 204,514 + ABZR87_RS01025 0.45 -0.3 204,514 + ABZR87_RS01025 0.45 +0.0 204,514 + ABZR87_RS01025 0.45 -0.3 204,515 - ABZR87_RS01025 0.45 -0.7 204,515 - ABZR87_RS01025 0.45 -0.2 204,586 + ABZR87_RS01025 0.49 -0.5 205,373 + -0.9 205,373 + -0.3 205,373 + -0.7 205,373 + -0.5 205,373 + +0.2 205,374 - -0.3 205,374 - +0.1 205,374 - +0.1 205,374 - +0.7 205,395 + -0.1 205,396 - -1.9 205,396 - -3.0 205,396 - -0.1 205,396 - +0.1 205,396 - +0.4 205,400 + -0.2 205,401 - -0.4 205,401 - -2.4 205,577 + ABZR87_RS01030 0.10 +0.6 205,578 - ABZR87_RS01030 0.10 -1.0 205,949 + ABZR87_RS01030 0.32 +0.3 205,949 + ABZR87_RS01030 0.32 -0.4 205,949 + ABZR87_RS01030 0.32 -1.6 205,949 + ABZR87_RS01030 0.32 +0.7 205,949 + ABZR87_RS01030 0.32 -0.4 205,949 + ABZR87_RS01030 0.32 +0.3 205,949 + ABZR87_RS01030 0.32 +0.4 205,949 + ABZR87_RS01030 0.32 +0.9 205,949 + ABZR87_RS01030 0.32 +0.1 205,949 + ABZR87_RS01030 0.32 -1.4 205,950 - ABZR87_RS01030 0.32 +1.9 205,950 - ABZR87_RS01030 0.32 -0.7 205,950 - ABZR87_RS01030 0.32 -0.3 205,950 - ABZR87_RS01030 0.32 +0.8 205,950 - ABZR87_RS01030 0.32 -0.2 206,024 + ABZR87_RS01030 0.36 -0.7 206,024 + ABZR87_RS01030 0.36 +0.8 206,024 + ABZR87_RS01030 0.36 -1.0 206,024 + ABZR87_RS01030 0.36 -1.8 206,025 - ABZR87_RS01030 0.36 -0.4 206,025 - ABZR87_RS01030 0.36 +0.7 206,102 + ABZR87_RS01030 0.41 +1.3 206,103 - ABZR87_RS01030 0.41 -1.7 206,256 - ABZR87_RS01030 0.50 +0.1
Or see this region's nucleotide sequence