Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS01010 and ABZR87_RS01015 are separated by 56 nucleotides ABZR87_RS01015 and ABZR87_RS01020 overlap by 8 nucleotides ABZR87_RS01020 and ABZR87_RS01025 are separated by 2 nucleotides
ABZR87_RS01010: ABZR87_RS01010 - ferrochelatase, at 200,437 to 201,552
_RS01010
ABZR87_RS01015: ABZR87_RS01015 - imidazolonepropionase, at 201,609 to 202,844
_RS01015
ABZR87_RS01020: ABZR87_RS01020 - formimidoylglutamase, at 202,837 to 203,814
_RS01020
ABZR87_RS01025: ABZR87_RS01025 - histidine ammonia-lyase, at 203,817 to 205,373
_RS01025
Position (kb) 201
202
203 Strain fitness (log2 ratio) -2
-1
0
1 at 201.570 kb on - strand at 201.582 kb on + strand at 201.582 kb on + strand at 201.584 kb on + strand at 201.584 kb on + strand at 201.584 kb on + strand at 201.584 kb on + strand at 201.585 kb on - strand at 201.585 kb on - strand at 201.585 kb on - strand at 201.610 kb on + strand at 201.610 kb on + strand at 201.610 kb on + strand at 201.610 kb on + strand at 201.611 kb on - strand at 201.611 kb on - strand at 201.667 kb on + strand at 201.667 kb on + strand at 201.667 kb on + strand at 201.867 kb on - strand, within ABZR87_RS01015 at 201.867 kb on - strand, within ABZR87_RS01015 at 201.955 kb on + strand, within ABZR87_RS01015 at 201.956 kb on - strand, within ABZR87_RS01015 at 201.956 kb on - strand, within ABZR87_RS01015 at 201.956 kb on - strand, within ABZR87_RS01015 at 201.956 kb on - strand, within ABZR87_RS01015 at 201.956 kb on - strand, within ABZR87_RS01015 at 202.257 kb on + strand, within ABZR87_RS01015 at 202.261 kb on + strand, within ABZR87_RS01015 at 202.261 kb on + strand, within ABZR87_RS01015 at 202.261 kb on + strand, within ABZR87_RS01015 at 202.261 kb on + strand, within ABZR87_RS01015 at 202.261 kb on + strand, within ABZR87_RS01015 at 202.261 kb on + strand, within ABZR87_RS01015 at 202.261 kb on + strand, within ABZR87_RS01015 at 202.261 kb on + strand, within ABZR87_RS01015 at 202.261 kb on + strand, within ABZR87_RS01015 at 202.261 kb on + strand, within ABZR87_RS01015 at 202.262 kb on - strand, within ABZR87_RS01015 at 202.262 kb on - strand, within ABZR87_RS01015 at 202.549 kb on + strand, within ABZR87_RS01015 at 202.549 kb on + strand, within ABZR87_RS01015 at 202.549 kb on + strand, within ABZR87_RS01015 at 202.549 kb on + strand, within ABZR87_RS01015 at 202.601 kb on - strand, within ABZR87_RS01015 at 203.090 kb on + strand, within ABZR87_RS01020 at 203.091 kb on - strand, within ABZR87_RS01020 at 203.228 kb on + strand, within ABZR87_RS01020 at 203.228 kb on + strand, within ABZR87_RS01020 at 203.228 kb on + strand, within ABZR87_RS01020 at 203.229 kb on - strand, within ABZR87_RS01020 at 203.229 kb on - strand, within ABZR87_RS01020 at 203.229 kb on - strand, within ABZR87_RS01020 at 203.252 kb on + strand, within ABZR87_RS01020 at 203.252 kb on + strand, within ABZR87_RS01020 at 203.253 kb on - strand, within ABZR87_RS01020 at 203.341 kb on + strand, within ABZR87_RS01020 at 203.370 kb on + strand, within ABZR87_RS01020
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 201,570 - +0.2 201,582 + -1.2 201,582 + -0.4 201,584 + -0.5 201,584 + -0.7 201,584 + -0.6 201,584 + +0.6 201,585 - -0.1 201,585 - +0.9 201,585 - +0.3 201,610 + +1.3 201,610 + -0.2 201,610 + -0.3 201,610 + +0.4 201,611 - +1.4 201,611 - +0.6 201,667 + -0.3 201,667 + +1.0 201,667 + -1.1 201,867 - ABZR87_RS01015 0.21 -0.4 201,867 - ABZR87_RS01015 0.21 -1.3 201,955 + ABZR87_RS01015 0.28 +0.1 201,956 - ABZR87_RS01015 0.28 +0.6 201,956 - ABZR87_RS01015 0.28 -0.8 201,956 - ABZR87_RS01015 0.28 -0.8 201,956 - ABZR87_RS01015 0.28 -0.1 201,956 - ABZR87_RS01015 0.28 -1.5 202,257 + ABZR87_RS01015 0.52 -0.4 202,261 + ABZR87_RS01015 0.53 +0.4 202,261 + ABZR87_RS01015 0.53 -1.2 202,261 + ABZR87_RS01015 0.53 -1.2 202,261 + ABZR87_RS01015 0.53 -2.1 202,261 + ABZR87_RS01015 0.53 +1.1 202,261 + ABZR87_RS01015 0.53 +1.0 202,261 + ABZR87_RS01015 0.53 -0.3 202,261 + ABZR87_RS01015 0.53 -0.4 202,261 + ABZR87_RS01015 0.53 -1.3 202,261 + ABZR87_RS01015 0.53 -2.7 202,262 - ABZR87_RS01015 0.53 +0.9 202,262 - ABZR87_RS01015 0.53 -0.7 202,549 + ABZR87_RS01015 0.76 -0.3 202,549 + ABZR87_RS01015 0.76 +0.2 202,549 + ABZR87_RS01015 0.76 +0.1 202,549 + ABZR87_RS01015 0.76 -0.8 202,601 - ABZR87_RS01015 0.80 +0.5 203,090 + ABZR87_RS01020 0.26 -0.6 203,091 - ABZR87_RS01020 0.26 -0.2 203,228 + ABZR87_RS01020 0.40 +0.0 203,228 + ABZR87_RS01020 0.40 -1.3 203,228 + ABZR87_RS01020 0.40 +0.7 203,229 - ABZR87_RS01020 0.40 -1.3 203,229 - ABZR87_RS01020 0.40 -0.6 203,229 - ABZR87_RS01020 0.40 -0.4 203,252 + ABZR87_RS01020 0.42 +1.5 203,252 + ABZR87_RS01020 0.42 -0.2 203,253 - ABZR87_RS01020 0.43 +0.2 203,341 + ABZR87_RS01020 0.52 -1.0 203,370 + ABZR87_RS01020 0.54 +0.6
Or see this region's nucleotide sequence