Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS00550 and ABZR87_RS00555 are separated by 7 nucleotides ABZR87_RS00555 and ABZR87_RS00560 are separated by 138 nucleotides ABZR87_RS00560 and ABZR87_RS00565 are separated by 13 nucleotides ABZR87_RS00565 and ABZR87_RS00570 are separated by 58 nucleotides
ABZR87_RS00550: ABZR87_RS00550 - neutral zinc metallopeptidase, at 113,146 to 114,030
_RS00550
ABZR87_RS00555: ABZR87_RS00555 - SDR family oxidoreductase, at 114,038 to 114,784
_RS00555
ABZR87_RS00560: ABZR87_RS00560 - DNA-3-methyladenine glycosylase 2, at 114,923 to 116,398
_RS00560
ABZR87_RS00565: ABZR87_RS00565 - methylated-DNA--[protein]-cysteine S-methyltransferase, at 116,412 to 116,960
_RS00565
ABZR87_RS00570: ABZR87_RS00570 - DMT family transporter, at 117,019 to 117,951
_RS00570
Position (kb)
114
115
116
117 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 113.956 kb on + strand at 113.956 kb on + strand at 113.956 kb on + strand at 113.957 kb on - strand at 113.957 kb on - strand at 113.957 kb on - strand at 113.957 kb on - strand at 113.957 kb on - strand at 113.957 kb on - strand at 113.957 kb on - strand at 114.078 kb on + strand at 114.078 kb on + strand at 114.078 kb on + strand at 114.078 kb on + strand at 114.078 kb on + strand at 114.079 kb on - strand at 114.079 kb on - strand at 114.205 kb on + strand, within ABZR87_RS00555 at 114.205 kb on + strand, within ABZR87_RS00555 at 114.206 kb on - strand, within ABZR87_RS00555 at 114.289 kb on + strand, within ABZR87_RS00555 at 114.290 kb on - strand, within ABZR87_RS00555 at 114.316 kb on - strand, within ABZR87_RS00555 at 114.316 kb on - strand, within ABZR87_RS00555 at 114.316 kb on - strand, within ABZR87_RS00555 at 114.324 kb on + strand, within ABZR87_RS00555 at 114.324 kb on + strand, within ABZR87_RS00555 at 114.324 kb on + strand, within ABZR87_RS00555 at 114.324 kb on + strand, within ABZR87_RS00555 at 114.324 kb on + strand, within ABZR87_RS00555 at 114.324 kb on + strand, within ABZR87_RS00555 at 114.325 kb on - strand, within ABZR87_RS00555 at 114.325 kb on - strand, within ABZR87_RS00555 at 114.325 kb on - strand, within ABZR87_RS00555 at 114.325 kb on - strand, within ABZR87_RS00555 at 114.325 kb on - strand, within ABZR87_RS00555 at 114.325 kb on - strand, within ABZR87_RS00555 at 114.325 kb on - strand, within ABZR87_RS00555 at 114.325 kb on - strand, within ABZR87_RS00555 at 114.325 kb on - strand, within ABZR87_RS00555 at 114.602 kb on + strand, within ABZR87_RS00555 at 114.602 kb on + strand, within ABZR87_RS00555 at 114.602 kb on + strand, within ABZR87_RS00555 at 114.603 kb on - strand, within ABZR87_RS00555 at 114.603 kb on - strand, within ABZR87_RS00555 at 114.603 kb on - strand, within ABZR87_RS00555 at 114.603 kb on - strand, within ABZR87_RS00555 at 114.603 kb on - strand, within ABZR87_RS00555 at 114.603 kb on - strand, within ABZR87_RS00555 at 114.746 kb on - strand at 114.828 kb on + strand at 114.891 kb on + strand at 114.892 kb on - strand at 114.892 kb on - strand at 114.896 kb on + strand at 114.897 kb on - strand at 114.897 kb on - strand at 114.897 kb on - strand at 114.897 kb on - strand at 114.897 kb on - strand at 114.947 kb on + strand at 114.947 kb on + strand at 114.947 kb on + strand at 114.948 kb on - strand at 115.017 kb on + strand at 115.017 kb on + strand at 115.017 kb on + strand at 115.018 kb on - strand at 115.018 kb on - strand at 115.018 kb on - strand at 115.018 kb on - strand at 115.018 kb on - strand at 115.019 kb on + strand at 115.019 kb on + strand at 115.019 kb on + strand at 115.020 kb on - strand at 115.020 kb on - strand at 115.020 kb on - strand at 115.316 kb on + strand, within ABZR87_RS00560 at 115.435 kb on + strand, within ABZR87_RS00560 at 115.611 kb on - strand, within ABZR87_RS00560 at 115.611 kb on - strand, within ABZR87_RS00560 at 115.611 kb on - strand, within ABZR87_RS00560 at 115.635 kb on - strand, within ABZR87_RS00560 at 115.943 kb on + strand, within ABZR87_RS00560 at 115.943 kb on + strand, within ABZR87_RS00560 at 115.944 kb on - strand, within ABZR87_RS00560 at 115.944 kb on - strand, within ABZR87_RS00560 at 116.192 kb on + strand, within ABZR87_RS00560 at 116.192 kb on + strand, within ABZR87_RS00560 at 116.192 kb on + strand, within ABZR87_RS00560 at 116.192 kb on + strand, within ABZR87_RS00560 at 116.192 kb on + strand, within ABZR87_RS00560 at 116.192 kb on + strand, within ABZR87_RS00560 at 116.193 kb on - strand, within ABZR87_RS00560 at 116.193 kb on - strand, within ABZR87_RS00560 at 116.193 kb on - strand, within ABZR87_RS00560 at 116.193 kb on - strand, within ABZR87_RS00560 at 116.193 kb on - strand, within ABZR87_RS00560 at 116.243 kb on + strand, within ABZR87_RS00560 at 116.243 kb on + strand, within ABZR87_RS00560 at 116.243 kb on + strand, within ABZR87_RS00560 at 116.244 kb on - strand, within ABZR87_RS00560 at 116.340 kb on - strand at 116.340 kb on - strand at 116.415 kb on + strand at 116.416 kb on - strand at 116.416 kb on - strand at 116.586 kb on + strand, within ABZR87_RS00565 at 116.587 kb on - strand, within ABZR87_RS00565 at 116.587 kb on - strand, within ABZR87_RS00565 at 116.587 kb on - strand, within ABZR87_RS00565 at 116.700 kb on + strand, within ABZR87_RS00565 at 116.700 kb on + strand, within ABZR87_RS00565 at 116.700 kb on + strand, within ABZR87_RS00565 at 116.700 kb on + strand, within ABZR87_RS00565 at 116.700 kb on + strand, within ABZR87_RS00565 at 116.700 kb on + strand, within ABZR87_RS00565 at 116.701 kb on - strand, within ABZR87_RS00565 at 116.701 kb on - strand, within ABZR87_RS00565 at 116.701 kb on - strand, within ABZR87_RS00565 at 116.701 kb on - strand, within ABZR87_RS00565 at 116.701 kb on - strand, within ABZR87_RS00565 at 116.958 kb on + strand at 116.958 kb on + strand at 117.116 kb on + strand, within ABZR87_RS00570 at 117.116 kb on + strand, within ABZR87_RS00570 at 117.279 kb on + strand, within ABZR87_RS00570 at 117.279 kb on + strand, within ABZR87_RS00570 at 117.280 kb on - strand, within ABZR87_RS00570 at 117.297 kb on + strand, within ABZR87_RS00570 at 117.298 kb on - strand, within ABZR87_RS00570 at 117.298 kb on - strand, within ABZR87_RS00570 at 117.298 kb on - strand, within ABZR87_RS00570 at 117.298 kb on - strand, within ABZR87_RS00570 at 117.298 kb on - strand, within ABZR87_RS00570 at 117.348 kb on + strand, within ABZR87_RS00570 at 117.348 kb on + strand, within ABZR87_RS00570 at 117.348 kb on + strand, within ABZR87_RS00570 at 117.348 kb on + strand, within ABZR87_RS00570 at 117.348 kb on + strand, within ABZR87_RS00570 at 117.349 kb on - strand, within ABZR87_RS00570 at 117.349 kb on - strand, within ABZR87_RS00570 at 117.349 kb on - strand, within ABZR87_RS00570 at 117.349 kb on - strand, within ABZR87_RS00570 at 117.349 kb on - strand, within ABZR87_RS00570 at 117.349 kb on - strand, within ABZR87_RS00570
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 113,956 + -0.5 113,956 + +0.6 113,956 + -2.0 113,957 - -0.7 113,957 - -0.4 113,957 - +0.1 113,957 - +1.7 113,957 - +0.1 113,957 - +0.3 113,957 - +0.8 114,078 + -1.0 114,078 + -0.7 114,078 + -0.2 114,078 + -0.0 114,078 + +0.3 114,079 - +0.0 114,079 - -0.3 114,205 + ABZR87_RS00555 0.22 -0.3 114,205 + ABZR87_RS00555 0.22 +1.0 114,206 - ABZR87_RS00555 0.22 +0.7 114,289 + ABZR87_RS00555 0.34 +0.5 114,290 - ABZR87_RS00555 0.34 +0.1 114,316 - ABZR87_RS00555 0.37 -0.7 114,316 - ABZR87_RS00555 0.37 +0.7 114,316 - ABZR87_RS00555 0.37 +0.3 114,324 + ABZR87_RS00555 0.38 +0.1 114,324 + ABZR87_RS00555 0.38 -0.4 114,324 + ABZR87_RS00555 0.38 -0.6 114,324 + ABZR87_RS00555 0.38 -0.0 114,324 + ABZR87_RS00555 0.38 +0.4 114,324 + ABZR87_RS00555 0.38 +1.6 114,325 - ABZR87_RS00555 0.38 +0.7 114,325 - ABZR87_RS00555 0.38 +0.8 114,325 - ABZR87_RS00555 0.38 +0.6 114,325 - ABZR87_RS00555 0.38 -1.0 114,325 - ABZR87_RS00555 0.38 -0.1 114,325 - ABZR87_RS00555 0.38 +0.3 114,325 - ABZR87_RS00555 0.38 +0.2 114,325 - ABZR87_RS00555 0.38 +0.1 114,325 - ABZR87_RS00555 0.38 -0.4 114,602 + ABZR87_RS00555 0.76 -0.2 114,602 + ABZR87_RS00555 0.76 -0.8 114,602 + ABZR87_RS00555 0.76 +0.4 114,603 - ABZR87_RS00555 0.76 -0.7 114,603 - ABZR87_RS00555 0.76 -1.9 114,603 - ABZR87_RS00555 0.76 -0.4 114,603 - ABZR87_RS00555 0.76 +0.3 114,603 - ABZR87_RS00555 0.76 +1.5 114,603 - ABZR87_RS00555 0.76 +0.6 114,746 - +0.1 114,828 + -1.0 114,891 + -1.8 114,892 - -1.4 114,892 - -0.5 114,896 + +0.7 114,897 - -0.6 114,897 - +1.1 114,897 - +0.0 114,897 - +0.6 114,897 - +0.4 114,947 + +0.5 114,947 + -0.2 114,947 + +0.5 114,948 - -0.0 115,017 + -0.1 115,017 + +1.9 115,017 + -0.1 115,018 - +0.7 115,018 - +0.2 115,018 - +0.5 115,018 - -0.4 115,018 - -0.1 115,019 + -0.9 115,019 + -0.9 115,019 + -0.3 115,020 - +0.6 115,020 - -0.3 115,020 - -0.4 115,316 + ABZR87_RS00560 0.27 +1.5 115,435 + ABZR87_RS00560 0.35 -0.9 115,611 - ABZR87_RS00560 0.47 -0.1 115,611 - ABZR87_RS00560 0.47 -0.3 115,611 - ABZR87_RS00560 0.47 -1.2 115,635 - ABZR87_RS00560 0.48 +1.2 115,943 + ABZR87_RS00560 0.69 -0.6 115,943 + ABZR87_RS00560 0.69 -0.2 115,944 - ABZR87_RS00560 0.69 -0.6 115,944 - ABZR87_RS00560 0.69 +0.1 116,192 + ABZR87_RS00560 0.86 -0.2 116,192 + ABZR87_RS00560 0.86 +0.5 116,192 + ABZR87_RS00560 0.86 -0.8 116,192 + ABZR87_RS00560 0.86 +0.3 116,192 + ABZR87_RS00560 0.86 -0.3 116,192 + ABZR87_RS00560 0.86 +0.1 116,193 - ABZR87_RS00560 0.86 +0.4 116,193 - ABZR87_RS00560 0.86 -1.4 116,193 - ABZR87_RS00560 0.86 +1.1 116,193 - ABZR87_RS00560 0.86 +0.1 116,193 - ABZR87_RS00560 0.86 +0.9 116,243 + ABZR87_RS00560 0.89 +0.1 116,243 + ABZR87_RS00560 0.89 +0.1 116,243 + ABZR87_RS00560 0.89 +0.3 116,244 - ABZR87_RS00560 0.89 -1.2 116,340 - +0.7 116,340 - -0.2 116,415 + +0.5 116,416 - +0.3 116,416 - -0.2 116,586 + ABZR87_RS00565 0.32 -0.6 116,587 - ABZR87_RS00565 0.32 +0.4 116,587 - ABZR87_RS00565 0.32 -0.5 116,587 - ABZR87_RS00565 0.32 -0.5 116,700 + ABZR87_RS00565 0.52 +0.6 116,700 + ABZR87_RS00565 0.52 -0.1 116,700 + ABZR87_RS00565 0.52 +0.4 116,700 + ABZR87_RS00565 0.52 +0.2 116,700 + ABZR87_RS00565 0.52 -0.8 116,700 + ABZR87_RS00565 0.52 +1.2 116,701 - ABZR87_RS00565 0.53 +0.3 116,701 - ABZR87_RS00565 0.53 -0.0 116,701 - ABZR87_RS00565 0.53 +1.1 116,701 - ABZR87_RS00565 0.53 -0.7 116,701 - ABZR87_RS00565 0.53 -0.4 116,958 + +0.3 116,958 + -0.4 117,116 + ABZR87_RS00570 0.10 -0.0 117,116 + ABZR87_RS00570 0.10 -1.5 117,279 + ABZR87_RS00570 0.28 +0.3 117,279 + ABZR87_RS00570 0.28 +0.4 117,280 - ABZR87_RS00570 0.28 -0.2 117,297 + ABZR87_RS00570 0.30 -1.7 117,298 - ABZR87_RS00570 0.30 +0.8 117,298 - ABZR87_RS00570 0.30 -0.4 117,298 - ABZR87_RS00570 0.30 +0.6 117,298 - ABZR87_RS00570 0.30 +1.8 117,298 - ABZR87_RS00570 0.30 -0.5 117,348 + ABZR87_RS00570 0.35 +0.5 117,348 + ABZR87_RS00570 0.35 -0.3 117,348 + ABZR87_RS00570 0.35 -0.3 117,348 + ABZR87_RS00570 0.35 +0.1 117,348 + ABZR87_RS00570 0.35 +0.3 117,349 - ABZR87_RS00570 0.35 -1.3 117,349 - ABZR87_RS00570 0.35 -0.3 117,349 - ABZR87_RS00570 0.35 +1.3 117,349 - ABZR87_RS00570 0.35 +0.1 117,349 - ABZR87_RS00570 0.35 +2.5 117,349 - ABZR87_RS00570 0.35 -0.6
Or see this region's nucleotide sequence