Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS23170 and ABZR87_RS23175 are separated by 11 nucleotides ABZR87_RS23175 and ABZR87_RS23180 overlap by 4 nucleotides ABZR87_RS23180 and ABZR87_RS23185 overlap by 4 nucleotides ABZR87_RS23185 and ABZR87_RS23190 overlap by 1 nucleotides
ABZR87_RS23170: ABZR87_RS23170 - Zn-dependent hydrolase, at 1,561,536 to 1,562,789
_RS23170
ABZR87_RS23175: ABZR87_RS23175 - ABC transporter ATP-binding protein, at 1,562,801 to 1,563,517
_RS23175
ABZR87_RS23180: ABZR87_RS23180 - ABC transporter ATP-binding protein, at 1,563,514 to 1,564,284
_RS23180
ABZR87_RS23185: ABZR87_RS23185 - branched-chain amino acid ABC transporter permease, at 1,564,281 to 1,565,249
_RS23185
ABZR87_RS23190: ABZR87_RS23190 - branched-chain amino acid ABC transporter permease, at 1,565,249 to 1,566,106
_RS23190
Position (kb)
1563
1564
1565 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1562.522 kb on + strand, within ABZR87_RS23170 at 1562.796 kb on + strand at 1562.797 kb on - strand at 1562.797 kb on - strand at 1562.826 kb on + strand at 1562.826 kb on + strand at 1562.878 kb on - strand, within ABZR87_RS23175 at 1562.878 kb on - strand, within ABZR87_RS23175 at 1563.165 kb on + strand, within ABZR87_RS23175 at 1563.165 kb on + strand, within ABZR87_RS23175 at 1563.165 kb on + strand, within ABZR87_RS23175 at 1563.472 kb on - strand at 1563.474 kb on + strand at 1563.961 kb on - strand, within ABZR87_RS23180 at 1564.059 kb on - strand, within ABZR87_RS23180 at 1564.238 kb on + strand at 1564.459 kb on + strand, within ABZR87_RS23185 at 1564.459 kb on + strand, within ABZR87_RS23185 at 1564.459 kb on + strand, within ABZR87_RS23185 at 1564.459 kb on + strand, within ABZR87_RS23185 at 1564.460 kb on - strand, within ABZR87_RS23185 at 1564.460 kb on - strand, within ABZR87_RS23185 at 1564.460 kb on - strand, within ABZR87_RS23185 at 1564.543 kb on + strand, within ABZR87_RS23185 at 1564.544 kb on - strand, within ABZR87_RS23185 at 1564.544 kb on - strand, within ABZR87_RS23185 at 1564.551 kb on - strand, within ABZR87_RS23185 at 1564.781 kb on - strand, within ABZR87_RS23185 at 1564.781 kb on - strand, within ABZR87_RS23185 at 1564.864 kb on + strand, within ABZR87_RS23185 at 1564.865 kb on - strand, within ABZR87_RS23185 at 1564.865 kb on - strand, within ABZR87_RS23185 at 1564.877 kb on - strand, within ABZR87_RS23185 at 1564.877 kb on - strand, within ABZR87_RS23185 at 1564.877 kb on - strand, within ABZR87_RS23185 at 1564.915 kb on + strand, within ABZR87_RS23185 at 1564.915 kb on + strand, within ABZR87_RS23185 at 1564.916 kb on - strand, within ABZR87_RS23185 at 1564.916 kb on - strand, within ABZR87_RS23185 at 1564.916 kb on - strand, within ABZR87_RS23185 at 1564.916 kb on - strand, within ABZR87_RS23185 at 1565.041 kb on + strand, within ABZR87_RS23185 at 1565.042 kb on - strand, within ABZR87_RS23185 at 1565.059 kb on + strand, within ABZR87_RS23185 at 1565.092 kb on + strand, within ABZR87_RS23185 at 1565.131 kb on + strand, within ABZR87_RS23185 at 1565.132 kb on - strand, within ABZR87_RS23185 at 1565.139 kb on + strand, within ABZR87_RS23185 at 1565.140 kb on - strand, within ABZR87_RS23185 at 1565.230 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 1,562,522 + ABZR87_RS23170 0.79 -0.8 1,562,796 + -2.2 1,562,797 - -1.0 1,562,797 - -0.2 1,562,826 + +0.3 1,562,826 + -0.1 1,562,878 - ABZR87_RS23175 0.11 +0.1 1,562,878 - ABZR87_RS23175 0.11 +0.4 1,563,165 + ABZR87_RS23175 0.51 +0.5 1,563,165 + ABZR87_RS23175 0.51 -0.4 1,563,165 + ABZR87_RS23175 0.51 +0.9 1,563,472 - +0.0 1,563,474 + +0.6 1,563,961 - ABZR87_RS23180 0.58 -1.7 1,564,059 - ABZR87_RS23180 0.71 -0.4 1,564,238 + -0.3 1,564,459 + ABZR87_RS23185 0.18 +1.2 1,564,459 + ABZR87_RS23185 0.18 -0.2 1,564,459 + ABZR87_RS23185 0.18 -0.7 1,564,459 + ABZR87_RS23185 0.18 -1.7 1,564,460 - ABZR87_RS23185 0.18 +0.2 1,564,460 - ABZR87_RS23185 0.18 +0.4 1,564,460 - ABZR87_RS23185 0.18 +1.9 1,564,543 + ABZR87_RS23185 0.27 +0.1 1,564,544 - ABZR87_RS23185 0.27 +0.5 1,564,544 - ABZR87_RS23185 0.27 +0.1 1,564,551 - ABZR87_RS23185 0.28 -0.0 1,564,781 - ABZR87_RS23185 0.52 +0.2 1,564,781 - ABZR87_RS23185 0.52 +0.3 1,564,864 + ABZR87_RS23185 0.60 -0.1 1,564,865 - ABZR87_RS23185 0.60 -0.4 1,564,865 - ABZR87_RS23185 0.60 -0.4 1,564,877 - ABZR87_RS23185 0.62 -0.3 1,564,877 - ABZR87_RS23185 0.62 -0.7 1,564,877 - ABZR87_RS23185 0.62 -0.1 1,564,915 + ABZR87_RS23185 0.65 +0.1 1,564,915 + ABZR87_RS23185 0.65 +0.6 1,564,916 - ABZR87_RS23185 0.66 -0.8 1,564,916 - ABZR87_RS23185 0.66 +0.3 1,564,916 - ABZR87_RS23185 0.66 +0.3 1,564,916 - ABZR87_RS23185 0.66 -1.0 1,565,041 + ABZR87_RS23185 0.78 +0.5 1,565,042 - ABZR87_RS23185 0.79 +0.3 1,565,059 + ABZR87_RS23185 0.80 +0.2 1,565,092 + ABZR87_RS23185 0.84 +0.1 1,565,131 + ABZR87_RS23185 0.88 +0.3 1,565,132 - ABZR87_RS23185 0.88 +0.0 1,565,139 + ABZR87_RS23185 0.89 -0.5 1,565,140 - ABZR87_RS23185 0.89 -0.4 1,565,230 - +0.4
Or see this region's nucleotide sequence