Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS22100 and ABZR87_RS22105 overlap by 4 nucleotides ABZR87_RS22105 and ABZR87_RS22110 overlap by 4 nucleotides
ABZR87_RS22100: ABZR87_RS22100 - CDP-glucose 4,6-dehydratase, at 1,326,674 to 1,327,780
_RS22100
ABZR87_RS22105: ABZR87_RS22105 - dTDP-4-dehydrorhamnose 3,5-epimerase, at 1,327,777 to 1,328,370
_RS22105
ABZR87_RS22110: ABZR87_RS22110 - DegT/DnrJ/EryC1/StrS family aminotransferase, at 1,328,367 to 1,329,518
_RS22110
Position (kb)
1327
1328
1329 Strain fitness (log2 ratio)
-1
0
1 at 1326.804 kb on - strand, within ABZR87_RS22100 at 1326.838 kb on + strand, within ABZR87_RS22100 at 1326.838 kb on + strand, within ABZR87_RS22100 at 1326.980 kb on + strand, within ABZR87_RS22100 at 1326.981 kb on - strand, within ABZR87_RS22100 at 1326.981 kb on - strand, within ABZR87_RS22100 at 1327.001 kb on + strand, within ABZR87_RS22100 at 1327.001 kb on + strand, within ABZR87_RS22100 at 1327.001 kb on + strand, within ABZR87_RS22100 at 1327.001 kb on + strand, within ABZR87_RS22100 at 1327.002 kb on - strand, within ABZR87_RS22100 at 1327.098 kb on - strand, within ABZR87_RS22100 at 1327.160 kb on + strand, within ABZR87_RS22100 at 1327.160 kb on + strand, within ABZR87_RS22100 at 1327.161 kb on - strand, within ABZR87_RS22100 at 1327.161 kb on - strand, within ABZR87_RS22100 at 1327.294 kb on + strand, within ABZR87_RS22100 at 1327.295 kb on - strand, within ABZR87_RS22100 at 1327.295 kb on - strand, within ABZR87_RS22100 at 1327.397 kb on + strand, within ABZR87_RS22100 at 1327.398 kb on - strand, within ABZR87_RS22100 at 1327.398 kb on - strand, within ABZR87_RS22100 at 1327.673 kb on + strand at 1327.838 kb on + strand, within ABZR87_RS22105 at 1328.179 kb on + strand, within ABZR87_RS22105 at 1328.180 kb on - strand, within ABZR87_RS22105 at 1328.180 kb on - strand, within ABZR87_RS22105 at 1328.180 kb on - strand, within ABZR87_RS22105 at 1328.180 kb on - strand, within ABZR87_RS22105 at 1328.385 kb on + strand at 1328.422 kb on - strand at 1328.422 kb on - strand at 1328.463 kb on + strand at 1328.463 kb on + strand at 1328.463 kb on + strand at 1328.463 kb on + strand at 1328.464 kb on - strand at 1328.469 kb on + strand at 1328.469 kb on + strand at 1328.470 kb on - strand at 1328.470 kb on - strand at 1328.492 kb on - strand, within ABZR87_RS22110 at 1328.517 kb on + strand, within ABZR87_RS22110 at 1328.517 kb on + strand, within ABZR87_RS22110 at 1328.518 kb on - strand, within ABZR87_RS22110 at 1328.518 kb on - strand, within ABZR87_RS22110 at 1328.643 kb on + strand, within ABZR87_RS22110 at 1328.643 kb on + strand, within ABZR87_RS22110 at 1328.643 kb on + strand, within ABZR87_RS22110 at 1328.643 kb on + strand, within ABZR87_RS22110 at 1328.644 kb on - strand, within ABZR87_RS22110 at 1328.644 kb on - strand, within ABZR87_RS22110 at 1328.644 kb on - strand, within ABZR87_RS22110 at 1328.761 kb on - strand, within ABZR87_RS22110 at 1328.970 kb on + strand, within ABZR87_RS22110 at 1328.970 kb on + strand, within ABZR87_RS22110 at 1328.971 kb on - strand, within ABZR87_RS22110 at 1328.971 kb on - strand, within ABZR87_RS22110 at 1329.051 kb on + strand, within ABZR87_RS22110 at 1329.051 kb on + strand, within ABZR87_RS22110 at 1329.051 kb on + strand, within ABZR87_RS22110 at 1329.144 kb on + strand, within ABZR87_RS22110 at 1329.145 kb on - strand, within ABZR87_RS22110 at 1329.213 kb on + strand, within ABZR87_RS22110 at 1329.213 kb on + strand, within ABZR87_RS22110 at 1329.214 kb on - strand, within ABZR87_RS22110 at 1329.214 kb on - strand, within ABZR87_RS22110 at 1329.214 kb on - strand, within ABZR87_RS22110
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 1,326,804 - ABZR87_RS22100 0.12 -0.6 1,326,838 + ABZR87_RS22100 0.15 -1.4 1,326,838 + ABZR87_RS22100 0.15 -0.7 1,326,980 + ABZR87_RS22100 0.28 -0.3 1,326,981 - ABZR87_RS22100 0.28 -0.3 1,326,981 - ABZR87_RS22100 0.28 -0.6 1,327,001 + ABZR87_RS22100 0.30 +0.7 1,327,001 + ABZR87_RS22100 0.30 -0.0 1,327,001 + ABZR87_RS22100 0.30 +1.2 1,327,001 + ABZR87_RS22100 0.30 -1.1 1,327,002 - ABZR87_RS22100 0.30 +0.2 1,327,098 - ABZR87_RS22100 0.38 +0.1 1,327,160 + ABZR87_RS22100 0.44 +0.3 1,327,160 + ABZR87_RS22100 0.44 -0.9 1,327,161 - ABZR87_RS22100 0.44 +0.1 1,327,161 - ABZR87_RS22100 0.44 -0.6 1,327,294 + ABZR87_RS22100 0.56 -0.5 1,327,295 - ABZR87_RS22100 0.56 -1.1 1,327,295 - ABZR87_RS22100 0.56 -0.1 1,327,397 + ABZR87_RS22100 0.65 -0.5 1,327,398 - ABZR87_RS22100 0.65 +0.4 1,327,398 - ABZR87_RS22100 0.65 +0.5 1,327,673 + +1.6 1,327,838 + ABZR87_RS22105 0.10 -1.0 1,328,179 + ABZR87_RS22105 0.68 -0.3 1,328,180 - ABZR87_RS22105 0.68 -0.4 1,328,180 - ABZR87_RS22105 0.68 -1.0 1,328,180 - ABZR87_RS22105 0.68 -0.9 1,328,180 - ABZR87_RS22105 0.68 -0.7 1,328,385 + -0.0 1,328,422 - -1.0 1,328,422 - -0.1 1,328,463 + -0.5 1,328,463 + +0.3 1,328,463 + +0.3 1,328,463 + -0.3 1,328,464 - +0.1 1,328,469 + -0.1 1,328,469 + -0.6 1,328,470 - -0.1 1,328,470 - +0.3 1,328,492 - ABZR87_RS22110 0.11 +1.1 1,328,517 + ABZR87_RS22110 0.13 +1.2 1,328,517 + ABZR87_RS22110 0.13 -0.2 1,328,518 - ABZR87_RS22110 0.13 -0.8 1,328,518 - ABZR87_RS22110 0.13 -0.3 1,328,643 + ABZR87_RS22110 0.24 +0.1 1,328,643 + ABZR87_RS22110 0.24 +0.2 1,328,643 + ABZR87_RS22110 0.24 +1.2 1,328,643 + ABZR87_RS22110 0.24 -1.1 1,328,644 - ABZR87_RS22110 0.24 -0.1 1,328,644 - ABZR87_RS22110 0.24 +0.9 1,328,644 - ABZR87_RS22110 0.24 +0.8 1,328,761 - ABZR87_RS22110 0.34 +1.1 1,328,970 + ABZR87_RS22110 0.52 -0.4 1,328,970 + ABZR87_RS22110 0.52 -0.8 1,328,971 - ABZR87_RS22110 0.52 -0.3 1,328,971 - ABZR87_RS22110 0.52 -0.2 1,329,051 + ABZR87_RS22110 0.59 -0.3 1,329,051 + ABZR87_RS22110 0.59 -0.3 1,329,051 + ABZR87_RS22110 0.59 +0.1 1,329,144 + ABZR87_RS22110 0.67 -0.6 1,329,145 - ABZR87_RS22110 0.68 -0.2 1,329,213 + ABZR87_RS22110 0.73 -0.5 1,329,213 + ABZR87_RS22110 0.73 -0.7 1,329,214 - ABZR87_RS22110 0.74 +0.1 1,329,214 - ABZR87_RS22110 0.74 +0.4 1,329,214 - ABZR87_RS22110 0.74 +0.7
Or see this region's nucleotide sequence