Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS22250 and ABZR87_RS22255 are separated by 16 nucleotides ABZR87_RS22255 and ABZR87_RS22260 are separated by 10 nucleotides ABZR87_RS22260 and ABZR87_RS22265 are separated by 33 nucleotides
ABZR87_RS22250: ABZR87_RS22250 - flagellar assembly peptidoglycan hydrolase FlgJ, at 1,353,499 to 1,354,584
_RS22250
ABZR87_RS22255: ABZR87_RS22255 - flagellar basal body P-ring protein FlgI, at 1,354,601 to 1,355,719
_RS22255
ABZR87_RS22260: ABZR87_RS22260 - flagellar basal body L-ring protein FlgH, at 1,355,730 to 1,356,464
_RS22260
ABZR87_RS22265: ABZR87_RS22265 - flagellar basal-body rod protein FlgG, at 1,356,498 to 1,357,280
_RS22265
Position (kb)
1354
1355
1356 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1353.659 kb on + strand, within ABZR87_RS22250 at 1353.659 kb on + strand, within ABZR87_RS22250 at 1353.659 kb on + strand, within ABZR87_RS22250 at 1353.660 kb on - strand, within ABZR87_RS22250 at 1353.690 kb on - strand, within ABZR87_RS22250 at 1353.734 kb on + strand, within ABZR87_RS22250 at 1353.734 kb on + strand, within ABZR87_RS22250 at 1353.735 kb on - strand, within ABZR87_RS22250 at 1353.744 kb on + strand, within ABZR87_RS22250 at 1353.790 kb on - strand, within ABZR87_RS22250 at 1353.790 kb on - strand, within ABZR87_RS22250 at 1353.939 kb on - strand, within ABZR87_RS22250 at 1353.939 kb on - strand, within ABZR87_RS22250 at 1353.939 kb on - strand, within ABZR87_RS22250 at 1354.218 kb on + strand, within ABZR87_RS22250 at 1354.218 kb on + strand, within ABZR87_RS22250 at 1354.219 kb on - strand, within ABZR87_RS22250 at 1354.219 kb on - strand, within ABZR87_RS22250 at 1354.379 kb on + strand, within ABZR87_RS22250 at 1354.379 kb on + strand, within ABZR87_RS22250 at 1354.379 kb on + strand, within ABZR87_RS22250 at 1354.379 kb on + strand, within ABZR87_RS22250 at 1354.379 kb on + strand, within ABZR87_RS22250 at 1354.380 kb on - strand, within ABZR87_RS22250 at 1354.380 kb on - strand, within ABZR87_RS22250 at 1354.380 kb on - strand, within ABZR87_RS22250 at 1354.509 kb on + strand at 1354.509 kb on + strand at 1354.509 kb on + strand at 1354.509 kb on + strand at 1354.509 kb on + strand at 1354.510 kb on - strand at 1354.510 kb on - strand at 1354.510 kb on - strand at 1354.510 kb on - strand at 1355.098 kb on + strand, within ABZR87_RS22255 at 1355.295 kb on + strand, within ABZR87_RS22255 at 1355.295 kb on + strand, within ABZR87_RS22255 at 1355.295 kb on + strand, within ABZR87_RS22255 at 1355.295 kb on + strand, within ABZR87_RS22255 at 1355.510 kb on - strand, within ABZR87_RS22255 at 1355.794 kb on + strand at 1355.794 kb on + strand at 1355.795 kb on - strand at 1355.795 kb on - strand at 1355.795 kb on - strand at 1355.809 kb on + strand, within ABZR87_RS22260 at 1355.985 kb on - strand, within ABZR87_RS22260 at 1356.176 kb on + strand, within ABZR87_RS22260 at 1356.371 kb on + strand, within ABZR87_RS22260 at 1356.414 kb on - strand at 1356.414 kb on - strand at 1356.414 kb on - strand at 1356.458 kb on + strand at 1356.459 kb on - strand at 1356.459 kb on - strand at 1356.499 kb on + strand at 1356.499 kb on + strand at 1356.499 kb on + strand at 1356.500 kb on - strand at 1356.500 kb on - strand at 1356.500 kb on - strand at 1356.563 kb on - strand at 1356.628 kb on + strand, within ABZR87_RS22265 at 1356.628 kb on + strand, within ABZR87_RS22265 at 1356.628 kb on + strand, within ABZR87_RS22265 at 1356.628 kb on + strand, within ABZR87_RS22265 at 1356.629 kb on - strand, within ABZR87_RS22265
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 1,353,659 + ABZR87_RS22250 0.15 -1.3 1,353,659 + ABZR87_RS22250 0.15 -1.0 1,353,659 + ABZR87_RS22250 0.15 +0.1 1,353,660 - ABZR87_RS22250 0.15 -0.5 1,353,690 - ABZR87_RS22250 0.18 -1.0 1,353,734 + ABZR87_RS22250 0.22 +0.7 1,353,734 + ABZR87_RS22250 0.22 -0.4 1,353,735 - ABZR87_RS22250 0.22 -1.1 1,353,744 + ABZR87_RS22250 0.23 +0.0 1,353,790 - ABZR87_RS22250 0.27 -0.8 1,353,790 - ABZR87_RS22250 0.27 +0.2 1,353,939 - ABZR87_RS22250 0.41 +0.1 1,353,939 - ABZR87_RS22250 0.41 -1.4 1,353,939 - ABZR87_RS22250 0.41 +1.1 1,354,218 + ABZR87_RS22250 0.66 +0.1 1,354,218 + ABZR87_RS22250 0.66 -0.0 1,354,219 - ABZR87_RS22250 0.66 -0.0 1,354,219 - ABZR87_RS22250 0.66 -0.2 1,354,379 + ABZR87_RS22250 0.81 -0.4 1,354,379 + ABZR87_RS22250 0.81 -1.1 1,354,379 + ABZR87_RS22250 0.81 -2.4 1,354,379 + ABZR87_RS22250 0.81 -0.1 1,354,379 + ABZR87_RS22250 0.81 -0.8 1,354,380 - ABZR87_RS22250 0.81 -0.6 1,354,380 - ABZR87_RS22250 0.81 -1.6 1,354,380 - ABZR87_RS22250 0.81 -3.4 1,354,509 + -2.2 1,354,509 + -1.0 1,354,509 + +0.2 1,354,509 + -0.6 1,354,509 + -1.3 1,354,510 - -1.1 1,354,510 - -2.7 1,354,510 - -0.6 1,354,510 - +0.4 1,355,098 + ABZR87_RS22255 0.44 -2.1 1,355,295 + ABZR87_RS22255 0.62 +0.4 1,355,295 + ABZR87_RS22255 0.62 -1.3 1,355,295 + ABZR87_RS22255 0.62 -0.4 1,355,295 + ABZR87_RS22255 0.62 -1.2 1,355,510 - ABZR87_RS22255 0.81 -0.0 1,355,794 + +0.0 1,355,794 + -0.1 1,355,795 - -1.2 1,355,795 - -0.5 1,355,795 - +2.1 1,355,809 + ABZR87_RS22260 0.11 -1.1 1,355,985 - ABZR87_RS22260 0.35 -0.4 1,356,176 + ABZR87_RS22260 0.61 -0.3 1,356,371 + ABZR87_RS22260 0.87 -2.0 1,356,414 - +0.2 1,356,414 - +0.0 1,356,414 - -0.4 1,356,458 + +0.4 1,356,459 - -0.3 1,356,459 - +0.1 1,356,499 + -1.0 1,356,499 + -0.8 1,356,499 + -0.4 1,356,500 - -0.2 1,356,500 - +0.4 1,356,500 - -0.4 1,356,563 - -0.7 1,356,628 + ABZR87_RS22265 0.17 -0.6 1,356,628 + ABZR87_RS22265 0.17 -0.9 1,356,628 + ABZR87_RS22265 0.17 -0.3 1,356,628 + ABZR87_RS22265 0.17 -2.0 1,356,629 - ABZR87_RS22265 0.17 -0.8
Or see this region's nucleotide sequence